Foundations of Research Introductory lectures 2: The Role and Structure of Science What does science do? The core features of a research study. Overall Research approaches. © Dr. David J. McKirnan, 2015 The University of Illinois Chicago McKirnanUIC@gmail.com Do not use or reproduce without permission 1 Foundations of Research Introductory lectures 2: The Role and Structure of Science What does science do? The core features of a research study. Overall Research approaches. 2 Foundations of Research What does science do? Section Overview What does Science do? 1. Describe the world 2. Made predictions (hypotheses) 3. Develop & test theories 4. Apply findings or theories 3 Foundations of Research 1. Describing the world 1. Qualitative Description Simple observation: what goes on “in the wild”. Anthropology, sociology, ethnography, ethology…. Many quantitative studies begin with qualitative observations. Typically structured: field notes, checklists, behavioral counts... 4 Foundations of Research Describing the world 1. Qualitative Description Focus groups, structured observations… … valuable for formulating hypotheses. 1. 2. 3. 4. Description5 Prediction Theory Application Foundations of Research Science and description 1. 2. 3. 4. Description6 Prediction Theory Application Description … key building block in all areas… World Music: A Retrospect Across The Globe, Venus Umesh. Link. Mathematics Describing the Real World: Pre-calculus and Trigonometry. Bruce H. Edwards, link here. Foundations of Research 1. 2. 3. 4. Describing the world Description7 Prediction Theory Application 2. Quantitative Description Tracking behavior or systems using numbers Surveys, polls… Archival data - uniform crime reports. Physical observations Useful for both developing and testing hypotheses. From: Climate Site; How do climate models work? Link. Examples of Descriptive Research Foundations of Research 1. 2. 3. 4. Taxonomies: Classification systems Paleontology; shared v. distinct evolutionary characteristics Psychology, Psychiatry; behavioral patterns or disorders. Demographics: ethnicity, socio-economic status, language group… Description8 Prediction Theory Application Foundations of Research Examples of Descriptive Research 1. 2. 3. 4. Description9 Prediction Theory Application Epidemiology; Disease / behavior patterns in a population. Where is AIDS most common in the U.S.? Distribution of gun assaults, by state. Methods: Direct measurement (e.g., surveys, disease reporting) Standard records (e.g., medical visits & diagnoses) http://filipspagnoli.wordpress.com/2011/11/17/human-rights-maps160-gun-crime-in-the-u-s-in-2010/ Archival data (e.g., auto accident reports). Foundations of Research Descriptive research 1. 2. 3. 4. Description10 Prediction Theory Application EXAMPLE Paleontology attempts to accurately describe the predecessors of humans to understand evolution Carefully describing specimens and the conditions where they are found can: Produce insights into environmental change and evolution Allow us to test hypotheses & develop theory. Foundations of Research 2. Science and Predictions Practical applications: 11 What test score best predicts college success? Can I predict which employees will steal? These applications (should) stem from a theory. Verbal & quantitative aptitude academic success A specific personality constellation criminality… That is (or should be…) supported by empirical evidence. Foundations of Research Science and Predictions Practical applications: Testing hypotheses: 1. 2. 3. 4. Description12 Prediction Theory Application An hypothesis is a prediction Correlational research; I predict: cultures undergoing rapid economic change will be more prone to irrational beliefs. Experimental research; Lab: make Experimental group socially uncertain. Control: simple distraction. I predict: experimental group more prone toward superstitious beliefs. Foundations of Research 3. Developing & Testing Theory Theory is the ‘bottom line’ of science Identify basic (social, Ψ, physical…) processes… that are systematically related… that show how or why something works. i.e., what “causes” a phenomenon in the natural world. 13 EXAMPLE Foundations of Research Predictions and theory development New data have led us to rethink basic theories of human evolution. From a simple progression… …to a widening “bush” of parallel species. 1. 2. 3. 4. Description14 Prediction Theory Application EXAMPLE Foundations of Research Predictions and theory development As paleontologists rethink the shape of the evolutionary tree.. They reconsider the basic processes that shape human evolution. 1. 2. 3. 4. Description15 Prediction Theory Application Foundations of Research 1. 2. 3. 4. 3. Testing theory: Mediating effects Description16 Prediction Theory Application We often use theory to explain an observation or correlation. What might lead to superstitions or irrational beliefs? A. We describe a simple empirical effect (correlation): Social & economic uncertainty Irrational beliefs Foundations of Research 3. Testing theory: Mediating effects 1. 