A Pharmacodynamic Evaluation of Switching from Ticagrelor to Prasugrel in Subjects with Stable Coronary Artery Disease: Results of the SWAP-2 Study Dominick J. Angiolillo, MD, PhD; Nicholas Curzen, BM (Hons), PhD; Paul Gurbel, MD; Paul Vaitkus, MD, MBA; Fred Lipkin, PharmD; Wei Li, PhD; Joseph A. Jakubowski, PhD; Marjorie Zettler, PhD, MPH; Mark B. Effron, MD; and Dietmar Trenk, PhD Journal of the American College of Cardiology (2014), doi: 10.1016/j.jacc.2013.11.032 Disclosures Dominick J. Angiolillo: Received payment as an individual for: a) Consulting fee or honorarium from Bristol-Myers Squibb, Sanofi-Aventis, Eli Lilly, Daiichi Sankyo, Inc., The Medicines Company, AstraZeneca, Merck, Evolva, Abbott Vascular, and PLx Pharma; b) Participation in review activities from Johnson & Johnson, St. Jude, and Sunovion; c) he has received institutional payments for grants from Bristol-Myers Squibb, Sanofi-Aventis, GlaxoSmith Kline, Otsuka, Eli Lilly, Daiichi Sankyo, Inc., The Medicines Company, AstraZeneca, Evolva; Gilead; and has other financial relationships with Esther and King Biomedical Research Grant Funding/Support: This study was sponsored by Daiichi Sankyo, Inc., and Eli Lilly and Company Introduction More potent platelet P2Y12 receptor inhibitors (prasugrel and ticagrelor) have been developed with improved efficacy, albeit increased bleeding, compared with clopidogrel In certain clinical situations, switching from ticagrelor to prasugrel may be considered • e.g. patients who experience dyspnea or use of a oncedaily dosing regimen due to adherence issues However, the pharmacodynamic (PD) effects of switching from ticagrelor to prasugrel are unknown Introduction The dissociation rate of ticagrelor and its active metabolite from the P2Y12 receptor would determine if, when switching to prasugrel, there is: • no change in level of platelet inhibition • additive platelet inhibition • blunted/delayed platelet inhibition The aim of the study was to compare the PD effects of switching from ticagrelor to prasugrel in subjects with stable CAD Methods Prospective, randomized, multicenter, international (12 sites), open-label study in subjects with stable CAD on ASA therapy • Blinded platelet function assessment (VerifyNow® P2Y12 assay and VASP) Primary endpoint • PRU by VerifyNow® for prasugrel 10 mg QD MD and ticagrelor 90 mg BID MD after 7 days of randomized treatment Secondary endpoints • PRI by VASP assay 2, 4, 24, and 48 hours and 7 days • PRU by VerifyNow® P2Y12 assay 2, 4, 24, and 48 hours • Percentage of subjects with HPR, defined as ≥230 PRU and ≥208 PRU by the VerifyNow® P2Y12 assay, and >50% PRI by the VASP assay ASA= aspirin; BID=twice-daily; CAD= Coronary Artery Disease; HPR=high on-treatment platelet reactivity, MD=maintenance dose; PRI=platelet reactivity index, PRU=P2Y12 reaction units, VASP-P=vasodilator stimulated phosphoprotein phosphorylation assay; QD= once-daily Study Hypothesis Hypothesis • Platelet reactivity (PRU) after 7 days of randomized treatment would be non-inferior in subjects who switched from ticagrelor to prasugrel compared with subjects treated continuously with ticagrelor using 45 PRU as the non-inferiority margin for the upper 95% CI limit of the difference Sample size • Based on the objective of establishing non-inferiority of prasugrel 10 mg QD MD (based on combined prasugrel-treated subjects) compared with ticagrelor 90 mg BID MD • Assuming a zero difference in mean PRU between treatment groups, a common standard deviation of 60 PRU, and a drop-out rate not exceeding 15%, a sample size of 105 allows for the 95% CI