Chapter 4- Cell Processes ATP Photosynthesis Cellular Respiration Fermentation By: Mrs. Stahl Biology Unit Layout Background knowledge Energy- where does it all come from? Review of Ecological Terms Food webs, food chains, and the transfer of energy in ecosystems. All starts with sunlight and plants. ATP and ADP processes Photosynthesis Anatomy and functions of a plant. Process of photosynthesis and the importance of the chloroplast. Cellular Respiration and Fermentation Bringing Photosynthesis and Cellular Respiration and the relationship they have together. How do we get our energy? Chemical energy- starts with the sun! Two Main Sources of Energy: Lipids Carbohydrates Energy is only useable after it is broken down by a series of chemical reactions Energy………… •Energy for living things comes from food. •Originally, the energy in food comes from the sun and travels up the food web or food chain. Let’s review some terminology!! Ecology- The study of living things and their surroundings. Organism- individual living thing. Species- a group of organisms that can reproduce together and produce fertile offspring. Ex- humans are the same species. Population- group of the same species. Ex- A group of bottlenose dolphins. Community- group of different species living together. Exampledeer, rabbits, and birds. Ecosystem- Made up of both biotic and abiotic factors. Example- rocks, water, deer, rabbits Biome- A region or area that is defined by the climate and plants that grow there. Example- Tropical Rain Forest. Biosphere- Planet Earth Biodiversity- Variety of life Abiotic- Non-living things. Ex- water, sunlight, rocks Biotic-Living things. Ex- Plants and animals Keystone Species- species that keeps an ecosystem in check / holds it together. Example- Sea otters keep the sea urchin population in check so that they don’t eat all the kelp (algae). Producers / Autotrophs- make their own food via sunlight. Example- Plants Consumers / Heterotrophs- rely on others for food. Example- Animals Autotrophs Heterotrophs Types of Consumers Herbivores- Eat only plants Carnivores- Meat eaters Omnivores- Eat plants and animals Detritivores- Eat detritus or dead organic matter. Decomposers- Breakdown dead organic matter into simpler compounds. Herbivores Carnivores Omnivores Detritivores & Decomposers Energy Flow In ecosystems, energy has to flow from one organism to another, and it does this through food chains and food webs, starting with the sun and plants! Food Web Network of feeding relationships between trophic levels in an ecosystem. Arrows point in the direction which the energy is flowing. Food Chains Shows the feeding relationships for a single chain of producers and consumers. Rabbit eats the grass and the hawk eats the rabbit. How does the energy from the sun flow through an ecosystem? Trophic levels Trophic levels are nourishment levels in a food chain. Example- Producer- HerbivoreCarnivore = 3 Trophic levels Carnivores are the highest, herbivore are second, and producers are the first. Break it down further… – Primary consumers are herbivores that eat producers. – Secondary consumers are carnivores that eat herbivores. – Tertiary consumers are carnivores that eat secondary consumers. – Omnivores, such as humans that eat both plants and animals, may be listed at different trophic levels in different food chains. Trophic Levels Shark Triggerfish Shrimp Plants, algae, phytoplankton How does the energy get distributed from trophic level to trophic level? We know that ecosystems get their energy from sunlight, which then provides the energy for photosynthesis to occur. That energy then flows up the food chain. The amount of energy that gets transferred from trophic level to trophic level is 10% = Biomass 5 tertiary consumers secondary consumers primary consumers 5000 Carnivores eat herbivores and more energy is lost 500,000 Herbivores eat plants but burn some energy in the process Energy given off as heat producers producers 5,000,000 Producers use 100% of energy from the sun How does life continue? The sun pumps more energy into the plants allowing life to carry on. How do organisms lose energy? Metabolism Maintaining homeostasis- keeping your body at normal temperature Mating, finding food, resting, movement, growth, The same way we use energy so do other organisms. That’s why we have to continuously eat. Unused material = excreted as waste Example Grass- Prairie Dog- Coyote 1st- Grass=Photosynthesis traps energy as carbohydrates = ENERGY! 