LABORATORY CONDUCT
AND
SAFETY REGULATIONS
(Indoor & Field Safety)
Biology
Lake City High School
Mrs. Whittington
Standard
Standard B-1 The student will demonstrate an
understanding of how scientific inquiry and
technological design, including mathematical
analysis, can be used appropriately to pose questions,
seek answers, and develop solutions.
• B-1.9
• Use appropriate safety procedures
when conducting investigations.
Safety regulations are established for
each laboratory by Federal, State, and
School mandates.
Objectives
Successful work in the laboratory and field
involves not only mastery of concepts and
techniques, but also knowing:
• what the safety requirements are,
• what equipment is available, and
• when and how to use each piece of equipment
• how to keep yourself and others safe and
• how to respond to emergencies.
Safety regulations are established for
each laboratory by Federal, State, and
School mandates.
Universal precautions
and basic lab safety
rules will be strictly
enforced
NON-COMPLIANCE
• 1st violation: leave the activity
area until compliant
–student may receive a zero for
work missed
• 2nd violation: leave the activity
area for the day
–student may receive a zero for
all work missed that day
NON-COMPLIANCE
• 3rd violation: leave the activity
area permanently
–student may receive a failing
lab grade
• *Severe violations will warrant
immediate removal from the lab
–student may receive a failing
lab grade
Lab Safety
STUDENT BEHAVIOR
• No horseplay (pushing,
shoving, etc...) in the lab
• Always be aware of
potential hazards & act
cautiously in the lab
Read, read, read
• BEFORE beginning a
lab READ ALL safety
precautions and lab
instructions..
Eating & Drinking
• Food and drink of any
kind are strictly
prohibited in the lab.
• Report all medical conditions
(pregnancy, cardiac or respiratory
disorders, allergies....)
• Only authorized people in
the lab.
• All human secretions will be treated
as biohazards
Stow books, backpacks,
purses, etc... under the desk
• Lab aprons (tied in back)
• Long hair must be tied back
• Only closed toed shoes
APPAREL
Proper Clothing
Tie back
loose hair!
Avoid floppy
clothing!
Store
belongings
out of the
way!
Never take any lab
materials out of the lab.
• Lab materials are costly and
may be dangerous as well.
• Taking them may result in
school discipline or even
legal prosecution.
Eye and Face Safety
• No contact lenses
during wet labs (when
using chemicals or
biohazards)
• Safety glasses during
all wet labs
• Disposable gloves
when working with
hazards or chemicals
Eye Protection
O
Z87 Type Goggles
NO Food, Drink, Tobacco,
Cosmetic use
Biology I Bell Ringer
• You notice in the
lab instructions that
you will be using
test tubes. What
are two rules that
you know you will
need to remember?
(Hint: There may be more
than 2, but you only need
to write about two.)
Bell Ringer
Free Write Friday
• Write at least 3
sentences on
whatever you
like.
• Examples: Tell
me how your
first week at
school has been.
Have any plans
for the
weekend?
Be prepared to turn in
your bell ringers.
Today
• Reminder Safety Contracts due today.
– You may turn it in late for a point reduction.
• Anyone miss yesterday’s pre-test?
– Everyone must take the pre-test
• Safety Quiz – Postponed due to printer
issues. Will be on Tuesday!
• Put standards page in your folder. Initial the
safety indicator (B 1.9)
• 1st Period – Acceptable Use Policy!
Assignment
• You will be assigned a rule from the safety
contract.
• Your mission is to create a sign that will
remind people to follow that rule.
• Put your first and last name and the class
period in the upper right hand corner.
– Must communicate the rule
– Must be creative and neat
– Must finish in class
Standard
Standard B-1 The student will demonstrate an
understanding of how scientific inquiry and
technological design, including mathematical
analysis, can be used appropriately to pose questions,
seek answers, and develop solutions.
• B-1.9
• Use appropriate safety procedures
when conducting investigations.
Safety regulations are established for
each laboratory by Federal, State, and
School mandates.
