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LSSG-CT-2004-013032
ELIfe
European Lipidomics Initiative: Shaping the Life Sciences
Instrument: Specific Support Action
Thematic Priority: FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6
TOWARDS A EUROPEAN LIPIDOMICS PROGRAM
Publishable Final Activity Report
Final Report
2005-2007
Period covered: from 1/1/06 to 1/7/07
Start date of project: 1/1/05
Project coordinator name:
Project coordinator organisation name:
Date of preparation: 1/1/08
Duration: 2.5 years
Prof. Gerrit van Meer
Utrecht University
TOWARDS A EUROPEAN LIPIDOMICS PROGRAM
Publishable Final Activity Report
Contents
1. Project execution
page
3
Project Objectives
4
Contractors Involved (for the duration of the project)
5
Work Performed and End Results
6
2. Dissemination and use
8
References
9
Annex 1 - Survey of expertise and infrastructure within the
Lipidomics field
Annex 2 - Plan for Using and Disseminating Knowledge
(extended version)
Annex 3 - Plan for Using and Disseminating Knowledge
(publishable version)
Annex 4 - Final management report (pending)
Annex 5 - Final report on the distribution of the Community's
contribution (pending)
Annex 6 - Questionnaires
2
TOWARDS A EUROPEAN LIPIDOMICS PROGRAM
1. Project execution
The research areas that deal with the major chemical constituents that build up the cells in our
body are referred to as genomics for genes and nucleic acids, proteomics for proteins and
glycomics for carbohydrates. What has been missing in the “OMICS” realm is lipidOMICS.
Since both carbohydrates and lipids are cellular metabolites, glycomics and lipidomics are
subdivisions of metabolomics.
The time has come for generating broad insights into the role of lipids in physiology and
pathology. Given that thousands of different lipids are present within a single cell and that
many of these lipids are involved in modulating the processes of life in an area that is
upcoming, lipidomics describes and quantitatively analyses the full complement of lipids, in
for example body fluids, cells and tissues. Lipidomics integrates these data with knowledge of
their protein targets, i.e. the metabolic enzymes and transporters, and of the relevant genes
and the regulatory aspects of these physiological systems. Above all, an understanding of cell
membranes will not be possible without understanding their lipid constituents.
Most important is the fact that many of the widespread diseases that plague humankind
involve lipids. Prime examples are cardiovascular disease, obesity-related type-2 diabetes, and
stroke. Other major diseases such as cancer and Alzheimer's disease also have a lipid
involvement. In addition to these disorders of epidemic proportions, there are many other
diseases that are directly caused by inherited defects in lipid metabolic enzymes and
transporters, such as defects in cholesterol synthesis and lipid storage diseases. Lipids also
play major roles in autoimmune diseases and act as (co-)receptors for bacteria, viruses and
toxins. An increase in our knowledge of disease-related changes in lipid patterns and its
integration into proteomic and genomic data will provide new basic biomedical insights; thus,
far-reaching possibilities for diagnostic application (prognostic assessment, diagnosis and
monitoring) as well as for the development of prevention and new therapeutic approaches can
be expected.
Although lipid research was well developed in the initial era of biochemistry in the 1960s and
70s, it lagged behind the more recent developments of genomics and proteomics. One major
problem has been the lack of technology to analyze the thousands of different lipids in body
fluids and cellular systems. However, this problem is now quickly being overcome by
exquisitely sensitive high-throughput mass spectrometric methods that are revolutionizing the
field. Still, the application of such techniques to solving basic biomedical problems has
remained rare and the clinical use of the potential of lipidomics is sparse. Finally, at the start
of the present initiative there were no fora where basic biomedical scientists and clinicians
would meet the technology developers, bioinformaticians and industrial stakeholders who can
provide the infrastructure and the standardized kits needed for clinical testing.
3
Project Objectives
The primary aim of the European Lipidomics Initiative (ELIfe) was to mobilize and organize
key stakeholders, researchers and end-users in the area of metabolomics, especially lipidomics
research, and to further define this field of research in terms of participants, scientific contents
and strengths.
Objective 1: To network the field of metabolomics and to seek alliances with relevant
stakeholders
The consortium should network and position the field of Lipidomics. Other areas of
Metabolomics research like Glycomics and Signalomics (signaling lipids, lipid hormones)
would be involved in all activities. Researchers, stakeholders from the health care profession
and from industry with special emphasis on SMEs would be invited to join ELIfe activities at
the earliest possible stage.
Objective 2: To link the field of metabolomics to the genomics and proteomics initiatives
One special challenge was to adapt bio-informatics as devised for Genomics and Proteomics
to the field of Metabolomics. The choice of the technology to be used would define the type
of bio-informatics approaches necessary to deal with the large body of data expected from
Lipidomics and in a wider sense Metabolomics.
Objective 3: To define a strategy for metabolomics research, using lipidomics as example
Lipidomics was subdivided into three areas of development: i) technology, ii) cell biology,
and iii) health. Experts in these areas were to be joined in a series of workshops to define
where these areas would gain most from a European collaboration and what direction of
research would most likely bring breakthroughs. The topic of lipid rafts would be taken as a
theoretical test case to define the kinds of experimental data that can be generated by highthroughput approaches in this field, and to determine how these data sets could be most useful
for medical application.
Objective 4: To initiate an Expertise Platform on Lipidomics
As a first step to fully mobilize the field, an awareness of the strength and expertise was to be
created. Via a survey of expertise, to be published on the web and via a specific workshop on
Lipidomics and Technology, a virtual Expertise Platform would be initiated and linked to the
Euro Fed Lipids organization. It was to become a test centre for bench marking of new
Lipidomics technology.
Objective 5: To hold both science-related as well as policy meetings
A series of scientific and strategic meetings had to be organised. (a) Workshops with a focus
on technology and terminology, on basic science and on medical applications of
metabolomics and of lipidomics in particular. (b) Networking meetings and applications
meetings should join academic scientists across the life sciences, clinical laboratory scientists,
clinicians and industrial representatives. (c) The concluding conference should be open to
policy makers as well as the above mentioned representatives, and specific round tables were
to be organized for this purpose.
4
Contractors Involved (for the duration of the project)
Partic.
No.
Participant name
Participant
short name
Address
Country
1
Gerrit van Meer
UU-CBLE
Utrecht University
2
Gerd Schmitz
UniReg
University Hospital Regensburg
D
3
Kai Simons
MPI-CBG
MPI-CBG, Dresden
D
4
Jürgen Borlak
Fh-ITEM
ITEM, Hannover
D
5
Raymond Dwek
OGBI
Oxford University
UK
6
Pam Fredman
UGOT
Göteborg University
7
Felix Goñi
UPV-EHU
University of the Basque Country, Leioa
8
Elina Ikonen
UniHsk
University of Helsinki
NL
S
E
FIN
9
Michel Lagarde
INSERM
INSA Lyon
F
10
Konrad Sandhoff
KI
University of Bonn
D
11
Balázs Sarkadi
NMC
National Medical Center, Budapest
12
Fritz Spener
Sepp Kohlwein
UniGraz
University of Graz
13
Sandro Sonnino
UniMi
University of Milano
I
14
Gerd Utermann
IMBHG
Medical University Innsbruck
A
HU
A
Co-ordinator:
Prof.dr. Gerrit van Meer
Bijvoet Center
T:
+31-30-253.3427
Utrecht University
E:
g.vanmeer@uu.nl
Padualaan 8
3453 CH Utrecht
Project manager:
Dr. Bas R. Leeflang
Bijvoet Center
T:
+31-30-253.3498
Utrecht University
E:
b.r.leeflang@uu.nl
Padualaan 8
3453 CH Utrecht
Project website: www.lipidomics.net
Lipidomics Expretise Platform: www.lipidomics-expertise.de
5
Work Performed and End Results
Expertise Platform and the inventory of stakeholders (Objectives 1 and 4).
The Lipidomics Expertise Platform was launched on the internet in November 2005. The
database was used to prepare a survey of the lipidomics expertise and infrastructure in
Europe. A database containing 2,500 active e-mail addresses was used to mobilize
lipidologists for the concluding ELIfe meeting. Scientists from industry were specifically
invited for the first workshop (Dresden, 2005) focusing on lipidomics technology, which led
to the exchange of ideas on technological possibilities versus the needs of the scientists in the
field.
Contacts were established between ELIfe and related initiatives in the US (LipidMAPS) and
Japan (LipidBank). As a first collaborative step, a common lipid classification scheme has
been devised and published [1-3]. In this context, professors Spener and van Meer were
invited to become members of the International Lipid Classification and Nomenclature
Committee (ILCNC), which has taken on the task of updating and expanding the
classification system. The committee concluded its first meeting on July 7, 2006, with a list of
recommendations in the light of a continuously evolving inventory of biological lipid
structures.
Lipidomics versus genomics/proteomics and the need for bioinformatics (Objective 2).
Genomics, proteomics and metabolomics experts were invited to present their points of view
at the ELIfe kick-off meeting. The recognized need for specific bioinformatics approaches
was then addressed by inviting the lipid MAPS experts for the first technology workshop in
Dresden, and subsequently by active participation of ELIfe members (with LipidMAPS
representatives) in an extra workshop at the European Bioinformatics Institute in Hinxton that
concentrated on connectivity between different types of databases, the willingness of EBI to
provide the physical storage space for lipid databases, and the curation of existing databases.
This resulted in the involvement of EBI experts in the later ELIfe-based FP7 lipidomics
application LipidomicNet.
Devising a strategy for lipidomics research (Objective 3)
Experts in three areas of development of lipidomics: i) technology, ii) cell biology, and iii)
health, were collected in three workshops to discuss progress in their subfield and to define
what would be the best way to stimulate lipidomics research in Europe. Experts in technology
were brought into contact with basic scientists and clinicians at an industry workshop, that
was positioned as a satellite meeting to the concluding ELIfe/FEBS special meeting in
Noordwijkerhout (NL). The outcome of this meeting, which attracted over 60 scientists, was a
round table discussion on technologies available, on instrument development, and on
application of these in society, where possible in high throughput mode. A second workshop
in Bilbao (E), cosponsored by EMBO, brought together basic scientists in physics, chemistry
and biology who focused on the theme of lipid rafts, membrane substructures of exciting
functions, and the possibilities to further characterize these structures using novel
technologies. In a final workshop on 'Lipidomics and Health' preceding the 2006 ICBL
meeting in Pécs (HU), life scientists and clinical scientists discussed the applications of novel
and high-throughput lipidomics technology in medical applications.
In line with the workshops, the following papers were published, each of which included a
conclusion/perspective section: 'Cellular Lipidomics', 'Lipidomic strategies to study structural
and functional defects of ABC-transporters in cellular lipid trafficking' and 'The European
Lipidomics Initiative: Enabling Technologies' [4-6]. In addition, a white paper under the title
6
'Enabling Technologies for Studying the Genome, Proteome and Cytome of the Lipidome'
was published as an interactive paper on the ELIfe website [7]. A series of editorials by ELIfe
members served to critically evaluate future developments in lipidomics [8-13]. In addition,
ELIfe members formed an interest group with other lipid scientists to prepare a policy
document for the European Science Foundation under the title: 'Structural Medicine II: the
Importance of Lipidomics for Health and Disease' [14].
To pursue possibilities to apply for lipidomics funding under FP7 two meetings were
organized in 2006 and 2007 for ELIfe members plus other interested scientists in Frankfurt
(D). This eventually resulted in the successful FP7 application LipidomicNet. A EuroCORE
theme proposal EuroMEMBRANE by a team led by the ELIfe chair has been accepted by
ESF, with an expected call for research grant applications in early 2008 [15].
Science-related and policy meetings (Objective 5)
A series of scientific and strategic meetings has been organised. Besides the technology
workshop in Dresden (2005), and the three workshops in Bilbao, Pécs an Noordwijkerhout,
ELIfe contributed sessions to four industrial and life science meetings: (1) the 2005
conference of the European Life Sciences Organization (ELSO 2005) in Dresden (D), was
attended by 1,200 scientists, amongst whom 40% PhD students. The program consisted of 6
plenary sessions, 21 minisymposia, 3 poster sessions and 7 sub-group meetings. ELIfe
contributed Minisymposium 1: Lipidomics. (2) the 26th World Congress and Exhibition of
the International Society for Fat Research (26th ISF World Congress) in Prague (CZ) 2005,
hosted by the Czech Chemical Society and Euro Fed Lipid: 'Modern aspects of fats and oils A fascinating source of knowledge'. The session on 'Lipid Bioscience and Genomics' to this
industrial meeting was sponsored by ELIfe. (3) the 47th International Conference on the
Bioscience of Lipids (ICBL - ELIfe – ILPS joint meeting), 2006, in Pécs (HU): two sessions
on 'Lipidomics' and 'membrane microdomains' to an audience of basic lipid scientists. (4) the
4th Euro Fed Lipid Congress, 'Fats, Oils and Lipids for a Healthier Future - The Need for
Interdisciplinary Approaches', Madrid (E), 2006. One session was contributed on 'Lipid
Mediators and Lipidomics'. This was a meeting with an industrial character.
The concluding general ELIfe meeting 'New concepts in lipidology: from lipidomics to
disease' in Noordwijkerhout, NL, 2006, was cosponsored as a FEBS special meeting and was
attended by over 250 participants The meeting hosted a short joint symposium between the
Nordrhein-Westfälische Akademie der Wissenschaften and the Dutch Royal Academy of Arts
and Sciences. A special ELIfe issue of FEBS Letters under the title 'Lipidome and Disease'
was distributed at the meeting [16].
7
2. Dissemination and use
Over 2005 and 2006, the specific support action 'the European Lipidomics Initiative (ELIfe)'
has resulted in four successful workshops where stakeholders from basic science, industry and
medicine met to discuss cell biological, technological, industrial and clinical aspects of
lipidomics. In addition, ELIfe contributed lipidomics sessions to four networking meetings,
one on life sciences, one on the bioscience of lipids and two industrial lipid meetings. These
workshops and meetings culminated in the concluding general meeting in October 2006,
attended by a broad audience of 250 scientists. At the occasion of this meeting a special
journal issue was published on 'Lipidome and Disease' [16]. The results of the the specific
support action were reported in a series of policy papers in the scientific literature [4-6, 8-13],
and a policy briefing on lipidomics and health of the European Science Foundation [14]. In
addition, ELIfe contributed to a number of technical papers on lipid classification and data
handling [1-3] and to a 500 page document entitled 'Enabling technologies for studying the
genome, proteome and cytome of the lipidome.' [7]. This document will be presented on the
Lipidomics Expertise Wiki Portal (LEP-Wiki): http://www-cgi.uniregensburg.de/Klinik/Klinische_Chemie/lipidWiki/, to allow a direct interaction with the
scientists in the field. Interviews with ELIfe members appeared in various journals [17,18].
A survey was prepared based on registrations in the Lipidomics Expertise Platform
www.lipidomics-expertise.de with the purpose of identifying stakeholders and providing
insight in the available expertise, which can be used for contacting specific centers for
collaboration. A 260 page document entitled 'Lipid droplets and lamellar bodies as dynamic
organelles connecting influx, efflux, and storage of lipids: Translational research towards
human disease' was prepared as the basis for a grant proposal under FP7 by a number of
ELIfe members plus other scientists. This proposal 'LipidomicNet' was recently selected for
funding. A EuroCore theme proposal under the name EuroMembrane [15] has been accepted
by the European Science Foundation and a call for applications is expected for early 2008.
In summary, we believe that the European Lipidomics Initiative has created many
opportunities for crossover between basic science and medical and commercial applications,
and that it has inspired stakeholders to seek contact and establish strategic alliances. Because
national funding agencies and policy makers value the European dimension, the project will
impact on both the European and the national level in shaping policies and research activities,
both in applied and fundamental research. One such field is that of nutrition and health.
Technology development will allow more detailed analyses of lipid patterns in diseased and
healthy persons, which will drive discussions with the food industry concerning the potential
positive and negative effects of different types of (lipid) nutrition on human health with new
health policies drawn up as a result.
8
References
[1.a]
[1.b]
[2]
[3]
[4]
[5]
[6]
[7]
[8]
[9]
[10]
[11]
[12]
[13]
[14]
[15]
[16]
[17]
[18]
Fahy, E., S. Subramaniam, H.A. Brown, C.K. Glass, A.H. Merrill, Jr., R.C. Murphy,
C.R. Raetz, D.W. Russell, Y. Seyama, W. Shaw, T. Shimizu, F. Spener, G. van Meer,
M.S. Vannieuwenhze, S.H. White, J. Witztum, and E.A. Dennis (2005) A
comprehensive classification system for lipids. J. Lipid Res. 46, 839-861.
idem: (2005) Eur. J. Lipid Sci. Technol. 107, 337-364
Varfolomeyev, S., Efremenko, E., Beletskaya, I., Bertini, I., Blackburn, G.M.,
Bogdanov, A., Cunin, R., Eichler, J., Galaev, I., Gladyshev, V., O’Hagan, D., Haertle,
T., Jarv, J., Karyakin, A., Kurochkin, I., Mikolajczyk, M., Poroikov, V., Sakharov, I.,
Spener, F., Voyer, N., and Wild, J. (2005) Postgenomic chemistry (IUPAC Technical
Report). Pure Appl. Chem. 77, 1641–1654.
Spener F. (2005) Lipidomics and consequences: a new classification system for lipids.
Eur. J. Lipid Sci. Technol. 107, 277-278.
van Meer, G. (2005) Cellular Lipidomics. EMBO J. 24, 3159-3165.
Schmitz, G., Liebisch, G., Langmann, T. (2006) Lipidomic strategies to study
structural and functional defects of ABC-transporters in cellular lipid trafficking.
FEBS Lett. 580, 5597-5610.
van Meer, G., Leeflang, B.R., Liebisch, G,. Schmitz, G., Goni, F.M. (2007) The
European lipidomics initiative: enabling technologies. Methods Enzymol. 2007;432,
213-232.
White paper on 'Enabling technologies for studying the genome, proteome and cytome
of the lipidome.' to be published in Wikipedia format on the LEP website.
Spener F. (2005) European Commission funds lipidomics project. Eur. J. Lipid Sci.
Technol. 107, 1-2.
Griffiths, W. (2006) Why steroidomics in brain? Eur. J. Lipid Sci. Technol. 108, 707–
708.
Helms, B. (2006) Host-Pathogen interactions: Lipids grease the way. Eur. J. Lipid Sci.
Technol. 108, 895–897.
Spener, F., Kohlwein, S.D., and Schmitz, G. (2006) Lipid droplets and lamellar bodies
– from innocent bystanders to prime targets of lipid research for combating human
diseases. Eur. J. Lipid Sci. Technol. 108, 541-543.
Spener, F., Zechner, R., and Borlak, J. (2006) Is lipotoxicity an oxymoron? Eur. J.
Lipid Sci. Technol. 108, 625-627.
van Meer, G. (2006) How do sphingolipids and lipid rafts relate to pathology? Eur. J.