2. 3. 4. Description17 Prediction Theory Application What might lead to irrational beliefs? A. We describe a simple empirical effect (correlation): B. How does this work? What Theory(ies) may explain this? Uncertainty ✓ Need to feel control Irrational beliefs ✓ Lessened Critical thinking Theory = “Mediating Variables” that explain the effect of uncertainty on beliefs. Foundations of Research 1. 2. 3. 4. Theory and process Uncertainty Need to feel control Lessened Critical thinking Theory: ...identifies basic economic / psychological processes; …specifies how they may be related; …generates testable hypotheses. Description18 Prediction Theory Application Irrational beliefs Foundations of Research 4. Applications of theory Use theory to design interventions Educational theory: Design of this course Being active Cognitive “chunking” Multi-media attention 1. 2. 3. 4. …an intervention study can test a theory Behavioral or biomedical interventions compare treatments based on differing theories. Description19 Prediction Theory Application EXAMPLE Foundations of Research Theories and interventions 1. 2. 3. 4. Smoking & social networks: people are influenced by their friends’ smoking Intervention study of smoking cessation: quitting with friends may be more effective. Description20 Prediction Theory Application Foundations of Research Theories and interventions EXAMPLE Theory: social networks smoking. Hypothesis: networkbased program would work better. Testing the hypothesis: Pointed to a practical intervention approach; Supported the theory. 1. 2. 3. 4. Description21 Prediction Theory Application Foundations of Research The values of science & empiricism Empirical description Qualitative vs. quantitative… Developing hypotheses. SUMMARY Predictions Core feature of a hypothesis; Force us to clearly test our ideas. Developing and testing theory Central to science; explain how a natural process works. Applications of theories Expand and elaborate a theory. Advances in, e.g., technology, behavioral science 22 Foundations of Research Elements of science, review 1 Epidemiology is… A. A way of classifying people or behaviors B. The study of how frequent a behavior or “condition” is across a population. C. The identification of basic psychological principles that underlie important behaviors D. A core feature of a hypothesis. 23 Foundations of Research Elements of science, review 2 A Theory is… A. A way of classifying people or behaviors B. The study of how frequent a behavior or “condition” is across a population. C. The identification of basic psychological principles that underlie important behaviors D. A core feature of a hypothesis. 24 Foundations of Research Elements of science, review 3 A Prediction is… A. A way of classifying people or behaviors B. The study of how frequent a behavior or “condition” is across a population. C. The identification of basic psychological principles that underlie important behaviors D. A core feature of a hypothesis. 25 Foundations of Research Elements of science, review 4 A Taxonomy is… A. A way of classifying people or behaviors B. The study of how frequent a behavior or “condition” is across a population. C. The identification of basic psychological principles that underlie important behaviors D. A core feature of a hypothesis. 26 Foundations of Research Introduction to science, 5 What does science do? Core features of a research study. Overall Research approaches. 27 Foundations of Research 28 The overall “flow” of research Phenomenon - Larger question the research addresses Section Overview Theory - Explanatory processes & how they are related Hypothesis - Concrete variables -Specific prediction Methods / Data - Operational definitions - Study procedures Results - Hypothesis-wise analysis of outcomes Discussion & Conclusion - Relate results back to theory - Study limitations & Future studies Foundations of Research 29 Research Flow Phenomenon - Larger question the research addresses What needs explaining? Why is it important? Theory How / why do I think it works? - Explanatory processes & how they are related Hypothesis - Concrete variables -Specific prediction What concrete evidence or data will I collect? What is my specific prediction? Methods / Data - Operational definitions - Study procedures Results - Hypothesis-wise analysis of outcomes What do the results mean for the theory? What is unanswered? Discussion & Conclusion - Relate results back to theory - Study limitations & Future studies What was the outcome? Hypothesis supported? Foundations of Research 30 Research Flow The research flow begins with broad, abstract questions Phenomenon - Larger question the research addresses Theory - Explanatory processes & how they are related Hypothesis - Concrete variables -Specific prediction Methods / Data - Operational definitions - Study procedures Results Then back to a more general discussion. - Hypothesis-wise analysis of outcomes Discussion & Conclusion - Relate results back to theory - Study limitations & Future studies Gets more specific & concrete