Objectives
Successful work in the laboratory and field
involves not only mastery of concepts and
techniques, but also knowing:
• what the safety requirements are,
• what equipment is available, and
• when and how to use each piece of equipment
• how to keep yourself and others safe and
• how to respond to emergencies.
Safety regulations are established for
each laboratory by Federal, State, and
School mandates.
Glassware Safety
Special Caution-Handling
Glassware
Protect hands.
Lubricate glass
surfaces.
NEVER force
the glassware!
Special Caution-Handling
Glassware
Check for any
cracks, bubbles, or
rough areas before
using.
Broken Glass Disposal
UNCONTAMINATED broken glass
Fire Safety
• Watch for FLAMES from
burners
• No unauthorized or
unsupervised experiments
• Immediately report ALL
– accidents
– injuries
– spills
FIRE SAFETY
• Fire Extinguishers:
• Aim nozzle at the
base of the fire
• Pull pin and
squeeze handles
together.
Fire Blanket:
• Pull the blanket
out of the bottom
using the black
cord
• carefully cover the person to
smother the fire
Fire Alarm:
• Pull the handle on the fire alarm to
activate.
• Exit the building
• Emergency exit route:
–Exit the lab and building.
–Meet the teacher at the designated
site
–Do NOT leave until you are released
Sharp Instruments
Sharps
Handle with Care!
Sharps Disposal
• Examples:
–Needles
–Razor blades
–Scalpels
–Scalpel blades
INSTRUCTIONS FOR
WASTE CLEANUP
CHEMICAL WASTE
DISPOSAL
Dilution is NOT the
solution to pollution!!!
LAB CLEAN-UP
• Hands must be washed
after removing gloves and
before leaving the lab
• Glassware must be washed with soap
& water & rinsed with distilled water
• Clean & disinfect your work area
BEFORE leaving lab.
• Everything must be returned to its
proper place
WASTE DISPOSAL
• All waste has a specific
disposal container
• Always use the properly
labeled container
• If unsure ask the teacher.
Don’t pour ANYTHING down
the sink unless you are
instructed to do so.
Biohazard Waste Labeling
• LABEL ALL CONTAINTERS
before putting anything in
them with the following:
– Date
– Organism (or substance)
– Medium (or chemical)
– Your initials & class period
• Know what waste products
will be generated.
• Chemical Waste Labeling:
–Obtain a clean, dry, empty
container.
–Place a new CHEMICAL
WASTE label on front.
• DO NOT mix liquid & solid
waste.
• Liquid waste SHOULD NOT be
mixed unless it is of the same
type.
• Fill container just below neck of
the bottle.
Biohazard Disposal
A BIOHAZARD IS ANTYING THAT
HAS COME INTO CONTACT WITH
• BACTERIA
• FUNGI
• VIRUSES
• BODILY FLUIDS
Plastic Biohazard Bag
• Examples:
– Petri dishes
– Specimen
containers
– Pipette tips
– contaminated
gloves
Biohazard Disposal Boxes
• Examples:
Contaminated
– microscope slides
– coverslips
– broken glass
– pipettes
– test tubes
Cardboard Biohazard Box
• Examples:
–Wooden probes
–Toothpicks
Trash Receptacle
UNCONTAMINATED
gloves & paper towels
Chemical Safety
SAFETY CHECKLIST
• MSDS - Materials Safety Data
Sheets chemical facts (ex: health
hazards, first aid, disposal, etc...)
SOP FOR CHEMICAL SPILLS
• Immediately report all spills
• Proper safety apparel must be
worn before clean-up of a spill
– Lab coat
– Goggles
– Gloves
Emergency
Shower:
• Pull the lever
• Stand under water for at least
fifteen (15) minutes.
• Only pull lever if you need it, there
is no drain close by
Eye Wash Station:
• Pop the caps off,
• Place the person's
face over the
fountain,
• Push the handle back Eyes must be
held wide open,
• Flush at least fifteen (15) minutes
SOLID SPILLS
• If solid is inorganic and without heavy
metals, sweep up & put in trash.
• If solid is organic or has heavy metals,
sweep up & put in ziplock baggie.