Lipid Sci. Technol. 108, 799–801.
van Meer, G. and Spener, F. (Co-Chairs), Leeflang, B.R. (Secretary), Beisiegel, U.,
Bougnoux, P., Goñi, F., Griffiths, W., Hartmann, T., Helms, B., Hoekstra, D., JuliàSapé, M., Larijani, B., Moschetta, A., Mouritsen, O.G., Norata, G.D., Payrastre, B.,
Record, M., Schmitz, G., Simons, K., Tselepis, A., Vaz, W., Vigh, L., Voelker, D.R.,
Wakelam, M.J.O., and Wanders, R.J.A. (2008) Structural Medicine II: the Importance
of Lipidomics for Health and Disease, European Science Foundation Policy Briefing,
in press.
van Meer, G. (main proposer), Malhotra, V., Marsh, M., Simons, K., van der Goot, G.,
Warren, G. (2008) Membrane Architecture and Dynamics (EuroMEMBRANE),
EuroCORE theme proposal. Call to be launched in spring 2008.
Helms, B. and van Meer, G., eds. (2006) Lipidome and Disease. FEBS Letters Special
Issue. FEBS Lett. 580, 5429-5610.
Winckler, L. (2005) Lipidomics. Laborjournal 12, 20-23.
Hillyer, C.D. (2006) Lipidomics: taking it one lipid at a time. Inform 17, 206-208.
9
10
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
LSSG-CT-2004-013032
ELIfe
European Lipidomics Initiative: Shaping the Life Sciences
Instrument: Specific Support Action
Thematic Priority: FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6
TOWARDS A EUROPEAN LIPIDOMICS PROGRAM
Survey of expertise and infrastructure within the Lipidomics field
Final Report
2005-2007
Date of preparation: 1/1/08
Start date of project: 1/1/05
Project coordinator name:
Project coordinator organisation name:
Duration: 2.5 years
Prof. Gerrit van Meer
Utrecht University
11
Work Package 2 (coordinated by Partner 2: Prof. Gerd Schmitz, Regensburg)
Deliverable 2.4:
Survey of expertise and infrastructure within the Lipidomics field
The following project objectives were implemented into the Lipidomics
Expertise Platform (LEP, http://www.lipidomics-expertise.de) :
-
Design an Expertise Platform on Lipidomics
-
Hold a survey of expertise and infrastructure with the Lipidomics field
Description of the Lipidomics Expertise Platform (LEP)
The Lipidomics Expertise Platform (LEP, http://www.lipidomics-expertise.de) was set up in
2005 (Fig. 1).
Fig. 1: Starting page and menu structure of the LEP
In order to gain full access institutions and users have to register with the LEP. Currently,
128 different scientific and 6 industrial institutions are registered in the LEP. These
institutions include a total number of 188 registered users. Most users belong to 18 different
European countries, although there are non-European registrations from 8 countries (Table
1). The contact details of institutions and user registered in LEP are attached in appendices 1
and 3, respectively.
12
Europe
Country
Austria
Belgium
Denmark
Finland
France
Germany
Greece
Hungary
Italy
Netherlands
Norway
Poland
Romania
Slovenia
Spain
Sweden
Switzerland
United Kingdom
scientific
10
4
1
6
21
33
1
7
14
21
1
1
1
1
10
5
4
22
Total
163
Users
industrial
1
1
2
1
1
Institutions
scientific
industrial
8
1
4
1
6
14
1
24
1
4
9
7
2
1
1
1
1
1
7
3
2
16
1
6
110
6
Non European Countries
User
Country
Argentina
Brazil
Canada
China
Israel
Japan
Taiwan
United States
scientific
2
1
2
1
1
3
3
12
industrial
Total
25
0
Institutions
scientific
industrial
1
1
2
1
1
2
2
8
18
0
Table 1: Users and Institutions registered in the LEP (as at 01.01.2008)
One aim of the Lipidomics Expertise Platform was to collect and provide information on labs
involved in lipidomics. Therefore the LEP contains various electronic surveys that collect
contact information and give an overview of the expertise present in the various labs. The
institutions registered were asked to complete electronic surveys on (Fig. 2):
-
Areas of lipidomics expertise
-
Lipid class skills
-
Technology skills
-
Organisms and cellular systems skills
13
Fig. 2: Wizard for electronic surveys
Fig. 3: Search menu for lipidomics expertise.
14
The results of these surveys are searchable in an advanced search menu (Fig. 3). A
statistical analysis of the survey results is shown in Table 2-5. Detailed survey results are
displayed in appendix 2 ordered by country.
Areas of Lipidomics Expertise
Lipid Metabolism
Analytical Tools in Lipidomics
Lipid Signalling
Membrane Microdomains
Mapping of the Lipidome
Genomics of the Lipidome
Lipids in Inflammation
Metabolomics of the Lipidome
Membrane Trafficking
Lipid Transport
Lipids and Nutrition
Signalomics of the Lipidome
Bioinformatics related to the Lipidome
Proteomics of the Lipidome
Pharmacology and toxicology of the Lipidome
Glycomics of the Lipidome
Lipids in Microbiology
Chemical Synthesis of Lipids (Probes/Tools/Agents)
Lipid Standard Bank
Nutrigenomics of the Lipidome
Lipids in Skin Care
37
36
23
22
19
19
19
18
18
17
17
11
10
9
8
7
7
7
7
6
2
Table 2: Survey on the areas of Lipidomics expertise; Institutions with medium and high
expertise level
Lipid Class - Survey
Fatty Acids and Conjugates [FA01]
Glycerophosphocholines [GP01]
Glycerophosphoethanolamines [GP02]
Eicosanoids [FA03]
Ceramides [SP02]
Diradylglycerols [GL02]
Glycerophosphoglycerols [GP04]
Docosanoids [FA04]
Glycerophosphoglycerophosphates [GP05]
Glycerophosphates [GP10]
Fatty aldehydes [FA06]
Fatty esters [FA07]
Triradylglycerols [GL03]
Phosphosphingolipids [SP03]
Neutral glycosphingolipids [SP05]
Sterols [ST01]
Glycerophosphoserines [GP03]
Glycerophosphoinositols [GP06]
Glycerophosphoinositol bisphosphates [GP08]
Acidic glycosphingolipids [SP06]
Bile acids and derivatives [ST04]
Glycerophosphoinositol monophosphates [GP07]
Steroids [ST02]
24
21
17
13
13
12
11
8
7
7
6
6
5
5
5
5
4
4
4
4
4
3
3
Table 3: Survey on lipid classes investigated; Institutions with medium and high expertise
level (only lipids classes with more than 2 hits)
15
Technology - Survey
ESI
Fluorescence Microscopy
Enzymatic Assays
LC
GC
Gel Electrophoresis
Light Microscopy
CI
Confocal Microscopy
LC-MS coupling
NCI
Thin layer chromatography
Normal Phase
GC-MS coupling
Real Time PCR
Reversed Phase
Ion Exchange
Liquid-Liquid Extraction
Cell Sorting
Immuno Assays
DNA Sequencing
Data Processing
EI
Quadrupole
Ion Trap
Database Setup
Cluster Analysis
Biostatistics
Immune Affinity
MALDI
Solid Phase Extraction
Microarrays
Chemical Synthesis
Size Exclusion
nano ESI
APCI
Electron Microscopy
Nuclear Magnetic Resonance
Multicolor Analysis
UV-Vis Spectroscopy
TOF
Electron Spin Resonance
Infrared Spectroscopy
Fluorescence Phosphorescence Spectroscopy
Capillary Electrophoresis
FTICR
QqTOF
Atomic Force
Capillary Electrophoresis
dHPLC
32
27
27
24
23
22
19
18
18
17
15
14
14
14
14
13
13
13
13
13
12
11
9
9
9
9
9
9
8
8
7
7
7
6
6
6
6
6
5
5
4
4
4
4
3
3
3
3
3
3
Table 4: Survey on the technologies used; Institutions with medium and high expertise level
(only technologies with more than 2 hits)
16
Organisms and cellular Systems - Survey
Human Cell Lines
Mice
Blood
Biopsy
Human Primary
Mouse
Rats
Yeast
Urine
Eubacteria
Rabbits
Arabidopsis
Agricultural Crop
Stool
27
22
22
18
15
15
14
14
10
5
4
4
3
3
Table 5: Survey on the areas of Lipidomics expertise; Institutions with medium and high
expertise level (only organisms with more than 2 hits)
Beside this contact and expertise database the LEP contains databases for lipid standards
(Fig. 4) and for methods (Fig. 5), which should improve methodology and standard material
exchange.
Fig. 4: Methodology Database.
17
Fig. 5: Lipid Standard Database.
Currently, as one goal of the FP project LipidomicNet the Wikipedia software is being
integrated into the LEP (LEP-Wiki, Fig. 6), which will provide information on lipids from basic
information to advanced aspects like the biology and pathophysiology that lipids are involved
in.
A major source for LEP-Wiki is a close link to other databases including LIPID MAPS
(http://www.lipidmaps.org) for lipid nomenclature and lipid species data, Lipid Bank - Japan
(http://www.lipidbank.jp) for lipid species data and KEGG PATHWAY Database for metabolic
pathways
(http://www.genome.jp/kegg/pathway.html).
Moreover,
information
on
lipid
metabolic pathways will be linked to genomics information including databases like Ensembl
(http://www.ensembl.org).
LEP-Wiki lives from the contribution of people interested in Lipidomics. All Lipidomics
Expertise Platform (LEP) members can edit the content and add new data.
18
Fig. 6: LEP-Wiki
19
Appendix 1: Contact details and description of institutions registered in LEP ordered by country (as per 01.01.2008)
Company
UNESCO Chair
Biophys. & Mol.
Neurobiol
Organisation
Universidad
Nacional del Sur
Inst. Biophysics
& X-ray
Structure
Research / Fct.
Lipidomics
Phone
054
2914861201
ZIP
8000
City
Bahia
Blanca
Street
C.C. 857
Country
Argentina
Austrian Academy ++43-316of Sciences
4120-323
A-8045
Graz
Schmiedlstrasse
6
Austria
BIOCRATES
Life Sciences
BIOCRATES Life
Sciences
6020
Innsbruck
Innrain 66
Austria
Institute for
Genomics and
Bioinformatics
Graz University of
Technology
++43-5125798234216
++43-316873-5345
8010
Graz
Petersgasse 14
Austria
Institute for
Molecular
Biotechnology
Institute of
Biochemistry,
Cell Biology
Group
Graz University of
Technology
++43-316873-4089
A-8010
Graz
Petersgasse 14/I
Austria
Graz University of
Technology
+43-316873-6462
A-8010
Graz
Petersgasse 12/2
Austria
Keywords
cell-surface receptors /
lipid-protein
interactions /
membranes / structure
/ dynamics
lipid polymorphism,
membrane biophysics,
membrane mimetic,
membranolytic
peptides
Metabolomics, Lipid
quantitation,
Bioinformatics
transcriptional
regulation,
microarrays,
adipogenesis,
lipotoxicity
yeast lipids, sterols
yeast, mitochondria,
lipid particles,
phospholipids, neutral
lipids
Description
biochemistry and biophysics of lipids cell-surface membrane structure
and dynamics lipid-protein interactions in nicotinic receptors lipids in
embryonic development lipids in neuronal differentiation and survival
lipids in retina lipids in plants lipids in reproductive system
Type
scientific
Our research aims at the elucidation of the molecular mode of action of
scientific
host defence peptides that affect cells by interacting in a non-specific
manner with their membranes and not via specific receptors. An
understanding of how these peptides distinguish between bacterial and
mammalian cell membranes will allow the design of novel peptide
antibiotics, which can selectively kill bacteria. Bacterial resistance to
such antimicrobial peptides is less likely to occur owing to the nature of
their target and fast killing kinetics. However, there has been evidence
that resistance to antibiotics may also arise due to changes in lipid
composition of their membranes. Therefore, the design of novel and
effective antimicrobial peptides will only be possible, if the entire lipid
spectrum of its membrane has been identified, which nowadays owing to
recent advances in analytical instrumentation can be addressed both at
the qualitative and quantitative level. Experimental approach membrane biophysics (structural, thermodynamic and spectroscopic
techniques)- lipid analysis of target membranes - peptide libraries
Targeted metabolomics Mass spectrometry Quantitation of phosphoindustrial
and glycolipids, eicosanoids etc. Bioinformatics and biostatistics
Biomarker discovery and validation
Transcriptional regulation of lipotoxic pathways. Gene expression
scientific
analysis of mouse models of lipid-associated disorders.
Lipid transport in yeast The roles of sterols in membrane transport in
yeast Sterol homeostasis in yeast
scientific
The main subjects studied in our group are synthesis of lipids and their
assembly into organelle membranes of the yeast Saccharomyces
cerevisiae. The majority of yeast lipids are synthesized in the
endoplasmic reticulum with some significant contributions of
mitochondria, the Golgi and the so-called lipid particles. Other
subcellular fractions, e.g. the plasma membrane, are devoid of lipidsynthesizing activities. Spatial separation of lipid biosynthetic steps and
lack of lipid synthesis in several cellular membranes necessitate an
efficient transfer of lipids from their site of synthesis to their proper
destination(s). The maintenance of organelle lipid profiles requires strict
coordination and regulation of biosynthetic and translocation processes.
scientific
20
Structural
biology
Inst. of Chemistry
+43-3163805423
8010
Graz
Heinrichstr. 28
Austria
membrane protein,
conjugation, T4SS
Institute of
Medical
Technologies
and Health
Mangement
Molecular
Biology and
Biochemistry,
Joanneum
Research
++43316876
2103
8036
Graz
Auenbruggerplatz
20/3
Austria
acyl-coA, mass
spectrometry,
interstitial fluid analysis
Medical University +433163804
Graz
200
A-8010
Graz
Harrachgasse 21
Austria
cholesterol
metabolism, nuclear
receptors,
atherosclerosis
IMB
Biochemistry Yeast Genetics
Group
University of Graz
++43 316
380 5487
A8010
Graz
Schubertstr. 1
Austria
Institute of
Chemical
Technologies
and Analytics,
Bio and Polymer
Analysis
Core Facility for
Mass
Spectrometry
Vienna University
of Technology
++43 1
A-1060
58801 15160
Vienna
Getreidemarkt
9/164
Austria
ZMF/Medical
University Graz
++43 (316)
385-73005
Graz
Stiftingtalstrasse
24
Austria
8010
Specific aspects currently studied are the assembly of lipids into
mitochondrial membranes and lipid homeostasis in this compartment,
and dynamics of neutral lipid storage in lipid particles. Recently we
started to extend our studies to organelles of Pichia pastoris as a basis
for future research of protein expression in this biotechnologically
important yeast. Enzymes and other proteins involved in the above
mentioned processes are investigated using biochemical, cell biological
and molecular biological methods.
We are working on the characterization of a typ IV-like secretion system scientific
(T4SS) from Gram positive bacteria. The system is encoded on the
resistance plasmid pIP501. The tra region shows a modular organization
and contains 15 ORFs, of which several have been predicted to encode
for transmembrane or membrane asociated proteins. Our aim is the
structure elucidation of the components essential for the conjugative
DNA transfer.
analysis methods based on GC-MS, HPLC-MS^2 and nano-HPLC-MS^2 scientific
analysis of interstitial fluid of humans and animals method validation
according to GLP guidelines
Novel and already identified genes involved in lipid homeaostasis of
different organs including liver, intestine, endothelial cells,
macrophages-foam cells. Cholesterol efflux and cholesteryl ester
hydrolases. Pathophysiology of athersoclerosis. Lipid metabolism in
gneral. Lipidome of human plasma in health and diseases. Lipidome of
enterocytes and cardiomycytes .
yeast, fatty acid,
Lipid metabolism and membrane assembly in yeast, yeast as a model of
membrane and lipid
lipid-associated disorders, regulation of fatty acid, triglyceride and
imaging
phospholipid metabolism, high-resolution microscopy, implementation of
novel imaging methods to investigate membrane and organelle stucture
and dynamics in yeast, yeast lipidomics
MALDi, ESI, multistage Characterisation of lipid pattern in human blood during dietary treatment
MS, plant lipids,
Characterisation of lipid pattern of industrial (as a renewable source of
human blood
chemicals), pharamceutical and medical relevant plants Development of
ultrafast and structurespecific MALDI and ESI mass spectrometric
techniques for all classes of lipids
Mass Spectrometry,
FT-MS, Glycerolipids,
Sphingolipids
scientific
scientific
scientific
Development of mass spectrometric tools for the analysis of lipids
scientific
(neutral and polar glycerolipids, sphingolipids, cholesterols) and lipid
derived second messengers (e.g. eicosanoids) is a key research interest
of our facility. This is reflected by participation in center grants such as
SFB Lipotox and the technology based initiative Lipidomic Research
Center (LRC) Graz. Currently we develop an analytical platform based
on UPLC-FT-MS/MS for differential quantitation of the lipidome at the
level of molecular species. Finally these data should enable researchers
to establish up- and down regulated metabolic pathways for different
sets of samples. If it is needed to provide absolute quantitative data for
single species or a limited set of them, we have the possibility to quantify
21
them in a targeted approach with LC-MS/MS.
phytanic, pristanic,
Breakdown of fatty acids/derivatives via alpha-oxidation and betaceramide, sphingosine- oxidation, peroxisomal lipid metabolism, bioactive sphingolipid
phosphate
metabolism, mouse models related to peroxisomal disorders,
Div.
Pharmacology Dep. Mol. Cell
Biology
CMPG-PFI
K.U.Leuven
++32-16345801
B-3000
Leuven
Campus
Gasthuisberg,
Herestraat
Belgium
Katholieke
Universiteit
Leuven
++32 16 32
96 88
3001
Heverlee
Kasteelpark
Arenberg 20
Belgium
yeast, sphingolipid,
ESI-MS
Laboratory for
Experimental
Medicine and
Endocrinology
(LEGENDO)
Katholieke
Universiteit
Leuven
++32-16330533
3000
Leuven
Herestraat 49 bus
902
Belgium
cancer, lipid rafts,
protein acylation
Neuronal
Differentiation
Unit
VIB and Catholic
University of
Leuven
+32-16330526
3000
Leuven
Herestraat 49
Belgium
cholesterol lipid rafts
senescence
Laboratório de
Bioquímica e
Biologia Celular
de Lipídios
Departamento de
Bioquímica ICBSUniversidade
Federal do rio
Grande do Sul
Hospital for Sick
Children
55-51-33-16- 90.03555-50
003
Porto Alegre
Rua Ramiro
Barcelos 2600anexo
Brazil
shingolipids,
gangliosides,
adipogenesis,
lipogenesis
1-416-8135919
M5G1X
8
Toronto
555 University
Ave.
Canada
sulfatide,
galactosylceramide,
glycosynapse
University of
Alberta
1-780-4922963
T6G
2S2
Edmonton
328 Heritage
Canada
Medical Research
Centre
atherosclerosis,
obesity,
neurodegeneration,
lipids
Research
Institute, Boggs
Laboratory
CIHR Group in
Molecular and
Cell Biology of
Lipids
scientific
We are interested in sphingolipidomics in yeast. Using ESI-MS, we
have optimized a method to determine and relatively quantify the three
different classes of complex inositolphosphoryl-containing sphingolipids
[i.e. IPC (inositolphosphoryl ceramide), MIPC (mannosyl
inositolphosphoryl ceramide) and M(IP)2C (mannosyl
diinositolphosphoryl ceramide] in S. cerevisiae [Aerts et al., 2006, FEBS
Letters, 580(7):1903-7]. We are interested in technologies enabling
quantification of sphingoid bases and ceramides in yeast membranes.
