Pathophysiology of Inflammation Miklós Molnár Essentials of Inflammation Inflammation is a complex defense reaction in the vascularized connective tissue in response to endogenous or exogenous stimuli in order to destroy, eliminate the injurious agents or microorganisms and initiate a series of events that heal and reconstitute the damaged tissue Subsequent Events Alteration (tissue injury) Vascular reaction Proliferation (reconstitution) Localization The connective tissue and its microcirculation including the surrounding extracellular space. Participants Plasmaproteins – Proteolytic cascades: coagulation, kinin, fibrinolytic Blood cells – Neutrophils, eosinophils, basophils, monocytes, lymphocytes, platelets Cells of the connective tissue – Mast cells, fibroblasts, macrophags, lymphocytes Blood vesels – Endothelium, basement membrane, smooth muscle cells Participants Extracellular matrix – Fibronectin, laminin, collagen, enactin, tenascin, proteoglikans etc. Specific mediators – Early mediators positive, amplification – Late mediators negative, restitutio Injury Acute Inflammation or Restitution Chronic Inflammation or Restitution Proliferation (granuloma) Restitution Types of Inflammatory reactions Type Acute Inflammation Duration max. 1 week Characteristics exudation (edema) emigration of leukocytes Chronic Inflammation > 1 week connective tissue proliferation lymphocytes and macrophages accumulation Inflammation Local reactions Systemic reactions Classical (cardinal) Acute phase reaction, fever, leukocytosis etc. signs Celsus described the four famous signs of inflammation calor, rubor, tumor, and dolor(A.D. 30) Systemic Effects of Inflammation Acute-phase reaction TNFa, IL-1 & IL-6 fever leukocytosis Iron deficiency, anemia proteolysis activation of lymphocytes g-INTERFERON IMMUNCOMPLEX NEUROPEPTIDES MACROPHAGES ENDOTOXIN EXOTOXIN Initiation of Acute Phase IL-1 IL-6 Reaction URATE SILICATE CRISTALS TNFα Acute Phase proteins Ceruloplasmin – Scavenges oxygen radicals generated by leukocytes Protease inhibitors – α1-protease inhibitor, α1-antichymotrypsin and α2-macroglobulin C-reactive protein (CRP) – binds to bacteria and produce capsular swelling, precipitation and agglutination; binding also fixes complement, thus causing the production of C3b (an opsonin) and chemotactic factors Fibrinogen – may serve as opsonin by clumping bacteria together; breakdown products of fibrinogen has anti-inflammatory activity Transferrin – decreases, thus limiting the amount of iron available to meet bacterial growth requirement P A T H O M E C H A N I S M O F INFECTION, TOXINS, IMMUNCOMPLEXES, NEOPLASIA IL-1/TNF Hypothalamus F E V E R Prostaglandins (E2) Vasomotor center ? Sympathetic nerves Skin vasoconstriction ↓ Heat dissipation FEVER IL-6 Local Effect of Acute Inflammation Blood flow increases (alteration of vascular caliber) Increased vascular permeability (plasma proteins and leukocytes leave the circulation, retraction of endothelial cells, fenestration) Cellular events (emigration of leukocytes and makrophages: margination, rolling, adhesion, emigration, chemotaxis, phagocytosis) Advantages of exudation Fluid exudation (dilution of toxins) Increased protein content Globulins (antibodies) Fibrin precipitation (Bacterial fixation, wound healing) Acute-phase proteins Mechanisms of Vascular Leakage 1. Endothelial contraction widening of intercellular junctions (rapid , short-lived action, histamine, bradykinin, leukotrienes etc. effects only the small venules) 2. Junctional retraction induced by IL-1, TNFα, IFNg, delayed (4-6 hours) effect 3. Leukocyte-dependent leakage free radicals and proteolytic enzymes 4. Leakage from regenerating capillaries angiogenesis, intercellular junction development Leukocyte extravasation Margination, rolling and adhesion Transmigration across the endothelium (diapedesis) Migration in interstitial tissues toward a chemotactic stimulus Adhesion receptors Selectins – E-selectin (ELAM-1, endothelium) – P-selectin (GMP140, platelets) – L-selectin (LAM-1, leukocytes) Immunoglobulins endothelial adhesion molecules: intercellular (ICAM-1), vascular cell ~ (VCAM-1) both interact with integrins found on leukocytes Integrins (transmembrane-adhesive heterodimeric glycoproteins, α and β chains) 2-integrins (LFA-1, MAC-1 ICAM-1) 1-integrin (VLA-4 VCAM-1) Cell Surface Adhesion Receptors Roles and Mechanism of Adhesion Molecules 1. Redistribution of adhesion molecules to the cell surface P-selectin (intracitoplasmatic granules of endothelial cells - Weibel-Palabe granules histamin, thrombin, PAF cell surface adhesion and rolling of leukocytes Roles and Mechanism of Adhesion Molecules 2. Induction of adhesion molecules on endothelium (IL-1, TNF) E-selectin, and ICAM-1, VCAM-1 3. Increased avidity of binding LFA-1 on leukocytes dose not adhere to its ligand ICAM1 on endothelium at resting condition. However, after certain stimuli LFA-1 is converted from a state of low- to high-affinity