BCH 3000
PRINSIP BIOKIMIA
FOR PJJ
(Semester 2 -2012/13)
1
Kod/Nama Kursus :
BCM 3000 (4+0)
(Biokimia Asas)
Nama Pensyarah
Prof. Dr. Mohd Arif
Syed (MAS) -Penyelaras
:
Prof. Dato’ Dr. Abu Bakar
Salleh (ABS)
Jabatan
:
Biokimia
Jadual Kuliah
( Masa dan Tempat) :
2
Sinopsis
(This course encompasses the main
biomoleculer components in biochemistry.
Metabolism involving the anabolism and
catabolism of major biomolecules are also
explained)
3
Learning Outcome
1. Distinguish the structure and function of
biomolecules found in biological systems (C4)
2. State the various key metabolic processes (P2)
3. Describe the biochemical reactions (A3)
4. Solve problems related to the metabolism of
biomolecules by using information from various
sources (CTPS, LL)
4
Brief Lecture Contents
1. Introduction-Biochemistry? Contributions? Important
life components
2. Carbohydrates – Classification – mono, di
polysaccharides – Structure –configuration &
stereochemistry; reactions – glucose and other sugars
3. Amino acid & protein – biological roles, structure,
classification, reactions, analysis. Peptides – primary,
secondary, tertiary and quaternary structures
4. Lipid –functions & distribution, characteristics of fatty
acids-saturated & unsaturated f/acids. Structures &
characteristics of triacylglycerols, phospholipids,
sphingolipids, terpenes & steroids
5
Brief Lecture Contents
5. Nucleic acids – components – purines, pyrimidines.
Structure, reactions & importance of nucleosides,
nucleotides & polynucleotides. DNA, RNA – structure,
functions & types
6. Enzymology – Classification, naming, active sites. Enzyme
kinetics. Factors affecting enzyme activity- enzyme &
substrate concentration, pH, temperature . Substrate
specificity – single & multiple substrate. Enzyme inhibitors
– competitive, con-competitive, uncompetitive. Control of
enzyme reactions – product inhibition, Isoenzymes,
multienzyme system and allosteric enzymes
6
Brief Lecture Contents
7. Carbohydrate metabolism – Metabolic energy cycle –
Bioenergetics: ATP other high energy compounds.
Storage & energy transfer. Glycolysis & fermentation.
Electron transport system. Compartmentation &
mitochondria. Phosphorylation & production of ATP.
Anaplerotic reactions. Glyoxylate cycle.
Gluconeogenesis. Pentose phosphate pathway.
Integration and control.
8. Photosynthesis – Fixation of CO2 during
photosynthesis. Chlorophyll, components of
photosynthesis. Photosystem I & II.
Photophosphorylation. Calvin cycle. Hatch-Slack
cycle.
7
Brief Lecture Contents
9.
Lipid metabolism – Lipid oxidation- Enzymes involved,
energy production. Oxidation of saturated & branched
fatty acids. Formation of ketone bodies. Lipid
biosynthesis –mitochondrial system and extramitochondrial. Cycle & enzymes involved. Synthesis of
saturated & unsaturated fatty acids. Cholesterol
synthesis & control.
10. Protein & amino acid metabolism – Degradation of
amino acids- transamination, deamination,
decarboxylation. Cycle involved- intermediates for the
TCA cycle. Ammonia and urea metabolism. Biosynthesis
of amino acids- role in the metabolism of porphyrin and
nucleic acids. Nitrogen fixation.
8
Brief Lecture Contents
11. Nucleic acid metabolism – synthesis of
mononucleotides – purines, pyrimidines – cycle and
enzymes involved; control. Biosynthesis of ribo &
deoxyribonucleotides. Characteristics of genetic
materials – chromosomes. Genetic code, base
sequence. DNA replication. DNA repair. Protein
synthesis – ribosome, co-factor involved & phase of
synthesis. Inhibition and control of synthesis.
12. Membrane Biochemistry – Modification & structure.
Model for membrane structure. Transport mechanism
across membrane – passive & active transport.
9
Brief Lecture Contents
13. Hormones- Introduction to plant & animal hormones.
Reactions & control of endocrine hormones. Hormone
reactions at the molecular level.
14. Integration & control o f metabolism. Relationship
between carbohydrate, lipid and protein metabolism.
10
Course Evaluation
1. Mid Term
= 35%
2. Final Exam
= 45%
3. SCL
= 20%
Total
= 100%
11
Course Evaluation
Mid Term
= 35% - 5, 6, 7 April 2013
Topics covered in test
1. Introduction-Biochemistry
2. Carbohydrates
3. Amino acid & protein
4. Lipid
5. Nucleic
6. Enzymology
12
Course Evaluation
Mid Term
= 35% - 5, 6, 7 April 2013
Types of Questions
1. Duration – 2 hours
2. Multiple choice – 60 questions (1 mark each)
3. Short Answers - Choose 8 out of 10 questions ; 5
marks each
13
Course Evaluation
Final Examination
= 45%
Topics covered in exam
7. Carbohydrate metabolism
8. Photosynthesis
9.
Lipid
10. Protein & amino acid metabolism
11. Nucleic acid metabolism
12. Membrane
13. Hormones
13. Integration & control of metabolism
14
Course Evaluation
SCL (Student Centered Learning = 20%
In this exercise, each student is required to produce a
model of an oligopeptide using materials from the
environment. The model should be able to demonstrate
clearly the structural configuration of the oligopeptide .
The student will be asked to present the model and
explain the structural configuration
15
Course Evaluation
SCL (Student Centered Learning = 20%
Model Requirements
1. The student must design and produce a model of an
oligopeptide
2. All amino acids must be different from one another and
of different group
3. Materials used must be from the environment. No model
kit will be allowed. This is also not computer modeling
4. The model should clearly show the structure of the amino
acid
5. Student will be asked to explain their respective models
16
Course Evaluation
SCL (Student Centered Learning = 20%
PRESENTATION
1. Week 14
2. Place – Biotek 1
3. Date – please inform when you are available
4. Evalauation by a panel
17