Our team is interested in the metabolic changes in cancer cells versus
normal cells, and particularly in the marked increase in lipogenesis that
is observed in nearly all cancer types. We 1. study the mechanisms that
underly this increase (imvolvement of oncogenes, steroid hormones,...),
2. We examine the impact of these chnages on lipid profiles, lipid rafts
and lipid-modified proteins, 3. We investigate the consequences of these
changes for cancer cell biology (growth, metastasis,...), and 4. We
explore the potential clinical applications (diagnosis, therapy).
We are interested in analysing the role of cholesterol in the cell
senescence/survival process. We postulate that cholesterol reduction in
membrane lipid rafts it might be a central effector in survival of
senescent neurons, implying that brain cholesterol regulation could be
crucial for death/survival equilibrium durin brain aging.
- Sphingolipid metabolism and their cellular functions in central nervous
system and hematopoietic differentiation. - Adipogenesis and
lipogenesis.
scientific
scientific
scientific
scientific
Structural organization of glycosphingolipids and effect of length of fatty scientific
acid chain and hydroxylation of fatty acid, Lipid rafts and membrane
domains in myelin and oligodendrocytes and role in signalling, Trans
interactions between glycosphingolipid head groups and formation of a
glycosynapse between apposed cell membranes, Role of
phosphatidylinositides.
Goals of our CIHR Group in Molecular and Cell Biology of Lipids (MCBL) scientific
* To enhance the knowledge and understanding of the metabolism,
function and transport of mammalian lipids, lipid biosynthetic enzymes
and transport proteins, and the regulation of the genes that encode
these proteins * To facilitate the translation of discoveries into potential
diagnostics and treatments of human diseases * To provide
fundamental knowledge that will improve the health of Canadians and
peoples throughout the world * To provide an environment that will
enhance scholarly and scientific endeavors. The principal investigators
* Luis B. Agellon, PhD, Associate Professor of Biochemistry * Gordon
22
Lab 305
Biochemistry
and Nutrition
Group,
Biocentrum-DTU
Institute of
Genetics and
Developmental
Biology, Chinese
Academy of
Sciences
The Technical
University of
Denmark
86-1064889783
100101
Beijing
Datun
Road,Chaoyang
District
China
+45
45252744
2800
Lyngby
Building 224, DTU Denmark
lipid, gene cloning,
gene function
characterization
LC/MS, animal
experimental facilities,
GC, TLC
A. Francis, MD, Associate Professor of Medicine * Richard Lehner,
PhD, Associate Professor of Pediatrics and Cell Biology * Dennis E.
Vance, PhD, Professor of Biochemistry * Jean E. Vance, PhD,
Professor of Medicine Our research program The MCBL Group research
program is organized into four major themes: * molecular regulation of
genes involved in lipid homeostasis * biochemistry of lipid-protein
interactions * lipid compartmentalization and intracellular trafficking *
lipid homeostasis in murine models The current major research projects
are: * regulation of phosphatidylcholine metabolism * molecular and
cell biology of phosphatidylserine metabolism * triacylglycerol
synthesis * role of triacylglycerol hydrolase in triacylglycerol
metabolism * function and metabolism of sterols in the liver *
metabolism of lipids in the enterohepatic circulation * cellular lipid
efflux and HDL formation * lipid homeostasis in neurons Our Core
Resources Members of the MCBL Group contribute specific technical
expertise and newly developed technologies which are shared with other
projects and laboratories through our Core resources. * The Core Cell
Culture Laboratory provides access to a variety of cell lines (including
various derivatives of McArdle RH7777 cells expressing recombinant
forms of enzymes important in the metabolism of lipids), and technical
support for immunofluorescence and confocal microscopy studies. *
The Core Metabolism and Physiology Laboratory provides access to
genetically-modified mouse strains used by investigators in the MCBL
Group. This laboratory also provides access to primary cell cultures, in
conjunction with the Core Cell Culture Laboratory, and technical support
for surgical procedures. * The Core Lipid Analysis Laboratory provides
access to current, standardized and validated analytical procedures, and
technical support for the detection and analysis of lipids and their
metabolites. * The Core Training Program provides opportunities for
qualified postdoctoral fellows and graduate students to participate in our
integrated research program.
I am interested in improvement of oilcrops, lipid synthesis related gene
scientific
cloning and gene function characterization.
Research areas/competencies related with lipidomics: Identification and scientific
quantification of triacylglycerols and phospholipids by LC/MS, separation
and quantification of different phospholipids and lipid classes by NPHPLC, separation and quantification of triacylglycerol molecular species
by RP-HPLC, separation and purification of lipids by TLC and SPE,
analysis of fatty acid profiles by GC, analysis of lipid regiostructures by
partial degradation (Grignard degradation for triacylglycerols &
phospholipase degradation for phospholipids) followed with GC, GC/MS,
LC/MS, analysis of plasma lipids by Cobas Mira, quantification of
cholesterol in different lipoproteins using HPLC. Other research
activities: Lipid absorption and metabolism, application of stable isotope
techniques and GC/C/IRMS in metabolic studies, effect of dietary fats on
23
University of
Helsinki
Helsinki
Biophysics &
Biomembrane
Group
Department of
Medicine
Institute of
Biomedicine
+358 50 563
6899
+358-919125400
00020
Helsinki
PO Box 700
Finland
liver insulin marker
FINHelsinki
00014
Universi
ty of
Helsinki
P.O.
Helsinki
Box 63
POB 63
Finland
lipids, drugs, proteins,
amyloids
Haartmaninkatu
8/P.O. Box 63
Finland
Lipid-protein
interactions, Mass
spectrometry, Ion
channels, Fatty acids
mass-spectrometry,
trafficking,
membrane,phospholip
ase
mitochondria,
peroxisomes, structural
enzymology
systems biology, early
markers,
metabolomics,
phenotype
characterization
Institute of
University of
Biomedicine/Käk Helsinki
elä Group
++358-919125409
Institute of
Biomedicine/So
merharju group
University of
Helsinki
358-919125410
00014
Helsinki
Haartmaninkatu
8/PL 63
Finland
Department of
Biochemisty
University of Oulu
+358-8-553
1150
FIN90014
Oulu
POB 3000
Finland
Quantitative
Biology and
Bioinformatics
VTT Technical
Research Centre
of Finland
++358-20720-4491
FIN02044
VTT
Espoo
Tietotie 2, P.O.
Box 1500
Finland
Biological
Chemistry
Laboratory
Biological
Chemistry
LaboratoryNation
al Institute for
Agronomic
Research (INRA)
++33-130815474
78850
Thiverval
Grignon
BP 1
France
oil, oil bodies, A
thaliana, Y lipolytica, B
napus
learning ability and visual function, bioavailability of n-3 fatty acids,
applying enzymatic technology in studies on lipid modification including
structured triacylglycerols and structured phospholipids, studies on new
dietary lipids such as diacylglycerols and conjugated linoleic acids,
interactions of lipids with other food components, lipoprotein oxidation,
development of analytical methods, especially emphasized on
separation techniques and mass spectrometry for analysis of lipids and
lipid soluble vitamins, qualitative and quantitative analysis of
carbohydrates. We possess extensive expertise in lipid biochemistry and
lipid analysis, and we have the following facilities: Analytical instruments
for lipid analysis of food and biological systems (GC, HPLC, TLC,
GC/MS, LC/MS, GC/C/IRMS, Cobas Mira, Spectrophotometer) Animal
experimental facilities
Profiling of human serum in individuals with a fatty vs no-fatty liver in
scientific
collaboration woth Matej Oresic.
lipid phase behavior, bioactive lipids, lipid-protein interactions, drug-lipid scientific
interactions, cationic lipids in gene delivery, lateral organization of
biomembranes
-Interactions of lipid membrane and membrane proteins/ion channels Cellular metabolism of lipids and fatty acids -Mass spectrometry of lipids
scientific
1)Lipid trafficking 2)Regulation of lipid composition of cells (lipid
homeostasis) 3)Mode of action of phospholipases 4)Membrane
structure, expecially lateral distribution of lipids 5)Atherosklerosis and
other lipid related diseases 6)Development of MS tools for lipid analysis
Lipids and metabolic disorders, yeast lipids, fatty acid synthesis,
membrane lipidomics, lipid metabolism, lipid metabolizing enzymes,
scientific
scientific
Animal model phenotype characterization using lipid (and general
scientific
metabolite) profiling approaches - Bioinformatics method for integration
of lipid profile information from mass spectrometry based approches with
other levels of data such as gene expression and protein profiles Clinical applications of lipidomics for early disease detection and
disease progression studies, focus on diabetes
We are interested in understanding the biology of oil bodies, with special scientific
emphasis on assembly and degradation. We are currently studying two
organisms: - A. thaliana, a model for for oil crop. -- Y. lipolytica, yeast
capable to use and transform lipids. We combine a multiscale approach
going from: -the identification of oil bodies proteins (proteomics,
enzymatic studies, immunochemistry) --the study of isolated proteins
(purified of recombined) in solution (enzymology, interfacial properties
using hanging drop methods), or within model interfaces (Langmuir
balance experiment) --- the study of proteins within complete organelle
(from wt or mutants organisms) ----the study of wt and mutant model
organism. ----- transcriptomic analysis of yeasts genes expressed or
24
INSERM-538
(biomembranes)
CHU SaintAntoine
33-0140011340
75571
PARIS 12°
27 rue Chaligny
France
Laboratory of
Enzymology at
Interfaces and
Physiology of
Lipolysis
CNRS
33 4 91 16
41 34
13402
Marseille
cedex 20
31 chemin Joseph France
Aiguier
CIML
CNRS INSERM
33
491269404
13288
marseille
parc sxcientific de
luminy
France
Laboratoire de
Biogenèse
Membranaire
CNRS UMR 5200
Université V.
Segalen
Bordeaux 2
+33 (0)5 57
57 10 45
33076
Bordeaux
cedex
146 Rue Léo
Saignat
France
membrane,
microdomains, traffic,
phospholipids
lipases, TLC-FID,
monolayers
abc transporters,
abca1, malaria,
flippase
Plant lipids, waxes,
fatty acids, GC, Lipid
analysis
repressed upon shift from glucose to oleic acid medium. -Our goals are
- to identify all protein component involved in oil bodies biogenesis -- to
understand their role and identify their activity ---to study the influence
of oil body protein composition on lipid content (nature, amount)
Principal collaborations: -Within France Dr. M Miquel, Dr. L Lepiniec,
Seed Biology Laboratory, INRA, Versailles: Molecular biology of A.
thaliana seeds, Dr JM Nicaud Molecular Genetic Laboratory, INRA
CNRS, Grignon: Molecular biology of Y. lipolytica. Dr. M Axelos: BIA
Laboratory, INRA, Nantes. Interfacial methods -Within European Union
Prof. G Daum, Dr K Athensdaedt, technical University, Graz: Lipid
particles from yeast Dr. S Papanikolaou, Agronomic University of
Athens, Biotechnology of Oleaginous yeast
Membrane heterogeneity and polarized cellular traffic. Cholesterol and
scientific
sphingolipids enriched membrane microdomains Rotavirus traffic in
Caco-2 cells. Synthesis of bile phospholipids
Lipolytic enzymes and their use for the characterization lipids Production scientific
facilities, purification and kinetic characterization of several recombinan t
lipases, including gastric and pancreatic lipases, pancreatic lipaserelated proteins, hormone-sensitive lipase, microbial lipases, Plant
phospholipase D, phospholipase A2, phospholipase A1, galactolipase
Use of thin-layer chromatography coupled to flame ionization detection
(TLC-FID, Iatroscan) for quantitative measurement of neutral and polar
lipid species and their lipolysis products. Use of the monomolecular film
technique for studying lipid-protein intercations and lipolytic enzymes
kinetics. This technique allows measurement of lipolytic activity with very
low amounts of substrate (g)and is therefore suitable for rare lipids.
lipid transport /flip by abac transporters of the A class lipidomic analysis scientific
of microparticles during malaria infection
The Membrane Biogenesis Laboratory (MBL) has a long history of
research in membrane biogenesis and lipid biosynthesis. This lab is
internationally known and has been recently chosen to organise the
International Plant Lipid Symposium in 2008. The aims of the research
concern the identification and the characterisation of the genes involved
in the regulation of lipid metabolism. Using Arabidopsis thaliana as the
main plant model, the topics currently developed in the unit are: i/
research project I: Genomics of epicuticular wax metabolism. The
objective is to characterise the genes involved in the biosynthesis of
epicuticular waxes in order to understand the regulatory mechanisms of
the expression of genes, which control the composition of the wax layer
in response to various stress conditions. ii/ research project II:
Membrane biogenesis and homeostasis. This project aims to
characterise the mechanisms controlling the membrane lipid
composition, the lipid transfer between organelles and membranes, and
the lipid composition of lipid rafts in relation to hormonal signalling. iii/
research project III: SNAREs, lipids and endomembrane dynamics. The
objective is to study the role of SNAREs in membrane dynamics
between the ER and the Golgi apparatus in the secretory pathway
25
scientific
leading to the biogenesis of plasmalemma and the formation of lipid rafts
through the secretory pathway. The MBL is one unit of the IFR 103
Integrative Biology which involves 6 labs covering the different areas of
plant research (pathogenesis, biotechnology, agronomy), and
technological platforms (imaging, lipidomic, metabolism, transcriptome).
The MBL has in charge the lipidomic platform which consist of 3 GLC,1
GC/-MS 1 densitometer, 1 phosphor imager and 2 TLC spotters. This
platform allows lipid and fatty acid analysis and lipid metabolism studies.
phospholipase A2, lipid Eleven genes coding for secreted phospholipases A2 (sPLA2s, 14-18
scientific
mediator, cancer,
kDa) and 2 types of receptors (M and N) have now been identified in
inflammation
mammalian tissues. sPLA2s and their receptors are found in several
tissues, and their expression levels are increased in inflammation,
associated diseases, different types of cancer, and neurodegenerative
diseases. Although the biological functions of sPLA2s and their
receptors are still ill-defined, some sPLA2s are mitogenic and/or
apoptotic, pro-inflammatory and/or pro-tumoral, but also anti-tumoral,
anti-bacterial, anti-viral, and anti-parasitic. At the molecular level,
sPLA2s are most likely bifunctional proteins, acting as both enzymes
and ligands for a variety of soluble and membrane proteins. sPLA2
enzymatic activity participates in the control of the rate-limiting step in
the production of lipid mediators such as prostaglandins and
leukotrienes which are involved in a myriad of biological effects. We
have previously identified for the first time the M and N type receptors
for sPLA2s using snake venom sPLA2s as ligands. More recently, we
have cloned 7 of the 11 known mammalian sPLA2s, and we have shown
that several of these enzymes are the natural ligands of the M-type
receptor, suggesting that mammalian sPLA2s are true ligands for
mammalian proteins. The main objective of our research program is to
determine the function of sPLA2s, while continuing their molecular
characterization. Our specific aims are : 1) To analyze the molecular
properties of sPLA2s and to develop key tools to determine sPLA2
function (recombinant production, native structure, crystallization,
enzymatic and binding properties, search of specific inhibitors,
characterization of receptors, transgenic mice), 2) To analyze the tissue
distribution of sPLA2s and their receptors in normal and pathological
states in relation with the following item, 3) To study the role of sPLA2s
in three major research areas : colorectal cancer, inflammation, and host
defense against viruses, bacteria, and parasites.
lipid rafts, eicosanoids, Protein-lipid interactions, proteomics and lipidomics of lipid
scientific
epithelium
microdomains, and their relevance in physiopathology of epithelial cells.
Regulation of inflammation in cystic fibrosis.
Institute of
molecular and
cellular
pharmacology
CNRS UMR6097
++33-(0)493957733
06560
Valbonne
Sophia Antipolis660 route des
lucioles
France
INSERM
U467/IFR94
Proteomics
Platform
French Institute
for Fats and Oils
(ITERG)
IMBL-Lipid
Signaling (UMR
585 INSERM /
Faculté de
Médecine René
Descartes Paris 5
+33 1 40 61
56 21
75015
Paris
156 rue de
Vaugirard
France
Industrial
Technical Center
+33 5 56 36
00 44
33600
PESSAC
rue Monge
France
nutrition bioavailability
cancer CHD
69621
Villeurbanne
20 Ave A.
Einstein
France
Signaling , Fatty acids , INSERM UMR 585 / INSA-Lyon is part of IMBL (Institute for
Lipid mediators ,
Multidisciplinary Biochemistry of Lipids). This UMR works on lipid
Analysis
signaling, in blood, vascular cells and adipocytes, in the context of
INSA-Lyon and
33-4-72 43
CNRS / INSERM / 82 40
Univ Lyon1
Nutritional impact of lipids (in relation to diseases : CHD, cancer,
obesity). Biomarkers studies (adipose tissue, plasma, erythrocytes).
industrial
scientific
26
INSA-Lyon)
INSERM U671
INSERM
++33142346
923
75006
Paris
15 rue de l ecole
de medecine
France
adipocyte obesity lipid
droplet
INSERM U586
(Obesity
Research Unit)
INSERM,
33-562172956
31432
Toulouse
CHU Rangueil
BP84225
France
lysophosphatidic acid,
autotaxin,
lysophospholipase D,
adipocyte,
IFR30 Lipid
Analysis
Platform
INSERM/Toulous
e Genopole
33
(0)56177941
4
31024
Toulouse
CPTP Bat C,
Hopital Purpan
BP3028
France
neutral lipid, ceramide,
bile acid, phytosterol
aging, atherosclerosis, diabetes and obesity. Lipids of interest are
membrane phospholipids as reservoirs of polyunsaturated fatty acids
which serve as precursors of eicosanoids and docosanoids. The
production of those oxygenated metabolites through cyclooxygenase
and lipoxygenase pathways is the main focus. The role of oxidative
stress/lipid peroxidation in those pathways as well as their control by
polyunsaturated fatty acids of nutritional value are of special interest.
The sphingomyelin / ceramide / sphingosine / sphingosine-1-phosphate
is also taken into consideration.
Synopsis of current research interest in the field of lipid droplet biology:
scientific
We are working in the field of metabolic diseases associated with
obesity in an INSERM unit in Paris (UMR 671). In these diseases,
ectopic lipid storage is a key deleterious event at the whole body level,
associated with grave metabolic complications. In this regard, we are
interested in lipid trafficking in adipose cells specialized in the storage of
fat. Caveolins have been found at the surface of lipid droplets, but their
functional role in the dynamics of fat storage remains unknown. Since
caveolins are highly expressed in adipocytes, and caveolin-KO mice
have revealed a striking inability to store fatty acids from extracellular
sources into their adipose tissue, we focused our research on the role of
caveolins in lipid homeostasis. In a recently published paper (Le Lay et
al., Traffic 2006), we have shown that adipocyte lipid droplet
composition was dependent on caveolin expression and that exogenous
cholesterol was able to induce caveolin targetting to these lipid droplets
through a process sharing many features in common with caveolar
endocytosis. We are now interesting in elucidating the dynamic
relationship between cell surface and lipid droplets through caveolin
trafficking, and identifying the adipocyte lipid species that can be stored
through a caveolin dependent pathway. In this line, our future projects
deal with the functional role of caveolins in lipid targeting to the fat
storage compartment, the lipid droplet.
