Introduction • There are huge differences between the forms of life but cells are the basic unit of life and have many characteristics in common Introduction • Cells are often divided into certain groups based on major characteristics. – Two main groups: • Prokaryotic cells • Eukaryotic cells 2.2 Prokaryotic Cells Prokaryotic Cells • Much smaller and simpler than eukaryotic cells – Most are less than 1 um Prokaryotic Cells • Features of prokaryotic cells – Cell wall – Plasma membrane – Flagella – Riboome – Nucleoid (region containing free DNA) • Be Sure you can Identify all of these!!! 2.2.1 2.2.2 Prokaryotic Cell Prokaryotic Cell Prokaryotic Cells • Cell Wall and Plasma Membrane – Protects and maintains the shape of the cell • Composed of a carbohydrate-protein complex called peptidoglycan – Some bacteria have an additional layer of a type of polysaccharide out the cell wall • This layer makes it possible for some bacteria to adhere to structures such as teeth, skin, and food Prokaryotic Cells • Cell Wall and Plasma Membrane – The plasma membrane is just inside the cell wall and has a composition similar to the membranes of eukaryotic cells • Controls the movement of materials in and out of cells • Cytoplasm occupies the complete interior of the cell Prokaryotic Cells • Pili and flagella – Some bacterial cells contain hair-like growths on the outside of the cell wall • These are pili and are used for attachment • However their main function is in joining bacterial cells in preparation for the transfer of DNA from one cell to another (sexual reproduction) – If bacterium has flagella (plural) or flagellum (singular), they are longer than pili • Allows cell motility Prokaryotic Cells • Ribosomes – Ribosomes occur in all prokaryotic cells and function as sites of protein synthesis • The more protein produced the larger the number of ribosomes. Prokaryotic Cells • The nucleoid region – Is non-compartmentalized and contains a single, long, continuous, circular thread of DNA – In addition to the bacterial chromosome, bacteria may also contain plasmids • These small, circular, DNA molecules are not connected to the main bacterial chromosome. • Replicates independently of the chromosomal DNA • It is not required by the cell under normal conditions but it may help the cell adapt to unusual circumstances Binary Fission • Prokaryotic cells divide by a very simple process called binary fission. – During this process: • DNA is copied • The two daughter chromosomes become attached to different regions on the plasma membrane • The cell divides into two genetically identical daughter cells Prokaryotic Cells divide by binary 2.2.4 fission 2.3 Eukaryotic Cells The Parts of the Cell • Each living cell carries out the tasks of taking food, transforming food into energy, getting rid of wastes, and reproducing. • Most eukaryotic cells have three main components: – Cell Membrane – Cytoskeleton – Nucleus Animal Cell 2.3.1 2.3.2 Now let Annotate this picture with all the functions of each structure named Plant Cell Now let Annotate this picture with all the functions of each structure named Liver Cell 2.3.3 Structure and Function of Organelles • The Structure and Function of the following organelles will be discussed: – – – – – – – – Cell Membrane Nucleus Cell Wall Cytoplasm Cytoskeleton Ribosomes Endoplasmic Reticulum Golgi Apparatus – – – – – – – – Mitochondria Lysosomes Peroxisomes Cilia and Flagella Basal Bodies Centrioles Vacuoles Plastids Cell Membrane • Structure: phospholipid bilayer with proteins that function as channels, markers, and receptors -also contains cholesterol which provides rigidity • Function: selectively permeable boundary between the cell and the external environment Nucleus • Structure: the nucleus is a sphere that contains another sphere called a nucleolus • Function: -storage center of cell’s DNA -manages cell functions Cell Wall • Structure: rigid wall made up of cellulose, proteins, and carbohydrates • Function: boundary around the plant cell outside of the cell membrane that provides structure and support Cytoplasm • Structure: gelatin-like fluid that lies inside the cell membrane • Function: -contains salts, minerals and organic molecules -surrounds the organelles Cytoskeleton • Structure: a network of thin, fibrous elements made up of microtubules (hollow tubes) and microfilaments (threads made out of actin) • Function: -acts as a support system for organelles -maintains cell shape Ribosomes • Structure: consist of two subunits made of protein and RNA • Function: location of protein synthesis Endoplasmic Reticulum • Structure: a system of membranous tubules and sacs • Function: intercellular highway (a path along which molecules move from one part of the cell to another) • Two types: – Rough Endoplasmic Reticulum – Smooth Endoplasmic Reticulum Rough Endoplasmic Reticulum • Rough Endoplasmic Reticulum (rER): prominent in cells that make large amounts of