2nd Prairie dog eats the grass. The prairie dog uses some of the energy to grow, some is used to fuel cellular respiration. The loss of energy between levels may be as much as 90%, meaning that only 10% of energy is left over. Some fun review! http://www.youtube.com/watch?v =WLk-9ib0OVA http://www.youtube.com/watch?v =GUY_-LK_lOc ATP AND ADP- OUR MAIN ENERGY CURRENCY phosphate removed ATP- Adenosine Triphosphate Molecule that transfers energy from the breakdown of food molecules to cell processes. Starch molecule Glucose molecule Cells use ATP to: 1. Carry energy 2. Build molecules 3. Move materials by active transport ATP is made up of: Sugar ribose Adenine Three Phosphates ATP has 3 phosphate groups: Third bond is unstable so it is easily broken When 3rd is removed it’s releasing energy and turns into ADP How is ATP made? Breakdown of sugars Starch molecule Glucose molecule How are sugars made? By capturing energy from sunlight and changing it into chemical energy stored in sugars. How does ATP work Exactly????? Step 1- The energy carried by ATP is released when a phosphate group is removed from the molecule. The third bond is unstable and is easily broken. Step 2- Reaction takes place and the energy is released for cell functions, meaning the third phosphate fell off. Step 3- ATP (high energy) then becomes ADP (lower energy molecule) because it just lost a phosphate. Step 4-The molecules get broken down and energy gets added. Step 5- Phosphate is added and it’s back to ATP! phosphate removed What is needed to change ADP into ATP? Large group of complex proteins and a phosphate Why is this important? The foods that you eat don’t contain ATP. The food needs to be digested and broken down Everything that you eat has a different calorie amount (measures of energy), therefore different foods produce different amounts of ATP. The number of ATP produced depends on what you eat- Carbohydrates, proteins, or lipids. Swallow your food and then digestion takes place (NOT THAT FAST OF COURSE!). Does each type of food have the same amount of calories? - NO!!! - Different foods have different calories, therefore provide different amounts of ATP. Carbohydrates Carbohydrates are not stored in large amounts in your body because they are the most commonly broken down molecule. The breakdown of glucose yields 36 ATP. Carbohydrates DO NOT provide the body with the most ATP. Lipids do! Lipids Store the most energy, about 80% of the energy in your body. When they are broken down they yield the most ATP, 146 ATP Proteins Store about the same amount of energy as carbohydrates, but they are less likely to be broken down to make ATP. The amino acids that cells can break down to make ATP are needed and used to build new proteins. Summary The number of ATP molecules depends on the number of carbohydrates, lipids, or proteins broken down. The organic compound most commonly broken down to make ATP = carbohydrates. Fun Video https://www.youtube.com/watch? v=V_xZuCPIHvk http://www.youtube.com/watch?v =xUpuuL24NiQ http://www.youtube.com/watch?v =XI8m6o0gXDY We know that plants use photosynthesis, but what about organisms that live in the deep sea, where there isn’t any sunlight? Chemosynthesis Some animals don’t need sunlight & photosynthesis as a source of energy. Chemosynthesisprocess by which organisms use chemical energy to make their food. Example- Deep Ocean Hydrothermal Vents. https://www.youtube.com/watch ?v=XotF9fzo4Vo Mind Map Produced when phosphate is added to ADP Energy Carrier ATP Releases energy when converted back to ADP Gets energy from the breakdown of food molecules Do plants need ATP? YES!!!!!! Plants make their own food through photosynthesis where they breakdown sugars -> ATP Photosynthesis Defined as the process that captures energy from sunlight to make sugars that store chemical energy. Location- Chloroplast of plant cells. Photosynthesis Chloro= Green chloroplast Phyll= Leaf Plast = Molded leaf cell leaf Chloroplast Leaf Cell Leaf Anatomy of a Flower Female Parts Pistil= made up of the stigma, style, ovule, and ovary. Stigma- Sticky portion that catches the pollen. Style- tube that allows sperm / pollen to be transported. Ovary- becomes the fruit Ovule- where the seed develops Male parts Stamen- male parts made up of the anther and the filament. Anther- Produces the pollen Filament- Support tube for the anther Sepals- green, tough region that protects the flower before it opens. Receptacle- hard, base of the flower, bears the organs of the flower Stem- support, transports water and nutrients Two Types of Seed Plants Angiosperms Reproduce with structures called flowers and fruits. Brightly colored / highly scented Attract animals-> transported from place to place via pollination, feces, and wind Gymnosperms Conifers- cone bearers like pine trees. Naked seeds that aren’t enclosed in a