of the treatment difference to stay within 45 PRU with a probability of 0.90 BID=twice-daily; MD=maintenance dose; PRU=P2Y12 reaction units; QD=once-daily SWAP-2: Study design Patients with stable CAD on low-dose ASA not indicated for P2Y12 receptor antagonist Baseline platelet function studies followed by ticagrelor 180 mg LD/90 mg MD every 12 h post LD Run-in phase 3 – 5 days Platelet function studies 12 h post last ticagrelor MD and prior to randomized study drug (pre-randomization baseline) Prasugrel 60 mg LD + 10 mg MD QD Prasugrel 10 mg MD QD Ticagrelor 90 mg MD BID Platelet function studies at 2, 4, 24 and 48 hours and 7 days post first dose of randomized study drug ASA=aspirin, CAD=Coronary Artery Disease; BID=twice-daily, h=hours, LD=loading dose, MD=maintenance dose, QD=once-daily SWAP-2: Subjects Inclusion criteria Exclusion criteria Male and female subjects aged 18-75 y, ≥60 kg, stable CAD, low dose daily ASA (75-150 mg) for ≥7 days prior to screening Need for P2Y12 receptor antagonist therapy within 12 months of an ACS or PCI Stable CAD defined as Use of antiplatelet agents except ASA (warfarin, NSAIDs, or COX-2 inhibitors) • History of positive stress test • Previous coronary revascularization • Angiographic demonstration of CAD Strong inducers/inhibitors of CYP3A4 • At least moderate plaque by CT angiography High doses of simvastatin or lovastatin (>40 mg/d) • Electron beam CT coronary artery calcification score ≥100 Agatston units Active peptic ulcer History of TIA/stroke ACS=Acute Coronary Syndrome, ASA=aspirin, CAD=Coronary Artery Disease; COX-2=cyclooxygenase-2, CYP=cytochrome P450, CT=computed tomography, NSAIDs=nonsteroidal anti-inflammatory drugs, TIA=Transient ischemic attack SWAP-2: Subject Disposition Subjects screened (N=167) Subjects enrolled in run-in period (N=120) Subjects randomized (N=110)* Prasugrel 60 mg LD + 10 mg MD QD (Safety population) (n=34) Prasugrel 10 mg MD QD (Safety population) (n=41)* Ticagrelor 90 mg MD BID (Safety population) (n=35) Primary analysis population (n=31) Primary analysis population (n=34) Primary analysis population (n=33) *4 subjects randomized to Prasugrel 60 mg LD/10 mg MD group inadvertently only received MD and were included in the Prasugrel 10 MD group for analyses. BID=twice-daily, LD=loading dose, MD=maintenance dose, QD=once-daily. SWAP-2: Baseline Demographics* Treatment Group Prasugrel 60 mg LD + 10 mg MD (n=31) Prasugrel 10 mg MD (n=34) Total Prasugrel (n=65) Ticagrelor (n=33) P value† 57.5 (10.1) 58.9 (8.5) 58.2 (9.3) 61.8 (7.0) 0.057 22 (71.0) 24 (70.6) 46 (70.8) 25 (75.8) 0.641 Weight, mean (SD), kg 96.9 (18.0) 95.1 (19.0) 96.0 (18.4) 88.3 (18.0) 0.054 BMI, mean (SD), kg/m2 32.9 (6.0) 32.5 (6.1) 32.7 (6.0) 29.2 (4.7) 0.004 Diabetes 14 (45.2) 11 (32.4) 26 (38.5) 5 (15.2) 0.021 HTN 24 (77.4) 25 (73.5) 49 (75.4) 22 (66.7) 0.473 Hyperlipidemia 30 (96.8) 29 (85.3) 59 (90.8) 29 (87.9) 0.729 2 (6.5) 1 (2.9) 3 (4.6) 2 (6.1) 1.00 Characteristic Age, mean (SD), y Male, n (%) Medical History, n (%) Peripheral arterial disease *primary population, †total prasugrel group vs ticagrelor SWAP-2: Primary Endpoint 150 LS mean difference = 46.0 95% CI = 24.9, 67.2 p<0.001 PRU (mean ± SD) 125 100 75 50 25 0 Prasugrel total Ticagrelor After 7 days of randomized treatment • The upper 95% CI limit exceeded 45 PRU and did not reach the primary noninferiority endpoint • Sensitivity analysis correcting for imbalances in baseline characteristics did not alter the results SWAP-2: PRU Over Time 350 300 Prasugrel 60 mg LD/ 10 mg MD Prasugrel 10 mg MD Prasugrel Total Ticagrelor PRU (mean ± SD) 250 