How Organisms Obtain Energy Energy is the ability to do work Thermodynamics is the study of the flow & transformation of energy in the universe Laws of Thermodynamics First law—energy can be converted from one form to another, but it cannot be created nor destroyed. Second law—energy cannot be converted without the loss of usable energy Autotrophs and Heterotrophs Autotrophs use energy from sunlight or chemical bonds in inorganic substances to make organic compounds Heterotrophs are organisms that need to ingest food to obtain energy What was the best item you ate for lunch today? Metabolism All of the chemical reactions in a cell Anabolic reactions- simpler substances combine to make more complex molecules or store energy-usually requires energy Catabolic reactions-break down more complex molecules into simpler substancesusually releases energy-drives chemical rxns Biochemical Pathways Photosynthesis—light energy from the Sun is converted to chemical energy for use by the cell (anabolic) Cellular respiration—organic molecules are broken down to release energy for use by the cell (catabolic) Photosynthesis uses CO2 , water and sunlight (energy) to make organic compounds and O2 Cellular respiration uses organic compounds and O2 to make CO2, water and energy In 1648, a Flemish alchemist, Jan van Helmont, had a theory. To test it, he grew a tree in a tub of soil, adding nothing but measured quantities of water for five years. During that time he kept track of the weight of the soil and the tree. At the end of the experiment the tree had gained 164 pounds and the soil had lost 2 ounces. What could von Helmont conclude from his experiment? 140 120 130 30 40 50 60 70 10 480 5 6 7 8 9 180 20 160 90 170 150 100 110 Overview of Photosynthesis Photosynthesis occurs in 2 phases: Light reactions-light energy is absorbed and temporarily stored in ATP and NADPH Calvin Cycle (carbon fixation)organic compounds formed using CO2 and energy in ATP and NADPH What is an organic molecule? Build a carbon dioxide – what type of bonds does it have? 2. Build a water molecule- what type of bonds does it have? 3. Begin bonding the 6 carbons together for glucose-note 1 is not in the ring 1. Phase One: Light Reactions The absorption of light is the first step in photosynthesis How does a leaf absorb light? They contain light-absorbing pigments in the chloroplasts Chlorophyll the primary pigment in photosynthesis, absorbs mostly blue and red light and reflects green and yellow light • Plants contain two types of chlorophyll, chlorophyll a and chlorophyll b The pigments that produce yellow and orange fall leaf colors, also the colors of many fruits, vegetables, flowers, are called carotenoids. Carotenoids absorb wavelengths different from chlorophyll, both pigments allow plants to absorb more light energy Absorption Spectra of Photosynthetic Pigments Pigments are embedded in the membranes of thylakoidsphotosystem I and II When light strikes a thylakoid in a chloroplast, energy is transferred to electrons in chlorophyll. This energy transfer causes the electrons to jump to a higher energy level. The excited electrons that leave chlorophyll molecules must be replaced by other electrons Plants get replacement electrons from water molecules. Photolysis is the splitting of water by light form oxygen gas, O2 2H2O 4 H+ + 4e- + O2 ions (H+, protons) are released into the lumen of the thylakoid Here H+ ions build up and when H+ passes through ATP synthase into the stroma of the chloroplast, ADP + Pi are converted into ATP - chemiosmosis Hydrogen electrons are released into the electron transport system The excited electrons move from photosystem II to an electron-acceptor molecule in the thylakoid membrane The electron-acceptor molecule transfers the electrons along a series of electron-carriers to photosystem I Photosystem I transfers the electrons to a protein called ferrodoxin Ferrodoxin transfers the electrons and a proton to the electron carrier NADP+, forming the energy-storing molecule NADPH Phase Two: The Calvin Cycle-Carbon Fixation In the second phase of photosynthesis, called the Calvin cycle, energy is stored in organic molecules such as glucose Six CO2 molecules combine with six 5-carbon compounds to form twelve 3-carbon molecules called 3-PGA The chemical energy stored in ATP and NADPH is transferred to the 3-PGA molecules to form high-energy molecules called G3P Two G3P molecules leave the cycle to be used for the production of glucose and other organic compounds An enzyme called rubisco converts the remaining ten G3P molecules into 5-carbon molecules called RuBP These molecules combine with new carbon dioxide molecules to continue the cycle How does the essential CO2 get into the plant? How does O2 leave? Alternative Pathways C4 plants-corn, sugarcane, crabgrass – plants that keep stomata partially closed during the hottest part of the day CAM plants-cacti, orchids, pineapplesstomata only open at night, grow slowly but lose little water Factors that affect Photosynthesis Light Intensity CO2 level Temperature All increase photosynthesis as factor increases to a terminal point