Modeling shrimp protein and energy requirements for growth Submitted to Swansea University in fulfillment of the requirements for the Degree of MSc Environmental Biology 2012 Martin Richardson 578168 Martin Richardson 578168 Summary In addition to presenting a basic individual, factorial growth model for shrimp, this dissertation provides an overview of some of the ways that modeling and simulation have developed from single equation mathematical models to modern dynamic systems capable of integrating data from multiple experiments. A model may use results or data from several sources, and where necessary make assumptions. The model can then be tested by comparing the results to empirical data. Models attempt to analyze entire ecosystems and therefore seek to use the principle of conservation of energy, or stoichiometry as a means of integrating as many elements as possible in a consistent manner. This new paradigm allows for rapid evaluation of environmental problems at both large and small scale. Models can be used to analyze behavior of complex ecosystems and their interactions with a changing environment in a general way. Eventually they will be integrated with global climate models, abiotic resources and anthropogenic factors to develop a comprehensive model of the Biosphere. A model that can be played backwards or forwards, to predict the future or remember the past, as things were. Dynamic modeling provides a means of synthesizing disparate fields associated with global issues, particularly economics, ecology and biodiversity, and developing holistic analysis. However, modeling is inherently descriptive as opposed to analytically deterministic. There has been a shift away from deterministic thinking, towards a more open analysis of environmental and ecological issues. Whereas mathematics was viewed as playing an important role in ecological thought, analysis of the issues themselves has now taken centre stage and the role of mathematics somewhat subsumed. The next paradigm will see implementation of artificial neural networks (ANNs) and the use of machine learning to incorporate very large data sets and further distance modeling from constricted, analytical thinking. In some engineering applications it is the pattern itself that is the information, and the changes in the patterns reveal changes in the system. Similarly, ecology is moving toward analysis of patterns as a means of identifying change. Figure 1: Penaeid shrimp. Illustration by Jo Taylor, Museum Victoria. 2 Martin Richardson 578168 Declarations and Statements Declaration This work has not previously been accepted in substance for any degree and is not being concurrently submitted in candidature for any degree. Signed ......................................................... Date............................................... Statement 1 This dissertation is the result of my own independent work/investigation, except where otherwise stated. Other sources are acknowledged by footnotes giving explicit references. A bibliography is appended. Signed ......................................................... Date ............................................... Statement 2 I hereby give my consent for my dissertation, if relevant and accepted, to be available for photocopying and for inter-library loan, and for the title and summary to be made available to outside organizations. Signed ......................................................... Date ............................................... 3 Martin Richardson 578168 Contents Summary ....................................................................................................................................................... 2 Declarations and Statements ........................................................................................................................ 3 Acknowledgements....................................................................................................................................... 5 Abbreviations ................................................................................................................................................ 6 Abstract ......................................................................................................................................................... 7 Introduction .................................................................................................................................................. 8 Methods ...................................................................................................................................................... 16 Results ......................................................................................................................................................... 21 Discussion ................................................................................................................................................... 24 Appendix ..................................................................................................................................................... 