My group is interested in the regulations and the biological
scientific
consequences of the release of lysophosphatidic acid (LPA) by
adipocytes. We are particularly interrested in a lysophospholipase D
(autotaxin) which appears to be the main source of LPA release by
adipocytes but which could also be involved in synthesis of several other
bioactive phospholipids. ATX expressio is adipose tissue is upregulated in association with insulin-resistance and type 2 diabetes. In
paralelle, we are also interrested in understanding the transduction
pathways (particularly the receptors) involved in LPA actions on the
different cell types surrounding adipocytes in the adipose tissue
including preadipocytes, macrophages, endothelial cells.
Our Lipid Analysis Platform belongs to the French Nationale Institute for scientific
Health and Medical Research (INSERM). It is located in the Federative
Research Institute (IFR30) in Toulouse, France and is associated to the
Toulouse Genopole. The platform created in 2003 proposes a number of
lipid analysis using chromatographic methods, either adapted from the
litterature or original. We are equiped with 3 gas chromatographs (GC)
with FID detection, one GC coupled to a mass spectrometer and one
27
Microbiology
and Molecular
Genetic
Laboratory,
UMR2585
National Institute
++33
for Agronomic
130815450
Research (INRA)
and National
Centre for
Scientific
Research (CNRS)
F78850
ThivervalGrignon
CBAI B.P. 01
France
Cyberlipid
Center
Nutrition,
Croissance et
Cancer,
INSERM E211
Private
3304674143
65
Université
+33 (0)247
François Rabelais 366179
34090
Montpellier
France
37044
TOURS
2 rue du colonel
Marchand
CHU Bretonneau
University of
Bourgogne
University
21000
DIJON
6 Bd Gabriel
France
+33 3 80 39
63 12
France
liquid chromatograph (HPLC) coupled to a light scattering detector
(LSD). We propose qualitative and quantitative analysis of lipid
molecules following extraction from micro-samples (biological fluids,
tissues, cells). We thus have access to the molecular species of the
following lipids : - Neutral lipids including cholesterol and related sterols,
diacylglycerol, cholesterol ester and triacylglycerol (on the basis of
carbon number) - Free or total fatty acids - Sphingomyelines and usual
ceramides - Phytosterols - Bilary acids (conjugated or free) - We are in
progress to propose in the near future the analysis of major
phospholipids classes, galactolipids and cerebrosides by HPLC-LSD.
Analysis are proposed on the basis of a price per sample. Collaboration
on specific projects is opened. Present ongoing projects include the
fields of intestinal lipid absorption, cancer and apoptosis, lipoprotein
metabolism, nervous system functionnning, lipid transduction
yarrowia lipolytica, lipid We are interested in understanding the degradation of hydrophobic
accumulation, genetic
substrates (alkanes, fatty acids, oils) by the oleaginous yeast Yarrowia
lipolytica. We combine a multistage approach going from: transcriptional analysis of gene expression during growth on
hydrophobic substrates using Y lipolytica micro array, - the identification
of peroxisomal and oil bodies proteins (proteomics), - the study of wildtype and mutants affected in HS utilisation, in beta oxidation, in lipid
accumulation, ... Our goals are to identified genes involved in lipid
accumulation (functional analysis, nature and amount of fatty acid
accumulated), lipid and HS transport into different cell organelles.
Principal collaboration: - within France Dr T Chardot, Biological
Chemistry, INRA, Grignon (biochemistry of lipid bodies), Prof N Latruffe,
Burgundy University, Dijon (peroxisome), MC C Molina-Jouve, INSA,
Toulouse (Biotechnology of oleaginous yeast), - within European Union:
Prof G Daum, Dr Athensdaedt, Technical University, Graz (lipid particles
from yeast), Dr S Papanicolaou, Agronomic University of Athens
(Biotechnology of oleaginous yeast).
database internet lipids Construction and administration of an internet site devoted to all lipid
aspects www.cyberlipid.org which is yet cited in your links page
diet, cancer, lipidome,
In the field of cancer prevention through dietary lipids, we use the white
PUFA, CLA
adipose tissue lipid composition as a qualitative indicator of past dietary
intake of lipids. Through a reappraisal of white adipose tissue data
obtained recently in human and animal models, we individualized a
composite lipid profile associated with a low risk of breast cancer. This
profile has led us to elaborate a composite index indicative of the risk of
breast cancer. This biomarker offers the opportunity to quantify the part
due to modifiable dietary factors in the risk of breast cancer and
complements the currently known risk factors for breast cancer.
Bougnoux P, Giraudeau B, Couet C. Diet, cancer and the lipidome.
Cancer Epidemiology, Biomarkers & Prevention 2006, 15(3):416-21.
Diacylglycerols, MAP
Dietary polyunsaturated fatty acids (PUFA) have been classified into two
kinases, T cells,
categories, belonging to n-6 and n-3 families. These fatty acids are
Macrosomia
indispensable for animal cell to maintain its structure, fluidity and
function. The polyunsaturated fatty acids (PUPA) of n-3 family have
28
scientific
scientific
scientific
scientific
Applied
Biosystems
AkdÄ
Julius-v-SachsInstitute for
Biosciences,
Pharmaceutical
Biology
Eicosanoids and
Tumor
Development
Applera
Deutschland
GmbH
Arzneimittelkomm
ission der
deutschen
Ärzteschaft
Biocenter,
University of
Wuerzburg
+496151967
05231
64293
Darmstadt
Frankfurter
Strasse 129B
Germany
++49304004
56500
10623
Berlin
Herbert-LewinPlatz 1
Germany
++49-931888-6160
D97082
Wuerzburg
Julius-von-SachsPlatz 2
Germany
D
69120
Heidelberg
Im Neuenheimer
Feld 280
Germany
Deutsches
49 6221
Krebsforschungsz 424506
entrum
SOP, MS, LCMS,
funding, TaqMan
Assays
Clinical effects of lipid
lowering, outcome
trials, statins, safety of
statins
plant oxylipins,
isoprostanes
Lipoxygenase,
ichthyosis, epidermal
barrier
been considered as authentic immunosuppressors, however, their
mechanisms of action in T-cell activation have not been well elucidated.
T-cell activation involves a series of complex mechanisms from
membrane receptor to gene transcription via the second messenger
cascades. The main theme of our research is to elucidate the role of
dietary PUPA of n-3 family in human T-cells activation which might be
involved in the pathology of several diseases like diabetes and obesity.
We are studying the interaction of these fatty acids with the second
messenger cascade / cell signalling (MAP kinases, calcium signalling,
protein kinases C & diacylglycerol).
Co-Developement of Gene Expression Assays Standard Operating
Procedures for MS Lipid Analysis Business developement / Product &
Service developement in Lipidomics
Clinical effects of lipid lowering, outcome trials, statins, safety of statins
industrial
scientific
We are interested in the functional analysis of plant lipids. There is a
scientific
long track record on plant oxylipins of the jasmonate type. However
more recently we came interested in oxidized lipids derived from nonenzymatic oxidation of plant membrane lipids. The current focus is on
hydroxy fatty acids and prostaglandin-like plant isoprostanes termed
phytoprostanes. Formation of these compounds in response to
environmental stress conditions is intensively been investigated in
cyanobacteria and plants. It has been shown by our group that oxidized
lipids (including phytoprostanes)represent archetype signals that induce
detoxification and defence responses in plants and cyanobacteria.
Moreover oxidized plant lipids also have an pronounced effect on
mammalian immune cells. In the future, a comprehensive analysis of
plant oxidized lipids and their biological effects in vivo (cyanobacteria,
plants) will be performed. The lab is equipped with three GC-MS (sector
field MS and quadrupol MS instruments). More recently, an Nano-ESIHPLC-Ion-Trap MS instrument for plant proteome analysis and a HPLCTriple Quadrupol MS for plant lipidomics have been purchased.
The long-term objective of the research group is to substantially
scientific
contribute to the understanding of the role of eicosanoids, which perform
important tasks as local signals coordinating the concerted activities of
different cell types in tissues under normal and disease conditions with a
special focus on cancer. As autocoids the biological activities of
eicosanoids are primarily determined by their biosynthesis via the
cyclooxygenase (COX) and the lipoxygenase (LOX) pathways.
Accordingly, the expression and activity of COX and LOX are strictly
regulated under normal conditions, transiently up- or down-regulated
during irritation and tissue regeneration, and permanently deregulated
during carcinogenesis in both humans and mice. The group’s
experimental strategy is to establish expression and activity profiles of
individual LOX in mouse and human biopsies in order to bridge the
animal data with clinical investigations. The experimental models used
29
Deutsche
Gesellschaft für
Proteomforschu
ng
department of
cellular and
molecular
pathology
DGPF
089-1897
9007
82152
Martinsried
Am Klopferspitz
18
Germany
Proteomics, proteome
research
DKFZ(German
Cancer Research
Center)
49-6221424350
D69120
Heidelberg
Im Neuenheimer
Feld 280
Germany
Tübingen
Calwerstr. 7
Germany
glucosylceramide
synthase,cerebrosides
ulfotransferase,cellspecific
deficiency,fertilify
Phospholipids, in vivo
metabolism, D9choline, human
Dept. of
Eberhard Karls
Neonatology,
Universität
Lipid Metabolism Tübingen
and
Developmental
Physiology
Group
#49-(0)7071- 72076
29-86377
for functional studies include phorbol ester-irritated or wounded mouse
skin, the two-stage skin carcinogenesis approach, and transgenic
mouse lines with targeted over-expression or deletion LOX genes in skin
epidermis or other epithelia in vivo and epithelial cells in vitro. Our
current work is focussed on analyses of molecular mechanisms involved
in the functions of epidermis-type LOX in tissue homeostasis and
disease-related deregulation of eicosanoid metabolism.
Proteomics
scientific
Glycosphingolipid and immune function Glycosphingolipid and
transgene mice
scientific
The Lipid Metabolism and Developmental Physiology Group focuses on scientific
clinical as well as on developmental and comparative aspects of the
metabolism of individual (phospho)lipid molecular species. Main topics
are: (1) the metabolism of pulmonary phospho- and other lipids, together
with that of their hydrophobic and hydrophilic proteins, in relation to
pulmonary maturation, ante- and perinatal diseases and the
development of bronchopulmonary dysplasia. (2) the function of
individual and characteristic phospholipid molecular species with respect
to air-liquid interface function under dynamic conditions and to
immunological differentiation of immune-competent cells in terminal lung
tissues. (3) the metabolism and secretion of hepatic phospholipids and
their role in the supply of peripheral organs with lipids and lipid
precursors during postnatal development and at different clinical
settings, where liver function is compromised. To investigate these
issues we have performed a lipidomic approach to individual
phospholipid molecular species composition and metabolism of
pulmonary surfactant systems. This includes investigation across a wide
spectrum of vertebrate species in relation to development and their
differing parameters of pulmonary physiology, air-liquid interface
dynamics and parenchymal structures (avian air capillaries, mammalian
sacculi, alveoli and airways). Furthermore, it includes investigation of
functional characteristics of individual phospholipid components for
phagocyte differentiation and function as well as for surface tension
function. We have furthermore studied the relation of pulmonary
surfactant molecular composition to extrapulmonary interfaces, like that
of the hydrophobic gastric barrier. To investigate phospholipid
metabolism under different clinical conditions and during postnatal
development we have developed and established strategies using stable
isotope labelled precursors in vivo in human subjects as well as in
animal models, combined with electrospray-ionization tandem mass
spectrometric (ESI-MS/MS) analysis of hydrophilic phospholipid
precursors and their synthesis products. We have furthermore
established animal models suitable for the investigation of inflammatory
30
Albrecht-vonHaller-Institute
for Plant
Sciences/Plant
Biochemistry
Georg-AugustUniversity
++49-55139-5743
37077
Goettingen
Justus-von-Liebig
Weg 11
Germany
Plant lipid metabolism,
lipid peroxidation
processes, oxylipins,
&#946,-oxidation
Albrecht-vonHaller-Institute
for Plant
Sciences/Plant
Biochemistry
Georg-AugustUniversity
++49-55139-5743
37077
Goettingen
Justus-von-Liebig
Weg 11
Germany
Plant lipid metabolism,
lipid peroxidation
processes, oxylipins,
&#946,-oxidation
Department of
Macromolecular
Structure
Research
Institute of
Biology/Molecul
GKSS Research
Center
++49-415287-1291
21502
Geesthacht
Max-Planck-Str. 1
Germany
peptide antibiotics,
membrane biophysics,
scattering
HumboldtUniversity Berlin
49 (0) 30
2093 8860
D10115
Berlin
Invalidenstr. 42
Germany
lipid, transport, fusion,
virus
and hereditary diseases and during development, where changes of
pulmonary and/or hepatic phospholipid metabolism as well as that of
lipid-derived mediators is prominent (bronchopulmonary dysplasia,
hyperoxic lung injury, cystic fibrosis).
A central aspect in lipid metabolism is lipid peroxidation. It is common to
all biological systems, both appearing in developmentally and
environmentally regulated processes. Products are hydroperoxy
polyunsaturated fatty acids and metabolites derived there from
collectively named oxylipins or eicosanoids in mammals. They may
either originate from chemical oxidation or are synthesized mainly by the
action of various highly specialized forms of lipoxygenases. We are
analyzing the physiological function of oxylipins during the degradation
of storage lipids in oilseed seedlings as well as the involvement of
lipoxygenases in the interaction of plants with pathogenic fungi and
bacteria. Another research focus is the ana- and catabolism of lipid
peroxides by analyzing the biosynthesis of hydro(pero)xy polyenoic fatty
acids, aldehydes, ketols and divinyl ethers by combined biochemical and
analytical methods as well as by using a metabolomic approach (oxylipin
profiling). The use of plants as bioreactors for biotechnological purposes
is addressed by isolating new fatty acid desaturases, acyl transferases
and double bond isomerases in order to introduce new functional groups
in seed oils for nutritional and industrial purposes. In order to identfy
bottle necks within these projects we use lipid profiling methods.
A central aspect in lipid metabolism is lipid peroxidation. It is common to
all biological systems, both appearing in developmentally and
environmentally regulated processes. Products are hydroperoxy
polyunsaturated fatty acids and metabolites derived there from
collectively named oxylipins or eicosanoids in mammals. They may
either originate from chemical oxidation or are synthesized mainly by the
action of various highly specialized forms of lipoxygenases. We are
analyzing the physiological function of oxylipins during the degradation
of storage lipids in oilseed seedlings as well as the involvement of
lipoxygenases in the interaction of plants with pathogenic fungi and
bacteria. Another research focus is the ana- and catabolism of lipid
peroxides by analyzing the biosynthesis of hydro(pero)xy polyenoic fatty
acids, aldehydes, ketols and divinyl ethers by combined biochemical and
analytical methods as well as by using a metabolomic approach (oxylipin
profiling). The use of plants as bioreactors for biotechnological purposes
is addressed by isolating new fatty acid desaturases, acyl transferases
and double bond isomerases in order to introduce new functional groups
in seed oils for nutritional and industrial purposes. In order to identfy
bottle necks within these projects we use lipid profiling methods.
interaction in or at membrane interfaces sensing of biophysical
membrane properties by proteins modification of biophysical membrane
properties by proteins, peptides or membrane active molecules peptide
antibiotics, viral infectivitity
The research is interdisciplinary ranging from structural biology,
molecular and cell biology, virology, spectroscopy to the design and
31
scientific
scientific
scientific
scientific
ar Biophysics
Microbial
Expression
Technology
LIPIDOMIX
GmbH
Walther-StraubInstitute
Institute for
Molecular
Enzyme
Technology,
Heinrich-Heine
University
Duesseldorf
LIPIDOMIX
GmbH
+492461612
947
52426
Juelich
Stetternicher
Forst
Germany
lipolytic enzymes,
biotechnology,
biodetergents
++49-3076778940
D13088
Berlin
Berliner Allee
261-269
Germany
eicosanoids,
phospholipids, HPLC,
mass spectrometry
LudwigMaximiliansUniversity
+49-8921807564
80336
Munich
Goethestr. 33
Germany
inflammation,
eicosanoids, obesity
synthesis of biomolecule analogues. The topics of basic and applied
research are protein-mediated fusion of membranes (viral fusion
proteins), virus budding, lipid-trafficking in eukaryotic cells, protein-lipid
interaction in membranes, and application of membranes in
nanobiotechnology. Cell Entry and Budding of Enveloped Viruses: A
focus of our research is an early step of cell entry of enveloped viruses.
Enveloped viruses as influenza virus or HIV fuse with respective
membranes to deliver their genome into the host cell. In our lab
enveloped viruses are employed to study the molecular mechanism of
protein-mediated virus-membrane fusion. We use various biophysical
and cell biology methods to follow directly the fusion process and to
identify structural intermediates mainly by fluorescence microscopy and
spectroscopy. To understand the functioning of viral fusion proteins,
specific mutants and chimeras of those proteins are created and probed
for their fusion activity. By strong collaboration with groups specialised in
electron microscopy and in image reconstruction, we identify the three
dimensional structure of complete viral fusion protein at conditions
typical for triggering membrane fusion. Another research focus is the
assembly and budding of enveloped viruses. We are interested in
mechanisms underlying local enrichment of viral components at the
budding site of the host membranes. Lipid-trafficking in eukaryotic cells:
Lipids play an essential role in various cellular processes including
signal transduction. Our specific interest is the protein-mediated
translocation of lipids across cellular membranes and their intracellular
transport to distinct organelles. Although indirect evidence already exists
the molecular identification of translocases pumping on the expense of
energy (specific) lipids across membranes and of flippases facilitating a
rapid, energy independent and unspecific movement of lipids in
membranes is still awaiting. We are very much interested in the
physiological relevance of those protein-mediated lipid transports and
their function(s), for example in cell genesis, apoptosis, fertilisation, cell
shape, exo- and endocytosis, in the enrichment of specific lipids in the
bile fluid as well as in the mechanism and consequences of the lipid
transport mediated by multi drug resistance proteins in tumor cells.
- Biochemistry and biotechnology with lipases, esterases,
scientific
phospholipases, thioesterases - Biodetergents, e. g. rhamnolipids from
Pseudomonas aeruginosa - Membrane processes in pathogenic
bacteria
Analytic of eicosanoid related substances like: Prostaglandins
Leukotrienes Hydroxy- and Epoxy fatty acids High throughput analytics
Replacement of immuno assays and: Fatty acids Phospholipids,
Sphingolipids Lipid profiling using: HPLC - Triple quad mass
spectrometry
Arachidonic acid metabolism, pharmacology of inflammation,
neurotransmitter, obesity
32
industrial
scientific
Max Planck
Institute of
Molecular Plant
Physiology/Plant
Lipid Group
Institute of
Medical Physics
and Biophysics
Max Planck
Society
++49-331567-8259
14476
Golm
Am Mühlenberg 1
Germany
galactolipid,
tocopherol,
phylloquinone
Biochemistry and Molecular Biology of Plant Lipids: Galactolipids,
Tocopherol, Phylloquinone, Chloroplast Lipids, Phospholipids, Fatty
Acids
scientific
Medical Faculty,
University of
Leipzig
++49-3419715733
04107
Leipzig
Härtelstrasse 1618
Germany
MALDI, 31P NMR,
Phospholipids,
Lysophospholipids
scientific
Institute for
RWTH Aachen
Biology I, Botany University
+49-(0)2418026641
52074
Aachen
Worringerweg 1
Germany
plant glycerolipid
isoprenoid
Institute for
Pathology,
University
Hospital Áachen
Kekulé Institut f.
