DNA Electrophoresis Gene Human Genetic Genetic Therapy Genome Engineering Disorders and Cloning Project Misc. . Alex Trebek $100 $100 $100 $100 $100 $100 $200 $200 $200 $200 $200 $200 $300 $300 $300 $300 $300 $300 $400 $400 $400 $400 $400 $400 $500 $500 $500 $500 $500 $500 Genetics Part 2 Review Name of the tool used to run a DNA electrophoresis What is agarose gel? What causes the DNA fragments to move What is electrical current? How the DNA fragments are divided once the gel is finished running What is by size (small travel further)? Deer blood was found in the forest after hunting season ended. DNA from this blood was compared to meat and blood found in the suspect’s freezer. Was the suspect guilty? Why or why not? Yes. The DNA fingerprints from the suspect’s freezer match those found in the forest. According to the DNA gel electrophoresis to the right, which is the least likely biological offspring of the couple being tested? What is Child 2? Also known as Trisomy 21 What is Down Syndrome? A human genetic disorder with a dominant inheritance pattern What is Huntington’s disease? The name of the genetic disorder found in the karyotype below What is Turner syndrome? Using the pedigree for Duchenne’s Muscular Dystrophy below, if individual IV-1 had a daughter and a son, what is the probability that the daughter is a carrier and that the son inherited the disorder? 100% chance that the daughter will be a carrier (father’s X has recessive allele). 0% chance that the son will inherit the disorder because the mother is not a carrier. The inheritance pattern shown in the pedigree below What is simple recessive? The thing that transplant DNA is placed into to be delivered to the new host What is a vector? The purpose of gene therapy What is to cure genetic diseases? An exact genetic replicate What is the product of cloning? The name of the cell once the host DNA is removed What is an enucleated egg? A field of research that is currently using stem cell technology What is regenerative medicine? The DNA of one organism is experimentally mixed in with the DNA of another organism What is a transgenic organism? Anti-pest corn is an example of this What is a genetically modified organism (GMO)? The molecule that is used to cut DNA What is a restriction enzyme? Which restriction enzyme in the chart to the left could be used to cut the DNA strand below? What is BamHI? Using the images below, what is the correct order of the steps of the cloning procedure? What is 4, 2, 1, 3? The goal of the Human Genome Project What is to identify all human genes and sequence all the DNA bases? The year the Human Genome Project was completed What is 2003? The organizations that led the Human Genome Project What are the U.S. Dept of Energy and the National Institutes of Health? 3 non-human organisms whose genomes were sequenced leading up to the Human Genome Project What are Escherichia coli bacteria, fruit fly, and mouse? The significance of the goal end-date for the HGP What is the 50th anniversary of the description of the double helix by Watson and Crick? An undifferentiated cell What is a stem cell? All the DNA in an organism What is a genome? Fill in the blanks in the table below Human Blood Types Genotype Antigens Antibodies Phenotype Produced A B AB O Human Blood Types Genotype Antigens Antibodies Phenotype Produced IAIA or IAi A B A IBIB or IBi B A B IAIB A and B A and B AB ii none none O How long the Human Genome Project took to complete What is 13 years? What a restriction enzyme looks for to know where to cut, and what forms when DNA is spliced What is a palindrome pattern and sticky or blunt ends (depending on the enzyme)? Using a Punnett Square answer the following question. A colorblind man marries a woman who is not colorblind. What are the possible genotypes and phenotypes of their children? There are 2 genotype possibilities for the mother. Xr Y XR XR Xr XR Y XR XR Xr X R Y 100% not colorblind (50% carrier females XRXr, 50% normal males XRY) Xr Y XR XR Xr XR Y Xr Xr Xr X R Y 25% colorblind (XrXr) and 75% normal (25% carrier females XRXr, 50% normal males XRY)