Campus Location:
Wilmington
Effective Date: 2013-52
Course Number and Title:
HTT 212 - Histotechnology Procedures II
Prerequisite(s):
HTT 211
Course Credits and Hours:
3 credits
2 class hours/week
3 lab hours/week
Course Description:
Part II of Procedures (HTT 211)
Introduction to advanced techniques and special procedures. Students
will learn procedures for cytology prep, intro to cytogenetics, muscle
enzyme histochemistry, immunohistochemistry and molecular histology.
The course will include tissue preparation, staining technology, quality
control and trouble shooting, for these more advanced techniques.
Required Text(s):
Obtain current textbook information at
www.dtcc.edu/student-resources/bookstores or by visiting the Bookstore.
You will need to know the course number and section to purchase or
obtain information.
Additional Materials:
Instructor Handout
Method of Instruction:
Face-to-Face
Disclaimer:
Core Course Performance Objectives:
1. Distinguish the basic principles and procedures for cytology technique for a Histologist.
(CCC 1, 2; PGC 1, 2, 4, 6, 7)
2. Demonstrate understanding of enzyme histochemistry theory and techniques. (CCC 1, 2, 6, 7;
PGC 2, 4, 5, 6, 7)
3. Demonstrate understanding of immunohistochemistry theory. (CCC 1, 2; PGC 2, 3, 4, 6, 7)
4. Demonstrate understanding of immunohistochemistry procedures. (CCC 1, 2; PGC 2, 4, 6, 7)
5. Demonstrate basic knowledge and understanding of molecular histology and discuss
basic Laser Capture Microdissection (LCM) and Tissue Microarray (TMA) procedures.
(CCC 1, 2, 6; PGC 2, 4, 6, 7)
6. Demonstrate professional conduct. (CCC 1, 3, 4; PGC 8, 9, 10, 12)
See Core Curriculum Competencies (CCC) and Program Graduate Competencies (PGC) at the end
of the syllabus. Course objectives are coded to the competency(cies) they develop.
Measurable Performance Objectives:
The student will be able to:
1. Distinguish the basic principles and procedures for cytology technique for a Histologist.
1.1 Demonstrate knowledge of the methods for obtaining samples, preparation of smears,
fluids and washing for exfoliative cytology.
1.2 Discuss methods for handling samples with excessive blood or mucous and
concentrating cells.
1.3 Outline staining techniques for exfoliative cytology and cytogenetics.
1.4 Identify basic cell types and describe normal karyotype preparation.
1.5 Define the sources of error and means for quality control.
1.6 Perform special techniques for exfoliative cytology in accordance with written
methods and obtain acceptable results.
2. Demonstrate understanding of enzyme histochemistry theory and techniques.
2.1 Describe and identify skeletal muscle, fiber types, and staining pattern in muscle
histochemistry.
2.2 List the basic histochemical methods for enzymatic muscle staining.
2.3 Identify artifact from true staining in histochemical staining procedures.
2.4 Outline the basic procedures for preparing muscle for histochemical, EM, and
Biochemical analysis.
2.5 Document the method used to obtain muscle histograms.
2.6 Perform all phases of muscle enzyme histochemistry procedures and obtain
acceptable results.
a. Operate and maintain a Cryostat and pH meter.
b. Prepare solutions and follow written protocols for histochemistry and
immunohistochemistry.
c. Evaluate, interpret, and record histological staining results.
3. Demonstrate understanding of immunohistochemistry theory.
3.1 Define and show basic understanding of basic immunohistochemistry terminology.
3.2 List and describe the different methods for immunohistochemistry.
3.3 Identify the difference in detection methods used for immunohistochemistry.
3.4 Select appropriate controls for immunohistochemistry.
3.5 Demonstrate knowledge of determining antibody titers.
3.6 Perform immunohistochemistry procedures by fluorescent and peroxidase
methodologies and obtain acceptable results.
a. Process, embed, cut, and stain paraffin and frozen sections.
b. Select and prepare appropriate secondary/link antibodies for
immunohistochemistry.
