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What is your question?
(Be specific describe what you are going to try and find out)
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Why is answering your question important?
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What is the IV?
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What is the DV?
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Relevant Background knowledge
What do you already know about your IV? (from your own experiences, NOT what you read about in science class)
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What do you already know about your DV? (from your own experiences NOT what you have read about in science)
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– write down important information you learned from each bacteria article we read in science
Source 1  Bacteria Article 1 (Pink) Title: Click here to enter text.
Author and source (if given): Click here to enter text.
Important things that I learned that relate to my question
: (In your own words!!)
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Source 2  Bacteria Article 2 (Yellow) Title: Click here to enter text.
Author and source (if given): Click here to enter text.
Important things that I learned (In your own words!!)
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Source 3  Bacteria Article 3 (Green) Title: Click here to enter text.
Author and source (if given): Click here to enter text.
Important things that I learned (In your own words!!)
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State a formulized hypothesis.
(Don’t forget to include a specific prediction!!)

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because Click here to enter text.

List of materials you will use:
(be specific and accurate)
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1 Sterile Petri Dish pre-filled with Nutrient Agar
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2-4 Sterile cotton swabs
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Tape
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Permanent marker
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Scotch tape
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Figure 1. Diagram of Petri dish set-up and sample key
Key: What’s in each section
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1 2
3 4
2
3
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Initials Core
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View of labels on the * Highlight your control group (if you have one) bottom of Petri dish
Numbered Experimental Procedure: Read the procedure below & Highlight all Constants or Controls
1.
Prepared Petri dishes should be refrigerated until used and always stored upside down (i.e. agar in upper dish, cover on bottom). This keeps condensation which forms in the lid from dropping onto and disrupting the bacteria growing surface.
2.
When ready to use, let dishes come to room temperature before taking samples (about one hour).
3.
Obtain one sterile Petri dish, pre-prepared with Nutrient Agar.
4.
Using a marker, draw lines on the bottom of your Petri dish, splitting it into 4 equal parts, and label as shown in Figure 1 above.
5.
Collect a bacteria sample by gently wiping sample # 1 with a sterile q-tip, then immediately inoculate section 1 of your Petri dish (as described below)
6.
Without tearing the agar surface, inoculate section #1 of the dish by gently wiping the bacteria laden q-tip across the agar surface in section #1 only.
7.
Quickly replace cover on dish after each inoculation to avoid contamination from airborne bacteria.
8.
Repeat steps 4 – 6 to gather and plate each of your different bacteria samples, using a new sterile Q-
tip for each of your bacteria samples.
9.
When finished plating all your samples, tape closed in 3 spots, and store upside down.
10.
Let grow in undisturbed location at ~ 70°F (room temperature) for 3-4 days. Bacteria can grow at any temperature from about ambient room temperature (hopefully around 70°F) all the way up to about
100°F. Do not place in sunlight or on a heated surface.
11.
Check Petri dish every day for 3-4 days and record the following data:
A.
Quantitative Data: Count the number of colonies
B.
Qualitative observations: like color, shape, size, smell, texture, etc.
C.
Draw a color picture of the Petri Dish
12.
Before disposing of dishes in the trash the bacteria should be destroyed. Pour a small amount of household bleach over the colonies while holding dish over sink. Caution - do not allow bleach to touch your skin, eyes or clothes. It will burn!

Quantitative Data : Presented in a data table *Don’t forget to give your data table an informative title!
Table 1: Click here to enter text.
Day 1
Day 2
Day 3
Section 1:
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(# of colonies)
Section 2:
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(# of colonies)
Section 3:
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(# of colonies)
Section 4:
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(# of colonies)
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Qualitative Data: daily written observations & drawings
Day 1
Day 2
Day 3
Qualitative Observations
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Drawing of Petri Dish