30 References .................................................................................................................................................. 30 4 Martin Richardson 578168 Acknowledgements Data and formulae for the shrimp growth model were developed by Dr. Ingrid Lupatsch and colleagues at the Swansea University centre for sustainable aquatic research (CSAR). With thanks to Dr. Ingrid Lupatsch and Prof. Kevin Flynn for their guidance. 5 Martin Richardson 578168 Abbreviations SGR Specific growth rate DE Digestible energy DP Digestible protein DP/DE Ratio of DP to DE BW Individual body weight TGC Thermal unit growth coefficient AGR Absolute growth rate Mass balance equation IBW Initial body weight FBW Final body weight t Time D Number of days DGC Daily growth coefficient Predicted final Body Weight GE Gross energy HeE Basal metabolism HEf Fasting heat production HxE Molting energy loss MBW Metabolic body weight a Constant Metabolic rate T Water temperature RE Energy gain FCR Feed conversion ratio Feed efficiency CL Carapace length BW, CL conversion GIS Geographic Information systems 100x(lnFBW-lnIBW)/D Dependent on BW DP/DE (FBW1/3-IBW1/3))/(TxD)x100 [1] dW/dt=( C-(MR+ S)-( F+U)-G)xCal xW [2] Days 100x(FBW1/3-IBW1/3)/D (IBW1/3 +(TGC/100 x T x D))3 kJ ~ HeE 0.25xRE BWb (kg)b Related to species and T aBWb, with 0.7<b<0.9 [1] [1] [1] [1] [1] [1] [3] [4] [1] Feed/ weight gain Weight gain/feed mm Log10BW = 2.8xLog10CL -2.9 [5] 6 Martin Richardson 578168 Abstract “We left him at the seaside and returned to our ship where, in five or six hours absence, we had pestered our ship so with codfish that we threw numbers of them overboard again; and surely, I am persuaded that in the months of March, April, and May, there is upon this coast (Cape Cod) better fishing, and in as great plenty, as in Newfoundland. For the schools of mackerel, herrings, cod, and other fish that we daily saw as we went and came from shore, were wonderful...” John Brereton, 1602 Many fish stocks are now managed; wild salmon in several countries, Georges Bank cod in Canada; and fishing is regulated in many countries. Rapid oscillations and even decimation of wild stocks and a growing world population has made this a necessity. Predicting the effects of anthropogenic forcing is becoming increasingly urgent as global food security concerns develop. Some individual species are adapting and changing their range and diet, producing major modifications to ecosystems in certain instances; but overall there is a steadily increasing decline. An understanding of the ways ecosystems impact climate is needed to improve climate models. In many cases ecological studies lasting years or even decades are no longer possible as sea level rise, ocean acidification, pollution, and other factors are changing environmental conditions at an increasing rate. Ecological models offer the means to analyze these issues quickly. Simulations that cover years or even decades can be run within a few minutes or even hours providing insight pertaining to the problems in question. Accurate individual based models, such as the individual growth model discussed here do more, they provide the building blocks for assimilation into a future grand model of the entire Biosphere incorporating both climate and ecological effects. Such a model will permit improved prediction for the future and empower governmental policy making so that efforts to mitigate against climate change and other anthropogenic factors can be made as part of an overall strategy to regulate the Biosphere rather than discrete initiatives with unpredictable effects on other elements. This work utilizes the results of careful growth studies carried out by Dr. Ingrid Lupatsch at the Centre for Sustainable Aquatic Research (CSAR) at Swansea University. The model produced reproduces the equations and results of Dr. Lupatsch’s work. In particular, the primary growth equation is a simple power function that may be easily adapted to a variety of fish and shrimp species by changing the coefficient and exponent of the body weight (BW) to values associated with a particular species. It appears that the main factors affecting growth of shrimp are temperature (T) and the molting cycle. Apart from salinity, the myriad other considerations associated with growth have not been the subject of comparable studies and 7 Martin Richardson 578168 therefore do not warrant inclusion at the current time. Too detailed an approach may introduce instability and create larger errors and thereby reduce the usefulness of an individual growth model to other potential applications. Introduction “Remember that all models are wrong; the practical question is how wrong do they have to be to not be useful.” George E. P. Box There are inevitably, limitations to our ability to understand nature. Mathematics has found application to ecology, but analytical solutions to ecological problems are few and far between. It is also extremely difficult, if not impossible, to combine analytical processes so as to build complete ecosystem models. The