ALL-RUSSIAN PLANT QUARANTINE CENTER FGU VNIIKR EUPHRESCO II Copenhagen 24-25 March 2011 N. Sherokolava MINISTRY OF AGRICULTURE OF THE RUSSIAN FEDERATION FEDERAL SERVICE FOR VETERINARY AND PHYTOSANITARY SURVEILLANCE (ROSSELKHOZNADZOR) (National Plant Protection Organization) DIRECTORATE FOR PHYTOSANITARY SURVEILLANCE AND GRAIN QUALITY AND SAFETY CONTROL ROSSELKHOZNADZOR REGIONAL OFFICES (64 DIRECTORATES) ALL-RUSSIAN PLANT QUARANTINE CENTER (FGU VNIIKR) FGU VNIIKR BRANCHES (24 BRANCHES) ACCREDITED (REFERENCE) CENTERS (27 CENTERS) VNIIKR’S CORE ACTIVITIES PROVIDING SCIENTIFIC AND METHODOLOGICAL BACKGROUND FOR NPPO’S ACTIVITIES PLANT QUARANTINE DIAGNOSTIC LABORATORY SYNTHESIS AND FIELD TRIAL OF QUARANTINE INSECT PHEROMONES PARTICIPATING IN DETECTION SURVEYS OF RUSSIA’S TERRITORIES DESINFESTATION OF REGULATED ARTICLES AND MATERIALS REFRESHER TRAINING FOR NPPO’S SPECIALISTS RUNNING PHYTOSANITARY PROGRAMMES SCIENTIFIC AND METHODOLOGICAL BACKGROUND FOR NPPO’S ACTIVITIES PRAs for Pests of Potential Quarantine Importance Development of Containment and Eradication Methods for Managing Quarantine Pest Outbreaks: BIOLOGICAL CONTROL METHOD FOR THE POTATO COLORADO BEETLE LEPTINOTARSA DECEMLINEATA SAY. AND THE TWENTYEIGHT SPOTTED LADYBIRD EPILACHNA VIGINTIOCTOMACULATA MOTSCH. USING THE ENTOMOPHAGOUS PREDATORY SHIELDBUG PICROMERUS BIDENS L. RUNNING PHYTOSANITARY PROGRAMMES SCIENTIFIC AND METHODOLOGICAL BACKGROUND FOR NPPO’S ACTIVITIES Studies on Factors Causing Pine Wilt Disease Studies on Phytosanitary Peculiarities Related to the Biology of Quarantine Pests: THE ABILITY OF QUARANTINE DODDER PLANTS OF CUSCUTA SPP. TO PRESERVE AND SPREAD QUARANTINE PLANT PATHOGENS FORMING A COLLECTION OF INSECTS (PRIMARILY, COLEOPTERA AND LEPIDOPTERA SPP.) THROUGH STUDYING RUSSIA’S BIODIVERSITY RUNNING PHYTOSANITARY PROGRAMMES PLANT QUARANTINE DIAGNOSTIC LABORATORY IMPROVEMENT OF METHODS FOR DETECTION AND IDENTIFICATION OF QUARANTINE PESTS: • Genetic and Serological Diversity and Biochemical Characteristics of the Russian Strains of Erwinia amylovora. Optimization of Growth Medium for Enrichment of Erwinia amylovora in a Sample. • Optimization of Diagnostic Methods for Xanthomonas oryzae, Search for Genetic Markers to Develop Diagnostic Kits for Differentiation of Bacterial Rice Blight from Closely Related Xanthomonas spp. • Development of Diagnostic Methods for Phytophthora fragarie Using Conventional and Real-Time PCR and Direct Sequencing. Developing a Diagnostic Method Combining Biobaits and Molecular Methods. RUNNING PHYTOSANITARY PROGRAMMES PLANT QUARANTINE DIAGNOSTIC LABORATORY IMPROVEMENT OF METHODS FOR DETECTION AND IDENTIFICATION OF QUARANTINE PESTS: • Development and Improvement of Molecular Diagnostic Methods for Potato Viruses: APMoV (Andean Potato Mottle Virus), APLV (Andean Potato Latent Virus) and PYDV (Potato Yellow Dwarf Virus). • Development and Improvement of Molecular Diagnostic Methods for Potentially Hazardous Viruses: Tomato Ringspot Virus, Tobacco Ringspot Virus, Beet Necrotic Yellow Vein Virus. •Studies on the PPV (Plum Pox Virus) Strain Diversity in the Russian Population of the Pathogen and Development of a Multiplex Diagnostic Method with Real-Time PCR and PPV Strain Identification. RUNNING PHYTOSANITARY PROGRAMMES SYNTHESIS OF INSECT PHEROMONES IMPROVEMENT OF METHODS FOR QUARANTINE PEST DETECTION AND OUTBREAK CONTAINMENT AND ERADICATION USING PHEROMONES: •National Programme for Detection of Quarantine Pests Using Pheromone and Coloured Traps in the Areas of the Highest Phytosanitary Risk: 2011 - 2015. • Studies on Pheromones of the Following Pests, Their Synthesis and Field Trials: Carposina niponensis; Ceratitis capitata; Diabrotica virgifera virgifera; Phthorimaea operculella; Trogoderma granarium; Quadraspidiotus perniciosus; Pseudaulacaspis pentagona; Hyphantria cunea; Tuta absoluta. RUNNING PHYTOSANITARY PROGRAMMES DETECTION SURVEYS Phytosanitary Surveys: •Phytosanitary Monitoring of Grain Crops Growing on the Territory of the Russian Federation •Phytosanitary Monitoring of Potato Crops for Detection of Quarantine Pests •Phytosanitary Monitoring of Fire Blight Distribution on the Territory of the Russian Federation RUNNING PHYTOSANITARY PROGRAMMES DESINFESTATION Trials of Methyl Ioidide for Quarantine Desinfestation against Orchard and Stored Product Pests Expanding the Use of Phosphine Preparations, Alternative to Methyl Bromide; Development of Schedules and Techniques for Application of These Preparations To Desinfest Fresh Fruits, Plants for Planting and Wood Materials Trials of Sulfuryl Fluoride for Control of Stored Product Pests, Forest Pests and Pests of Wood Products PLANNED THEMES FOR PHYTOSANITARY RESEARCH Studies on Communicative Peculiarities of Picromerus bidens for Optimizing the Rearing and Use of the Entomophage Development of Phytosanitary Demarcation Methods to Be Used on the Territory of the Russian Federation PARTNTERS Research Institutions: Russian State Agrarian University – K.A. Timiryazev MTAA Bioengineering Centre of the Russian Academy of Sciences All-Russian Research Institute for Phytopathology A.G.Lorkha Russian Potato Research Institute Zoological Institute of the Russian Academy of Sciences (St. Petersburg) A.N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences St. Petersburg State Forest-Technical Academy Krasnodar Research Institute for Biological Protection of Plants Plant Protection Institute, Bulgaria Nanjing Forestry University, China Geneva Natural History Museum, Switzerland Commercial Firms: ZAO Syntol Agrodiagnostics Ltd. THANK YOU FOR YOUR ATTENTION!