proteins to be exported from the cell or inserted into the cell membrane – Covered with ribosomes Smooth Endoplasmic Reticulum • Smooth Endoplasmic Reticulum (sER): involved in the synthesis of lipids and breakdown of toxic substances – Not covered with ribosomes Golgi Apparatus • Structure: stacked flat sacs • Function: receives proteins from the rER and distributes them to other organelles or out of the cell (receiving, processing, packaging, and shipping) Mitochondria • Structure: folded membrane within an outer membrane – The folds of the inner membrane are called cristae • Function: -converts energy stored in food into usable energy for work – cellular respiration Lysosomes • Structure: spherical organelles that contain hydrolytic enzymes within single membranes • Function: breaks down food particles, invading objects, or worn out cell parts Peroxisomes • Structure: spherical organelles that contain enzymes within single membranes • Function: Degrade hydrogen peroxide, a toxic compound that can be produced during metabolism. Cilia and Flagella • Structure: hair-like organelles that extend from the surface of cells – When they are present in large numbers on a cell they are called cilia – When they are less numerous and longer they are called flagella – Both organelles are composed of nine pairs of microtubules arranged around a central pair. • Function: cell motility Cillia and Flagella Basal Bodies • The microtubule assembly of a cilium or flagellum is anchored in the cell by a basal body. • Structurally identical to a centriole Centrioles • Structure: composed of nine sets of triplet microtubules arranged in a ring – Exist in pairs • Function: centrioles play a major role in cell division (mitosis) Vacuoles • Structure: a sac of fluid surrounded by a membrane – Very large in plants • Function: used for temporary storage of wastes, nutrients, and water Plastids • There are three types of plastids in plant cells: – Chloroplasts (discussed on next slide) – Chromoplasts: synthesize and store pigments – Leucoplasts: store food such as starches, proteins, and lipids Chromoplasts Red Pepper Flower Leucoplasts Chloroplasts • Structure: stacked sacs (thylakoids) that contain chlorophyll surrounded by a double membrane • Function: photosynthesis (conversion of light energy to chemical energy stored in the bonds of glucose) Structural Organization of Eukaryotic and Prokaryotic Cells 2.3.4 2.3.4 Eukaryotes vs. Prokaryotes • Eukaryotes (animals, plants, fungi, protists) and prokaryotes (bacteria) differ greatly in structure. Prokaryotic cells Eukaryotic cells DNA in a ring form without protein DNA with proteins as chromosomes/chromatin DNA free in cytoplasm DNA enclosed within a nuclear envelope No mitochondria Mitochondria present 70S ribosome 80S ribosome Not internal compartmentalization to form organelles Internal compartmentalization present to form many types of organelle Size less than 10 micrometres Size more than 10 micrometres • Similarities between two types of cells: – Both types of cells have some sort of outside boundary that always involves a plasma membrane – Both types of cell carry out all the functions of life – DNA is present in both cell types Plant cells Animal Cells Exterior of cell includes an outer cell wall with a plasma membrane just inside Exterior of cell includes only a plasma membrane. There is no cell wall Chloroplasts are present in the cytoplasm There are no chloroplasts Possess large centrally located vacuoles Vacuoles are usually not present or are small Store carbohydrates as starch Store carbohydrates as glycogen Do not contain centrioles within a centrosome area Contain centrioles within a centrosome area Because a rigid cell wall is present, this cell type has a fixed, often angular, shape Without a cell wall, this cell is flexible and more likely to be rounded shape 2.3.5 Plant Cells vs. Animal Cells • Animal cells are very similar to plant cells except for the following major differences: – Animal cells do not contain chloroplasts – Animal cells are not surrounded by cell walls – The vacuoles in plants are much larger than those of animals Microscope Pictures of a Plant Cell and an Animal Cell Elodea Human Cheek Cells Extracellular components • The extracellular matrix (ECM) is composed of collegen fibers plus a combination of sugars and proteins called glycoproteins in many animal cells – These form fiber-like structures that anchor the matrix to the plasma membrane. This strengthens the plasma membrane and allows attachment between adjacent cells. Extracellular components • The ECM allows for cell to cell interaction, possibly altering gene expression and bringing about coordination of cell action within the tissue. – Many researchers think the ECM is involved in directing stem cells to differentiate – Cell migration and movement also appear to be the result of the interactions between cells Extracellular components 2.3.6 THE END!