mathematics becomes intractable. In some ways this parallels the application of models to economics and other fields such as sociology that now rely on extensive modeling to analyze problems and even predict possible outcomes[6]. The advantage of course is that modeling inherently unites all these fields. This is important in aquaculture, where profit is a fundamental driver, but it is also true of ecology and the struggle to protect biodiversity. The essential feedback from ecosystems services, the value of their production, which has been largely taken for granted. Now, the time is fast approaching when ecosystem services will be limiting factors to human population growth, and indeed, economic growth. Obviously continued growth is unsustainable, but in order to successfully divine a new economics, an incorporation of ecosystem services and environmental costs will be required. Ecology, as with many other areas of science, has sought a more rigorous foundation through the application of mathematics as well as physical and chemical laws. In-depth ecological studies are painstaking work, and can take decades to yield meaningful results. Models do not replece field research, but they can compliment or guide fields of study. Mathematical ecology has not been able to provide a global set of ecological laws. The species-area concept for example is a reductionist view of a system, and the relationship does not hold for many situations like tropical rainforests or polluted environments. Results overall have been mixed with some success in areas such as species interactions but applications involving fisheries management are frequently problematic. Solutions to management problems involve social, economic and cultural factors which are not easily modeled using mathematics. In this regard therefore, mathematics is a subdiscipline of modeling. The current 8 Martin Richardson 578168 trend is to ever more ad hoc systems and machine learning, moving away from analytical solutions and deterministic models. Here the application software selects the best mathematics, and ‘learns’ from prior analysis without human intervention. In this manner it is the patterns themselves that can be compared over time. The question has shifted to ‘what has changed’ from ‘what are the causes’. Growth has traditionally been measured using a mass balance approach[2], or in terms of absolute growth rate (AGR), specific growth rate (SGR), thermal growth coefficient (TGC), weight gain, or length. Many of these methods originated in the field of animal nutrition and their derivation is related more to system isomorphisms than rigorous analysis[7]. SGR, for example, has found extensive application despite being weight dependent and therefore not useful in comparing growth rates in animals of different weights[1]. In turn, mathematics has failed to embrace metabolic processes and falls short in providing analytical foundations for terms involving weights to a noninteger power[8]. Linear, polynomial, von Bertalanffy, Gompertz, exponential and more recently, artifical neural network models have been developed for growth[9]. Best practice[10] iincludes using multiple models, and although they are not always completely accurate, nevertheless formal models have been demonstrated to be the best means of making predictions[10]. Formal systems models are developed using application software such as PowerSim®, VenSim®, or STELLA®. These packages use a so-called systems approach which is more adaptable, easier to use and utilizes charateristics of natural systems in a similar way that analytical models are derived from system isomorphisms. The application of system dynamics means that resulting models vary considerably in their precision and character. Detailed models of of an algal population maintained in a controlled environment are mechanistic and can achieve a high degree of accuracy and produce good representation, particularly of well understood processes. It should be noted however that the model inevitably reflects the experiment within the range of empirical data. From an epistemological perspective the model forms a repository of knowledge concerning a system rather than knowledge in itself. Descriptive models using broad assumptions, and gross simplification involving a fishery over a large geographic area are necessarily less precise. But at the same time they can offer broader analysis of the commercial fishery operation, market price, employment and the like[11,12], and thereby aid policy and management decisions[13]. And, finally, individual growth models may be incorporated into a fluid dynamics model of an estuary or bay being used for aquaculture of a 9 Martin Richardson 578168 particular species. This can involve input from other commonly used software including mapping (GIS), and EcoSim[14]. Figure 2: Yu, R et al. Predicting shrimp growth. Artificial neural network versus single equation, nonlinear regression models. The artificial neural network, ANN, approach does not require specification of the numerous individual nonlinear factors in order to predict growth