Foundations of Research Core features of research: Theory. Phenomenon - Larger question the research addresses Theory - Explanatory processes & how they are related Hypothesis - Concrete variables -Specific prediction Theory: Methods / Data - Operational definitions Abstract statement of how basic processes relate to each other… - Study procedures Results …how or why the phenomenon - Hypothesis-wise analysis of“works.” outcomes Discussion & Conclusion - Relate results back to theory - Study limitations & Future studies 31 Foundations of Research 32 Theory Basic physical or behavioral processes are the building blocks of theories. Economic uncertainty Cognitive style Stress Gravity Learning… Foundations of Research 33 Theory They are abstract: Economic uncertainty We cannot actually “see” Cognitive style gravity. We just see what it does (stuff falls). Stress Gravity Learning… Foundations of Research 34 Theory Gravity is a Hypothetical Construct: Economic uncertainty Central to our Cognitive style understanding of nature. Abstract; we cannot measure it directly. We measure and understand it by observing its effects on the world. Stress Gravity Learning… Foundations of Research Theory 35 Foundations of Research How do we use theory in research? Test a theory: Do women who feel strong stereotype threat about math actually do worse? Compare theories: Which best explains women’s statistics performance: stereotype threat or social role learning? Extend an established theory: Can stereotype threat help us explain athletic as well as academic performance? Apply a theory: Can I create instructions that relieve stereotype threat for women during statistics? 36 Foundations of Research Core features of research: Hypotheses. Phenomenon - Larger question the research addresses Theory - Basic Ψ processes we think explain the phenomenon Hypothesis - Concrete variables that express the Ψ processes - Specific prediction Hypothesis: Methods / Data - Operational definitions - Study procedures A prediction Results - Hypothesis-wise Derived from / testing thestatistical theory analysis of study outcomes That is potentially falsifiable. Discussion & Conclusion - Relate results back to theory - Study limitations & Future studies 37 Foundations of Research Hypothesis An hypothesis is a Prediction Relating variables derived from the theory. Specifies cause and effect. …that is potentially falsifiable (see text for discussion) Can be conceivably / logically shown to be untrue Specific enough to be tested 38 Foundations of Research Hypothesis Hypotheses: Measurement v. Experimental studies In experiments we manipulate the Independent Variable. Hypotheses are expressed in control terms: IF I make people relaxed then their fear and loathing of statistics will decrease. Measurement studies do not manipulate anything. Hypotheses are expressed as a relation. People who are high on a measurement of relaxation will tend to fear statistics less; I predict a correlation between relaxation & fear of stats. 39 Foundations of Research Core features of research: Methods. Methods: Phenomenon - Larger question the research addresses Theory We attempt to make term ofexplain the study as - Basic each Ψ processes we think objective as possible. the phenomenon Hypothesis Both measurement -&Concrete experimental variables that the Ψ processes procedures rely on express Operational Definitions - Specific prediction Methods / Data - Operational definitions - Study procedures Results - Hypothesis-wise statistical analysis of study outcomes Discussion & Conclusion - Relate results back to theory - Study limitations & Future studies 40 Foundations of Research Methods Turn our hypotheses into concrete variables That we examine via specific procedures Core element of scientific approach Objective; designed to separate data from person Public: multiple people participate in, challenge, or use scientific findings Replicable: others can repeat or expand the study 41 Foundations of Research 42 Methods We turn our hypotheses into variables via an operation definition. What is “depression”? What operations could assess it? Verbal behavior Depression Appearance “vegetative”; sleep, eating Suicide, drug use, work… Survey / questionnaire answers… Foundations of Research Methods Our Operation definition determines what a variable “means” in our study. What is “economic uncertainty”? The unemployment rate? Subjective attitude ratings? What is “Stress”? Heart rate & cortisol levels? Speech patterns? 