Unknown Bacteria
by
Jon Samel
Or what the heck is that growing on Dr.
Crawford’s laptop?
• A swab was taken from Dr. Crawford’s laptop keyboard.
• Growth on the plate showed two bacilli.
• Successive attempts to separate the bacteria proved
unsuccessful.
Unknown Bacteria
• Sample was pale white, round and convex.
• Culture one was grown on Triptic Soy Agar at a
temperature of 37 deg. Celsius.
• Incubation time was 72 hours.
Unknown Bacteria
• Two bacteria located in the sample. One identified as
Staphylobacillus by Dr. Crawford.
• Initial simple stains with Methylene Blue did not stain
plasma membrane but did stain the organelles.
• Staining with Crystal Violet revealed single rods and
clusters.
Unknown Bacteria
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Gram stain positive.
Capsule stain negative.
Acid fast positive. (Note results questionable)
Catalase test positive.
Endospore test shows sub terminal endospores.
Unknown Bacteria
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Starch test positive.
Anaerobic test shows facultative anaerobic growth.
Phenol red test shows pH but no gas formation.
VP test A – Methyl Red positive. (Mixed acid)
VP test B – Negative
Unknown Bacteria
• Mannitol Salt Agar shows --------• Four further attempts to isolate a pure culture proved
unsuccessful.
• Cultures were repeated in successively lower
temperatures. *37, 34, 32 and 20 deg. Celsius*
Unknown Bacteria
• Final conclusion is that the unknown bacteria is a
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• This bacteria is known for ---------------
Unknown Bacteria
References
Unknown Bacteria