Organische
Chemie und
Biochemie
RWTH-Aachen
++49241808
9729
52074
Aachen
Pauwelstr. 30
Germany
acyl-CoA, ceramide,
liver
We are using using MALDI-TOF mass spectrometry and 31P NMR
spectroscopy for the evaluation of physiologicallly relevant
phospholipids. Besides methodological work, we are particularly
interested in - the activity determination of phospholipases and
phosphoinositde kinase - the lipid / phospholipid composition of tissues
and body fluids - the changes of the lipid patterns under inflammatory
conditions (e.g. in rheumatoid arthritis and atherosclerosis) - the
changes of the lipid pattern of cells during differentaition
our research focuses on plant lipid metabolism with special interest in
formation/function of glycerolipids (glycerophospholipids) and
isoprenoids.
Functional characterisation of acyl-CoA synthetase and its products,
esp. in gastrointestinal tract and liver. Cellular localisation, concentration
and function of acyl-CoAs and lipids.
Universität Bonn
++49 228
735346
53121
Bonn
Gerhard-Domagk- Germany
Straße 1
Sphingolipids,
Glykosphingolipids,
Sphingolipidoses, Lipid
transfer proteins
Kekulé-Institute
for Organic
Chemistry and
Biochemistry
University Bonn
++49-228732703
53121
Bonn
Gerhard-Domagk- Germany
Str. 1
Department of
Gastroenterolog
y
University
Heidelberg
(+49) 6221
5638960
69120
Heidelberg
INF 410
Germany
Insitute of
Laboratory
Medicine,
Clinical
Chemistry and
Molecular
Diagnostics
Institute of
University
Hospital Leipzig
++49-3419722407
04103
Leipzig
Liebigstraße 27
Germany
sphingosine-1phosphate, ceramide1-phosphate,
neurodegeneration,
postoperative ileus.
Acyl-CoA synthetases,
Lipid droplets, Rafts,
inflammatory bowel
disease
phytosterols,
Sterol lipids: sterol metabolismn and atherosclerosis. Application of LCoxysterols, eicosanoids MS/MS for the measuerment of free and esterified phtyosterols,
lanosterol and cholesterol in serum and tissue. Eicosanoids: Evaluation
of a LC-MS/MS method for the measurements of eicosanoids as marker
of oxidative stress and inflammation.
University of
++49-221-
50931
Cologne
Joseph-
Germany
lipid droplets,
scientific
scientific
The research of Konrad Sandhoffs group focuses on sphingolipids and
scientific
sphingolipidoses. Metabolism of sphingolipids and glycosphingolipids, its
function, regulation and topology are studied in vitro at the cellular and
organismic level. Sphingolipid disorders are analyzed to clarify human
diseases and their molecular and cellular pathogenesis. At the
biochemical level, we synthesize sphingolipid probes and analogues to
analyze their molecular environment in the cells, and to characterize
enzymes and sphingolipid activator proteins involved in sphingolipid
metabolism and membrane digestion at lipid-water interphases. We
develop lipid transfer assays for the analysis of lipid transfer between
membranes by transfer proteins.
A)Sphingosine-1-phosphate signaling in terminally differentiated postscientific
mitotic neurons and its implications for the pathology of
neurodegenerative disorders. B)The pro-inflammatory functions of
sphingosine-1-phosphate and ceramide-1-phosphate and its
implications in postoperative ileus.
Acyl-CoA synthetases in fatty acid uptake Lipid droplet formation Lipid
scientific
rafts in fatty acid uptake Lipid rafts in intestinal inflammation Lipid MS
analysis of intestinal mucus
Our interest covers the analysis of lipid droplet associated proteins and
33
scientific
scientific
Biochemistry I
Cologne
4783656
Stelzmann-Str. 52
biosensor technology,
membrane protein
trafficking, endocytosis
Biocenter Klein
Flottbekand
Botanical
Garden/Plant
Physiology
Institut of
Nutrition
Institut for Food
Chemistry
University of
Hamburg
+49-(0)4042816-343
D22609
Hamburg
Ohnhorststr. 18
Germany
sphingolipid,
desaturase,
glycosyltransferase
University of jena
D07743
48149
Jena
Muenster
Dornburger Str.
24
Corrensstr. 49
Germany
University of
Muenster
++49-03641949610
+49 251 83
33867
Leibniz-Institute
of
Arteriosclerosis
Research
Leibniz-Institute
of
Arteriosclerosis
Research
Department of
Neurology
University of
Münster
+49 251
8356181
48149
Münster
Domagkstr. 3
Germany
University of
Münster
+49 251
8356181
48149
Münster
Domagkstr. 3
Germany
University of
Regensburg
+49 941
9410
93051
Regensburg
Universitätsstr. 84 Germany
Institute for
Clinical
Chemistry
University of
Regensburg
++49-941944-6201
93053
Regensburg
Franz-JosefStrauss-Allee 11
metabolism, CLA,
omega-3, sterol
HPLC-MS/MS
fumonisin sphingolipids
food ceramide
synthase
coronary heart
disease, biomarkers,
NMR, mass
spectrometry
coronary heart
disease, biomarkers,
NMR, mass
spectrometry
alpha-synuclein,
Parkinson,
Neurodegeneration,
synaptic plasticity
ABC Transporter, Lipid
Biomarker, Biobanking
Germany
Germany
their function in lipid turnover. Coming from the field of membrane
protein trafficking we apply cell based assay based on life cell imaging
and conventional confocal immunofluorescence techniques to localize
proteins. We further have a long-standing expertise in the analysis of
biomolecular interaction analysis using surface-plasmon-based
biosensors (BIAcore 3000, T-100). We analyse protein-protein
interactions as well as membrane protein interactions by this very
sensitive method.
Molecularbiology and biochemistry of plant and fungal lipids: Functional
characterizations of sterol-, sphingolipid and glycerol lipid-modifying
enzymes such as glycosyltransferases and desaturases/hydroxylases.
scientific
fatty acid metabolism, CLA, omega-3 LC-PUFA, sterol metabolism
scientific
HPLC-MS/MS analysis of sphingolipids Influence of fumonisins on the
sphingolipid metabolism
scientific
Research on biomarkers related to chd, stroke, Alzheimer disease risk.
Specific interest in technologies: H1-NMR, MS-MS, LC-MS, GC-MS.
scientific
Research on biomarkers related to chd, stroke, Alzheimer disease risk.
Specific interest in technologies: H1-NMR, MS-MS, LC-MS, GC-MS.
scientific
membrane microdomains and alpha-synuclein interaction in synaptic
vesicle dynamics and plasticity
scientific
The research of the institute is directed towards the molecular analysis
of metabolic and degenerative diseases. The central role of lipids in the
pathogenesis of these disorders is a the main focus of the Institute of
Clinical Chemistry. Our strategy combines basic research with clinical
association studies enabling a rapid transfer of newly identified
candidate markers from basic science to clinical diagnostics in order to
support prediction, prevention, and therapy of diseases. The clinical part
involves the Regensburg Diabetes Endpoint Prediction and Prevention
Study (REDEPPS), an interdisciplinary platform that enables the
establishment of large sample and data banks from patients with
different endpoints or co-morbidities of diabetes, such as coronary heart
disease, stroke, hypertension, obesity, nephropathy, retinopathy and
neuropathy. Candidate markers which are verified for their diagnostic
applicability in these cohorts are derived from basic research project
focusing on the regulation of lipid homeostasis and vesicular traffic as
related to disease pathogenesis. In this context the group has recently
identified the ATP binding cassette transporter ABCA1 as a major
scientific
34
Department of
Physiological
Chemistry
University of
Veterinary
Medicine
Hannover
++49-511953 8780
D30559
Hannover
Bünteweg 17
Germany
Laboratory of
Lipid Analysis
University of
Ioannina
+302651098367
451 10
Ioannina
Panepistemioupol
is
Greece
+36 30 320
97 69
4012
Debrecen
Nagyerdei Krt. 98
Hungary
+36-62-432048
H-6726
Szeged
36 1 215
6924/3610
1094
++36-52455866
4010
Laboratory of
Nutritional
Bioactivation
and Bioanalysis
Department of
Biochemistry and
Molecular
Biology,
University of
Debrecen
Laboratory of
Institute of
Molecular Stress Biochemistry,
Biology
Biological
Research Centre,
HUng. Acad. Sci.
Dept of Human
Semmelweis
Morphology and University
Developmental
Biology
Dept. Biophysics University of
and Cell Biology Debrecen
Lipid microdomains,
early detergentresistant membranes,
polarized protein
sorting, colitis
Lung surfactant,
apoptosis,
inflammation
regulator of HDL metabolism. The function of ABCA1 in the formation of
lipid microdomains and vesicular transport is subject of a current project
within the Transregional Collaborative Research Centre 6031 entitled
Membrane Microdomains and Their Role in Human Disease funded by
the DFG. The available technologies include high throughput genotyping
platforms and DNA-arrays for genetic analysis, protein analysis by
MALDI-TOF and the 2D-Gel Typhoon system, multicolour flow cytometry
and fluorescence imaging as well as tandem mass spectrometry and
NMR spectroscopy for lipids analysis. The methodological expertise of
the institute is complemented by the competence centre for fluorescent
bioanalysis (KFB) a public private partnership involving various institutes
of the University of Regensburg.
Membrane transport, polarized protein sorting: role of lipid
scientific
microdomains/detergent-resistant membranes, Intestinal membrane
topology in IBD
Analysis of lung surfactant lipids and their interaction with surfactant
proteins Lipids as substrates for phospholipases: metabolism, structural
studies, modeling Lipid modifications Lipids and signal transduction
Techniques in Lipid Analysis: TLC, gas chromatography, HPLC (normal
and reversed phase), Mass spectrometry (FAB, EI), Light scattering
HPLC MS-MS, retinoic HPLC MS-MS of retinoids, carotenoids and PUFA metabolites (HETEs,
acid, carotenoid, PUFA HODEs, eicosanoids).
metabolite
scientific
Temesvari krt. 62. Hungary
stress sensing, lipid
molecular species,
mass spectrometry,
rafts
Regulation of heat shock gene expression by the physical state, lipid
composition, mass spectrometry of lipids, microdomain organization of
membranes in different systems, the mechanism of thermoadaptation,
stress sensing and signaling, cellular thermosensors, etc.
scientific
Budapest
Tuzolto u. 58
Hungary
caveoae, lipid rafts
In my lab we are very much interested in caveolae, mainly the function
of these special lipid raft
scientific
Debrecen
Egyetem ter 1.
Hungary
P-glycoprotein, lipid
rafts, cytoskeletal
anchorage
We wish to understand the relationships between catalytic activity,
scientific
conformational changes and topological constraints of the ABC
transporter P-glycoprotein (Pgp), both in the context of multidrug
resistance (mdr) and its physiological roles, also to screen more
efficient/ selective Pgp modulators. We are currently focusing on the
localization of Pgp within and outside of lipid rafts, in conjunction with its
conformational and catalytic characteristics. Our experimental systems
include: (A) a novel flow-cytometric method to detect Pgp conformational
changes upon its interactions with modulators, based on antibody
competition, (B) a flow-cytometric platform for the measurement of raft-,
and cytoskeleton-association of cell-surface proteins, applicable for
35
scientific
Sackler School
of MedicineMinerva Center
Tel Aviv
University
972-36407842
69978
Tel Aviv
Levanon
Israel
fatty acid desaturases,
nutrition,metabolic
syndrome
Dept of
Biological
Chemistry
Weizmann
Institute
++972-89342704
76100
Rehovot
1 Herzl Street
Israel
sphingolipids,
ceramide, glycolipids
ISOF/BioFreeRa
dicals
Consiglio
Nazionale delle
Ricerche
++39-051639-8309
40129
Bologna
Via Piero Gobetti
101
Italy
trans lipid, radical
stress, isomerization
EPR Lipidomics
Dept Biochemistry ++39 080
and Molecular
5443367
Biology
70126
BARI
V. E. Orabona, 4
Italy
phospholipid bilayer,
spin labeling EPR,
lipoperoxidation,
oxidative stress
Istituto
Superiore di
Dept of Cell
Biology and
00161
ROME
viale Regina
Elena, 299
Italy
isoprostanes,
prostaglandins,
++39-0649903087
rapid, simple, serial analysis of intermolecular associations between
different cell surface proteins, (C) a simple method to completely block
Pgp function using an anti-Pgp mAb, (D) a xenotransplantation system
in SCID mice to study, by SPECT and PET, the effect of modulators, (E)
methods for fluorescence resonance energy transfer (FRET)
measurements on cell surface proteins to study inter-, and
intramolecular distances. We have flow-cytometers, confocal
microscope equipped with FCS, expertise in fluorescence-related
methods, with special emphasis on FRET.
Our interest is to understand the role of fatty acid metabolism :
synthesis and desaturation specifically, in pathologies leading to the
alteration of glucose and lipid homeostasis.The main emphasis is on
nutritional essential fatty acids. We have developed animal and cell
culture models of : hypercholesterolemia ,fatty liver and diabetes. We
are employing "in vitro" and "in vivo" labelled precursors to follow up lipid
synthesis, fatty acid desaturation, cholesterol , bile acid synthesis and
lipid composition.We measure the enzymatic pathways involved. We
utilize GC, HPLC and TLC methodologies , as well as biochemical and
molecular biology determinations. Our purpose is to contribute to the
understanding of the impact of nutritional fatty acids on the
amelioration of lipid related diseases .
For 15 years. my lab has worked on sphingolipids. We focus on
sphingolipid synthesis, and also defective degradation in lysosomal
storage diseases. More details can be found at:
http://www.weizmann.ac.il/Biological_Chemistry/scientist/futerman/
Different aspects of the cis-trans isomerization of fatty acid residues by
free radicals are developed: (a) Biomimetic studies using liposomes as
a model for cell membranes and correlating the isomerization process
with possible mechanisms of lipid alteration occurring in vivo. The role of
different vitamins. (b) A general approach has been settled for buildingup of trans lipid library with application to lipidomic researches.
Analytical data on trans lipids and characterization of these lipid
components in living organisms, thus contributing to the lipidomic target.
This is also related to the examination of biological samples and
correlation between trans lipid and radical stress. Endogenous formation
of trans lipids and correlation with quality of life and various pathologies.
Phospholipid bilayer structure and biochemical/biophysical properties.
Spin labeling of phospholipids. EPR studies of spin labelled natural and
artificial membranes (mitochondria, erythrocyte ghost, lipid vesicles,
oriented planar membranes). Lipoperoxidation. Synthesis and
purification of oxidized phospholipid species. EPR studies of the effects
of oxidized phospholipids in model membranes and in membranes
reconstituted with lipidomes from pathological tissues (animal models
and humans) and from cultured pathological cell mutants. Mass
spectrometry lipidomics of peroxidized phospholipids, mainly lecithins (in
collaboration with the local university Chemistry Department).
isoprostanes, prostaglandins, oxidative stress
36
scientific
scientific
scientific
scientific
scientific
Sanità
Anna Maria
Vaccaro Unit of
Physiopatology
of genetic
diseases
Neurosciences
Istituto Superiore
di Sanita
++39 06
4990 2416
00161
Roma
Viale Regina
Elena 299
Italy
Department of
Cell Biology and
Oncology
Mario Negri Sud
Institute
+39
0872570353
66030
Santa Maria
Imbaro
Via Nazionale 8/A
Italy
oxidative stress
sphingolipidoses,
saposins,
glucosylceramidase,
anionic phospholids+
phosphoinositides,
glycerophosphoinositol
s, phospholipases,
LXR-FXR nuclear
receptors
We works on a group of genetic diseases, the sphingolipidoses,
scientific
characterized by the lysosomal storage of sphingolipids due to the
defective activities of sphingolipid hydrolases or of their physiological
activators. In particular, we have investigated the alterations in
glucosylceramide catabolism in Niemann Pick disease type C. We have
shown that the storage of cholesterol in Niemann Pick type C modulates
the level and subcellular localization of glucosylceramidase affecting in
this way the glucosylceramide degradation (Salvioli et al., J.Biol. Chem.
279, 17674-17680, 2004). Moreover, we have shown that the
glucosylceramidase N370S mutation, the most common mutation in
Gaucher disease, affects the capacity of the enzyme to interact with
anionic phospholipid-containing membranes and saposin C, the main
physiological activators of glucosylceramidase (Salvioli et al., Biochem.
J., 390, 95-105, 2005). Our work is also focused on the study of the
mechanism/s of action of saposins and on their interaction with lipids,
especially phospholipids. Actually we have contribute to define the
mechanism of action of saposin C (Vaccaro et al.,J.Biol.Chem.,272,
16862-16867,1997, Salvioli et al., FEBS Letters, 472, 1721, 2000), of
saposin D (Ciaffoni et al., J. Biol. Chem., 276, 31583-31589, 2001) and
of saposin B (Ciaffoni et al., J. Lip. Res., 47, 1045-1053, 2006).