and results in the best growth predictions but does not illuminate the relative contribution of the many factors that affect growth. The other functions indicate the relative importance of contributing factors by the values of their coefficients. The schematic diagram below is taken from a paper by A. R. Franco et al [5], and shows a conceptual diagram of the shrimp growth model developed using PowerSim®. This model uses a von Bertalanffy equation parameterized for Panaeus indicus. It includes waste and egg production with consideration of physiological processes using formulae derived from several different sources. Originally it was developed with the intention of making it available for inclusion into broader ecological models. It was Figure 3: Franco, A R et al, 2006. Here two forcing functions are considered: food availability and water temperature. Temperature affects both the feeding rate and metabolism. 10 Martin Richardson 578168 subsequently updated to include additional factors and incorporated into a commercial software package called POND for use by shrimp farmers. It can be seen that the POND model incorporates effects due to temperature, salinity, and dissolved oxygen as well as natural food (zooplankton and bacteria) which feed on phytoplankton occurring in the water. The model also includes business planning as well as a cost and profit analysis. Figure 4: POND® shrimp aquaculture software console. An additional model derived from fish was produced by Mishra et al. in 2002[15] in attempt to try and better understand shrimp bioenergetics in order to consider fluctuations in wild populations. This model details several physiological functions especially relating to metabolism and molting. Feeding patterns are simulated (nocturnal with cessation during molting) and the discontinuous growth pattern of shrimp detailed. 11 Martin Richardson 578168 Figure 5: From Mishra et al. 2002. This model uses results from fish experiments and several assumptions when experimental data is unavailable. On a larger scale, models that integrate geographic information from GIS, and hydrodynamic models to model currents are becoming available. A good example of this is the SMILE project[14][16]. These can be used to evaluate carrying capacity of a coastal area for mariculture of shellfish by employing an individual species model; in this case it is called SHELLSIM®. 12 Martin Richardson 578168 Figure 6: Combined modelling framework used in SMILE, the Sustainable Mariculture in Irish Lough Ecosystems project. The composite framework uses 3 systems: Delft3D for hydrology, EcoWin2000 for carrying capacity, and an individual bespoke shellfish growth model, ShellSim. Results from a simple model analyzing the global shrimp market[11], which includes fishery and aquaculture production as well as environmental impacts[17] are shown below. Such a model is necessarily highly descriptive as characteristics of the global market are greatly simplified. 13 Martin Richardson 578168 Figure 7: Scenario incorporating environmental impacts of aquaculture with effluent from farms limiting production. Price rises as supply is limited. In turn the fishery grows and wild shrimp are reduced. Johnston et al. 2002. Figure 8: As wild populations are reduced in the previous scenario, wild populations are reduced, thereby increasing the cost per unit effort of the fishery. This scenario demonstrates the effect of a one-time government subsidy to commercial fisherman at month 200 leading to a brief upturn in wild catches, limitation of aquaculture production and steady increase in price. Johnston et al. 2002. Key to model outputs Actual price Global shrimp price 14 Martin Richardson 578168 Harvesting Aquaculture production Catching Fishery production Wild shrimp biomass Production of P. Vannamei has undergone a complete shift from wild fishery to aquaculture starting around the turn of the millennium[18], due in part to development of disease resistant strains[19]. One scenario considered using the model, termed ‘Unbounded Aquaculture’ was closest to the mark but was not considered a likely outcome. Illustrating how bias can influence the use of a model. Analysis was skewed towards maintenance of a commercial fishery. Shrimp characteristics Penaid shrimp are marine but may spend part of their lifecycle in brackish estuaries[20]. Shrimp live for 1.5 to 6 years with multiple cohorts in wild populations. In aquaculture there may be more than one cohort in a pond. As with all crustaceans growth is discontinuous with periods of rapid growth, no growth, and even negative growth particularly related to water temperature[21]. Growth can be measured in terms of carapace length, total length or weight. The molt cycle is dependent on the species in question as well as the age and size of the individual animal. Wild populations synchronize molting with lunar cycles and bury themselves in the substrate to minimize predation and cannibalism while the carapace hardens. The molting interval is affected by food availability and environmental conditions, particular temperature[22]. Molting