43 Foundations of Research 44 Methods This is the independent variable To test the hypothesis that stress impairs memory I may create stress in the lab via… Shock. Requiring a difficult performance in front of others. Are shock (a physical threat) and performance (a social threat) Equivalent ‘Stressors”? Foundations of Research 45 Methods I may measure stress levels in this class via: A questionnaire scale Heart rate Anxious behavior, sleep loss, appetite change… Are Heart rate and sleep loss Equivalent measures of Stress? Foundations of Research 46 Operationalizing “Stress” Does stress lead to illness? EXAMPLE “Stress” “Illness” Measure the dependent Experimental Manipulate the variable independent variable design Threatening information. Infection postexposure Shock. Observed respiratory infections Require difficult public performance. Measurement Measure the predictor study Questionnaire scale on life events Assess cardiovascular reactivity Measure the outcome variable Self-reported respiratory infections Morbidity & mortality Foundations of Research Methods: Why use Operational Definitions? Any theory must be operationalized to be heuristically useful Operational definitions real observable world. I think stress makes people less healthy What does “stress” mean? How will you measure it? What does “health” mean? How will you measure it? Think critically about what each variable means. 47 Foundations of Research Some variables are easy to operationalize; e.g., the effect of a drug dose on hypertension. IV = drug dose1 v. drug dose 2 v. Placebo… DV = blood pressure. Some constructs can only be roughly operationalized. Methods: The limits of Operational Definitions “Pro-social attitude”, “self-concept”… Some constructs have diverging operational definitions. How do you operationally define “stress”? …learning? Behavior? Self-perception? Physiological? Some domains may not be operationalizable. String theory… “Spirituality”? “Happiness”? 48 Foundations of Research EXAMPLE When am I dead? 49 Middle ages: the soul departs the body – weighs 21 grams The name: • Physical death • Your body is consigned to the grave • Someone speaks your name the last time 17th Century: Cordelia’s daughter in King Lear shows no breath on a mirror held to her nose 19th Century: Development of the stethoscope and “heart death”. Mid-20th Century: Development of respirators / life support and “brain death”. 21st Century: fMRI images show responsiveness even in some “vegetative” patients Foundations of Research Core features of research: Results. Phenomenon - Larger question the research addresses Theory - Basic Ψ processes we think explain the phenomenon Results: Hypothesis - Concrete variables that express the Ψ processes - Specific prediction Qualitative Quantitative; statistical Methods /reasoning Data - Operational definitions - Study procedures Results - Hypothesis-wise statistical analysis of study outcomes Discussion & Conclusion - Relate results back to theory - Study limitations & Future studies 50 Foundations of Research Results: Two major streams: Qualitative research rich description Text, Images, Video… Quantitative research hypothesis tests Numerical scales Statistical reasoning 51 Foundations of Research Results: Quantitative research. Numerical representation of reality Descriptive statistics Inferential statistics Simple characterization: “who / what / when?” Generalize to a larger population. “Statistical reasoning”: Probability judgments using the Normal distribution. 52 Foundations of Research Core features of research Phenomenon - Larger question the research addresses Theory - Basic Ψ processes we think explain the phenomenon Hypothesis - Concrete variables that Discussion & Conclusions: express the Ψ processes - Specific prediction Methods / Data Implications for theory? - Operational definitions Study limitations?- Study procedures Results Where now? - Hypothesis-wise statistical analysis of study outcomes Discussion & Conclusion - Relate results back to theory - Study limitations & Future studies 53 Foundations of Research Discussion Study implications. What does it mean that the hypothesis was (was not) supported? Change / modify theory? Other interpretations / hypotheses? Applications? Critical Thought about the meaning – and alternate interpretations – of our results. 54 Foundations of Research Discussion Study limitations. Boundaries on what this study can tell us? Internal validity: Well did we represent the hypothetical constructs…? Quality / nature of operationalization & design? External validity: How representative was… Our sample? Manipulation / measurement of the independent variable(s)? Assessment of the dependent variable(s)? The research setting itself. 55 Foundations of Research Phenomenon & Theory Hypothesis SUMMARY 56 What does science do? Methods Results Discussion Hypothetical constructs In important relationship Specific variables Falsifiable prediction Operational definition Internal & external validity Qualitative / Quantitative Descriptive question or exploration Hypothesis test Meaning of results for the theory Alternate interpretations Study Limitations. Foundations of Basic Research 57 Elements of a Research Project Phenomenon Big picture / question Theory Hypothetical Constructs Causal explanation Hypothesis Begin with the “big question” … articulate a clear theory …derive concrete hypotheses. Operational definition Specific prediction Methods Measurement v. experimental Specific methods & operational definitions Data / Results Actual data & results… • Descriptive data • Test hypothesis Discussion … implications for the theory Implications for theory Conclusions Future research? …and larger issues. Foundations of Basic Research 58 Elements of a Research Project Phenomenon Big picture / question Theory Hypothetical Constructs Causal explanation Hypothesis Operational definition Specific prediction Methods Measurement v. experimental Data / Results • Descriptive data • Test hypothesis Discussion Implications for theory Conclusions Future research? Each element of the project corresponds to a later / earlier issue… Foundations of Basic Research Elements of a Research Project Study results often lead to the next experiment… 59 Phenomenon Big picture / question Theory Hypothetical Constructs Causal explanation 2. …what don’t we understand? 