Phosphoinositide metabolism and related diseases. Our expertises
scientific
include: -- phosphoinositide analysis by standard HPLC, TLC, Lipid
binding domain recognition -- PI kinase and phosphatase assays -inositol phospate and glycerophosphoinositol analysis by HPLC and MS
-- metabolism of the phosphoinositides and their metabolites -Morphological approaches with reference to the LBD (IF, EM,
Tomography, FRET, FRAP, Correlative light/EM) Our aim is to set and
apply the lipidomic approach to the lipid profiling of cell models of
different pathological conditions (Overexpressing or KO for
proteins/enzymes involved in lipid metabolism and related diseases)
Ongoing projects: The glycerophosphoinositols (GPIs) as modulators of
the Rho-family cycle: Our studies have indicated that the GPIs, the
cellular derivatives of the phosphoinositides, are modulators of actin
cytoskeleton assembly through an action on the Rho GTPases (Corda et
al., 2002, Mancini et al., 2003). These compounds have thus become
the focus of further studies aimed at elucidating their mechanism of
action (Mariggiò et al, 2006) and at exploiting them as potential leads for
drug development in actin-related diseases (Wiscott-Aldrich syndrome,
X-linked mental retardation due to defect in PIX, common variable
immunodeficiency). Diseases due to defects in the PI phosphatases:
OCRL-1, MTM1 and MTMR2 (Lowe Syndrome, Myotubular Myopathy,
and Type 4B Charcot-Marie-Tooth). The aim of our project is to identify
and validate the enzymes controlling the PI cycle as pharmacological
targets, the activities of which can be modulated by inhibitors and/or
activators to re-establish the correct balance between the PIs pools in
the above-mentioned conditions in which this balance has been
compromised by the lack or deregulation of the PIPtases. A similar
approach is currently being undertaken in the case of the deregulation of
37
Department of
Cell Biology and
Oncology
Mario Negri Sud
Institute
+39
0872570353
66030
Santa Maria
Imbaro
Via Nazionale 8/A
Italy
phosphoinositides,
glycerophosphoinositol
s, phospholipases,
LXR-FXR nuclear
receptors
Biochemistry
group
Department of
Experimental
Medicine
Dipartimento di
Scienze
Biochimiche
University MilanoBicocca
University MilanoBicocca
++39-026448-8203
++39-026448-8203
20052
Monza
Via Cadore, 48
Italy
20052
Monza
Via Cadore, 48
Italy
Alzheimer, Lipid rafts,
signal transduction
Alzheimer, Lipid rafts,
signal transduction
PI 3-kinase (PI3K) pathway, which occurs in cancer, thrombotic
diseases, inflammation and diabetes. Other projects involving aspects of
the lipid metabolism are: -role of PLA2 in membrane traffic -mechanism
of membrane fissioning mediated by the protein BARS -Regulation of
lipid metabolism by LXR and FXR nuclear receptor and relevance in the
development of colon cancer
Phosphoinositide metabolism and related diseases. Our expertises
scientific
include: -- phosphoinositide analysis by standard HPLC, TLC, Lipid
binding domain recognition -- PI kinase and phosphatase assays -inositol phospate and glycerophosphoinositol analysis by HPLC and MS
-- metabolism of the phosphoinositides and their metabolites -Morphological approaches with reference to the LBD (IF, EM,
Tomography, FRET, FRAP, Correlative light/EM) Our aim is to set and
apply the lipidomic approach to the lipid profiling of cell models of
different pathological conditions (Overexpressing or KO for
proteins/enzymes involved in lipid metabolism and related diseases)
Ongoing projects: The glycerophosphoinositols (GPIs) as modulators of
the Rho-family cycle: Our studies have indicated that the GPIs, the
cellular derivatives of the phosphoinositides, are modulators of actin
cytoskeleton assembly through an action on the Rho GTPases (Corda et
al., 2002, Mancini et al., 2003). These compounds have thus become
the focus of further studies aimed at elucidating their mechanism of
action (Mariggiò et al, 2006) and at exploiting them as potential leads for
drug development in actin-related diseases (Wiscott-Aldrich syndrome,
X-linked mental retardation due to defect in PIX, common variable
immunodeficiency). Diseases due to defects in the PI phosphatases:
OCRL-1, MTM1 and MTMR2 (Lowe Syndrome, Myotubular Myopathy,
and Type 4B Charcot-Marie-Tooth). The aim of our project is to identify
and validate the enzymes controlling the PI cycle as pharmacological
targets, the activities of which can be modulated by inhibitors and/or
activators to re-establish the correct balance between the PIs pools in
the above-mentioned conditions in which this balance has been
compromised by the lack or deregulation of the PIPtases. A similar
approach is currently being undertaken in the case of the deregulation of
PI 3-kinase (PI3K) pathway, which occurs in cancer, thrombotic
diseases, inflammation and diabetes. Other projects involving aspects of
the lipid metabolism are: -role of PLA2 in membrane traffic -mechanism
of membrane fissioning mediated by the protein BARS -Regulation of
lipid metabolism by LXR and FXR nuclear receptor and relevance in the
development of colon cancer
Lipids and neurodegenerative diseases Lipid rafts and disease Lipid
scientific
rafts and signal transduction Lipid-protein interaction
Lipids and neurodegenerative diseases Lipid-protein interaction Lipid
scientific
rafts and signal transduction Lipid rafts and disease
University of
Florence
++39055459
8328
50143
Firenze
Viale G.B.
Morgagni 50
Italy
sphingosine 1phosphate, lipid
microdomain,
Sphingosine 1-phosphate and its mechanism of action. Regulation of
cellular sphingosine 1-phosphate content. Functional role of membrane
lipid microdomains in sphingosine 1-phosphate production and
38
scientific
Department of
Clinical and
Experimental
Medicine,
Physiopathology
, University
School of
Medicine
University of
Perugia
++39-0755729085
06100
Perugia
Policlinico
Monteluce, via
Brunamonti
Italy
ceramide, ceramidase signalling.
intranuclear lipid, DNA- We have demonstrated that a phospholipid cellular fraction is associated scientific
lipid, RNA-lipid
with chromatin. The presence, described by histochemical and
biochemical techniques, is not due to membrane contaminations. The
chromatin phospholipid fraction (CPF) can be considered a chromatin
minor component. The CPF differ from that of microsomes and nuclear
membranes as regard composition and turnover and changes in relation
to hepatocyte maturation and proliferation. The main modifications,
evident in several cellular functions, regard principally
phosphatidylcholine (PC) and sphingomyelin (SM) that are metabolised
directly in the nuclei in a very short time with a fine modulation of the
enzimatic activities, regulated by PLs. The presence of a complex of
base exchange enzymes for PC synthesis, a neutral sphingomyelinase
(N-SMase), a sphingomyelin-synthase (SM-synthase), a
phosphatidylcholine-dependent phospholipase C (PC-PLC) and a
sphingomyelin-synthase-reverse (SM-synthase-reverse), which
synthesises PC from SM have been demonstrated. These enzymes
differ for pH and Km optima from those present in nuclear membranes,
suggesting the possible existence of different isoforms. These results
strongly support the presence of a metabolic machinery in the chromatin
which probably is regulated in relation to cellular function. The PC-PLC
and SM-synthase enrich the intranuclear pool of diacylglicerol (DAG),
whereas the N-SMase and SM-synthase-reverse enrich the intranuclear
pool of ceramide. DAG and ceramide are know as second messengers
which play an important role in different physiological conditions, acting
as a stimulating (DAG) and inhibitory factor (ceramide) of protein kinase
C (PKC) The hypothesis has been made that the lipid messenger can
favour the nuclear molecular events which precede DNA synthesis in
liver regeneration. The results show that the DAG pool increases at the
beginning of S phase, whereas the ceramide pool later increases. In the
erythroleukemic cells, treated with DMSO and/or D3 vitamin, the NSMase activity increases during the apoptotic process, whereas PC-PLC
activity increases during cell differentiation. It will be interesting to know
if DAG and ceramide, produced directly in chromatin, can play a role in
cellular functions. 1. Viola Magni M.P., Gahan P.B., Albi E., Iapoce R.
and Gentilucci P.F. "Chromatin phospholipids and DNA synthesis in
hepatic cells". Bas. Appl. Histochem. 29, 253-259, 1985. I.F. 1.041 2.
.Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F.
"Phospholipids in chromatin: incorporation of 32PO24 in different
subcellular fraction of hepatocytes" Cell Biochem. and Function 4, 283288, 1986. I.F. 1.452 3. Viola Magni M.P., Gahan P.B., Albi E., Iapoce
R. and Gentilucci P.F. "Synthesis of chromatin phospholipids" Bas. Appl.
Histochem. 31, 355-364, 1987. I.F. 1.041 4. Albi E., Viola Magni M.P.
and Gahan P.B. "Age-related changes in chromatin phospholipid
fraction" The liver, metabolism and ageing, Eurage 13,189-188, 1989.
5. Gahan P.B., Albi E. and Viola Magni M.P. "Changes with age in
phospholipid composition of rat liver cell nuclei and nuclear envelopes".
Drug metabolism, liver injury and ageing, Eurage, 16, 229-236, 1991. 6.
Albi E., Viola Magni M.P., Lazzarini R. and Gahan P.B. "Chromatin
39
phospholipid changes during rat liver development" Cell Biochem. and
Function 9, 119-123, 1991. 1.452. I.F. 1.452 7. Albi E. "Presenza e ruolo
dei fosfolipidi nella cromatina" Tesi di Dottorato in Patologia Cellulare e
Molecolare. 1991. 8. Fraschini A., Albi E., Gahan P.B. and Viola Magni
M.P. "Tem cytochemical study of the localization of phospholipids in
interphase chromatin in rat hepatocytes" Histochemistry 97, 225-235,
1992. I.F 9. Albi E. , Mersel M., Leray C., Tomassoni M.L. and ViolaMagni M.P. Rat Liver Chromatin Phospholipids" Lipids 29, 715-7191994. I.F. 2.164 10. Albi E., Micheli M., Viola-Magni M.P. "Phospholipids
and nuclear RNA" Cell Biol. Intern. 20, 6, 1996. I.F. 1.092 11. Albi E.,
Viola-Magni M.P. "Choline base exchange activity in rat hepatocyte
nuclei and nuclear membrane" Cell Biol.Intern 21, 217-221, 1997. I.F.
1.092 12. Albi E., Tomassoni M.L., Viola-Magni M.P. "Effect of lipid
composition on rat liver nuclear membrane fluidity" Cell Biochem. and
Funct, 15, 181-190, 1997. I.F. 1.452 13. Albi, E. and Viola Magni, M.P.
"Chromatin neutral spingomyelinase and its role in hepatic regeneration.
Biochim. Biophys. Res. Commun., 236, 29-33, 1997. I.F. 2.836 14.
Micheli,M., Albi,E, Leray,C., and Viola Magni,M.P. "Nuclear
sphingomyelin protects RNA from RNase action" FEBS Letters 431,
443-447, 1998. I.F. 3.609 15. Tomassoni,M.L., Albi,E., and Viola
Magni,M.P. Changes of nuclear membrane fluidity during rat liver
regeneration Biochem. Mol. Biol. Intern. 47, 1049-1059, 1999. 16.
Albi,E., Peloso,I., and Viola Magni,M.P. Nuclear Membrane
Sphingomyelin-Cholesterol Changes in Rat Liver after Hepatectomy
Biochem. Biophys. Res. Commun. 262, 692-695, 1999. I.F. 2.836 17.
Albi,E., Viola Magni,M.P. Sphingomyelin-Synthase in Rat Liver Nuclear
Membrane and Chromatin FEBS Letters 460, 369-372, 1999. I.F. 3.609
18. Albi,E., and Viola Magni,M.P. Phosphatidylcholine-Dependent
Phospholipase C in Rat Liver Chromatin
Biochem.Biophys.Res.Commun. 265, 640-643, 1999. I.F. 2.836 19.
Albi,E., Viola Magni,M.P. The presence and the role of chromatin
cholesterol in rat liver regeneration Journal of Hepatology 36, 395-400,
2002. I.F. 5.285 20. Caso,V., Panarelli,P., Albi,E., Viola-Magni,M.P.,
Parnetti,L., Gallai,V. Phospholipid autoantibodies: time for a new
immuno-assay? Clin Exp Hypertens. 24: 511-516, 2002. I.F. 0.816 21.
Panarelli,P., Viola-Magni,M.P., and Albi E. Antiphosphatidylinositol
antibody in deep venous thrombosis patients Int.J. Immunopath. Pharm.
2003, 16: 61-6. I.F. 3.927 22. Albi,E., Cataldi,S., Rossi,G., and Viola
Magni,M.P. A possible role of cholesterolsphingomyelin/phosphatidylcholine in nuclear matrix during rat liver
regeneration. J. Hepatology 2003, 38: 623-8. I.F. 5.283 23. Albi,E.,
Pieroni,S., Viola Magni,M.P., and Sartori,C Chromatin sphingomyelin
changes in cell proliferation and/or apoptosis induced by ciprofibrate. J.
Cell Physiol. 2003, 196:354-61. I.F. 5.436 24. Valeria Caso, Lucilla
Parnetti, Paolo Panarelli, Maria Pia Viola Magni, MD, Virgilio Gallai,
Elisabetta Albi Selection of Thrombogenetic Antiphospholipid Antibodies
In Cerebrovascular Disease Patients J.Neurol 2003, 250: 593-597. I.F.
2.778 25. Albi,E., Rossi,G., Maraldi,N.M., Viola Magni,M.P., Cataldi,S.,
Solimando, L., Zini,N. Involvement of nuclear Phosphatidylinositol-
40
Department of
Clinical and
Experimental
Medicine,
Physiopathology
, University
School of
Medicine
University of
Perugia
++39-0755729085
06100
Perugia
Policlinico
Monteluce, via
Brunamonti
Italy
dependent Phospholipases C in cell cycle progression during rat liver J.
Cell Physiol. 2003, 197: 181-188. I.F. 5.463 26. Elisabetta Albi, Remo
Lazzarini and Mariapia Viola Magni Reverse Sphingomyelin-Synthase in
Rat Liver Chromatin FEBS Letters 2003, 549(1-3):152-156. I.F. 3.609
27. Elisabetta Albi, Samuela Cataldi, Mariapia Viola Magni and Claudia
Sartori Plasmalogens in rat liver chromatin: new molecules involved in
cell proliferation J. Cell Physiol, 2004, 201(3):439-46. I.F. 5.463 28.
Elisabetta Albi, Samuela Cataldi, Elisa Bartoccini, Mariapia Viola Magni,
Francesca Marini, Francesca Mazzoni, Giuseppe Rainaldi, Monica
Evangelisti, Mercedes Garcia-Gil Nuclear sphingomyelin pathway in
serum deprivation-induced apoptosis of embryonic hippocampal cells J
Cell Physiol, 2005. I.F. 5.463 29. Elisabetta Albi, Caterina AM La Porta ,
Samuela Cataldi, Mariapia Viola Magni Nuclear sphingomyelin-synthase
and protein kinase C delta in melanoma cells Arch. Biochem Biophys,
2005, 438:156-61. I.F. 2.338
intranuclear lipid, DNA- We have demonstrated that a phospholipid cellular fraction is associated scientific
lipid, RNA-lipid
with chromatin. The presence, described by histochemical and
biochemical techniques, is not due to membrane contaminations. The
chromatin phospholipid fraction (CPF) can be considered a chromatin
minor component. The CPF differ from that of microsomes and nuclear
membranes as regard composition and turnover and changes in relation
to hepatocyte maturation and proliferation. The main modifications,
evident in several cellular functions, regard principally
phosphatidylcholine (PC) and sphingomyelin (SM) that are metabolised
directly in the nuclei in a very short time with a fine modulation of the
enzimatic activities, regulated by PLs. The presence of a complex of
base exchange enzymes for PC synthesis, a neutral sphingomyelinase
(N-SMase), a sphingomyelin-synthase (SM-synthase), a
phosphatidylcholine-dependent phospholipase C (PC-PLC) and a
sphingomyelin-synthase-reverse (SM-synthase-reverse), which
synthesises PC from SM have been demonstrated. These enzymes
differ for pH and Km optima from those present in nuclear membranes,
suggesting the possible existence of different isoforms. These results
strongly support the presence of a metabolic machinery in the chromatin
which probably is regulated in relation to cellular function. The PC-PLC
and SM-synthase enrich the intranuclear pool of diacylglicerol (DAG),
whereas the N-SMase and SM-synthase-reverse enrich the intranuclear
pool of ceramide. DAG and ceramide are know as second messengers
which play an important role in different physiological conditions, acting
as a stimulating (DAG) and inhibitory factor (ceramide) of protein kinase
C (PKC) The hypothesis has been made that the lipid messenger can
favour the nuclear molecular events which precede DNA synthesis in
liver regeneration. The results show that the DAG pool increases at the
beginning of S phase, whereas the ceramide pool later increases. In the
erythroleukemic cells, treated with DMSO and/or D3 vitamin, the NSMase activity increases during the apoptotic process, whereas PC-PLC
activity increases during cell differentiation. It will be interesting to know
if DAG and ceramide, produced directly in chromatin, can play a role in
cellular functions. 1. Viola Magni M.P., Gahan P.B., Albi E., Iapoce R.
41
and Gentilucci P.F. "Chromatin phospholipids and DNA synthesis in
hepatic cells". Bas. Appl. Histochem. 29, 253-259, 1985. I.F. 1.041 2.
.Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F.
"Phospholipids in chromatin: incorporation of 32PO24 in different
subcellular fraction of hepatocytes" Cell Biochem. and Function 4, 283288, 1986. I.F. 1.452 3. Viola Magni M.P., Gahan P.B., Albi E., Iapoce
R. and Gentilucci P.F. "Synthesis of chromatin phospholipids" Bas. Appl.
Histochem. 31, 355-364, 1987. I.F. 1.041 4. Albi E., Viola Magni M.P.
and Gahan P.B. "Age-related changes in chromatin phospholipid
fraction" The liver, metabolism and ageing, Eurage 13,189-188, 1989.
5. Gahan P.B., Albi E. and Viola Magni M.P. "Changes with age in
phospholipid composition of rat liver cell nuclei and nuclear envelopes".
Drug metabolism, liver injury and ageing, Eurage, 16, 229-236, 1991. 6.
Albi E., Viola Magni M.P., Lazzarini R. and Gahan P.B. "Chromatin
phospholipid changes during rat liver development" Cell Biochem. and
Function 9, 119-123, 1991. 1.452. I.F. 1.452 7. Albi E. "Presenza e ruolo
dei fosfolipidi nella cromatina" Tesi di Dottorato in Patologia Cellulare e
Molecolare. 1991. 8. Fraschini A., Albi E., Gahan P.B. and Viola Magni
M.P. "Tem cytochemical study of the localization of phospholipids in
interphase chromatin in rat hepatocytes" Histochemistry 97, 225-235,
1992. I.F 9. Albi E. , Mersel M., Leray C., Tomassoni M.L. and ViolaMagni M.P. Rat Liver Chromatin Phospholipids" Lipids 29, 715-7191994. I.F. 2.164 10. Albi E., Micheli M., Viola-Magni M.P. "Phospholipids
and nuclear RNA" Cell Biol. Intern. 20, 6, 1996. I.F. 1.092 11. Albi E.,
Viola-Magni M.P. "Choline base exchange activity in rat hepatocyte
nuclei and nuclear membrane" Cell Biol.Intern 21, 217-221, 1997. I.F.
1.092 12. Albi E., Tomassoni M.L., Viola-Magni M.P. "Effect of lipid
composition on rat liver nuclear membrane fluidity" Cell Biochem. and
Funct, 15, 181-190, 1997. I.F. 1.452 13. Albi, E. and Viola Magni, M.P.
"Chromatin neutral spingomyelinase and its role in hepatic regeneration.
Biochim. Biophys. Res. Commun., 236, 29-33, 1997. I.F. 2.836 14.
Micheli,M., Albi,E, Leray,C., and Viola Magni,M.P. "Nuclear
sphingomyelin protects RNA from RNase action" FEBS Letters 431,
443-447, 1998. I.F. 3.609 15. Tomassoni,M.L., Albi,E., and Viola
Magni,M.P. Changes of nuclear membrane fluidity during rat liver
regeneration Biochem. Mol. Biol. Intern. 47, 1049-1059, 1999. 16.
Albi,E., Peloso,I., and Viola Magni,M.P. Nuclear Membrane
Sphingomyelin-Cholesterol Changes in Rat Liver after Hepatectomy
Biochem. Biophys. Res. Commun. 262, 692-695, 1999. I.F. 2.836 17.
Albi,E., Viola Magni,M.P. Sphingomyelin-Synthase in Rat Liver Nuclear
Membrane and Chromatin FEBS Letters 460, 369-372, 1999. I.F. 3.609
18. Albi,E., and Viola Magni,M.P. Phosphatidylcholine-Dependent
Phospholipase C in Rat Liver Chromatin
Biochem.Biophys.Res.Commun. 265, 640-643, 1999. I.F. 2.836 19.
Albi,E., Viola Magni,M.P. The presence and the role of chromatin
cholesterol in rat liver regeneration Journal of Hepatology 36, 395-400,
2002. I.F. 5.285 20. Caso,V., Panarelli,P., Albi,E., Viola-Magni,M.P.,
Parnetti,L., Gallai,V. Phospholipid autoantibodies: time for a new
immuno-assay? Clin Exp Hypertens. 24: 511-516, 2002. I.F. 0.816 21.
