AP Biology
Belton High School
2013-2014
AP BIOLOGY
Unit Five Agenda—DNA
Day
Topic
Objectives
11/4-11/5
Central Dogma
History of DNA
1-2
11/6
Structure of DNA & RNA
Prokaryotic vs. Eukaryotic Genomes
3-6
11/7
Replication
Meselson and Stahl Case Study
7-8
11/10
Restriction Enzymes
Electrophoresis
9-11
11/11
Lab: Electrophoresis
11/12
Lab: Electrophoresis
11/13
Wrap Review
11/14
Unit Test
AP Biology
Belton High School
2013-2014
Unit Objectives: DNA
At the end of this unit you should be able to:
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11.
Describe what is meant by the “central dogma.”
Describe the work of each of the following sets of people in the discovery of
DNA: Watson & Crick, Wilkins & Franklin, Hershey & Chase, Avery,
MacLeod & McCarty.
Compare the structure and size of eukaryotic and prokaryotic genomes
including: number and shape of “chromosomes,” cellular location, and
presence of plasmids.
List the components of DNA and RNA nucleotides and identify important
structural differences between DNA and RNA (include type of sugar,
number of strands and identity of nitrogenous bases).
Distinguish between the structures of purines and pyrimidines and identify
which of the nitrogenous bases found in DNA and RNA belong to each
group.
Describe the antiparallel structure of a DNA molecule including: the
difference between the 3’ and 5’ ends, the pairing of bases, the chemical
bond connecting nucleotides of each strand and the chemical bond holding
the two strands together.
Explain what it means to say that DNA replication is a semiconservative
process.
Describe the process of DNA replication (including the differential formation
of the leading and lagging strands) and explain the roles of helicase,
topoisomerase, DNA polymerase and ligase in the process.
Describe the natural role of restriction enzymes and explain HOW they act
to cut DNA.
Describe the process of gel electrophoresis and give examples of real world
applications of this technology.
Explain how restriction enzymes and gel electrophoresis are used to isolate
and order DNA fragments to allow for restriction fragment analysis (to create
a DNA fingerprint).