LABORATORY FOR EXPERIMENTAL
EXPERIMENTAL BOTANY
VECNA POT 111
SI-1000 LJUBLJANA, SLOVENIA
http://botanika.biologija.org/exp/
UNIVERSITY OF LJUBLJANA
BIOTECHNICAL FACULTY
DEPARTMENT OF BIOLOGY
contact email: ales.kladnik@bf.uniales.kladnik@bf.uni-lj.si
PHOSPHATE BUFFERED SALINE (PBS)
10x PBS (sodium-potassium combo), 1 L:
•
2 g KCl
•
2.4 g KH2PO4
•
80 g NaCl
•
11.45 g Na2HPO4
note: if Na2HPO4 (7H2O), use 21.6g, if Na2HPO4 (12H2O), use 28.8g.
Dissolve everything in approximately 800 ml of dH2O, then fill up to 1000 ml.
Autoclave and store at room temperature.
10x PBS, pH 7˙4 (sodium), 100 mL:
•
7,6 g NaCl (Mw 58.4425 g/mol, M = 1,3 mol/L)
•
0,99 g Na2HPO4 (Mw 141.959 g/mol, M = 0,07 mol/L)
•
0,41 g NaH2PO4 x H2O (Mw 137.992 g/mol, M = 0,03 mol/L)
Adjust pH with 1 M NaOH or 1 M HCl. Fill up to 100 ml. Autoclave and store at room
temperature.
Calculation of Mw: CHEMIX (http://home.c2i.net/astandne/help_htm/chemix.htm)
Last change: 2007-02-01
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