42
Department of
Internal
Medicine
University of
Perugia
0039 75
5857423
06122
Perugia
Via del Giochetto
Italy
pollen phospholipids,
brain mitochondria,
anionic phospholipids,
cardiolipin
Panarelli,P., Viola-Magni,M.P., and Albi E. Antiphosphatidylinositol
antibody in deep venous thrombosis patients Int.J. Immunopath. Pharm.
2003, 16: 61-6. I.F. 3.927 22. Albi,E., Cataldi,S., Rossi,G., and Viola
Magni,M.P. A possible role of cholesterolsphingomyelin/phosphatidylcholine in nuclear matrix during rat liver
regeneration. J. Hepatology 2003, 38: 623-8. I.F. 5.283 23. Albi,E.,
Pieroni,S., Viola Magni,M.P., and Sartori,C Chromatin sphingomyelin
changes in cell proliferation and/or apoptosis induced by ciprofibrate. J.
Cell Physiol. 2003, 196:354-61. I.F. 5.436 24. Valeria Caso, Lucilla
Parnetti, Paolo Panarelli, Maria Pia Viola Magni, MD, Virgilio Gallai,
Elisabetta Albi Selection of Thrombogenetic Antiphospholipid Antibodies
In Cerebrovascular Disease Patients J.Neurol 2003, 250: 593-597. I.F.
2.778 25. Albi,E., Rossi,G., Maraldi,N.M., Viola Magni,M.P., Cataldi,S.,
Solimando, L., Zini,N. Involvement of nuclear Phosphatidylinositoldependent Phospholipases C in cell cycle progression during rat liver J.
Cell Physiol. 2003, 197: 181-188. I.F. 5.463 26. Elisabetta Albi, Remo
Lazzarini and Mariapia Viola Magni Reverse Sphingomyelin-Synthase in
Rat Liver Chromatin FEBS Letters 2003, 549(1-3):152-156. I.F. 3.609
27. Elisabetta Albi, Samuela Cataldi, Mariapia Viola Magni and Claudia
Sartori Plasmalogens in rat liver chromatin: new molecules involved in
cell proliferation J. Cell Physiol, 2004, 201(3):439-46. I.F. 5.463 28.
Elisabetta Albi, Samuela Cataldi, Elisa Bartoccini, Mariapia Viola Magni,
Francesca Marini, Francesca Mazzoni, Giuseppe Rainaldi, Monica
Evangelisti, Mercedes Garcia-Gil Nuclear sphingomyelin pathway in
serum deprivation-induced apoptosis of embryonic hippocampal cells J
Cell Physiol, 2005. I.F. 5.463 29. Elisabetta Albi, Caterina AM La Porta ,
Samuela Cataldi, Mariapia Viola Magni Nuclear sphingomyelin-synthase
and protein kinase C delta in melanoma cells Arch. Biochem Biophys,
2005, 438:156-61. I.F. 2.338
I)Plant pollens are an important source of environmental antigens that
scientific
stimulate allergic responses. In addition to acting as vehicled for foreign
protein antigens, they contain different molecular species of lipids, which
are necessary in the reproduction of higher plants. The CD1 family of
nonpolymorphic major histocompatibility complex-related molecules is
highly conserved in mammals, and has been shown to present microbial
and self lipids to T cells. We provided evidence that pollen lipids may be
recognized as antigens by human T cell through a CD1-dependent
pathway. Amonng phospholipids extracted from cypress grains,
phosphatidylcholine (PC)and phosphatidylethanolamine (PE)were able
to stimulate the proliferation of T cells from cypresse-sensitive subjects.
Particularly, 18:2/18:2 PC, a predominat component of cypress PC,
could be one of the specific targets in pollen grain capture and
recognition by CD1. Also pollen-derived unsaturated PE are antigens
recognized by a variety of T clones derived from allergic subjects. The
nature of the acyl chains in the lipid antigens contributes enormously to
immunogenicity.(Agea et al. Journal Exp. Med. (2005) 202:295-308. The
nature of lipid-protein interaction between phospholipids and CD1 is a
target for future investigation. The antigenic properties of glycolipids
extracted and purified from pollens will be also evaluated. II) We are
43
Department of
Internal
Medicine,
Section of
Biochemistry
University of
Perugia
++39-075585-7420
I-06122
Perugia
Via del Giochetto
Italy
Department of
Internal
Medicine,
Section of
Biochemistry
University of
Perugia
++39-075585-7420
I-06122
Perugia
Via del Giochetto
Italy
also involved in projects aimed at studying the role of anionic
phospholipids, particularly cardiolipin, in the functionality of mitochondria
in the nervous tissue. A major finding of our previous studies was the
effect exerted by exogenous phospholipids on the release of cytochrome
c from the inner mitochondrial membrane and on the transmembrane
potential of brain mitochondria. Mitochondria were enriched with
exogenous phosoholipids through a fusion process in the outer
mitochondrial membrane. The experimental system utilized a fluorescent
probe inserted in the outer mitochondrial membrane to follow the
kinetics of incorporation of lipids in mitochondria. By using a selective
probe for cardiolipin, it has been demonstrated thet the fused lipid can
reach the inner mitochondrial membrane and influence the membrane
potential. We found that exogenous cardiolipin reinforced the binding of
cytochrome c with the inner mitochondria, thus hindering its release
following different stimuli. The interaction of cytochrome c with the
components of the inner mitochondrial membrane is object of
investigation. The mode of membrane association of cytochrome c is
studied by using a model system of cytochrome c reconstituted in
different cardiolipins. (Piccotti et al. JBC 2002, 277:12075-12081,
Piccotti et al. J. Membr. Biol. 2004, 198:43-53)
phospholipase A2, lipid This group is interested in the identification and localization of various
scientific
mediators, PAF
phospholipases A2 in intracellular compartments. Particular attention is
devoted to secretory PLA2s with the aim of uderstanding their functions
in normal and pathological conditions. We have already demonstrated
the presence of a group IIA sPLA2 from cerebral cortex mitochondria
and its release under energy-deficient conditions and the location of
group V sPLA2 in the nuclei of cultured PC-12 and U251 astrocytome
cells of group V sPLA2 (Macchioni et al., J Biol Chem. (2004) Vol. 279,
36 (3), 37860-37869). Furthermore we have set up a procedure for a
continuous monitoring PLA2 activities in cultured cells in vivo or
subcellular organelles. Since many cell types release low molecular
weight sPLA2, we are also studying the routing for their secretion of their
translocation to intracellular compartments. This group is also
interested on the formation of lipid mediators and particularly of PAF.
We have identified the presence of the enzymes for its synthesis in the
nervous tissue.
phospholipase A2, lipid This group is interested in the identification and localization of various
scientific
mediators, PAF
phospholipases A2 in intracellular compartments. Particular attention is
devoted to secretory PLA2s with the aim of uderstanding their functions
in normal and pathological conditions. We have already demonstrated
the presence of a group IIA sPLA2 from cerebral cortex mitochondria
and its release under energy-deficient conditions and the location of
group V sPLA2 in the nuclei of cultured PC-12 and U251 astrocytome
cells of group V sPLA2 (Macchioni et al., J Biol Chem. (2004) Vol. 279,
36 (3), 37860-37869). Furthermore we have set up a procedure for a
continuous monitoring PLA2 activities in cultured cells in vivo or
subcellular organelles. Since many cell types release low molecular
weight sPLA2, we are also studying the routing for their secretion of their
translocation to intracellular compartments. This group is also
44
Funato Group
Hiroshima
University
Osaka University
Graduate School
of Medicine
++81-82424-7925
++81-66879-3283
7398528
6620018
HigashiHiroshima
Suita
1-4-4,
Kagamiyama
2-2 Yamadaoka
Japan
Japan
ceramide, GPI,
transport
HPLC/MS,
sphingolipids,
phospholipids
Department of
Metabolome,
Graduate School
of Medicine
Laboratory
Genetic
Metabolic
Diseases
The University
ofTokyo
81-3-58413651
1130033
Tokyo
7-3-1, Hongo,
Bunkyo-ku
Japan
lipid, database
Academic Medical +31-20Center
5669111
NL1100
DD
Amsterdam
P.O. Box 22660
Netherlan
ds
tandem-Mass
Spectrometry,
glycerophospholipids,
cardiolipin, ceramides
DSM
analysis/spectrom
etry
Dept Internal
Medicine, UMC
Nijmegen
Leiden Institute of
Physics, Leiden
University
0031
152792278
-
2600
MA
6500
HB
Delft
p.o. box 1
Nijmegen
Geert Grooteplein
zuid 8
Netherlan
ds
Netherlan
ds
ceramides,
sphingolipids
GC-MS, steroids,
lipoproteins
+31 71 527
5982
2333
CA
Leiden
Niels Bohrweg 2
Netherlan
ds
NIOZ
NIOZ
31 222
369582
1790
AB
Den Burg
P.O. Box 59
Netherlan
ds
cell signaling, model
systems, lipid rafts,
single-molecule
biophysics,
fluorescence
microscopy
organic geochemistry,
biomarker
Philips
Research,
Philips
+31-402743794
5656
AE
Eindhoven
High Tech
Campus 4
Netherlan
ds
cholsterol, membrane
microdomains,
Department of
Biochemistry
and Molecular
Biology
Lipid laboratory
Physics of Life
Processes
interested on the formation of lipid mediators and particularly of PAF.
We have identified the presence of the enzymes for its synthesis in the
nervous tissue.
lipid trafficking
I has developed an HPLC/ESI/MS-based method capable of analyzing a
wider range of lipid classes from neutral lipids to phospholipids on a
single chromatographic run with three solvent gradients and postcolumn mixing of solvent that helps ESI of lipids eluted earlier with nonpolar solvents. We used this method to study on lipid anomaly of a
variety of gene-targeted mice tissues and subcellular organellae [e.g.,
Takagi, S., Tojo, H., Tomita, S., Sano, S., Itami, S., Hara, M., Inoue, S.,
Horie, K., Kondoh, G., Hosokawa, K., Gonzalez, F.J. and Takeda, J.
Alteration of the 4-sphingenine scaffolds of ceramides in keratinocytespecific Arnt deficient mice affects skin barrier function. J. Clin. Invest.
112, 1372-1382 (2003)]. We are now extending the method amenable to
analyzing more polar lipids that cantain important bioactive lipids. We
eventually would like to develop a very comprehensive lipid analyzing
system.
create lipid database "LipidBank"
scientific
scientific
scientific
HPLC-tandem mass spectrometric analysis of lipid compositions at
scientific
different levels of complexity, such as cell compartments/organelles,
cells, tissues in order to clarify/study biochemical processes in relation to
disease based disorders. Research is biomedical orientated in a
university hospital setting and findings are where possible translated into
diagnostic tests.
ceramides, sphingolipids
industrial
lipidperoxydation and antioxidants, lipid transfer, steroids, nutrients
scientific
cell signaling, model systems, lipid rafts, single-molecule biophysics,
fluorescence microscopy
scientific
As part of the ICOMM (http://icomm.mbl.edu/)work we are building up a
lipid database. If possible, we want to combine our data with yours.
Firstly, we are interested in a procedure to find data from the database.
What programs are developed, how do they work. If there is no such
program exists, one has to be created. We hope we can work with you
to get things working.
Bioactive lipids relevant to diseases
scientific
45
industrial
Molecular
Diagnostics
Bijvoet Center
Utrecht University
++31-302533498
+31-302536616
NL-584
CH
3584
CH
Utrecht
Padualaan 8
Utrecht
Padualaan 8
Netherlan
ds
Netherlan
ds
sphingolipids, oxidized
lipids, lipoproteins
membrane membraneprotein rafts
lipid cell biology - lipid
mass spectrometry model membrane
biochemistry
Institute of
Biomembranes
Utrecht University
Nutrition,
metabolism and
genomics group
Wageningen
University
++31-317485787
6703
HD
Wageningen
Bomenweg 2
Netherlan
ds
transcriptomics,
PPARs, fatty acids
Preclinical
sciences
GE Healthcare
+47 2318
5666
0401
Oslo
Nycoveien 2
Norway
Phospholipids, blood,
drug development
Inst. for Cancer
Research/Sandv
igs
group:Intracellul
ar transport
The Norwegian
Radium Hospital
47 22934294 0310
Oslo
Montebello
Norway
glycosphingolipids,
toxins, cholesterol,
rafts
Laboratory of
Transcriptional
Regulation
Centre for
Medical Biology
PAS
++48-422723639
93-232
Lodz
Lodowa 106
Poland
ABC transporters,
gene expression,
transcription factors
Department of
Animal
Physiology and
Biophysics
Institute of
Biophysics,
Faculty of
Medicine
Faculty of
Biology,
University of
Bucharest
University of
Ljubljana
00-40-21318 15 69
050095
Bucharest
Splaiul
Independentei,
91-95
Romania
Plane lipid bilayers,
Liposomes,
Pharmacology
-386 1
5437600
1000
Ljubljana
Lipiceva 2
Slovenia
vesicle shapes,
membrane trafficking,
amphitropic proteins
Lipids is membranes and as signalling entities
scientific
The Institute of Biomembranes is an interdisciplinary research institute
and graduate school at Utrecht University, accommodating 18 research
groups from the faculties of Science, Medicine, and Veterinary Medicine.
The famous lipid biochemist Laurens van Deenen was one of the
founding members of the institute in 1991, but unfortunately deceased in
1994. To remember him, since 2004 the Institute annually awards the
"van Deenen Medal" to a leading active scientist in biomembrane
research (http://ib.bio.uu.nl/). The present director chairs a specific
support action of the European Commission entitled "The European
Lipidomics Initiative" (www.lipidomics.net). A number of groups in the
institute work on questions related to the structure and function of lipids
in cells, blood (lipoproteins) and model membranes. Notably, a
dedicated effort in lipid mass spectrometry is ongoing in the Dept. of
Biochemistry and Cell Biology, Faculty of Veterinary Medicine
(http://www.vet.uu.nl/bc).
Our group is interested in regulation of gene expression by fatty acids.
We use transcriptomics (in house affymetrix platform) in combination
with knock-out mice models to investigate the overall impact as well as
the mechanism of fatty acid-dependent gene regulation in numerous
organs (small intestine, liver, heart). Our expertise is: 1) design, analysis
and interpretation of transcriptomics experiments, and 2) pathway
mapping of transcriptomics data with special emphasis on lipid
metabolism
Phospholipid analysis related to contrast agents for medical imaging.
Analyses of blood samples in order to describe
pharmacokinetics/toxicokinetics.
Studies of the role of different lipids on intracellular transport of protein
toxins. For recent publications, please see the home
pages:http://radium.no/sandvig/ We have been studying the role of
cholesterol in transport, and we have been investigating cells with
mutations in (glyco)sphingolipid synthesis. Studies on the roles of
glycospingolipids are in progress.
Lipid hormones and second messengers - their direct and indirect
effects on gene expression. Lipid-binding transcription factors. Lipid
transporters from the ABC superfamily - mechanism of action, regulation
of expression.
Our group is interested to test antidepressants (natural and synthetic)
and neuroleptics on plane lipid bilayers by electrical recordings and
liposomes by fluorescence polarisation.
scientific
Mechanical properties of lipid membranes. Theoretical and experimental
studies of phospholipid vesicle shape behavior. Shape induced
mechanisms for the lateral segregation of membrane components. Role
of vesicle shapes in intracellular membrane trafficking. Mechanism of
scientific
46
scientific
industrial
scientific
scientific
scientific
INSTITUTO DE
PARASITOLOGI
AY
BIOMEDICINA
LOPEZ-NEYRA,
Working Group:
BIOCHEMISTR
Y AND
MOLECULAR
PHARMACOLO
GY. Dr.
FRANCISCO
GAMARRO
IQAC/Research
Unit on
BioActive
Molecules
CONSEJO
SUPERIOR DE
INVESTIGACION
ES CIENTÍFICAS
0034958181
667
18100
ARMILLAGRANADA
Parque
Tecnológico de
Ciencias de la
Salud. Avda. del
Conocimiento s
Spain
Parasites, lipid
translocation,
Aminophospholipid
translocases, ABC
transporters.
CSIC
34-934006115
08034
Barcelona
Jordi Girona 18
Spain
combinatorial
chemistry, nuclear
magnetic ressonance,
drug discovery,
environmental
contaminants
Unidad de
Biofísica
CSIC and
Universidad del
País Vasco
++34-94601-2625
48940
Leioa
Sarriena s
Spain
membrane lipids, lipid
biophysics, membrane
domains, lipid-protein
interaction
National Center
of Biotechnology
Spanish
Research Council
++34-915854840
28049
Madrid
Spain
signaling, chemotaxis,
leukocyte, HIV
Institute of
Molecular
Biology and
Genetics
Spanish
Research Council
& University of
Valladolid School
of Medicine
Universidad
Complutense
+34-983423-062
E47003
Valladolid
Darwin, 3.
Campus
Cantoblanco
University
Calle Sanz y
Fores s
Spain
++34-913944994
28040
Madrid
Fac. Biologia,
Dept. Bioquimica
Spain
Phospholipase A2,
Arachidonic Acid,
Prostaglandins,
Lysophosphatidylcholin
e
pulmonary surfactant,
monolayers,
membrane domains,
surface activity
University of
Murcia
++34968364766
E30100
Murcia
Facultad de
Veterinaria,
Spain
BIOMIL
(BIOphysics of
Membranes and
Lipid/Protein
Interfaces)
Research Group
in
action of amphitropic proteins studied by vesicle shape transformations
induced by their binding to vesicle membranes.
Lipid translocation in arasites of health interest: their implications in the
biology of parasites, and their potential use as drug targets.
Aminophospholipid translocases and ABC transporters in parasites: its
role in drug resistance and infectivity of parasites.
Drug discovery and pharmacological tools: Effects of combinatorial
libraries of synthetic chemicals on cell sphingolipid profiles. Toxicity of
environmental contaminants: Deciphering whether the toxicity of
selected environmental contaminants occurs by altering the sphingolipid
composition of affected cells. Sphingolipid maps as sensors of
environmental contamination. Analytical methodology: (1) Development
of 15N and 31P NMR procedures to construct sphingolipid and
phospholipid maps. (2) Application of biocompatible chemical reactions
to the construction of pseudolipid maps and validation of this approach
to research in cell biology.
We are interested in the biophysics of membrane lipids, and lipid-protein
interactions, with an emphasis on sphingolipids, lipid signalling,
membrane domains, and, more recently, lipoproteins. Our techniques
include calorimetry (DSC, ITC), spectroscopy (IR, fluorescence, UV-vis,
stopped-flow, CD), confocal microscopy, Langmuir balance, and
supported membranes.
The previous work of my group has been focused in understand how
lipid rafts organize cell signaling during leukocyte chemotaxis as well as
in the pivotal function of these microdomains at the early steps of the
human immunodeficiency virus infection.
