General embryology Langman’s Medical Embryology, 13th Ed Jun Zhou (周俊) School of Medicine, Zhejiang University 20151225 a science which study the processes and regulations of development of human fetus.(from 1 cell (zygote) to (5-7) x1012 cells) In general the length of pregnancy is considered to be 280 days or 40 weeks after the onset of the last menstruation, or more accurately, 266 days or 38 weeks after fertilization. Onset of last M.C. Fertilization 14 days 266 days Labor 280 days Onset of M.C. Ovulation Total 38 weeks: Preembryonic Embryonic Fetal period: before 2 weeks period: 3-8 weeks period: after 9 weeks Preembryonic period: before 2 weeks Embryonic period: 3-8 weeks Fetal period: after 9 weeks Fertilization Definition: the process by which the male and female gametes(sperm and ovum) unite to give rise to zygote place: in the ampullary region of the oviduct Prerequisite for fertilization: 1) maturation of oocyte 2) maturation of spermatozoa 3) capacitation and acrosome reaction of spermatozoa: • capacitation factor: secreted by epi. cells of oviduct, capacity of releasing acrosome enzymes 4) quality and quantity of spermatozoa: • 2-6 ml, 100,000,000/ml, • <1.5 ml; or <10,000,000; abnormal sperm >30%; or capacity for mobile< 70% 5) meeting of sperm and ovum within 24 hrs Oocyte maturation Primordial primary Zona pellucida Antrum – secondary Cumulus oophorus Secondary oocyte – ovulation, polar body Fate of oocyte Spermatogenesis Germ cells dormant until puberty At puberty become mitotically active sertoli cells, nutrition, protection and mechanical support Unlike oogenesis, 4 gametes per clone Spermiogenesis Structural transformation Condensation of the nucleus Reduction of associated cytoplasm Formation of the neck, middle piece and the tail Formation of the enzymerich Acrosome overlying the head Abnormalities of gametogenesis Oocytes Multiple per follicle multiple gestations Multinucleated - die Spermatocytes Prerequisite for fertilization: 1) maturation of oocyte 2) maturation of spermatozoa 3) capacitation and acrosome reaction of spermatozoa: • capacitation factor: secreted by epi. cells of oviduct, capacity of releasing acrosome enzymes 4) quality and quantity of spermatozoa: • 2-6 ml, 100,000,000/ml, • <1.5 ml; or <10,000,000; abnormal sperm >30%; or capacity for mobile< 70% 5) meeting of sperm and ovum within 24 hrs Processes of fertilization: •Phase I –penetrate the corona radiata, acrosomal release •Phase II –ZP with receptors – zona pellucida spongy – the release of local enzymes •Phase III –Success formation of zygote • Entry – response 1) Cortical or zona reactions Compaction - impermeable to more sperm 2) Oocyte finishes 2nd meiotic division 3) Female /Male pronucleus 4) Metabolic activation of the egg • Results of fertilization – Diploid – Genetic sex – Cleavage Cleavage •Mitosis and cytoplasmic division •Blastula - blastomeres Morula inner cell mass embryoblast outer cell rim trophoblasts Blastocyst (No ZP) Blastocoele Inner cell mass Trophablasts Uterus at Time of Implantation •Puberty – menstruation (28 days) •Hormonal regulation –GnRH –FSH –LH •Granulosa/theca cells –Estrogens –Progesterones •The endometrium of the uterus is in the secretory phase Implantation -Passage through the uterine tube •Day 5 –Day 11 Uterus •Orientation of implantation - ant or post, fundus •Trophoblasts (overlying the embryoblast pole) attach to the endometrium, begin to invade. Processes zona pellucide disappear → polar trophoblast touch the endometrium→secrete enzymes →dissolve the endometrium → embedded into endometrium → coagulation plug seal the space •Implantation site –Normal: ant or post wall of the uterus –Abnormal –Tubal (most common) Ectopic pregnancy –Ovarian –Abdominal –Internal os of the cervix •Endometrium →decidua: -Decidua basalis -Decidua capsularis -Decidua parietalis •Prerequisite: -Endometrium is in secretory phase -Morula/blastocyst reach the cavity of uterus on time -Zona pellucide disappears in time Bilaminar Germ Disc (Second week of development) •Implantation - sinks deeper •The outer cell layer –Trophoblasts •cytotrophoblasts •Syncytiotrophoblasts (Syncytium) •Inner cell mass (embryoblast) –Fluid collection – amnion –Epiblasts & hypoblasts Week two – Day 9-12 •Implantation –Covered the endometrium- fibrin plug –Complete – slight bulge •Trophoblasts –Vacuoles within syncytiotrophoblastsfuse and form lacunar networks –Uteroplacental circ. •Extra-Embryonic Mesoderm –btwn troph & cavity –Somatic/splanchnic Week 2 – Day 13-14 •Trophoblasts –Primary villi •Yolk sac –Primary to seconday –Connecting stalk The Bilaminar Embryo •Disc btwn –Amnion –Yolk sac •Bilaminar disc –Epiblasts –Hypoblasts End of the Week of Twos •2 major cell masses –Trophoblasts –Inner cell mass •Trophoblasts –Cytotrophoblasts –Syncytiotrophoblasts •Embryonic Cavities –Amniotic cavity –Yolk sac •Germ Disc –Epiblasts –Hypoblasts OBJECTIVES 1. The definition of Embryology 2. The major phases of fertilization and the site where fertilization typically occurs 3. Endometrial changes that enable implantation and the hormones that modulate this change 4. Normal sites of implanation and the most abnormal sites 5. Morphologic changes in the zygote that occur enroute to the uterus 6. The development roles of the inner cell mass and the outer cell mass 7. Bilaminar germ discs