EDINBORO UNIVERSITY OF PA
GENETICS BIOL 300-002 (FALL 2014)
DEPARTMENT OF BIOLOGY AND HEALTH SERVICES
PROFESSOR: Dr. William J. Mackay
OFFICE: Cooper Hall Room 168
TELEPHONE: 732-2926
OFFICE HOURS: M W 11:00 – 11:30 AM (Porreco)
M 4:30 – 6:00 PM; Tu Th 4:45 – 6:00 PM (CH168)
Email: wmackay@edinboro.edu
TEXT: Genetics (Analysis & Principles) (5th edition), Brooker, McGraw-Hill (2015) ISBN-13:
978-0-07-352534-1
CLASS NOTES: http://www.wjmmackay.com/notes2.html
TENTATIVE LECTURE/LAB SCHEDULE
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
RELEVANT READINGS
Tu 26 AUG Overview of Genetics
Chapter 1
Mendelian Inheritance
Chapter 2
Th 28 AUG Mendelian Inheritance
Chapter 2
LAB: Introduction To Explorer Program
Tu 02 SEP Mendelian Inheritance
Chapter 2
Extensions of Mendelian Inheritance
Chapter 4
Th 04 SEP LAB: 1 Genetic Probability
Tu 09 SEP Due: Lab Report 1 (Genetic Probability)
Extensions of Mendelian Inheritance
Chapter 4
Reproduction & Chromosome Transmission
Chapter 3
Th 11 SEP LAB: 2 Exploring Genetics
Tu 16 SEP Due: Lab Report 2 (Exploring Genetics)
Variation in Chromosome Structure & Number
Chapter 8
Th 18 SEP LAB: 3 The Dihybrid Cross
Tu 23 SEP Due: Lab Report 3 (The Dihybrid Cross)
Genetic Linkage & Mapping in Eukaryotes
Chapter 6
Molecular Structure of DNA & RNA
Chapter 9
Th 25 SEP LAB: 4 Meiosis and Crossing Over/ 5 Mapping by Recombination Frequency
Tu 30 SEP Due: Lab Report 4, 5 (Meiosis/Recombination)
DNA Replication
Chapter 11
Chromosome Organization & Molecular Structure
Chapter 10
Th 02 OCT EXAM 1
Tu 07 OCT Gene Transcription & RNA Modification
Chapter 12
Translation of mRNA
Chapter 13
Transcriptional Regulation
Chapter 15
Th 09 OCT LAB: 6 Sex Linkage
Tu 14 OCT READING DAY (NO CLASS)
16 Th 16 OCT
17 Tu 21 OCT
18 Th 23 OCT
19 Tu 28 OCT
20 Th 30 OCT
21 Tu 04 NOV
22
23
24
25
Th
Tu
Th
Tu
06 NOV
11 NOV
13 NOV
18 NOV
26 Th 20 NOV
27 Tu 25 NOV
28 Tu 02 DEC
29 Th 04 DEC
30 Th 11 DEC
LAB: 7 Other Modes of Inheritance
Due: Lab Report 6 (Sex Linkage)
Due: Lab Report 7 (Other Modes of Inheritance)
Gene Mutation & DNA Repair
Chapter 18
LAB: BONUS LAB 1: Control of Gene Expression
Due: Bonus Lab Report 1 (Control of Gene Expression)
Genetic Transfer & Mapping in Bacteria & Bacteriophages Chapter 7
Gene Regulation in Bacteria
Chapter 14
Genetics of Viruses
Chapter 17
LAB: BONUS LAB 2: Mitosis & the Cell Cycle
Due: Bonus Lab Report 2 (Mitosis & the Cell Cycle)
Recombination & Transposition at the Molecular Level
Chapter 19
Epigenetics
Chapter 16
Review for Exam 2
EXAM 2
Recombinant DNA Technology
Chapter 20
LAB: 8 Population Genetics & the Effect of the Environment
Due: Lab Report 8 (Population Genetics & the Effect of the Environment)
Biotechnology
Chapter 21
Genomics I: Analysis of DNA
Chapter 22
Medical Genetics & Cancer
Chapter 24
BONUS LAB 3: DNA Fingerprinting
Due: Bonus Lab Report 3 (DNA Fingerprinting)
Medical Genetics & Cancer
Chapter 24
Population Genetics
Chapter 26
Non-Mendelian Inheritance
Chapter 5
Review for Exam
MAKE-UP EXAM
FINAL EXAM (6-8)
1. Determination of grade:
A: 90 – 105
B+: 85 – 89
B: 80 – 84
C+: 75 – 79
OPTION 1
Quizzes* (see below)
Exam 1
Exam 2
Final Exam
Lab Reports** (see below)
Attendance*** (see below)
20
20
20
20
20
0-5
TOTAL
105
OPTION 2
Quizzes* (see below)
Exam 1
Exam 2
Final Exam
Attendance*** (see below)
TOTAL
20
25
25
30
0-5
105
C: 70 – 74
D+: 65 - 69
D: 60 – 64
F: < 60
2. *Lecture quizzes: There will be periodic ten-minute quizzes that will cover lecture material
since the previous quiz. Quizzes will count 20% toward your final course grade. No make-up
quizzes will be given (for any reason).