duration increases as the temperature drops. Water is taken up during molting, with rapid gain in the wet weight of replacement exuvia. However, the majority of somatic growth occurs during intermolt periods. Feeding is nocturnal in many species, and there is a break of several days in feeding while molting occurs. Feed intake increases with temperature. Light sensitivity to feeding varies widely, with some species only active for 1 to 2 hours a night[23]. Shrimp show sexual dimorphism with females being larger, and growing faster than males. Females achieve maturity at approximately 30-40 mm, after some 6 months, and produce from 100,000 to over 250,000 eggs[23][24]. They usually spawn twice annually, although some species spawn only once each year. Survivability is related to both temperature and salinity with increased salinity mitigating against critical low thermal limit associated with mortality. Mortality due to cannibalism, particularly during molting, is a factor in aquaculture. Aquaculture studies have shown significant 15 Martin Richardson 578168 increased production potential through the use of a ‘substrate’ made of plastic mesh that permits vertical separation of the shrimp[25], thereby greatly reducing the effective stocking density. Methods Shrimp bioenergetics research by Ingrid Lupatsch et al. at Swansea University’s’ centre for sustainable aquatic research (CSAR) assessed protein and energy requirements for optimal growth of shrimp under controlled conditions. Knowing daily protein and energy requirements for provides for improved aquaculture practice in reducing waste and optimizing feed. Waste from aquaculture facilities is an important problem[17] and results in contributions to dissolved organic matter as well as benthic deposits[26]. Feed may account for as much as 50%[27] of all costs. Various protein and energy sources can be used to formulate a feed to known composition. Until quite recently feed composition in aquaculture was determined by comparison with dietary requirements of domesticated animals (e.g. chickens). Since shrimp aquaculture is now a large industrial enterprise around the World such calculations are of economic importance. For pacific white shrimp (L. vanneamei) growth was measured by increase in wet weight, the body weight (BW) in grammes at 28°C with 32 ppt (parts per thousand) water salinity. pH and dissolved oxygen (DO) were not specified. The equation for growth rate applies to post larval juvenile and adult stages over the range of 1 g to 35 g. This is an individual growth model so all quantities are per shrimp. Growth rate is measured as incremental body weight per day, weight gain (g day-1). = 0.050 × BW 0.582 , for 1g ≤ BW ≤35g (1) Growth rate (g day-1) (2) Voluntary feed intake rate (g day-1) = 0.086 × BW 0.720 Energy requirements in kJ per day Digestible energy required for maintenance, DEmaint (kJ day-1) DEmaint=0.345xBW (kJ day-1) (3) (4) Expected energy gain (kJ day-1) =(growth rate)x(energy content of gain) Energy content of gain =4.844 kJ g-1 Energy gain = 0.050 × BW 0.582x4.844 (kJ day-1) Digestible energy requirement for growth, DEgrowth (kJ day-1) (5) DEgrowth =(growth rate)x3.23x(cost in DE to deposit a unit of energy as growth) 16 Martin Richardson 578168 DEgrowth =0.050 × BW 0.582x3.23 x4.844 (kJ day-1) (6) Digestible energy for maintenance and growth DEmaint+growth= DEmaint+DEgrowth (kJ day-1) Protein requirements in grammes per day = 0.75xBW(g day-1) (7) Digestible protein required for maintenance (DPmaint) (8) Expected protein gain Protein content of gain =(growth rate)x(protein content of gain) = 17.2% Protein gain = 0.050 × BW 0.582x0.172 (g day-1) (9) Digestible protein required for growth (DPgrowth) = (protein gain)x(cost in DP to deposit a unit of protein as growth) DPgrowth = 0.050 × BW 0.582x0.172x2.270 (10)Total DP required for maintenance and growth (DPmaint+growth) = DPmaint+DPgrowth Feed related calculations Let DE content of feed = 14 kJ g-1 .Then the … (11)required feed intake to meet DE requirements (g) = DEmaint+growth/(DE content of feed) =DEmaint+growth/14 (12)Required DP content of feed to meet DP requirement (%)=(DPmaint+growthx100)/Feed intake =(DPmaint+growthx100)/(DEmaint+growth/14) =14x100xDPmaint+growth/DEmaint+growth =1400xDPmaint+growth/ DEmaint+growth (13)Feed conversion ratio (FCR) =(feed intake)/(weight gain) (14)DP/DE ratio =DP content of feed (mg)/DE content of feed(kJ) 17 Martin Richardson 578168 Energy Energy content of body Growth Rate Energy gain Total energy component DEefficiency DEgrowth DEmaint DEcost Body Weight DEmaint&growth Figure 9: Energy component of model. The diamonds are constants. Circles and oblongs are variable quantities that are calculated using inputs from incoming arrows. The bracketed variables reference the same quantities at different locations within the model without using connecting arrows and thereby reduce clutter. Model object name Quantity Value/ Equation number Energy content of body Constant 4.844 kJ g-1[28] Total energy component (Energy content of body)x(body weight) DEmaint DEmaint (3) DEgrowth