PARASITOLOGY Prepared by : Reem Aldossari General characteristics The Protozoa are the simplest and most primitive animals. They live either singly or in colonies. Some are parasitic. They are usually defined as "unicellular" animals. Amoeba • Habitat: freshwater ponds. • Means of locomotion: pseudopodia. • Reproduction mechanism: asexually by binary fission, and under unfavourable conditions it encysts. • Shape and characteristics: quite irregular in shape; the body changes its shape constantly with the formation and withdrawal of the pseudopodia. Kingdom :Protista Phylum: Sarcomastigophora Subphylum: Sarcodina Genus : Amoeba Species :Amoeba nucleus Contractile vacuole Food vacuoles Ectoplasm pseudopodia Endoplasm Entamoeba histolytica •Entamoeba histolytica: causes amoebic dysentery. Life cycle : •The trophozoite: feedingdividing form) It has one pseudopodia). •Cyst: have 4 nuclei (infective stages( •Infective stage: Cyst. •Mode of infection: contamination of food and water with cyst. •Characteristic features: Cyst have 4 nuclei. Trophozoite have RBCs in Kingdom :Protista Phylum: Sarcomastigophora Subphylum: Sarcodina Genus: Endamoeba Species: Endamoeba histolytica Entamoeba coli •Endamoeba coli: does not cause any known disease •Habitat: large intestine of human . •Characteristics: mature cysts have 8 nuclei Kingdom :Protista Phylum: Sarcomastigophora Subphylum: Sarcodina Genus: Endamoeba Species: Endamoeba coli Euglena • Habitat: ponds and stagnant water. • Characteristics: Green in colour (have chloroplasts) • Means of locomotion: flagellum • Reproduction: asexually by longitudinal binary fission and is able to form a cyst. Kingdom :Protista Phylum : Sarcomastigophora Subphylum : Mastigophora Class : Phytomastigophora Genus : Euglena Flagellum Eyespot Second flagellum Nucleus Chloroplast Means of locomotion: cilia Paramecium Habitat: freshwater ponds where decaying organic matter is abundant, feeding on it and on bacteria and other microorganisms. Reproduction: asexually by transverse binary fission, and sexually by conjugation . Characteristics: the numerous cilia covering the whole surface of the body. They have two contractile vacuoles and two nuclei: a large oval macronucleus (for vegetative functions), and a Kingdom : Protista Phylum: Ciliophora Genus: Paramecium Intermediate host: insect or leechTrypanosoma Habitat in intermediate host: alimentary canal Definite host: human. Habitat in definite host: blood of man Causes the disease: sleeping sickness. Means of locomotion: flagellum. Feeding habits: absorbing the nutrient substances from the host. Reproduction: reproduce asexually by longitudinal binary fission. Major characteristics: long Kingdom : Protista Phylum: Sarcomastigophora Subphylum: Mastigophora Class: Zoomastigophora Genus: Trypanosoma Species: gambiense Trypanosoma gambiense Plasmodium malariae Trophozoite: habitat: live intracellularly in the blood corpuscles of its vertebrate host. Causes the disease: malaria fever to man. Vector: a mosquito belonging to the genus Anopheles. Kingdom: Protista Phylum: Apicomplexa Class: Sporozoa Genus: plasmodium Species: Plasmodium malaria Life cycle: •Ring stage •Trophozoite •Merzoites stage •Release of merozoites Macrogametocyte Release of merozoites Formation of Merozoites Microgametocyte Ring stage Trophzoite stage 2 3 1 (Amoeba) األميبا Nucleus نواة-1 Pseudopodia أرجل كاذبة-2 Food vacuole فجوة غذائية-3 1 2 3 حوصلة انتاميبا كوالي Entamoeba coli -1جدار الحويصلة Cyst Wall )8-4( -2أنوية 4-8 Nuclei -3سيتوبالزم Cytoplasm 1 2 3 حوصلة انتاميبا هستوليتكا Entamoeba histolytica -1جدار الحويصلة Cyst Wall )4-1( -2أنوية 1-4 Nuclei -3سيتوبالزم Cytoplasm اعداد األستاذة /أفراح الخريجي 1 3 2 (Euglena) اليوجلينا Flagellum Contractile vacuole Nucleus السوط-1 فجوة متقبضة-2 نواة-3 1 2 3 4 (Trypanosoma) التريبانوسوما Red blood cell Flagellum Undulating membrane Nucleus كريات دم حمراء-1 السوط-2 غشاء متموج-3 نواة-4 1 2 البالزموديوم Plasmodium -1الطور الحلقي -2كريات دم حمراء Ring Stage Red blood cell 1 2 البالزموديوم Plasmodium -1الطور األميبي -2كريات دم حمراء Amoeboid stage Red blood cell 1 2 Plasmodium البالزموديوم Schizonte(formation of merozoites) ) الشيزونت( تكوين الميروزيتات-1 Red blood cell كريات دم حمراء-2 1 2 Plasmodium البالزموديوم Release of merozoites Red blood cell إنطالق الميروزيتات-1 كريات دم حمراء-2 1 4 2 3 4 (Paramecium) البراميسيوم Cilia macronucleus micronucleus Contractile vacuole أهداب-1 نواة كبيرة-2 نواة صغيرة-3 فجوة متقبضة-4 Thank you for attention