• Label & take to coordinator for
disposal.
• Sweep up all broken glass & put in
broken glass container.
• First Aid:
–Neutralize the danger if
possible.
–If a student or teacher is
injured, notify the office and
they will send a first
responder.
Mercury Thermometer Breaks
• Mercury thermometers are no
longer used in school labs.
• If a thermometer should break,
notify the teacher so that they
can check to be sure it does not
contain mercury.
Electrical Safety
Electrical Safety
Do not put anything other than an
electrical plug into an outlet.
Keep electrical equipment and
cords away from water or other
liquids.
Check equipment for broken or
frayed cords.
Lab Equipment
• NEVER use or handle lab
equipment or other materials
unless instructed to do so.
Pipetting
NOT
With your
mouth
MICROSCOPES
• Hold with both hands
when transporting
the scope
• Clean after every use
• Store with scanning
objective in position
& stage lowered
Funnel
Metric Ruler
Microscope
Flask
Test Tubes
Balance
Thermometer
Scissors
Scalpel
Forceps
Magnifying Glass
Graduated Cylinder
Beaker
Pipette
Medicine Dropper
Dissecting Tray
Microscope Slide
Cover slip
Dissecting Probes
Petri Dish
• You pour an acid down the
sink, although your teacher did
not tell you to.
Is this permissible?
NO
Should you pour an acid into
water or water into an acid?
Acid into water.
• Should you wear goggles
when using a bunsen
burner?
• YES
• You get HCL on your body,
what should you do?
• Tell Your Teacher.
• Rinse it off with water.
NO
• You know you have salt in a
test tube and you don’t think
heating it will hurt you or
anyone else. Should you heat
the salt even though your
teacher didn’t tell you to?
• There is some salt in a beaker
as part of an experiment you
are doing. You want to make
sure it is the salt, should you
taste it?
• NO
• You are doing a lab with
sugar water. It calls for 10
ml placed in a graduated
cylinder. You goofed and
poured in 15 ml. You
decide to return 5 ml. back
to the beaker of sugar
water. Is this okay?
•Treat all
microorganisms as if
they are harmful even
if they don’t cause
diseases in humans.
True or False?
• What is proper procedure
when done with a lab?
• Clean up work area
• Wash hands
• Put equipment back where
you got it from
Safety Briefing Forms
• Fill out the student information
form - which includes
emergency information.
• Sign the Laboratory Conduct &
Safety Regulations - Student
Agreement.
Nature Trail Safety
1. Be Aware of Your Surroundings
at all time.
2. Look down and up as well as
around you.
3. Watch your step.
Nature Trail Safety
• You must walk with a partner.
• If you get separated from your
partner, return to the bench area.
• Stay within hearing range of other
groups.
Nature Trail Safety
• When looking under logs or other
materials, turn the object so that anything
under it can escape AWAY from you.
• Do not try to roll over large objects.
• Do not handle spiders, unfamiliar insects,
lizards, or animals without supervision.
Nature Trail Safety
• Never put your hands or feet in areas that
you cannot see clearly (piles of leaves,
holes, under trees, etc.)
• Use a long branch, walking stick, or other
tool to clear away deep leaves or turn over
small logs.
Nature Trail Safety
• You must wear appropriate
clothing. Sturdy shoes (not
sandals, bedrooms shoes, etc.),
long pants, and a shirt with long
sleeves or a jacket is best.
Nature Trail Safety
• Listen for the whistle!
• Return to meeting spot
immediately.
• No horse play (climbing trees,
running, climbing under or on
fallen trees, etc.)
Nature Trail Safety
• Report any injuries, stings, rashes,
etc. to the teacher.
• When you get home remove clothing
and check thoroughly for ticks.
• Ticks should be pulled gently from
the skin with tweezers.
Plant Safety in
South Carolina
Poison Ivy
• Identifying Characteristics:
– 3 leaflets
– Climbing vine
– Bright red leaves in the fall
• Danger/Caution:
– Skin irritation for some people
Poison Oak
• Identifying Characteristics:
– 3 leaflets
– Smaller shrub like
– Leaves have oak-like shaped lobes.