DEgrowth (5) DEmaint&growth DEmaint+growth (6) Energy gain (4) DEefficiency Constant 0.31 DEcost 1/DEefficiency 3.23 18 Martin Richardson 578168 Protein protein content of body Growth Rate Protein gain Total protein component DPefficiency DPmaint DPgrowth DPcost Body Weight DPmaint&growth Figure 10: Protein related calculations of the model. Model object name Quantity Value/ Equation number Protein content of body Constant 0.172 [28] Total protein component (protein content of body)x(body weight) DPmaint DPmaint (7) DPgrowth DPgrowth (9) DPmaint&growth DPmaint+growth (10) Protein gain (8) DPefficiency Constant 0.44 DPcost 1/DPefficiency 2.27 19 Martin Richardson 578168 Body Weight Growth Rate b a Voluntary Feed Intake Figure 11: PowerSim model of energy, protein and feed calculations applied to growth of L. Vannamei Model object name Quantity Value/ Equation number a Coefficient of MBW 0.05 b Exponent of BW 0.582 Growth rate Body weight (1) BW g Voluntary feed intake (2) 20 Martin Richardson 578168 Results Allometric weight gain Weight gain (g per day) 0.4 0.3 0.2 0.1 0 5 10 15 20 Body weight (g) Non-commercial use only! Feed intake (g per day) Feed intake vs. body weight 1.0 0.5 0 5 10 15 20 Body weight (g) Non-commercial use only! Figure 12: Model results for allometric weight gain and feed intake against body weight. These results match the calculations given in Lupatsch et al. 2008 as listed below. 21 Martin Richardson 578168 Figure 13: Lupatsch et al. 2008. Studies on Energy and Protein Requirements to Improve Feed Management of the Pacific White Shrimp, Litopenaeus vannamei. The calculations have been completed for shrimp at 2 different weights: 2g and 10g. Feed calculations follow from specified digestible energy (DE) content which was set at 14 kJ g -1. 22 Martin Richardson 578168 Growth in L. vannamei at 28 C with 32 ppt salinity and optimal feeding Body Weight (g) 30 20 10 0 0 20 40 60 80 100 120 140 Time in days Non-commercial use only! Figure 14: Early stage growth. The curve has not yet reached the asymptotic weight as the equation is only valid for shrimp below 35g. Lupatsh et al. 2008. Feed DEmaint&growth Required feed intake rate FCR DPmaint&growth DEcontent Growth Rate DPcontent DP DE ratio Figure 15: Feed calculations Model object name Quantity Value/ Equation number DEmaint&growth DEmaint+growth (5) 23 Martin Richardson 578168 DPmaint&growth DPmaint+growth (9) Required feed intake rate Required feed intake for growth (10) DEcontent DE content of feed (constant) 14 kJ g-1 DPcontent Required DP content of feed (11) (%) DP DE ratio DP/DE (13) FCR Feed conversion ratio (FCR) (12) Required feed intake rate Voluntary feed intake Feed intake in grammes 1.0 0.5 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 Time in days Non-commercial use only! Figure 16: Model results showing voluntary feed intake against actual feed intake. Discussion Experimental results for shrimp growth, as well as growth models[5,15,29] all show generally monotonic, asymptotic curves from juvenile through to adult stage. While there are numerous factors that affect growth at the individual level, temperature and the molting cycle appear to be most important. Growth has also been found to be affected by stocking density and biomass as well 24 Martin Richardson 578168 as water salinity, pH, naturally occurring food[30][31] and photoperiod. There are numerous studies in the literature addressing one or more of these environmental factors; however no clear picture emerges as to how they interact to affect growth in combination. The mode developed by Franco et al. [5] indicates sensitivity to temperature as shown below and this is also evident from experiments and aquaculture. Figure 17: Franco, A. R. et al, 2006. The model developed by Franco et al. is not sensitive to a ±10% variation in food availability, but shows susceptibility to a ±10% change in water temperature indicating that temperature has a major effect on growth. Temperature considerations Wild shrimp populations and pond shrimp are subjected to diurnal and seasonal temperature variation. The characteristics of these temperature changes are well understood[32]. Seasonal temperature effects upon growth can be added to the model. Temperature curves are generally sinusoidal with a winter minimum and summer maximum as shown below. 25 Martin Richardson 578168 Figure 18: Adamack et al. 2012. Seasonal temperature curve for wild shrimp population. Figure 19: Weekly pond water temperature in Australian aquaculture. Seasonal variation is clearly evident. The shaded area shows weekly maxima and minima. Jackson, C. J. et al. 1998. These temperature changes can then be combined with the variation in growth at different temperature in order to model the effect. The expected results are accelerating or decelerating 26 Martin Richardson 578168 growth with periodic negative growth dependent upon response for the species in question and environmental temperature range. Figure 20: Growth in length at various temperatures. Species not identified. Source: Northern Gulf Institute, data derived from the