fruit. Needle shaped leaves with a protective cuticle. Rely on wind for pollination. Angiosperms Gymnosperms Leaves Major site of photosynthesis / food production. Minimize water loss by collecting water and transpiration. Take in carbon dioxide and produce oxygen through the stomata. Stomata’s are tiny pores in the leaf. Protects stems and roots with shade and shelter. Basic Structure Blade- collects the sunlight Petiole- stem that holds the leaf blade up. Upper portion / Top of the leaf The tissue mesophyll, has most of the chloroplasts and is where the majority of the photosynthesis takes place. Bottom portion of the leaf / underside Has a stomata and is the site of transpiration and gas exchange. Guard cells surround each stomata and open and close by changing shape. Day- stomata is open, allowing the carbon dioxide to enter and water to evaporate. Night- close Guard Cells Modified epidermal cells that are photosynthetic and they open and close the stomata. Potassium ions accumulate in the guard cells and when there is a high concentration of K+ it causes water to flow into the cells. When the plant is full of water, the guard cells plump up and open the stomata. Factors that affect the stomata and guard cells Temperature, humidity, hormones, and the amount of carbon dioxide in the leaves tells the guard cells to open and close 4 Types of Plant Tissues 1. Ground Tissue 2. Dermal Tissue 3. Vascular Tissue 4. Meristematic Tissue Ground Tissue most common and they differ based on their cell walls- 3 Types 1. Parenchymal 2. Collenchymal 3. Sclerenchymal Parenchymal Cells The most common plant cell typemesophyll Cell walls store and secrete starch, oils and water Help heal wounds to the plant Have thin, flexible walls Collenchyma Cells Provide support to a growing plant They are strong and flexible. Celery strings are strands of collenchyma. They have unevenly thick cell walls. Sclerenchyma cells Strongest, support, very thick cell walls Second cell wall hardened by lignin Die when they reach maturity Used by humans to make linen and rope Dermal Tissue Covers and protects the outside Secretes cuticle of leaves Forms outer bark of trees= dead dermal cells Epidermis= covers the surface, made up of parenchymal cells Guard cells= surrounds the stomata and has a cuticle that secretes a waxy substance for protection. Vascular TissueXylem & Phloem Transports water, minerals, nutrients, and organic compounds to all areas of the plant. Made up of two networks of tubes- xylem and phloem. Phloem Carries the products of photosynthesis through the plant via active transport (products = oxygen and glucose). Remember- PHLOEM IS FOR FOOD Part of the bark (at or near) Have little sieve tubes and plates that help the fluid flow from one cell to another. Xylem Carries water and nutrients from the roots to the rest of the plant. Found within the wood of the tree. Tracheids- long, thin, overlapping cells with tapered ends. Vessel Members- wider, shorter, thinner cell walls. Meristematic Tissue Growth tissue Where cell division occurs Turns into ground, dermal, or vascular Apical Meristems- tips of roots and stems-> primary growth occurs here. Lateral Meristems- secondary growth. Increase the thickness of roots and stems. Seeds Monocots= one seed Dicots= two seeds Seed coat= protection Embryo Epicotyl- top, shoot tip Hypocotyl- attached to the cotyledon, young shoot Radicle- first organ from the germinating seed-> becomes the root. Cotyledon or Endospermstores food for the embryo Environmental cues that are required by the seed: Water, light, and temperature The seed is mature -> goes into a dormant stage until all environmental needs are met. Germination allows the seed to turn into a plant: 1st- Water is absorbed 2nd- Enzymes get triggered 3rd- Chemical process= respiration 4th- Water gets absorbed, causes the seed to swell and the seed coat cracks. 5th- Roots grow from the radicle and anchor the seedling into the soil. Hypocotyl grows to produce a young shoot. Roots and Stems Absorb nutrients Anchor the plant (hold it down) Store food They have specialized organs to carry these out. Root Organs 1. Epidermis- covers the outside surface of the root Has root hairs= increases surface area and allows for more water to be absorbed. They are constantly being replaced. 2. Cortex- makes up most of the root-> stores starch (sugars) in the parenchymal cells. 