Sodium Pyruvate
Sodium pyruvate is added to many media formulations as a
carbohydrate source that cells can easily access for energy
production. It is also involved in amino acid metabolism and
aerobic respiration.
Molecular Structure
Pyruvate is an anion of pyruvic acid and the end product of
glycolysis. The glycolytic pathway, which occurs in the
cytosol of aerobic organisms, converts glucose to pyruvate,
which then undergoes oxidative decarboxylation in mitochondria to form acetyl CoA, the metabolite that initiates
the Kreb’s Cycle.¹ Some microorganisms convert pyruvate
to ethanol through the energy-producing process of
alcoholic fermentation when under anaerobic conditions.²
Prior to it’s conversion to acetyl CoA, pyruvate may also be
used to produce alanine by transamination. In the synthesis of
alanine, pyruvate is the amino-group acceptor.1
Our Sodium Pyruvate solution is offered as a 100 mM sterile
solution in cell culture grade distilled, deionized water. A
dilution of 1:100 to yield a 1mM (110 mg/L) working
concentration is suitable for most cells.
References
1. Moran, L.A., and Scrimgeour, K.G. Biochemistry. 2nd Ed.
New Jersey: Prentice Hall, 1994. pp. 15.1-21.15.
2. Keeton, W.T. Biological Science. 5th Ed. New York:
Norton and Company, 1993. p. 168-181.
Product Specifications
pH
5.5 ± 0.5
Endotoxin
450 ± 50 mOsm/kg H2O
Mycoplasma
≤ 0.25 EU/mL @ 1:50
Sterility by USP
Tested negative
Cell Culture Testing
Pass
Molecular Weight
146.15
Formula
C5H10N2O3
Appearance
Clear, colorless solution
Working
Concentration
1 mM (110 mg/L)
Storage and Stability Shelf life of 18 months when stored refrigerated 2ºC to 8°C, protected from light.
Shipping
Ambient (15ºC to 30°C)
Cat. No.
Description
25-000-CI
Sodium Pyruvate 100 mM solution
Size
Qty/Pk
100 mL
6
Corning cellgro products are manufactured by Mediatech, Inc., a Corning Subsidiary
The Corning Family of Brands
®
cellgro®
Mediatech, Inc.
A Corning Subsidiary
®
For a complete listing of trademarks, visit us at www.cellgro.com/about-us/trademarks.html
Corning Incorporated, One Riverfront Plaza, Corning, NY 14831-0001
®
9345 Discovery Blvd.
Manassas, VA 20109
t 800.235.5476
f 703.471.0363
www.cellgro.com
© 2012 Corning Incorporated Printed in U.S.A. 3/12 POD CLS-CG-TS-357
When cells are under conditions of oxidative stress, pyruvic
acid is reversibly reduced to lactic acid, resulting in the
formation of NAD+. Pyruvate also takes on antioxidant
duties in low oxygen environments.