literature. Growth ceases at 0°C and increases to a maximum rate at around 25°C. As temperature increases, growth declines and stops at approximately 35°C. Continuing temperature increases result in mortality. This is a problem in shallow aquaculture ponds in the tropics. Low temperatures can decimate overwintering stocks in wild shrimp. Food intake and growth increase with water temperature but decline beyond the optimal point. Franco et al. [5] used a quadratic equation as a temperature function ,𝑓(𝑇), to model the effect of temperature on shrimp feeding rate. (15)𝑓(𝑇) = −0.02𝑇 2 +1.44𝑇−17.41 𝑅32 [5] Where R32 is the maximum feeding rate at the optimum temperature of 32°C for P. japonicus. If we assume that the behavior is similar for P. vannemei, and that the maximum feeding rate is achieved at, say 27°C then the general form of the graph of this function is as follows: 27 Martin Richardson 578168 Figure 21: General form of the temperature function with temperature in °C on the x-axis. The y-axis value represents a growth coefficient, as yet not defined. Using the graph in figure (18) above taken from Adamack et al. 2012 , the annual temperature variation in this location (Mississippi, USA) is approximately 22°C over 365 days (1 year). We can approximate this without considering the actual date using the following equation: (16)Water temperature=11sin( 360𝐷 /365) + 24, where D is the time in days. Water temperature 40 30 20 10 0 0 20 40 60 80 100 120 140 Time in days Non-commercial use only! Figure 22: Natural fluctuation in water temperature associated with seasonal change over time. Equation (16) can then be adjusted to match particular dates as required. Further studies, possibly combined with salinity variation[33], are needed in order to better understand temperature effect. A study by Rothlisberg, 1998 [34]considered larval growth as illustrated below 28 Martin Richardson 578168 Figure 23: Rothlisberg, 1998. Effects of salinity and temperature on growth of shrimp protozoae. Molting Figure 24: Simulated oxygen consumption during 3 stages of moulting, pre-moult, moult, and post-moult. Mishra et al 2002. In actuality the cycle increases with size and age of the individual. P. vannamei, molt every 5 days at 1-5g and every 14 days at 15g[23][35]. Information regarding the molting cycle permits derivation of a scalar quantity that can be used to stretch the timeline, thereby increasing both the inter-moult period and the length of the molt itself to better match observed behavior. However, molting is also affected by temperature as shown below. Molting interval increases inversely with temperature[36][22], tripling as temperature drops from 18°C to 14°C. 29 Martin Richardson 578168 Figure 25: Molting period at 3 different temperatures. Kumlu et al 2005. Conclusion In order to improve the model additional experiments are needed to better understand the effects of salinity and temperature, molting and spawning. There is little available data in the literature concerning shrimp bioenergetics in general and environmental effects in particular. There is need to standardize nomenclature, notation and formulae so as to provide a more rigorous foundation for this field of research and to avoid errors of interpretation. A standardized metadata and verification algorithm would also permit easier combination and exchange of models. The primary value is in the data, as well as associated costs so best practice might involve the sharing of ‘open’ models through the use of an industry ‘ecosystem’ of stakeholders. This would ensure improved scrutiny of the methods used and enable the facilitation of popularity ratings and error calculation to indicate stability as an indication of descriptive vs. quantitative purpose. Appendix Body weight to carapace length conversion A body weight to carapace length conversion formula[5] was developed by Franco et al. in their paper ‘Development of a growth model for penaeid shrimp’. Shrimp were measured and weighed to determine the relationship. The species used was Penaeus indicus. Log10BW = 2.8xLog10CL -2.9 References 30 Martin Richardson 578168 1 Bureau, D. & Azevedo, P. 2000 Pattern and cost of growth and nutrient deposition in fish and shrimp: Potential implications and applications. , 111-140. 2 Adamack, A., Stow, C., Mason, D., Rozas, L. & Minello, T. 2012 Predicting the effects of freshwater diversions on juvenile brown shrimp growth and production: a Bayesianbased approach. Marine Ecology Progress Series 444, 155-173. (doi:10.3354/meps09431) 3 Lupatsch, I. 2009 Quantifying nutritional requirements in aquaculture: the factorial approach. New Technologies in Aquaculture. Woodhead Publishing Limited, Cambridge, UK , 417–439. 4 Lupatsch, I. In press. Aquatic Animal Nutrition Feed formulation. Growth (Lakeland) 5 Franco, a, Ferreira, J. & Nobre, a 2006 Development of a growth model for penaeid shrimp. Aquaculture 259, 268-277. (doi:10.1016/j.aquaculture.2006.05.051) 6 Daviddottir, B. 2002 Management of the Commons: Social Behavior and Resource Extraction. 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