What is the difference between an autotroph and a heterotroph? Why do plants need sunlight? What gases are exchanged between plants and animals? To be able to list the inputs and outputs of photosynthesis. To be able to identify the structures in a chloroplast. To be able to summarize light-dependent reactions. Light energy ------> chemical energy Used by autotrophs to produce food All of our energy starts as light energy! Plants use sunlight to make food animals eat plants other animals those animals Go through Photosynthesis to produce GLUCOSE… Autotrophs “Producers” in the food web Consist of plants, protists, cyanobacteria 1) Light dependent Light energy is absorbed and converted into chemical energy in the form of ATP and NADPH. (also produces Oxygen!) 2) Light Independent (Calvin Cycle) CO2 ATP and NADPH are used to make glucose. *Glucose is the basic building block for more complex sugars such as starch.* Glucose Thylakoids: flattened sac-like membranes arranged in stacks (stacks are called grana). Light-dependent reactions take place here. Electron transport occurs in the thylakoid membrane Stroma: Fluid filled space outside the grana. Light-independent reactions take place here. Step 1: Light energy reaches photosytem II causing the water molecule to split H H O H 2O H+ + O2 *H+ is released to the electron transport system. * O2 is given off as a byproduct. Step 2: Light energy reaches photosystem I and electrons flow from photosystem II to photosystem I. (to replace the electrons lost) Step 3: Hydrogen ions (protons) are pumped across the membrane as the electrons fall. (think of a hyper child losing energy) Step 4: Electrons from photosystem I move to a protein called ferrodoxin. **Ferrodoxin = “helper” protein** Step 5: Ferrodoxin transfers electrons to NADP+ forming NADPH. (We need this for the next part of photosynthesis!) Step 6: Hydrogen ions move through the ATP Synthase because of the concentration gradient, creating ATP. (Chemiosmosis) To Calvin Cycle Lets see it altogether now! http://www.science.smith.edu/departments/Bi ology/Bio231/ltrxn.html What goes into a light dependent reaction? What is the goal of a light dependent reaction? What is given off as a byproduct? What is the name of the protein needed in a light dependent reaction? In what part of the chloroplast do light reactions occur? What are the outputs of light-dependent reactions? What is the name of the cycle in lightindependent reactions? List the inputs and outputs of the Calvin Cycle Define chemiosmosis and it’s role in photosynthesis. Summarize light – independent reactions Perform chromatography to identify the pigments of green leaves. Takes place in the stroma Uses NADPH and ATP from Phase I (light dependent reactions) Needs CO2!!! Produces GLUCOSE Functions like a sugar factory within a chloroplast **Regenerates the starting material with each turn Inputs - ATP NADPH CO2 Outputs - Glucose Mechanism in which ATP is produced as a result from the flow of electrons down a concentration gradient. Ex: Light reactions (H+ ions and ATP synthase) Light and Water can limit the amount of photosynthesis a plant can perform. Some plants develop alternate pathways to maximize energy conservation. Ex: CAM and C4 plants. http://www.science.smith.edu/departments/ Biology/Bio231/calvin.html