binding toward ICAM-1 Strong binding Transmigration across endothelium Migration of Leukocytes Selectins Integrins Chemotaxis and Leukocyte Activation Exogenous substances – bacterial products (N-formyl-methionine containing peptides, lipids) Endogenous substances Compounds of complement system ( C5a, C3a) Products of the Lipoxygenase pathway (LTB4) Cytokines (IL-8) receptor bindings PLC-Ca2+ actin-myosin active locomotion and activation of PLA2 AA, degranulation (lysosomal enzymes), modulation of adhesion molecules Chemotaxis Phagocytosis 1. Recognition and attachment – opsonins mediated (Fc-fragment of IgG, C3b) (via FcgR receptors) – nonopsonic phagocytosis recognition of LPS Phagocytosis 2. Engulfment Binding to FcgR pseudopods fusion of the phagocytic vacules and the lysosomal granules Killing or Degradation Oxygen dependent: NADPH oxidase superoxide ion H2O2 MPO H2O2 + Cl- HOCl NO synthase NO peroxinitrite (CONO2- ) Oxygen independent: lysozyme, lactoferrin, MBP –major basic protein-, defensins, acid hydrolase Phagocytosis Defects in Leukocyte Functiom Genetic Disease Defect Leukocyte adhesion deficiency 1 β chain of CD11/ CD18 integrins Leukocyte adhesion deficiency 2 Selectin receptors (Sialyated oligosacharide) Neutrophil specific granule deficiency Absence of neutrophil specific granules, defective chemotaxis Chronic granulomatosus disease Decreased oxidative burst – X-linked – NADPH oxidase (membrane component) – Autosomal recessive – NADPH oxidase (cytoplasmic component) Myeloperoxidase deficiency Absent MPO-H2O2 system Chédiak-Higashi syndrome Multiple defect Defects in Leukocyte Functiom Acquired Disease Thermal injury, diabetes, malignancy, sepsis, immunodeficiencies Hemodialysis, diabetes Leukemia, anemia, sepsis, diabetes, neonates, malnutrition Defect Chemotaxis Adhesion Phagocytosis and microbicidal activity Plasma Proteases Kinin system Fibrinolytic system Complement system The Kinin system Bradykinin 1. Inducing arteriolar dilatation 2. Increasing the permeability of venules 3. Causing pain Kallinkrein 1. Activation of plasminogen 2. Activation of C1q in the complement system 3. Found in plasma , tissues and secretions The Fibrinolytic System Plasminogen Activating Factors prourokinase urokinase Kallikrein Plasmin 1. Produce vasodilatation by generating FDP 2. Can digest fibrin, removes fibrin deposit 3. Can activate complement system by cleaving C3 Other Plasminogen Activators Bacterial substances (eg. streptokinase) Cell-derived activators (eg. trypsin) Macrophage plasminogen activator Hageman factor Note: Plasmin was formerly known as fibrinolysin, this old name is still used for this system The Complement System Consist of 20 interactive plasma and cell membrane proteins Activated complement: – – – – Mediating vascular responses (histamine release) Requiting phagocytic leukocytes (chemotaxis) Opsonizing target of phagocytic cells Directly damaging target cells or tissue Activation: – Classic pathway – Alternative pathway Classic Pathway This pathway is activated by antibody-coated targets or antigenantibody complexes Hageman Factor C1 inhibitor Alternative Pathway Activated by: 1. LPS; 2. plasmin; 3. a factor from cobra venom; 4. aggregated IgM or IgG; 5. complexes of antigen with IgA C3b inhibitor The Role of Hageman Factor C1 inhibitor Chemical Mediators of Inflammation Vasodilatation Prostaglandins, histamin Vascular permeability Vasoactiv amins C3a and C5a Bradykinin Leukotrien C4, D4, E4, PAF Chemotaxis C5a, Leukotrien B4 , lipids, bacterial products Chemical Mediators of Inflammation Fever IL-1; TNF, Prosztaglandin E2 Pain Prosztaglandins, Bradykinin Tissue injury Lysosomal enzymes (neutrophils, macrophages) oxygen metabolites Cyclo-oxygenase Hypothesis - 1990’s membrane phospholipids (-) Phospholipase A2 glucocorticoids (-) arachidonic acid COX-1 constitutive (-) Classical NSAIDS COX-2 (-) Stomach Kidney Endothelium PGE2/PGI2 PGE2/PGI2 PGI2 gastric cytoprotection renal blood flow inducible Platelets TXA2 haemostasis Physiological Effects INDUCTION: mitogens endotoxins cytokines (-) selective COX-2 inhibitors Inflammatory Sites PGE2 inflammatory mediators Inflammation Hierarchies of cytokines Stimulus TNF a IL-1 IL-6, IL-8, VEGF, GM-CSF Effect of anti-TNFa Treatment Decreased synthesis of IL-1, IL-6 Inhibition of the migration of leukocytes Decreased number of endothelial adhesion molecules (VCAM-1, ICAM-1, E-selectin) Decreased endothelial chemotactic chemokins (IL-8) Decreased T-cell infiltration (T CD4 sup+, CD45 RO sup+) Chronic Inflammation Inflammation of prolonged duration (weeks, months) in which active inflammation, tissue destruction and attempts to healing are proceeding simultaneously – – – – infiltration with mononuclear cells tissue destruction attempt to repair by connective tissue (fibrosis) angiogenesis Causes of Chronic Inflammation Persistent infection Prolonged exposure to toxic agents Immune reactions against own tissue autoimmune disease (SLE, RA)