4. Demonstrate understanding of immunohistochemistry procedures.
4.1 Provide knowledge and use methods for enzyme-induced epitope retrieval in
immunohistochemistry.
4.2 Provide knowledge and use methods for heat-induced epitope retrieval in
immunohistochemistry.
4.3 Enumerate alternate methods for pre-treating, blocking and un-masking tissue for
immunohistochemistry.
4.4 Demonstrate knowledge Fluorescent and Peroxidase methods / direct, indirect and 3
step methods for immunohistochemistry and the use of control.
4.5 Troubleshoot and apply enhancement methods for immunohistochemistry.
4.6 Perform special techniques for immunohistochemistry procedures.
a. Select and use methods for enzyme-induced epitope retrieval and heat-induced
epitope retrieval.
b. Troubleshoot weak or background staining.
5. Demonstrate basic knowledge and understanding of molecular histology and discuss
basic Laser Capture Microdissection (LCM) and Tissue Microarray (TMA) procedures.
5.1 Using molecular terminology compare in situ hybridization methodology to
immunohistochemistry procedures.
5.2 Describe probe-labeling methods for in situ hybridization.
5.3 Select appropriate Controls for in situ hybridization.
5.4 Describe the technique for Laser Microdissection.
5.5 Describe Tissue Microarray procedures.
a. Describe uses for Tissue Microarray
b. Describe procedures for Tissue Microarray
6. Demonstrate professional conduct.
6.1 Demonstrate professional ethics.
6.2 Demonstrate punctuality in assignments and attendance.
6.3 Exhibit appropriate interpersonal skills with students, patients, visitors, and all other
clinical, hospital, or college personnel.
6.4 Adhere to all DTCC and clinical site policies.
6.5 Follow all universal and safety policies.
Evaluation Criteria/Policies:
Students will demonstrate proficiency on all Core Course Performance Objectives at least to the 75
percent level to successfully complete the course. The grade will be determined using the College
Grading System:
92 – 100 =
A
83 – 91 =
B
75 – 82 =
C
0 – 74 =
F
Students should refer to the Student Handbook for information on Academic Standing Policy,
Academic Honesty Policy, Student Rights and Responsibilities, and other policies relevant to their
academic progress.
Core Curriculum Competencies: (The competencies every graduate will develop)
1.
2.
3.
4.
5.
Communicate clearly and effectively both orally and in writing.
Demonstrate effective problem solving and reasoning skills.
Work effectively in groups of people from diverse backgrounds.
Demonstrate ethical and professional understanding and conduct.
Apply appropriate information literacy skills to locate, evaluate, and use information
effectively.
6. Use computer technology appropriate to the field.
7. Use scientific and mathematical reasoning appropriate to the technology.
Program Graduate Competencies: (The competencies every graduate will develop specific to
his/her major)
1.
2.
3.
4.
5.
Receive and accession tissue specimens accurately.
Prepare tissue specimens for microscopic examinations, including all routine procedures.
Assist with frozen section procedures in histopathology.
Identify tissue structures and their staining characteristics.
Perform preventive and corrective maintenance of equipment and instruments or refer to
appropriate sources for repairs.
6. Explain factors that affect procedures and results, and take appropriate action within
predetermined limits when corrections are indicated.
7. Perform and monitor quality control within predetermined limits.
8. Apply principles of safety to all clinical laboratory procedures.
9. Demonstrate professional conduct and interpersonal communications skills with patients,
the public, laboratory and other health care personnel.
10. Describe the responsibilities of other laboratory and health care personnel and interact
with them with respect for their jobs and patient care.
11. Explain and act upon individual needs for continuing education as a function of growth
and maintenance of professional competence.
12. Exercise principles of management, safety and supervision within the clinical laboratory
environment.