Campbell Chapter 20 - DNA Technology
1. What are restriction enzymes and what natural purpose do they serve?
2. What is a vector? Name two common vectors that are used in DNA technology.
3. Briefly outline the steps involved in using bacteria and plasmids to clone a gene.
4. How is cDNA made and why is it sometimes necessary?
5. How can a probe be used to locate a gene of interest once it has been cloned?
6. Explain how gel electrophoresis can be used to separate and visualize DNA fragments.
7. Describe the Sanger method for sequencing DNA.
8. Describe the polymerase chain reaction.
9. Describe the techniques of Northern and Southern blotting.
10. How are these techniques useful?
11. How can restriction fragment length polymorphisms be used as genetic markers?
12. Briefly explain how DNA technology can used in each of the following areas:
a) diagnosis of disease
b) gene therapy
c) vaccines and pharmaceuticals
d) forensics
f) environmental applications
g) agricultural uses
13. Briefly discuss some ethical and safety concerns surrounding the use of DNA technology.
Adapted From: KVHS Biology - Kevin Gallant
Rothesay, N.B.