Catalase Rubric

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Section Heading
Introduction
o Opening paragraph that introduces the activity and outlines the body paragraphs
o Transition sentences between paragraphs are used
o Define the term catalyst
o Characteristics of a catalyst
o Re-usable
o Reduce activation energy
o Specific substrate
o Inorganic vs. Organic
o Characteristics of Enzymes (Organic Catalyst)
o Composed of Proteins
o Association between active site and tertiary structure
o Factors affecting Enzyme Rate of Reaction
o
Temperature (low vs. high; high denatures (define) and low slows reaction)
o
pH
o
Concentration of substrate (with constant enzyme concentration)
o
Concentration of enzyme (with constant substrate concentration)
o
Competitive inhibitor
o
Non-competitive inhibitor
o How Enzymes Work
o
Induced Fit Model
o
Coenzymes
o Hydrogen Peroxide
o
What is it
o
When is it produced
o
Reaction to detoxify it
o Problem
o Hypothesis
o In-text citations (you must cite where you got the information about characteristics of catalysts
and enzymes in addition to the factors affecting catalytic ability. Your notes and textbook are
not enough. Do not use .com websites for information. You may use primary and other
secondary sources)
Materials
o Include materials for Problem 3 only.
o "The materials used in this experiment were ...."
Methods
o Report only the methods for Problem 3
o Paragraph form
o Past tense
o Passive voice
Example: A piece of liver was placed into three small test tubes. Two ml of HCl was added to
test tube 1, 2 ml of distilled water was added to test tube 2 and 2 ml of NaOH was added to
test tube 3. The liver pieces were left to soak for 10 minutes. After ten minutes the liquid was
poured out of the three test tubes and 5 ml of H2O2 was added.
Results
o Recreate the tables using Excel or Word
o Title each table (descriptive title; number each table)
o Short written summary of all results. (refer to table when summarizing)
Discussion
o Include analysis of all the laboratory data
o Statement about if your hypothesis was supported or disprove
o Refer back to information in the introduction when supplying reasons for data
o Include citations to support your explanations (again no .com sources)
Point
Value
2
1
1
1
1
1
1
1
2
2
1
1
1
1
1
2
1
1
1
1
1
1
1
2
1
2
2
5
5
2
1
1
1
1
Points
Earned
o
o
o
o
Main findings (Liver, potato and MnO2catalysts of H2O2; temperature and pH affect organic
catalysts; enzymes are larger than substrates; catalysts are reusable)
Explain results of Table 1
o
(Based on the results of Table 1 Liver, potato and MnO2catalysts of H2O2 and
NaCl is not a catalyst. Liver, ….. led to the creation of oxygen bubbles).
o
This is evidence of the reusable property of catalysts (give the evidence and if
inconsistent – not the same for each trial – try to explain why catalytic ability
differed per trial).
o
Compare catalytic ability of liver, potato and MnO2
Explain results of Table 2
o
Why did the results differ between temperature (denaturing or slowing
chemical reactions)
o
Normal body temperature of a cow (relate to enzyme rate and if your
expectations of what should have happened differs)
Explain results of Table 3
o
Why did the results differ between pH (denaturing or slowing chemical
reactions)
o
If your results differ from what is expected try to explain why your results
differ
Explain results of Table 4
Sources of potential error and effects of errors on your results
Future question (next step)
Conclusion paragraph (quick summary of experiment and results)
o
o
o
o
Format
o Double spaced
o 12 font
o Sections labeled and bold faced
o Grammar (Paragraph structure, sentence structure)
o Spelling
o Stapled
o Biological vocabulary used
o APA citations (Reference Section, last page)
5
5
5
5
5
5
1
2
1
1
2
5
2
1
5
5
105
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