ENERGY & ENZYMES LIFE PROCESSES REQUIRE ENERGY • Energy = the ability to move or change matter. What are some forms of energy? • Light • Heat • Chemical • Electrical From food webs to the life of a cell energy energy energy Flow of energy through life Life is built on chemical reactions transforming energy from one form to another organic molecules ATP & organic molecules sun solar energy ATP & organic molecules organic molecules ATP & organic molecules Chemical Reactions • Process in which bonds between atoms are broken, and new ones are formed. • This produces one or more different substances. Chemical Equations Reactants: The starting materials for a chemical reaction. Products: The substances that form after the reaction. Follow a specific format • Reactants • NaCl Products Na + Cl+ Metabolism All of the chemical reactions that occur within an organism. Metabolism Chemical reactions of life Bond must form between molecules Occurs through dehydration synthesis Dehydration synthesis: A chemical reaction that builds up molecules by losing water molecules. Anabolic reactions – Building up molecules from smaller units - Requires an input of energy That’s why they’re called anabolic steroids! Metabolism Bonds also must break between molecules Occurs through hydrolysis Hydrolysis: chemical reaction that breaks down molecules by adding water molecules e.g. starch into glucose Digestion Catabolic reactions – Breaking down of molecules into smaller units 11 Chemical reactions & energy Some chemical reactions release energy exergonic digesting molecules= LESS organization= lower energy state Some chemical reactions require input of energy endergonic building molecules= MORE organization= higher energy state 13 Energy is needed to start a chemical reaction ENERGY • Activation Energy: The energy needed to start a chemical reaction. Enzymes help reactions occur • Enzymes: substances that increase the speed of chemical reactions. • http://www.youtube.com/watch?v=XT Um-75-PL4 Enzymes: • are Proteins • are Catalysts • are in YOUR cells catalyzing reactions RIGHT NOW • Help you maintain homeostasis • Are specifically shaped by type • Work on substrates • Substrate: substance upon which enzymes work • Have active sites (part the substrate fits into) Some common enzymes: • Amylase • Catalase • Sucrase • DNA Polymerase • Lipase • Protease Naming conventions Enzymes named for reaction they catalyze sucrase breaks down sucrose proteases break down proteins lipases break down lipids DNA polymerase builds DNA adds nucleotides to DNA strand pepsin breaks down proteins (polypeptides) Activation Energy with and without an enzyme How does an enzyme work? • Step 1: Enzyme attaches to its specific substrate (sucrase attaches with sucrose) • Step 2: At the active site, the enzyme and the substrate interact in a way that reduces activation energy of the reaction • Step 3: The reaction is complete when products form. The enzyme is now free and goes an acts on a new substrate. Lock and Key model Simplistic model of enzyme action substrate fits into 3-D structure of enzyme’ active site H bonds between substrate & enzyme like “key fits into lock” Some things can make enzymes work FASTER or NOT AT ALL • Temperature: Enzymes work at optimal temperatures. If the temperature is too high or too low, it may change the shape of the enzyme so it won’t work. • pH: Values outside of the preferred range of enzymes may cause bonds to break and then the enzyme can’t work. Temperature reaction rate What’s happening here?! 37° temperature Enzymes and temperature Different enzymes function in different organisms in different environments reaction rate human enzyme hot spring bacteria enzyme 37°C temperature 70°C (158°F) pH What’s happening here?! trypsin reaction rate pepsin pepsin trypsin 0 1 2 3 4 5 6 pH 7 8 9 10 11 12 13 14