200 230 208 150 100 50 0 Pre-Run-In Baseline Pre2 hrs Post 4 hrs Post 24 hrs Post 48 hrs Post 7 Days Post Rand. First First First First First Baseline Rand. Rand. Rand. Rand. Rand. Dose Dose Dose Dose Dose • PRU increased at 24 and 48 hours in the prasugrel MD only group relative to pre-randomization values • Smaller increase in the prasugrel LD group compared with the prasugrel MD only group • PRU was higher in the prasugrel total group compared with the ticagrelor group at 7 days postrandomization SWAP-2: PRI Over Time Prasugrel 60 mg LD/ 10 mg MD Prasugrel 10 mg MD Prasugrel Total Ticagrelor 100 90 80 PRI (mean SD) 70 60 50 50 40 30 20 10 0 Pre-Run-In Baseline PreRand. Baseline 2 hrs Post First Rand. Dose 4 hrs Post 24 hrs Post First First Rand. Rand. Dose Dose 48 hrs Post 7 Days Post First First Rand. Rand. Dose Dose • Results for PRI over time paralleled those of PRU • PRI was higher in the prasugrel total group compared with the ticagrelor group at 7 days post-randomization SWAP-2: HPR Status PRU 230 Prasugrel 60 mg LD/ 10 mg MD Prasugrel 10 mg MD Ticagrelor 100 Percent of Subjects 80 60 *p<0.01 vs. ticagrelor 40 * * 20 0 Pre-Run-in Pre-Rand. 2-Hr 4-Hr 24-Hr 48-Hr 7-Day • Rates of HPR were higher at 24 and 48 hours in both prasugrel groups. No differences in PRU were observed at 7 days • Similar findings were observed for PRU ≥208 SWAP-2: HPR Status PRI > 50% Prasugrel 60 mg LD/ 10 mg MD Prasugrel 10 mg MD Ticagrelor 100 Percent of Subjects 80 * 60 *p<0.01 vs. ticagrelor † p<0.05 vs. ticagrelor † 40 † 20 0 • Pre-Run-in Pre-Rand. 2-Hr 4-Hr 24-Hr 48-Hr 7-Day Rates of HPR were higher at 24 and 48 hours and at 7 days in both prasugrel groups SWAP-2: Adverse Events Both prasugrel and ticagrelor were well tolerated Serious adverse events: only in 1 patient (ticagrelor group) and were considered unrelated to study drug Incidence of dyspnea: • Prasugrel: 0% • Ticagrelor: 3.3% (n=4) during run-in phase; 2.9% (n=1) randomized Minor bleeding (mostly mild ecchymosis): • Prasugrel: 10.7% (n= 8) • Ticagrelor: 20.0% (n=7) No deaths or ischemic events observed during the course of the study SWAP-2 Conclusions SWAP-2 did not achieve the primary objective of demonstrating non-inferiority of PD response after switching from ticagrelor to prasugrel after 7 days of treatment Results suggest a PD interaction when switching from ticagrelor to prasugrel that is partially mitigated with administration of a LD of prasugrel This is associated with higher rates of HPR in the first 24-48 hours after switching, which diminishes by 7 days of treatment The optimal timing between the discontinuation of ticagrelor and the administration of a LD of prasugrel remains to be determined Thank you to all the SWAP-2 investigators and study coordinators Investigators Dr. Richard Anderson Paul Gurbel, MD University Hospital of Wales Sinai Center for Thrombosis Research Health Park, UK Sinai Hospital of Baltimore, US Dominick J. Angiolillo, MD, PhD Dr. Thomas Johnson University of Florida College of Medicine, US Bristol Heart Institute, UK Dr. James Cotton Douglas K. Logan, MD New Cross Hospital, UK Medpace Clinical Pharmacology Unit, US James Feldman, MD Samuel Oberstein, MD West Houston Area Clinical Trial Clinical Pharmacology of Miami, Inc., US Consultants, LLC, US Randeep Suneja, MD Professor Nicholas P. Curzen Cardiology Center of Houston, US Southampton General Hospital, UK James Walder, MD Professor Anthony H. Gershlick Black Hills Cardiovascular Research, US Department of Cardiovascular Science University of Leicester, UK Alexander White, MD Progressive Medical Research, US