3. Examinations: Examinations 1, 2, and the Final Exam WILL be given on the dates
specified. Any question format may be expected on the exams. Examinations will be primarily
based on information presented in the lecture/lab sessions. Each exam, however, will also
contain information that will be drawn from textbook readings and handouts that may not have
been directly covered in lecture or lab. Thus, it is to your benefit to read the assigned chapters in
your textbook.
4. ***Absences: Students may attend lectures and labs at their own discretion. In accordance
with University policy, attendance will be taken at the beginning of each lecture/lab meeting
(where applicable). Students who maintain a perfect lecture/lab attendance will receive five
bonus points to your final grade. Students that have 1 absence (for any reason) will earn 3
points and students that have 2 absences (for any reason) will earn 1 point to your final grade. It
is my experience that students with 3 or more absences rarely obtain a grade higher than C. In
short, it is to your benefit to attend all lecture and lab sessions.
5. **Lab reports: Eight (8) lab assignments will be administered during the semester. In
addition, there are three (3) bonus lab assignments. Lab report average will be determined as
follows: each assignment will receive one of the following grades.
Grade assigned:
Requires:
100%
No errors. Every answer must be complete and, where possible,
must use experimental data to correctly illustrate the answers.
95%
No errors. No more than 1-2 incomplete answers and/or failures
to illustrate answers using experimental data
90%
No more than one error. No more than 1-2 incomplete answers
and/or failures to illustrate answers using experimental data
87%
No more than two errors.
84%
No more than three errors.
80%
No more than four errors.
70%
No more than five errors.
60%
No more than six errors.
50%
No more than seven errors.
40%
No more than eight errors.
Sample question & suggested answer: An experiment was conducted using
Edinboronites to determine if, like humans, the sex ratio among offspring is 50:50 female
to male. 300 Edinboronite couples were mated. Each couple produced one offspring
which was sexed. 152 of the offspring were male and 148 were female. What is your
conclusion?
Suggested answer: "It would appear that the ratio is very close to a 1:1 (50:50) ratio. If it had
been perfect, there should have been 150 males and 150 females but instead we obtained
152:148. Just like tossing a coin 300 times, we do not expect to get exactly 150 heads and 150
tails every time because each occurrence is randomly determined. Therefore, occasionally we
will get more heads than tails or vice versa and sometimes we will get an equal number.
However, it is possible, that we have not tested enough specimens to be sure. Perhaps this ratio
does indicate a bias towards males (we know this occurs in humans)."
Comment: The above answer does not simply answer the question by saying no (or for
that matter yes). Whether you answered that it supports a 50:50 or that it does not, you would
have a correct answer. However, the added data and explanation is what separates the "A"
answer from a non-A answer.
(lab report % X .20 = final lab report points) (e.g. 90% X .20 = 18.0 final lab report points)
Bonus Labs will count up to 1 point (100%) toward your final lab grade! Thus, you can
add up to 3 points (23/20) to your final lab score.
Written Lab Reports are due on the day at 6:00PM (unless otherwise specified).
There will be NO CREDIT for a late report!
