Supporting Table S1: The clinical profile, hematological parameters and the seropositivity
of the patients with Non Severe Dengue and Severe Dengue.
Characteristics
Gender
Clinical
Non- Severe Dengue
(n=35)
Severe Dengue
(n=13)
N (%)
N (%)
Male
15 (43)
7 (54)
Female
20 (57.1)
6 (46.1)
Positivity for tourniquet test
12 (34.3)
10 (76.9) #
Petechiae
7 (20)
12 (92.3)#
Third space collection
(pleural/peritoneal)
4 (11.4)
3 (23.1)#
Abdominal pain
8 (22.9)
4 (30.8)
Vomiting
12 (34.3)
9 (69.2)#
Headache
15 (42.9)
4 (30.8)
Platelet count (cells/mm3)
50037.04 ± 25464.12
29076.92 ±21554.05#
NS1 Ag
16 (45.7)
8 (61.5)
Anti-dengue IgG antibody
28 (80.0)
12 (92.3)
RT PCR (Viremia)
22 (62.9)
7(46.2)
Anti-dengue IgM antibody
15 (42.9)
10 (76.9)
Characteristics
Hematological
parameters
Positivity for
dengue
# indicates p < 0.05 in comparison with non-severe dengue patients.
Chi-square test was used to analyze the statistical differences between the clinical characteristics
of the study subjects with non-severe dengue and severe dengue. To assess the difference
between Platelet counts of study groups, Mann-Whitney U test was performed.
Supporting Table S2: Levels of Ceruloplasmin at the Day of admission (DOA) and at the
day of defervescence (DOD) in all dengue Fever patients with or without different
clinical characteristics.
Sl
No
1.
2.
Clinical
characteristics
Cases in
numbers
Ceruloplasmin
Headache & Retro
P = 30
30.06 ± 13.08
orbital pain
A = 18
21.23 ± 11.64
Vomiting &
P = 39
26.32 ± 12.14
Abdominal pain
A =9
28.62 ± 17.69
P = 19
21.88 ± 13.66
A = 29
29.94 ± 12.01
P = 10
26.71 ± 13.70
A = 38
26.76 ± 13.20
P = 11
14.40 ± 6.59
N = 37
30.42 ± 12.40
P = 23
21.96 ± 12.94
N = 25
31.15 ± 11.97
P = 25
26.45 ± 14.29
N = 23
27.07 ± 12.12
3.
Rash / Bleeding
4.
Fluid accumulation
5.
Tourniquet test
6
Thrombocytopenia
7
NS1 Antigen test
levels at DOA
P value
0.025
n.s
Ceruloplasmin
levels at DOD
34.04 ± 12.89
n.s
0.000
0.005
n.s
33.40 ± 7.15
33.09 ± 10.09
n.s
37.69 ± 11.93
36.94 ± 9.74
0.016
P value
n.s
29.85 ± 9.75
35.43 ± 11.69
n.s
33.07 ± 9.88
39.04 ± 8.43
29.28 ± 9.60
35.30 ± 9.68
0.021
n.s
30.94 ± 11.07
n.s
34.82 ± 8.37
n.s
32.61 ± 12.02
Results were expressed as mean ± standard deviation. P value was calculated using
Independent students’T’ test and Mann Whitney U test for normal and non normal distribution
respectively. P< 0.05 considered as statisitcally significant.
P: Presence of symptoms / Positive for test; A: absence of symptoms; N: Negative for test;
DOA: Day of Admission; DOD: Day of Defervescence. n.s: non significance
Supporting Table S3: Levels of Alpha 1 antitrypsin at the Day of Admission (DOA) and at
the Day of defervescence (DOD) in all dengue fever patients with or without different
clinical characteristics.
Sl
No
1.
2.
3.
Cases in
numbers
Headache & Retro
P = 30
123.70 ± 50.52
orbital pain
A = 18
187.44 ± 97.79
Vomiting &
P = 39
150.64 ± 86.19
Abdominal pain
A =9
186.82 ± 56.55
P = 19
152.99 ± 60.11
A = 29
158.67 ± 107.28
P = 10
133.56 ± 43.60
A = 38
163.82 ± 93.09
P = 11
154.12 ± 65.40
N = 37
156.78 ± 97.69
P = 23
167.06 ± 96.60
N = 25
135.46 ± 49.44
P = 25
159.44 ± 62.69
N = 23
151.71 ± 101.92
Rash / Bleeding
4.
Fluid accumulation
5.
Tourniquet test
6
Thrombocytopenia
7.
Alpha 1
antitrypsin
Clinical
characteristics
NS1 Antigen test
P value
levels at DOA
Alpha 1
antitrypsin
P value
levels at DOD
n.s
207.13 ± 141.86
301.07 ± 203.84
n.s
232.73 ± 170.87
389.45 ± 195.08
n.s
252.89 ± 59.14
0.047
n.s
0.036
255.55 ± 263.90
n.s
203.89 ± 70.62
272.93 ± 203.23
n.s
265.74 ± 54.33
244.40 ± 240.50
n.s
281.50 ± 183.73
n.s
0.017
n.s
206.15 ± 168.00
n.s
270.72 ± 139.09
n.s
237.48 ± 215.82
Results were expressed as mean ± standard deviation. P value was calculated using
Independent students’T’ test and Mann Whitney U test for normal and non normal distribution
respectively. p< 0.05 considered as statisitcally significant.
P: Presence of symptoms / Positive for test; A: absence of symptoms; N: Negative for test;
DOA: Day of Admission; DOD: Day of Defervescence; n.s: non significance
Supporting Table S4: Statistical differences of acute phase proteins using ROC analysis for
differentiating severe and non-severe dengue disease at admission
Variables
AUC±S.E
95% CI
(Lower limit –
Upper limit)
Cut-Off
value
Sensitivity (%)
Specificity
(%)
P
value
Youde
n
Index
+LR
─
LR
4.62
0.28
Ferritin
0.783 ± 0.103
0.558 – 0.928
>866.5
76.92
83.33
0.0005
0.602
Ceruloplasmin
0.758 ± 0.109
0.531 – 0.913
≤ 13.3
46.15
100
0.0390
0.461
Alpha1
antitrypsin
0.558 ±
0.1323
0.334 – 0.767
>171.67
40
83.33
0.659
0.233
0.54
2.40
0.72
AUC: Area Under Curve; S. E: Standard Error; CI: Confidence Interval; LR: Likelihood Ratio.
Supporting Table S5: Statistical differences of acute phase proteins using ROC analysis for
differentiating severe and non-severe dengue disease at defervescence
AUC ± S.E
Asymptotic
95% CI
(Lower limit –
Upper limit)
Cut-Off
value
Sensiti
-vity
(%)
Specif
-icity
(%)
P
value
Youden
Index
+LR
─
LR
Ferritin
0.958 ± 0. 038
0.777 – 0.999
>722.9
90
91.67
<0.001
0.8167
10.80
0.11
Alpha1
antitrypsin
0.800 ± 0.112
0.577 – 0.938
>179.49
90
75
0.0076
0.6500
3.60
0.13
Ceruloplasmin
0.779 ± 0.101
0.553 – 0.926
>25.2
90
58.33
0.0059
0.4833
2.16
0.17
Variables
AUC: Area Under Curve; S. E: Standard Error; CI: Confidence Interval; LR: Likelihood Ratio.