3. Endodermis- tightly packed ring of cells. Has suberin, a waxy band that surrounds each endodermis cell in a barrier where water can’t pass through called the Casparian Strip-> controls the movement of water and minerals. Root Growth Root Cap- tip, protects the apical meristem where primary growth occurs. Stems Support leaves and flowers Move water and food Fun Tree Rings Type of secondary growth Form due to uneven growth over the seasons. Age of the tree is done by counting the rings Lighter cell bands =spring growth Darker bands = later season growth During good growing seasons the rings are thicker Physiological Process of Transpiration, Photosynthesis, and Cellular Respiration Transpiration Evaporation of water from leaves Water is pushed up through the xylem by root pressure created from water moving up the soil to the plants root system and into the xylem-> results in small droplets of sap-> called guttation. Water is also pulled up through cohesion through the xylem tissue-> creates a negative pressure or tension from roots to leaves. Rate of Transpiration Slows in high humidity Accelerates or speeds up in low humidity Increases with wind Increases with intense light= increased photosynthesis and water vapor Photosynthesis Process of using sunlight as energy to make carbon compounds (glucose) to make food. Occurs in the chloroplast Two processes: Light reactions and Light independent reactions Equation Functions of Photosynthesis 1. Biochemical Process 2. Plant Cells only 3. Plant growth and development 4. Builds plant cell walls= cellulose 5. Helps regulate the Earth’s environment 6. Removes CO2 from the air Chloroplast Three main parts are: Grana- stacks of coined shaped membranes. Thylakoid Inside the grana and they are the little disks. They contain chlorophyll and other light absorbing pigments. Photosystems- light collecting units. They proteins that organize chlorophyll and other pigments into clusters. Stroma Fluid that surrounds the grana inside the chloroplast. Label the one in your notes!!!! Chlorophyll- the molecule in the chloroplast that absorbs the energy from the sunlight. Two main types chlorophyll a and b that absorb mostly red and blue light. Other pigments absorb the green. Green color in plants comes from the reflection of light’s green wavelengths by chlorophyll. Don’t have to put this in your notes!!! Just a little fun fact! Carotenoids are yelloworange pigments which absorb light in violet, blue, and green regions. When chlorophyll breaks down in fall, the yelloworange pigments in leaves show through. Fall Foliage So let’s begin The sunlight hits the leaves and CO2 is let in through the stomata (little pores) while H2O is let in through the roots. Photosynthesis is broken down into two different reactions!!! 1st Light Dependent Reactions or Light Reactions – Requires sunlight – Take place in thylakoids – Water and sunlight are needed – Chlorophyll absorbs energy – Energy is transferred along thylakoid membrane then to light-independent reactions – Oxygen is released 2nd Light Independent Reactions Uses the energy transferred from the light dependent reactions to make sugars. Reactions occur in the stroma Does NOT require sunlight Carbon dioxide is absorbed and used at this stage. Calvin Cycle- metabolic pathway found in the stroma of the chloroplast in which carbon enters in the form of CO2 and leaves in the form of sugar. ATP is produced as a final step and the enzyme ATP synthase is responsible for making ATP by adding phosphate groups to ADP. The whole process in simple terms………. Step 1- Chlorophyll absorbs energy from sunlight. Energy is transferred along the thylakoid membrane, water molecules are broken down, and oxygen is released. Step 2- Energy carried along the thylakoid is transferred to molecules that carry energy, like ATP Step 3- CO2 is added and larger molecules are built. Step 4- A molecule of simple sugar (glucose) is formed. Calvin Cycle Questions to review 1. Where do the light dependent reactions occur? 2. Where do the light independent reactions occur? 3. What two reactants are shown entering the chloroplast? 4. What two products are shown leaving the chloroplast? 5. What does the Calvin Cycle produce? Answers 1. Thylakoid membrane 2. Stroma 3. Water and carbon dioxide 4. Oxygen and sugar 5. Sugar- converts CO2 into sugar Videos http://www.youtube.com/watch? v=lDwUVpOEoE4 Now that we have a brief overview let’s look at it in a little more detail. Light Dependent Reactions Main function: capture and transfer energy Broken down into Photosystem 2 / Electron Transport and Photosystem 1 / Energy carrying molecule. Water molecules are broken down into hydrogen ions, electrons, and oxygen gas. Oxygen is a waste product and sugars are not made at this point. Energy is transferred to electrons. Electrons are used for energy during photosynthesis NOT for the cells general energy needs. Light Dependent Cont. Electron Transport Chain (ETC)- series of proteins in the membrane of the thylakoid. Energy-> electrons->ATP and NADPH (transferred to the later stages) Arrows represent energy and enzymes! NADP= coenzyme that can accept hydrogen and acts as an enzyme http://www.biologyonline.org/dictionary/Nicotinamide_adenine_di nucleotide_phosphate Lets put it all together, first stop Photosystem 2 and ETC Step 1-> Energy is absorbed from sunlight via chlorophyll and other pigments. Energy is transferred to the electrons which enter the ETC. Step 2-> Water molecules are broken down by enzymes and oxygen is released as waste. Step 3-> Electrons jump from protein to protein down the ETC and their energy is used to pump the Hydrogen ions from outside to inside the thylakoid membrane (against the concentration gradient = ACTIVE TRANSPORT) Photosystem 1 and Energy Carrying Molecules Step 4-> Energy from sunlight continues to be absorbed, energizing electrons and pushing them along the ETC. Step 5-> Electrons are then added to the molecule NADP+ (functions like ADP) to produce NADPH (functions like ATP). Step 6-> Hydrogen ions diffuse through a protein channel. Step 7-> ATP is produced. ADP is changed into ATP when hydrogen ions flow through ATP synthase (enzyme). Now that we have completed all the steps of the light reactions, now we need to finish the process of photosynthesis with the dark reactions or light independent reactions! Calvin Cycle Light Independent / Calvin Cycle Uses the ATP from light dependent reactions. ATP is crucial because without it the reaction would not happen. Does not need sunlight Occurs in the stroma and produces sugars Energy sources are ATP and NADPH Energy that is needed for a series of chemical reaction is called the Calvin Cycle, named after the scientist- Melvin Calvin. Light Independent / Calvin Cycle continues * A molecule of glucose is formed as it stores some of the energy captured from sunlight. Carbon dioxide molecules enter the Calvin cycle Energy is added and carbon molecules are rearranged A high-energy three-carbon molecule leaves the cycle Steps in Detail…. 1. CO2 is added to the 5 carbons that are already there making a 6 carbon sugar. 2. Energy is added. ATP and NADPH is used from LDR to split the six carbons into 2 groups of 3, and to keep the cycle going. 3. Three carbon molecules exit. After they both exit they bond together to form glucose. 4. Three carbon molecules are recycled and changed back to five carbon molecules by energy from ATP. It takes two turns of the Calvin Cycle to produce 1 molecule of glucose. Review Questions 1. Where do the light reactions occur? 2. Where do the electrons come from in the ETC? 3. What role do these electrons play? 4. What two energy carriers are produced? 5. When does active transport take place? 6. What enzyme speeds up the process? 7. Where in the chloroplast do light independent reactions occur? 8. Where does the ATP and NADPH come from for the light independent reactions? 9. What does the LDR make? What does the LIR make? 10. How many cycles or turns does it take to make one glucose molecule? Answers 1. Thylakoid membrane 2. Chlororphyll 3. Provide energy to move hydrogen ions into the thylakoid and to produce molecules of NADPH 4. NADPH and ATP 5. Step 3 when hydrogen ions are transported 6. ATP synthase 7. Stroma 8. LDR 9. LDR= makes ATP, LIR= makes sugars 10. 2 Let’s Summarize Write the Equation for Photosynthesis Process Light Dependent Reactions Where the photosystems take place. Light Independent Reactions. Where the Calvin Cycle takes place Location Reactants Ending Products Let’s Summarize 6CO2 + 6H2O -> C6H12O6 + 6O2 Process Location Reactants Ending Products Light Dependent Reactions Thylakoid Membrane Sunlight H2O ATP NADPH O2 Stroma ATP NADPH CO2 Glucose Where the photosystems take place. Light Independent Reactions. Where the Calvin Cycle takes place Videos http://www.youtube.com/watch?v= k17bJQSQeQ4 Now we take photosynthesis and see how we, HUMANS and other organisms use it through a process called Cellular Respiration. Cellular Respiration!! Releases chemical energy from sugars and other carbon based molecules to make ATP when oxygen is present. By the time you reach 16 you have taken about 200 million breaths FUN FACT! Animals use cellular respiration Plants use photosynthesis Breakdown food-> ATP Aerobic-> Need Oxygen Anaerobic= no oxygen Takes place in the Mitochondria After you eat and the food is broken down into glucose then the glucose needs to get broken down by glycolysis (2-3 carbon chains, ATP), which takes place in the cytoplasm and is anaerobic. Glycolysis Glycolysis Ongoing process in all cells Happens before cellular respiration in the cytoplasm, outside the mitochondria Anaerobic= no oxygen required Makes a small number of ATP molecules. Makes 4 ATP, BUT it uses 2 to split up the carbons, therefore only 2 ATP molecules enter the mitochondria. Series of reactions converts the three-carbon molecules to pyruvate / pyruvic acid. Pyruvate and NADH are used for cellular respiration. What is pyruvate? Our bodies actually make it naturally during metabolism and when we digest sugars and starches. It is crucial for the Kreb’s cycle in cellular respiration. 