Electronic lab reports will not be accepted!
6. CD – ROM Addresses for Genetics Labs
Introduction To Explorer Program: Logal/ Genetics/“Genetics Main Menu”/First Look.
Genetic Probability: Explorations/Genetic Probability.
Lab Report: http://www.wjmmackay.com/uploads/4/2/7/2/4272503/genetic_probability.doc
Exploring Genetics: Explorations/Introduction.
Explorations/Mendelism.
Explorations/Testing Mendel.
Lab Report: http://www.wjmmackay.com/uploads/4/2/7/2/4272503/exploring_genetics.doc
The Dihybrid Cross: Explorations/Dihybrid Crosses.
Lab Report: http://www.wjmmackay.com/uploads/4/2/7/2/4272503/the_dihybrid_cross.doc
Meiosis and Crossing Over: Core Inquires/Meiosis.
Core Inquires/Linkage and Variation.
Lab Report:
http://www.wjmmackay.com/uploads/4/2/7/2/4272503/meiosis_and_crossing_over.doc
Sex Linkage: Core Inquires/Sex linkage.
Lab Report: http://www.wjmmackay.com/uploads/4/2/7/2/4272503/sex_linkage.doc
Other Modes of Inheritance: Core Inquires/Variations to Dominance.
Core Inquires/Three's a Crowd.
Core Inquires/Fatal Flaw.
Lab Report:
http://www.wjmmackay.com/uploads/4/2/7/2/4272503/other_modes_of_inheritance.doc
Control of Gene Expression: Open the file "Gene Regulation" of the program "Explorations in Cell
Biology and Genetics" (George Johnson ISBN 0-697-29214-2 WCB McGraw-Hill). Click on
Wcb_pc_(D:)/Startbio/Menu/Gene Regulation.
Lab Report:
http://www.wjmmackay.com/uploads/4/2/7/2/4272503/control_of_gene_expression.doc
Population Genetics: Independent Investigations/Environmental Effects.
Lab Report: http://www.wjmmackay.com/uploads/4/2/7/2/4272503/population_genetics.doc
DNA Fingerprinting: Open the file "DNA Fingerprinting" of the program "Explorations in Cell Biology
and Genetics" (George Johnson ISBN 0-697-29214-2 WCB McGraw-Hill). Click on
Wcb_pc_(D:)/Startbio/Menu/DNA Fingerprinting.
Lab Report: http://www.wjmmackay.com/uploads/4/2/7/2/4272503/dna_fingerprinting.doc
Bonus Lab: Mitosis & the Cell Cycle: Open the file "Mitosis" of the program "Explorations in Cell
Biology and Genetics" (George Johnson ISBN 0-697-29214-2 WCB McGraw-Hill). Click on
Wcb_pc_(D:)/Startbio/Menu/Mitosis.
Lab Report: http://www.wjmmackay.com/uploads/4/2/7/2/4272503/cell_cycle.doc
RELATED LINKS:
1.
Isolation of Plasmid DNA:
http://www.wjmmackay.com/uploads/4/2/7/2/4272503/isolation_of_plasmid_dna.doc
http://www.wjmmackay.com/uploads/4/2/7/2/4272503/purelink_quick_plasmid_dna_miniprep.doc
2.
Gel Electrophoresis:
http://www.wjmmackay.com/uploads/4/2/7/2/4272503/agarose_gel_electrophoresis.doc
3.
Restriction Mapping:
http://www.wjmmackay.com/uploads/4/2/7/2/4272503/restriction_digests.doc
4.
Microbiology Techniques:
http://www.wjmmackay.com/uploads/4/2/7/2/4272503/techniques_in_microbiology.doc
5.
Transformations:
http://www.wjmmackay.com/uploads/4/2/7/2/4272503/transformations.doc
6.
Example of a Serial Dilution:
http://www.wjmmackay.com/uploads/4/2/7/2/4272503/example_of_a_serial_dilution.doc
7. Make-up work: Students are required to take all exams and to complete all assignments on
time. Exceptions will be made in rare and unusual circumstances and only by PRIOR
arrangement. Each case will be considered individually. Make-up exams, when justified, will be
given at the earliest mutually convenient time or during make-up week.