Habitat Isolation 2 species live in the same area, occupy different habitats rarely encounter each other garter snakes Temporal Isolation 2 species breed at different times of the day or seasons Eastern and Western Spotted Skunks Behavioral Isolation Signals to attract mates are often unique to a species fireflies Mechanical Isolation Closely related species attempt to mate, but are anatomically incompatable flowering plants with pollination barriers Gametic Isolation Sperm and egg must recognize each other fertilization of fish eggs Prezygotic Barriers Postzygotic Barriers Reduced Hybrid Viability Reduced Hybrid FertilityHybrid Breakdown Hybrid Breakdown abort development of hybrid at some embryonic stage Meiosis doesn't produce fertile gametes in vigorous hybrids First generation hybrids are fertile, but they can't produce fertile offspring in the next generation subspecies of salamanders horse + donkey = sterile mule rice, cotton (f1 fertile, but not f2)