2 Stages Stage 1= Krebs Cycle Stage 2= Electron Transport Krebs Cycle Main function- transfer high energy electrons to molecules that carry them to the ETC Occurs in the matrix of mitochondria Also known as the Citric Acid Cycle because it’s the first molecule formed. 1 mitochondrion ATP matrix (area enclosed by inner membrane) and 6CO 2 energy 2 3 energy from glycolysis inner membrane ATP and and 6O 6H O 2 2 4 Step 1 Pyruvate is broken down into 2 carbon molecules and CO2 is released as a waste product. NADH is produced Step 2 Coenzyme A bonds to the 2 carbon molecule made from pyruvate and enters the Kreb’s Cycle. Acetyl-CoA is one of the most important molecules in the body because all nutrients (carbs, lipids, and proteins) generate it when they break down. This molecule is produced in large amounts and is pumped into the Kreb’s cycle if the body is in need of energy, or into synthesis of fat to be stored for later use. Step 3 Citric Acid is formed- the two carbon molecule binds with a four carbon molecule to make a six carbon molecule which is called citric acid. Step 4 Citric acid is broken down NADH is made CO2 is given off as a waste product. Step 5 Five carbon molecule is broken down Four carbon molecule, ATP, and NADH are formed. NADH leaves the Krebs cycle Step 6 Four carbon molecules are rearranged High energy electrons are released NADH and FADH2 (electron carrier) are made One Molecule of Pyruvate makes these products: 3 molecules of CO2 have been given off 1 molecule of ATP 4 molecules of NADH2 to the ETC 1 molecule of FADH2 to the ETC If Glycolysis produces 2 molecules of pyruvate, how much of each product do we have???? Answer 6 molecules of CO2 have been given off 2 molecules of ATP 8 molecules of NADH2 to the ETC 2 molecules of FADH2 to the ETC Electron Transport Chain Takes place along the inner membrane of the mitochondria Made up of proteins Proteins use energy from NADH and FADH2 to pump hydrogen ions against the gradient (active transport) Step 1 Proteins take electrons. They take 2 NADH and 1 FADH2. Step 2 Proteins use energy from the electrons to pump the hydrogen ions through the inner membrane and the hydrogen ions build up on the inside of the membrane. Step 3 ATP is produced Flow of hydrogen ions helps make the ATP ATP synthase adds phosphate groups to ADP to make the ATP molecules. For each pair of electrons that passes through 3 ATP molecules are made. Step 4 Oxygen enters and water is formed. Water is given off as a waste product End Result / Products of Cellular Respiration CO2 and pyruvate (from Kreb’s) H2O from the ETC Net gain of about 38 ATP molecules are made from 1 glucose molecule-> 2 glycolysis 2 from Kreb’s Cycle 34 from the ETC Photosynthesis Cellular Respiration Location Chloroplast Mitochondria Reactants CO2 and H2O C6H12O6 and O2 Products C6H12O6 and O2 CO2 and H2O Electron Transport Chain Proteins within the thylakoid membrane Proteins within the inner mitochondrial membrane Cycle of chemical reaction Calvin cycle in the stroma of chloroplasts builds sugar molecules. Krebs cycle in matrix of mitochondria breaks down carbon based molecules. What happens to your cells when there isn’t enough oxygen to keep cellular respiration going? Fermentation Allows glycolysis to continue Does NOT make ATP Removes electrons from NADH and recycles NAD+ Important for NAD+ to still pick up electrons because if it didn’t, glycolysis would stop and wouldn’t be able to make ATP. Lactic Acid Fermentation Occurs when oxygen is unavailable Causes your muscles to be sore / burn When oxygen is available your cells return to using cellular respiration and the lactic acid is broken down / removed. This is why you breathe heavy after exercising and it takes a few minutes to recover because your body is trying to recover from the oxygen depletion in your muscle cells. Alcoholic Fermentation Forms the same way as the other two: Glycolysis splits a molecule of glucose to make 2 ATP, 2 pyruvate, and 2 NADH molecules. Occurs in many yeasts- CO2 causes the dough to rise End product is CO2, NAD+, and ethyl alcohol Fermentation is used in food production. Yogurt Cheese Bread