3. …explanation? Hypothesis Operational definition Specific prediction 4. Theory hypothesis Methods Measurement v. experimental 5. …how do we test or measure it? Data //Results Data Data Results • Descriptive data •Test hypothesis Descriptive data Test hypothesis or•observation Discussion Implications for theory Conclusions Future research? 1. Observation or hunch Then we run the rest of the process Foundations of Basic Research 60 Elements of a Research Project Phenomenon Big picture / question Research results new experiment or study. Theory Hypothetical Constructs Causal explanation Hypothesis 1. Alternate hypotheses? Unanswered questions? Operational definition Specific prediction Methods Measurement v. experimental Data / Results Results • Descriptive data Test newhypothesis hypothesis Was the • Test hypothesis supported? Discussion Implications for theory Conclusions Future research? 2. Negative results: Reframe hypothesis… …operational definitions 3. Run follow-up study. Foundations of Basic Research 61 Elements of a Research Project Phenomenon Big picture / question Theory Hypothetical Constructs Causal explanation 1. Findings may lead us to rethink our theory. Hypothesis Operational definition Specific prediction Methods Measurement v. experimental Results Other findings Data / Results Results Data / Results • Descriptive data Our new initial findings Test hypothesis • Test hypothesis Discussion Implications for theory Conclusions Future research? 2. We: Formulate different hypotheses Different study design & variables New results Foundations of Basic Research 62 Elements of a Research Project Phenomenon Big picture / question Theory Hypothetical Constructs Causal explanation Hypothesis Operational definition Specific prediction Methods Measurement v. experimental Data / Results • Descriptive data • Test hypothesis Discussion Implications for theory Conclusions Future research? Key elements of research: Not a simple linear process. All elements interact. Foundations of Research Elements of science, review 1 A hypothetical construct is: A = A concrete description of a variable B = An abstract statement about a ψ process that cannot be seen directly. C = An excuse you construct to explain why you are late. D = An abstract use of statistical theory to test a hypothesis. 63 Foundations of Research Elements of science, review 2 A theory is: A = Wild-eyed speculation about some topic that most people are not interested in. B = An authoritative statement of how something works: truth. C = Always tentative or provisional. D = A statement about how two (or more) hypothetical constructs are related. 64 Foundations of Research Elements of science, review 3 An operational definition is: A = The specific way we manipulate an independent variable. B = A surgical procedure we use to test a hypothesis. C = The particular procedures we use to measure a study variable. D = An abstract statistical statement using probability theory to test hypotheses. 65 Foundations of Research Elements of science, review 3 In science the numbers are what count most. A. Yes, if I have enough of them B. Yes, if they have been operationally defined C. No, the measures are most important D. No, the Theory and hypothesis is most important. 66 Foundations of Research Introduction to science, 6 What does science do? The core features of a research study. Overall Research approaches. 67 Foundations of Research Overall research strategies Observation or Measurement Simple Description Qualitative ‹#› 68 Quantitative Correlational Studies Experiments Quasiexperiments Measurement studies vs. Experiments “True” experiments Foundations of Research Overall research strategies Observation or Measurement Simple Description Qualitative ‹#› 69 Quantitative Correlational Studies Assess nature Describe behavior Test hypotheses Experiments Quasiexperiments “True” experiments Exert control over nature Test experimental predictions (hypotheses). Foundations of Research Overall research strategies Observation or Measurement Simple Description Qualitative Explore behavior. Rich / detailed description. Often targeted samples. ‹#› 70 Quantitative Describe trends. Count behaviors e.g., by age, gender, ethnicity... Archival data Experiments Correlational Studies Quasiexperiments “True” experiments Relate variables Field studies Controlled (“lab”) conditions. Measured variables Natural events Experiments: Test or generate No control hypotheses. over IV Nonequivalent Groups Manipulate Independent Variable Measure Dependent Variable. Control all aspects of experiment Foundations of Research Overall research strategies: Drug use Observation or Measurement Simple Description Qualitative ‹#› 71 Quantitative Correlational Studies Experiments Quasiexperiments “True” experiments Research Question: Research Question: Research Question: Research Question: Research Question: Mechanics of drug use. Epidemiology of drug use. Social / ψ variables in drug use. Compare drug treatments. What brain centers control “drug craving”? Methods: Methods: Methods: Surveys or interviews Archival data Experimental design. Methods: Methods: Directly observe drug markets, In-depth interviews drug users, Police… Surveys, interviews, archival data Stimulate contrasting brain areas (IV). Assess drugseeking (DV). Groups are non-equivalent not blind not randomly assigned… 72 Foundations of Research Controlled experiments: “Gold standard” for testing hypotheses. Participant Selection Group Assignment Experimental group Sample Random sample of the target population. Control group Randomly assign to groups. Experimenta l Procedures Experimenta l Condition Procedure “Treatment” condition Outcome Procedure Control / placebo condition Outcome Exactly = study procedures. Impose Exp. manipulation Independent Variable. Results Assess Dependent Variable. 73 Foundations of Research Participant Selection Group Assignment Experimental group Sample Random sample of the target population. Control group Randomly assign to groups. Experimenta l Procedures Experimenta l Condition Procedure “Treatment” condition Outcome Procedure Control / placebo condition Outcome Exactly = study procedures. Impose Exp. manipulation Independent Variable. Results Assess Dependent Variable. Foundations of Research Overall Research strategies: measurement v. experiments experiments: Many areas Controlled not amenable to true experiments, Education research “Gold standard” for testing hypotheses. Naturally occurring events Public health Experimenta Experimenta Key: degreeGroup of control over variables Participant Selection Assignment Experimental group Experiment Sample High control Control / ‘lab’group conditions Random sample of the target population. Randomly assign to groups. Measurement Less control; ‘research in nature’ l Procedures l Condition Procedure “Treatment” condition Internal Procedure validity Exactly = study procedures. External validity Results Outcome Determine “cause and Control / effect”: validly interpret placebo Outcome data condition Impose Exp. Assess manipulation Dependent to Data can generalize Independent Variable. “real world” & capture Variable. more complexity 74 Foundations of Overall Research strategies: Research ‹#› 75 Validity Observation or Measurement Simple Description Qualitative Quantitative Explore Describe Experiments Correlational Studies Quasiexperiments “True” experiments Relate measured variables Field studies. Test specific hypotheses External validity Internal validity Less control: Observe / test phenomenon under natural conditions. More accurate portrayal of: “how it works in nature” More control: Isolate (or create) the phenomenon in a controlled environment Addresses specific questions or hypotheses More able to interpret cause & effect complexity of phenomenon Less able to interpret cause & effect 76 Foundations of Research Overview Foundations of Research 77 Core course topics How do we know things? What does scientific method tell us that other methods (political, religious thought) do not? What does science do? Describe the world Taxonomies Epidemiology Qualitative research Test theories Predict events Simple predictions Correlational studies Experiments Cause & effect Identify basic processes Show how processes are related Test applications of theories E.g., behavioral interventions Foundations of Research key terms Features of research: Key terms Theory Hypothetical construct Hypothesis Variable Operational definition Experimental control Measurement v. experimental studies Internal & external validity 78 Foundations of Basic Research Elements of a Research Project Phenomenon Big picture / question Theory Hypothetical Constructs Causal explanation Hypothesis Operational definition Specific prediction Methods Measurement v. experimental Data / Results • Descriptive data • Test hypothesis Discussion Implications for theory Conclusions Future research? 79 Foundations of Research Observation or Measurement Simple Description Qualitative Explore the actual process of a behavior. 80 Basics of major forms of research. Quantitative Describe a behavioral or social trend. External validity Experiments Correlational Studies Quasiexperiments “True” experiments Relate measured variables to each other to test hypotheses. Test hypotheses in naturally occurring events or field studies. Test specific hypotheses via controlled “lab” conditions. Internal validity