• Danger/Caution:
– Skin irritation for some people
Poison Sumac
• Identifying Characteristics:
– Compound leaf
– Orange-red stem w/ green leaves
• Danger/Caution:
– Skin irritation for some people
Cow Itch / Trumpet Creeper
• Identifying Characteristics:
– Climbing vine
– Reddish trumpet shaped flowers
• Danger/Caution:
– Skin irritation for some people
Virginia Creeper
• Identifying Characteristics:
– Climbing vine
– 5 leaflets
– Reddish in the fall
• Danger/Caution:
– Flowers & Leaves poisonous to eat
Yellow Jasmine
• Identifying Characteristics:
– Climbing vine
– Yellow flowers
– SC State Flower
• Danger/Caution:
– Flowers & Leaves poisonous to eat
Rhododendron / Mtn.
Laurel
• Identifying Characteristics:
– Looks like the azaleas that people
have in their yards. Same family.
– Shrub with small oblong dark green
leaves with flowers (white to red)
• Danger/Caution:
– Leaves & flowers are an internal
poison if eaten.
Rattlebox / Crotalaria
• Identifying Characteristics:
– Weedy plant w/ yellow flower
– Seed pods dry and rattle
• Danger/Caution:
– Seeds are poisonous if eaten and can kill
a horse.
Rattlebox
Rattlebox
Pokeweed (Pokeberry)
• Identifying Characteristics:
– Tall broadleaf weed. Head high
– Common in waste areas (vacant
lots, etc.)
• Danger/Caution:
– Roots are poisonous
– Leaves are poisonous if not
cooked correctly.
Nightshade (Deadly
Nightshade)
• Identifying Characteristics:
– Flowers look like the flowers on tomato
plants.
– Small fruit that look like little yellow
tomatoes
– Soft, hairy spines on stems
• Danger/Caution:
– Internal poison if any part is eaten.
Nightshade
Black Cherry
• Identifying Characteristics:
– Spindly shrub/small tree
– Little green berries which ripen to a
dark color
• Danger/Caution:
– The fruit is okay but the seeds are
poisonous and contain cyanide.
Black Cherry
Azalea
• Identifying Characteristics:
– Common flowering shrub around
homes.
– White, pink, salmon, and/or red
flowers
• Danger/Caution:
– Leaves and flowers are poisonous if
eaten.
Daffodils
• Identifying Characteristics:
– Yellow to white trumpet (cup & saucer)
shaped flowers that grow from a bulb.
• Danger/Caution:
– Bulbs are poisonous if eaten
Wisteria
• Identifying Characteristics:
–Climbing vine with grape-like clusters
of purple flowers.
–Velvety, long seed pods
• Danger/Caution:
–Seeds are poisonous if eaten
Chinaberry Tree
• Identifying Characteristics:
– May be found as a shrub or a tree
– Green berries that turn a waxy (bone)
color and become squishy.
• Danger/Caution:
– Berries can be poisonous if eaten.
Boxwood
• Identifying Characteristics:
–Evergreen shrub with small leaves
and thin woody stems. Often found
around homes.
• Danger/Caution:
–Leaves are poisonous if eaten.
Poinsettia
• Identifying Characteristics:
– Green and red or white leaves that
mimic flowers.
– Sold at Christmastime but there is a
small variety that also grows wild in
SC.
• Danger/Caution:
– Leaves are poisonous if eaten.
Mistletoe
• Identifying Characteristics:
–Evergreen plant that grows as a
parasite in trees.
–White, waxy berries
• Danger/Caution:
–Leaves and berries are poisonous
Holly
• Identifying Characteristics:
–Waxy, shiny green leaf top with
spines
–Varies from a shrub to tree size
–Red berries
• Danger/Caution:
–Berries are poisonous if eaten.
Jimsonweed (Thorn Apple)
• Identifying Characteristics:
–Small, weedy plant found
commonly in waste areas
–White flowers
–Spiny fruit pod
• Danger/Caution:
–All parts are extremely
poisonous if eaten.
Thorn Apple