Chapter 17 1 VOCABULARY Aerobic respiration Fermentation Antibiotic Motility Antiseptic Mutation Bacterial culture Prokaryote Binary fission Resistant/resistance Conjugation Classification Disinfectant Ecological role 2 READING Basic structure of prokaryotic cell – p.360-361 Unifying characteristics – p.361-364 Form, distribution, motility, ecological role, nutrition, human diseases – p.361-366, 369-372 Fermentation, aerobic respiration, photosynthesis in Monerans – p.365-367 How bacterial decomposers and parasites obtain their food – p.365 Antibiotics, disinfectants, antiseptics – p.374 Antibiotic resistance – p.368 Beneficial roles – p370-372 3 THE FIVE KINGDOMS 4 Introduction to Bacteria 2 TYPES OF BACTERIA: •Bacteria -Get food from an outside source •Blue-green Bacteria -Make their own food 5 BACTERIA Bacteria - small one celled monerans Bacteria like a warm, dark, and moist environment They are found almost everywhere: -water -soil -skin -air -food -inside the body -on most objects 6 3 Shapes of Bacteria Bacteria are classified by shape into 3 groups: Spiral: spirilla rod-shaped: bacilli, bacillus Round: cocci 7 3 Shapes of Bacteria Bacillus anthracis – (bacillus) Neisseria meningitidis (coccus) Leptospira interrogans – (spirilla) 8 7 Major Structures of a Bacteria Cell •Capsule •Cell wall •Ribosomes •Nucleoid •Flagella •Pilli •Cytoplasm 9 7 Major Structures of a Bacteria Cell Capsule keeps the cell from drying out and helps it stick to food or other cells 10 7 Major Structures of a Bacteria Cell Cell wall Thick outer covering that maintains the overall shape of the bacterial cell 11 7 Major Structures of a Bacteria Cell Ribosomes cell part where proteins are made Ribosomes give the cytoplasm of bacteria a granular appearance in electron micrographs 12 7 Major Structures of a Bacteria Cell Nucleoid a ring made up of DNA 13 7 Major Structures of a Bacteria Cell Flagella a whip-like tail that some bacteria have for locomotion 14 7 Major Structures of a Bacteria Cell Pilli hollow hair-like structures made of protein allows bacteria to attach to other cells. Pilli-singular Pillus-plural 15 7 Major Structures of a Bacteria Cell Cytoplasm clear jelly-like material that makes up most of the cell 16 Bacteria Reproduce Quickly In optimal conditions, an E. coli bacteria can double every 20 minutes. The time it takes a bacteria to double is known as the Generation time A Growth Table Time (min) 0 20 40 # of E. Coli 1 2 4 Time (min) 200 220 240 # of E. Coli 1024 2048 4096 60 80 100 8 16 32 260 280 300 8192 16384 32768 120 64 320 65536 140 160 128 256 340 360 131072 262144 180 512 380 524288 At just over 6.5 hours you have over 1 MILLION E. coli. Imagine that growing on your sandwich This type of growth has a special name. It is known as Exponential growth. Exponential growth starts out slow but once it gets going it grows quickly. Lets Graph it! # of E. coli 1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 Time # of E. coli versus Time 140000 120000 80000 60000 40000 20000 Time (min) 340 320 300 280 260 240 220 200 180 160 140 120 100 80 60 40 20 0 0 # of E. coli 100000 Salmonella Salmonella has a generation time of about 30 minutes It can be found on chicken and can make you quite sick. Luckily it takes about 10 million of them to get sick so that’s quite a few. Lets calculate how long it takes for bacteria to reach 10,000,000 if we have 1000 on our chicken sandwich. The Special Equation There is a special equation to use with exponential growth N = N02t/T N = is the number of bacteria N0 = the number of bacteria you start with T = the amount of time it takes to double t = the actual time In ideal optimal conditions in about 3 days bacteria could reproduce sooooo much that they would weigh more than our planet. Lucky for us Lucky for us those conditions don’t usually exist, and a graph of bacterial growth generally looks like this Reproduction of Bacteria •Binary Fission- the process of one organism dividing into two organisms •Fission is a type of asexual reproduction •Asexual reproduction- reproduction of a living thing from only one parent How?... The one main (circular) chromosome makes a copy of itself Then it divides into two 29 Reproduction of Bacteria BINARY FISSION Bacteria dividing Completed 30 Reproduction of Bacteria •The time of reproduction depends on how desirable the conditions are •Bacteria can rapidly reproduce themselves in warm, dark, and moist conditions •Some can reproduce every 20 minutes (one bacteria could be an ancestor to one million bacteria in six hours) 31 Bacterial Cell & Nucleiod DNA Ring DNA replication Cell wall synthesis Cell separation 32 Bacteria Survival Endospore•a thick celled structure that forms inside the cell •they are the major cause of food poisoning •allows the bacteria to survive for many years •they can withstand boiling, freezing, and extremely dry conditions •it encloses all the nuclear materials and some cytoplasm 33 Bacteria Survival Bacillus subtilis Endospore-the black section in the middle highly resistant structures can withstand radiation, UV light, and boiling at 120oC for 15 minutes. 34 Bacteria Survival – Food sources parasites – bacteria that feed on living things saprophytes – use dead materials for food (exclusively) decomposers – get food from breaking down dead matter into simple chemicals important- because they send minerals and other materials back into the soil so other organisms can use them 35 Harmful Bacteria • some bacteria cause diseases •Animals can pass diseases to humans Communicable Disease – Disease passed from one organism to another This can happen in several ways: •Air •Touching clothing, food, silverware, or toothbrush •Drinking water that contains bacteria 36 Harmful Bacteria Human tooth with accumulation of bacterial plaque (smooth areas) and calcified tartar (rough areas) 37 Helpful Bacteria •Decomposers help recycle nutrients into the soil for other organisms to grow •Bacteria grow in the stomach of a cow to break down grass and hay •Most are used to make antibiotics •Some bacteria help make insulin •Used to make industrial chemicals 38 Helpful Bacteria E.coli on small intestines 39 Helpful Bacteria •Used to treat sewage Organic waste is consumed by the bacteria, used as nutrients by the bacteria, and is no longer present to produce odors, sludge, pollution, or unsightly mess. •foods like yogurt, cottage & Swiss cheese, sour cream, buttermilk are made from bacteria that grows in milk 40 Controlling Bacteria 3 ways to control bacteria: 1) Canning- the process of sealing food in airtight cans or jars after killing bacteria •endospores are killed during this process 2) Pasteurization- process of heating milk to kill harmful bacteria 3) Dehydration- removing water from food •Bacteria can’t grow when H2O is removed •example: uncooked noodles & cold cereal 41 Controlling Bacteria Antiseptic vs. Disinfectants Antiseptic- chemicals that kill bacteria on living things •means – “against infection” Examples: iodine, hydrogen peroxide, alcohol, soap, mouthwash Disinfectants- stronger chemicals that destroy bacteria on objects or nonliving things 42 BLUE-GREEN BACTERIA Autotrophs – make their own food through photosynthesis larger than most bacterial cells commonly grow on water and surfaces that stay wet…such as rivers, creeks and dams Some live in salt water, snow, and acid water of hot springs food source for animals that live in the water 43 BLUE-GREEN BACTERIA can be toxic to humans and animals Blooms- occur when the bacteria multiplies in great numbers and form scum on the top of the water 44 45 Bacillus anthracis - rod, vegetative stage prokaryote (bacterium) Image Number: 21185A 46 Neisseria meningitidis - coccus prokaryote (bacterium) Image Number: 97214E 47 Leptospira interrogans spiral shaped prokaryote (spirochete) 48