UNIT 4 TOPIC 1 PHOTOSYNTHESIS 3: THE LIGHTINDEPENDENT REACTIONS Complete the notes sheets below. Where you see a blank (…………………..), decide which word/phrase is most appropriate. The light-independent reactions use the reducing power of ………………… and the chemical energy stored in ………………… (produced during the light………………… reactions). A series of reactions (the ………………… Cycle) takes place in the ………………… of a chloroplast. Carbon dioxide from the ………………… is ………………… to carbohydrates in a series of ………………… controlled reactions. 1. CARBON FIXATION: Molecules of ………………… from the air combine with (…..C) ribulose bisphosphate (…………………). The ………………… is now said to be ………………… . The highly abundant ………………… ribulose bisphosphate carboxylase/oxygenase (…………………) is 1|Page responsible for catalysing this step. (…..C) Glycerate-3-phosphate (…………………) forms from a highly unstable …..C intermediate. 2. REDUCTION: The molecules of (…..C) GP are phosphorylated using ATP, which destabilises the molecule and provides the ………………… ………………… the required GP to to (3C) glyceraldehyde-3-phosphate (…………………) using the reducing agent …………………. . The ………………… is ………………… back to NADP. 3. REGENERATION OF RuBP: Most of the (…..C) GALP passes through a series of steps to regenerate (…..C) ………………… using some of the ………………… produced during the light-………………… reactions. 4. Some ………………… is converted into (…..C) glucose. It takes several turns of the ………………… ………………… to produce 1 molecule of glucose. 2|Page The entire Calvin Cycle: PHOTOSYNTHESIS: A SUMMARY 3|Page Photosynthesis Summary Questions Explain the steps in the light dependent reaction below Photo-activation of Photosystem II Photolysis of water Electron transport Cyclic phosphorylation Non-cyclic phosphorylation Photoactivation of Photosystem I Reduction of NADP+ 4|Page Draw a diagram summarizing the light dependent reaction of photosynthesis . Explain phosphorylation in terms of chemiosmosis Explain the roles of the following in the light independent reaction RuBP Reduction of glycerate-3-phosphate to GALP NADPH + H+ ATP 5|Page Regeneration of RuBP Synthesis of carbohydrate Describe two ways that photosynthesis can be measured directly Describe one way that photosynthesis can be measured indirectly Outline the effects of the following on the rate of photosynthesis and explain the concept of limiting factors with reference to each Temperature 6|Page Light intensity Carbon dioxide concentration 7|Page