Eicosanoids, Lysophospholipids, Glycerophospholipids
Membrane lateral structure and its role in the establishment of lipidprotein and protein-protein interactions. Lipids and lipid/protein
complexes as surface-active agents, with particular emphasis in the
structure and molecular mechanisms of membranes and surface layers
of natural pulmonary surfactant and clinical preparations used in the
therapeutical treatment of respiratory diseases.
lipid-protein interaction, Protein-lipid interactions: interactions with model membranes of
PKCs, diacylglycerol,
signalling proteins, such as PKCs and Bcl-2 family of proteins
47
scientific
scientific
scientific
scientific
scientific
scientific
scientific
Biomembranes,
Department of
Biochemistry
and Molecular
Biology
Campus de
Espinardo
Dept.
Physiology,
Medical School
University of the
Basque Country
++34946012
846
48940
The WennerGren Institute
Stockholm
University
++46-8164127
Department of
Crop Science
Swedish
University of
Agricultural
Sciences
Umeå University
Department of
Biophysical
Chemistry
LC-MS Support
Life Sciences
Mass
Spectrometry
University of
Basel,
Experimental
Immunology
Division of
Biochemistry
Bilbao
liposomes
Sarriena
Spain
Cholesterol, Liver,
VLDL secretion,
functional genomics
SE1069 Stockholm
1
Arrhenius lab. F3
Sweden
please fill in
23053
Alnarp
Box 44
Sweden
fatty acid metabolism,
elongase, gene
expression
plant oil biosynthesis
+469078652
28
SE90187
Umeå
Linaeus väg 10
Sweden
domains, lateral
diffusion, Lipid/protein
Applera Europe
B.V.
+41 41 799
7742
6343
Rotkreuz
Grundstrasse 10
Switzerlan
d
phospholipid,
eiscosanoids, PAF,
biomarker
School of
Pharmaceutical
Sciences University of
Geneva
University
Hospital,
Department of
Research
University of
Fribourg
+41-223796344
1211
Geneva
Bd Yvoy 20
Switzerlan
d
Mass spectrometry,
Pharmaceuticals,
Proteins, C.elegans
+41 61
2652365
4031
Basel
Hebelstrasse, 20
Switzerlan
d
0041 26 300
8630
CH1700
Fribourg
5, chemin du
musée
Switzerlan
d
associated to the regulation of cell apoptosis. Lipid regulation of the
activity of PKCs. The action of a variety of bioactive lipìds on membrane
translocation and activation of the Protein Kinase C family of proteins
associated to cell signalling. The action of lipids such as
phosphatidylserine, phosphoinositides, free fatty acids, diacylglycerols
and ceramides is invetigated. Techniques used: NMR (CPMAS,
HRMAS, deuterium NMR, 31P-NMR), DSC, ITC, Biacore, Fluorescence
spectroscopy, confocal microscopy, X-ray diffraction.
Disorders of lipid and lipoprotein metabolism involved in hepatic and
cardiovascular diseases. Molecular mechanisms underlying
nonalcoholic fatty liver disease. Functional genomics in nonalcoholic
fatty liver disease.
Our topic is the regulation of very long chain fatty acid syntersis in
mammals and their significance in lipid metabolism.
scientific
scientific
please fill in
scientific
Lipid/protein interaction, regulation of lipid composition in cell
membranes, Formation of domains in lipid membranes, Lipid lateral
diffusion, Molecular ordering in membranes,.
Understanding of succession at developing and self stabilising cell
populations and response/adaptation in the membrane lipid composition
to stress conditions. Reveal cell signaling processes and interactions
with the environment depending on lipid molecules. Lipid synthesis and
degradation depend on enzyme activities and specific genes.
Supressing specific lipid synthesis process or facilitating adaptation
processes may identify genes involved and clarify function of so far
unknown gene sequences. Assessment of regulatory lipids
(eicosanoids, PAFs) as presymptomatic harbingers of pulmonary
pathobiology. Development of rapid and easy-to-use methods for
assessemnt and analysis of phospholipid profiles based on mass
spectrometry. Rapid and sensitive quantification of signaling lipid
molecules. Since 2007 LC-MS Support for Switzerland and South of
germany Area, providing expertise in Lipidanalysis by LC-MS
please fill in
scientific
immune recognition of
lipids
Identification of llipids with immunogenic activity and involved in
activating lipid-specific T cells in diseases
scientific
Ceramide, GPI, cell
wall, heat shock,
cholesterol, ergosterol,
Biosynthesis, Remodeling and transport and cell wall integration of GPI
anchored proteins of Saccharomaces cerevisiae. Sphingolipid
biosynthesis of S. cerevisiae. Sterol transport, neutral lipid storage and
scientific
48
industrial
scientific
Institute of
Cellular and
Organismic
Biology, Section
of Stem Cells
Institute of
Bioinformatics
and Structural
Biology
Dept. of Medical
Biochemistry &
Immunology,
School of
Medicine
Chemical
Engineering Life
Science
Interface
School of Life
Sciences
steryl esters, lipases,
lipid bodies
farnesyl
pyrophosphate,
geranylgeranyl
pyrophosphate, E-Ras
degradation. Lipid rafts transport and sortining of integral membrane
proteins.
Prenyl pyrophosphates as differentiation signals for stem cells
Academia Sinica
++886-227899531
11529
Taipei
Nankang
Taiwan
National Tsing
Hua University
886-35742752
30043
Hinchu
Kung-Fu Rd
Taiwan
glycosphingolipid,
cardiotoxin,
phospholipase A2
Our main interest is to understand the role of various lipids and/or lipid
domain responsible for the action of cobra venom components such as
cobra cardiotoxins or phospholipase A2.
scientific
Cardiff University
0044 29
2074 8447
CF14
4XN
Cardiff
Heath Park
United
Kingdom
please fill in
please fill in
scientific
Chemical and
Process
Engineering
University of
Sheffield
Heriot-Watt
University
+440780971735
5
S3 7RD Sheffield
40 LeavyGreave
Road
United
Kingdom
Sulfolipids,
sulfoglycolipids,
cyanobacteria,
bioactive compounds
My area of interest is to mining lipid profile of cyanobacteria for
identifying potential drug candidates.
scientific
++44 131
451 3186
EH14
4AS
Edinburgh
Roccarton
United
Kingdom
Diet-gene interaction,
lipogenesis,fungal lipid
metabolism
scientific
scientific
The Chemical
Biology Centre
in the
Department of
Chemistry
Imperial College
+44 (0)20
London in
7594 5787
association with
the Institute of
Cancer Research
and the London
Research Institute
of CRUK
SW7
2AZ
London
Exhibition Road
United
Kingdom
Lipid biophysical
characterisation
Nature Reviews
Molecular Cell
Biology
Glycobiology
NPG
+44(0)20784
33641
N1
9XW
London
4 Crinan St
United
Kingdom
lipids
We are interested in the influence of diet on the regulation of gene
expression in hepatic tissue culture, in particular emphasis on lipogenic
genes. A second area of interest focusses on fungal lipid metabolism,
particularly in the oleaginous Yarrowia lipolytica.
Biophysical properties of lipids Phase behaviour studied by SAXRD,
NMR, SSNMR, polarising microscopy, DSC, high pressure SAXRD
Mesophase structure and energetics measured by SAXRD osmometry,
SSNMR, ITC, DSC, direct
vesicle manipulation and modelling using
continuum elastic theories, mesoscopic modelling and full atomistic
modelling (QMMD) Dynamics of phase transformations (the lipids role
in cell division, fusion etc.) using
synchrotron X-ray scattering on
rapidly perturbed systems Studies of the short and long range
coupling of membrane charge and curvature elasticity Lipid-protein and
lipid-drug interactions Studies of the effect of membrane elasticity on
enzyme activity, extrinsic membrane protein binding, membrane
protein refolding dynamics, drug binding Studies of the coupling of
membrane chemistry and biochemistry to lipid elastic properties
Studies of lipid liquid ordered phases and protein associations within
these domains Studies of membrane protein assembly, distribution
and dynamics Development of novel single cell membrane proteomics
and lipidomics A recently funded project to handle single cells inoptical
traps within microfluidic flows,
strip off membrane portions, separate
components and analyse using a novel 2D-IR spectroscopy
lipids
Oxford University
+44 1865
OX1
Oxford
South Parks Rd
United
Oligosaccharide
Protein and lipid glycosylation and diseases, including the lysosomal
49
scientific
scientific
scientific
Institute
275725
3QU
Kingdom
analysis, lysosomal
storage
extraction analysis
Shell Global
Solutions (UK)
Refsum disease
group
Shell Global
Solutions (UK)
St Thomas
Hospital
+44-151373-5730
++44207188
1256
CH13S
H
SE1
7EH
Chester
P.O. Box 1
London
Lambeth Palace
Road
United
Kingdom
United
Kingdom
Mass
Spectrometry
Facility
The School of
Pharmacy
++44-2077535876
WC1N
1AX
London
29/39 Brunswick
Square
United
Kingdom
sterols, steroids, lipid
rafts
European
application
laboratory
WELLCOME
TRUST
BIOCENTRE,
SCHOOL OF
LIFE
Thermo Electron
+44
1442233555
HP2
7GE
Hemel
Hempstead
1 Boundary Park
United
Kingdom
UNIV OF
DUNDEE
++44(0)1382388688
DD1
5EH
DUNDEE
HAWKHILL
United
Kingdom
mass spectrometry,
phospholipids,
sphingolipids
PROTOZA LIPID
BIOSYNTHESIS
peroxisome, alphaoxidation, omegaoxidation, isoprenoid,
phytanic
storage disorders (Gaucher disease for example). Glycolipid
(sphingolipid) analysis by HPLC and mass spectrometry. Chemistry and
biology of imino sugars that modulate glycolipid biosynthesis
Interest in extraction and analysis techniques for lipids
industrial
The group has been interested in the transport and biochemical
scientific
pathways of phytanic acid metabolism and thus alpha- and omegaoxidation pathways of alpha-methyl isoprenoid fatty acids. This pathway
has recently been defined with the cloning of key enzymes in alphaoxidation including phytanoyl-CoA 2-hydroxylase (PAHX), 2hydroxyphytanoyl-CoA lyase, alpha-methylacyl-CoA racemase together
with confirmation of their localisation in peroxisomes. PAHX, an iron(II)
and 2-oxoglutarate dependent oxygenase is located on chromosome
10p13. Mutant forms of PAHX have been shown to be responsible for
some, but not all, cases of Refsums Disease. Certain cases have been
shown to be atypical mild variants of rhizomelic chondrodysplasia
punctata type 1a. Other atypical cases with low plasma phytanic acid
may be caused by a-methylacyl-CoA racemase deficiency. A sterolcarrier protein 2 (SCP-2) knockout mouse model shares a similar clinical
phenotype to Refsums Disease, but no mutations in SCP-2 have been
described to date in man. SCP-2 acts as a solubilistaion
factor/intracellular carrier for these hydrophobic fatty acids. Work on
the omega-oxidation pathway for these fatty acids has clarified the role
of cytochrome 4A1 enzymes in the intitial hydroxylation to dicarboxylic
acids and the subsequent peroxisomal beta-oxidation pathway. It has
also led to interest in this pathway as a therapeutic option for
peroxiosomal diseases. The pathway for synthesis of phytanic acid
from phytol in man and possibly other mammals has also been clarified
with the identification of the enzymes in the pathway and identification of
FALDH-10 - the enzyme deficient in Sjogren-Larsson syndroem as one
of the key enzymes in this pathway. The group is also interested in the
role of PhyH outside the preroxisome where it may act as protein
regulator and the possible importance of phytanic acid as a PPAR-alpha
ligand.
The research in the mass spectrometry group at the School of
scientific
Pharmacy is focused on lipids and their interactions with proteins. Major
efforts are being made in the development of new methodology for the
mass spectrometric analysis of steroids and sterols. In parrallel with
lipidomic studies, we are investigating the protein content of lipid rafts
and studying protein-lipid interactions.
methods in mass spectrometry for high throughput lipid analysis
industrial
MY GROUP WORKS ON PHOSPHO- AND GLYCO- LIPID
BIOSYNTHESIS IN PROTOZOAN PARASITES SUCH AS T.BRUCEI,
THE CAUSATIVE AGENT OF AFRICAN SLEEPING SICKNESS. WE
USE TOOLS SUCH AS FOWARD AND REVESRE GENETICS TO
VALIDATE GENES AS DRUG TARGETS PRIOR TO EXPLORING
50
scientific
SCIENCES,
UNIV OF
DUNDEE
Cardiovascular
Genetics,
University College +44 207 679
London
6968
WC1E
6JF
London
5 University St
United
Kingdom
Aberdeen
lipidomix group
University of
Aberdeen
44 (0)1224
553020
AB25
2ZD
Aberdeen
Polwarth building,
Foresterhill
United
Kingdom
Department of
Pharmacy &
Pharmacology
Institute for
Cancer Studies
University of Bath
++-44-12253867686
BA2
7AY
Bath
Claverton Down
United
Kingdom
University of
Birmingham
44-(0)121414-3293
B15
2TT
Birmingham
Vincent Drive
United
Kingdom
School of
Pharmacy
University of
Bradford
++44-1274224717
BD7
1DP
Bradford
Richmond Road
United
Kingdom
Department of
Pharmacology
University of
Cambridge
44-1223334032
CB2
1PD
Cambridge
Tennis Court
Road
United
Kingdom
Dept. of
Biochemistry
School of
Chemistry
University of
Oxford
University of
Southampton
+44 1865
275371
++44
(0)2380
796161
OX1
3QU
SO17
1BJ
Oxford
Southampto
n
South Parks Road United
Kingdom
University Road
United
Kingdom
Centre for
Equine and
Animal Science
Writtle College
44 1245
424200
CM1
3RR
Chelmsford
Lordship Road
United
Kingdom
Centre for
Equine and
Animal Science
Writtle College
44 1245
424200
CM1
3RR
Chelmsford
Lordship Road
United
Kingdom
SUBSTRATE AND INHIBITOR SELECTIVITY. WE HAVE START TO
SET UP A PARASITE LIPIIDOME DATABASE FOR THE COMMUNITY
TO USE AND SUBMIT PROFILES TO.
genes, apolipoproteins, Cardiovascular Genetics
triglyceridie
metabolism
inflammationfunction of biologically active lipids in cardiovascular disorders. Effect of
atherosclerosisdietary components on the expression and secretion of biologically
endothelial fonctionactive lipid derivatives in relation to inflammatory disorders.
diet
Enzyme, phytanic acid, Enzaymes involved in lipid metabolism, branched-chain lipids and their
isoprenoids, long-chain role in diseases, long-chain fatty alcohols,
fatty alcohols
phosphoinositide,
1. Analysis of lipid signalling in particular phospholipases D and C and
mass spectrometry,
PI-3-kinase. 2. Use of MS methods to analyse lipids in mammalian,
phospholipase, cancer dictyostelium, yeast and drosophila cells in order to determine functions
of signalling pathways. 3. Analysis of lipids in tumour cells isolated by
techniques such as laser capture microdissection. 4. Analysis of
changes in lipids in other diseases for example vasculitis. 5.
Development of MS methods to quantify all phosphoinositides.
eicosanoids, brain,
eicosanoids and other lipid mediators molecular mechanism of action of
skin, mass
omega-3 fatty acids with emphasis on eicosapentaenoic acid
spectrometry
eicosanoid-mediated pathways and signalling systems mass
spectrometry high field NMR computational lipidomics cardiovascular
disease neurodegenerative diseases brain function cancer melanocytes
multidrug transporters, We study the molecular bases of the interactions of ATP-binding
steroids,
cassette transporters of human (e.g. ABCG1 and ABCG2) and bacterial
structure/function
origin (e.g., MsbA and LmrA) with chemotherapeutic drug and steroids
and other lipids. We also study the potential physiological roles of
multidrug transporters in lipid transport.
membrane protein
membrane protein/lipid interactions biomolecular simulations
phospholipids, mass
spectrometry, dynamic
lipidomics
scientific
scientific
scientific
scientific
scientific
scientific
scientific
Mass spectrometry of lipids Dynamics of phospholipid synthesis
scientific
Modelling lipid synthetic networks Lipid biomarkers in health and
disease Synthesis, composition and function of endonuclear lipids Lung
surfactant in health and disease Interaction between genotypic
expression and diet in the regulation of the molecular species
composition of cell lipids in vivo and in vitro Phospholipase-mediated cell
signalling Membrane fusion Lipids in stem cell differentiation Lipids and
nutrition Lipids in inflammation Oxidised phospholipids and
cardiovascular disease
Nutritional and biochemical role of fatty acids in the health and
scientific
performance of farm and companion animals.
poyunsaturated fatty
acids, conjugated
linoeic acids, immunity,
health
poyunsaturated fatty
Nutritional and biochemical role of fatty acids in the health and
acids, conjugated
performance of farm and companion animals.
linoeic acids, immunity,
scientific
51
University of
Iowa
Dept. of Molecular 1-319-335Physiology and
7874
Biophysics
52246
Iowa City
6-530 Bowen
Science Building
United
States
health
multidrug resistance,
sphingolipids,
mitochondria
Indiana
Umiversity Purdue
University
Indianapolis
Institute of
Molecular
Medicine and
Genetics
Indiana
Umiversity Purdue University
Indianapolis
317-2740593
46202
Indianapolis
723 W. Michigan
St.
United
States
yeast, sterol,
ergosterol
Medical College
of Georgia
01-706-7210699
GA
30912
Augusta
1120 15th Street
United
States
COBRE in
Lipidomics &
Pathobiology
COBRE in
Lipidomics &
Pathobiology
Center for
Developmental
Genetics
The Polt Group
Medical University 843-792of South Carolina 4323
29425
Charleston
PO Box 250509
United
States
Medical University 843-792of South Carolina 4323
29425
Charleston
PO Box 250509
United
States
Stony Brook
University
please fill in
11794
Stony Brook
438 CMM
United
States
The University of
Arizona
++01-520621-6322
AZ
85721
Tucson
Department of
Chemistry
San Diego
Supercomputer
Center / LIPID
MAPS
Bioinformatics
Core
University of
California San
Diego
858-8223619
92093
La Jolla
Nutrition and
Genomics
USDA-Human
Nutrition
Research Center
on Aging at Tufts
University
++1-617556-3102
02111
Boston
We study the interaction of sphingolipids and phospholipids with
multidrug transporters in the yeast Saccharomyces cerevisiae.
Transcription factors that modulate the expression of membrane
transporters, often in the plasma membrane, have recently been found
to also control the expression of genes involved in sphingolipid
biosynthesis. Our goal is to understand the physiological rationale this
coordinate control.
yeast sterol biosynthesis
scientific
Sphiogolipids,
glycolipids,
developmental biology,
neurodegenerative
diseases,
neurochemistry
cell growth, cell death,
cell aging,
inflammation
cell growth, cell death,
cell aging,
inflammation
please fill in
Analysis of glycolipid structure and metabolism in the nervous system,
lipid signaling molecules and signal trnaduction, cell-cell recognition and
adhesion, cell migration.
scientific
Define the function of these fatty molecules in human disease,
especially cancer, aging, neurologic disease and fungal pathogenesis.
scientific
Define the function of these fatty molecules in human disease,
especially cancer, aging, neurologic disease and fungal pathogenesis.
scientific
please fill in
scientific
United
States
PDMP,
glycosphingolipid,
sphingosine
9500 Gilman
Drive, Mail Code
0412
United
States
lipid classification, lipid
database, lipid
proteome, lipid
pathways
711 Washington
St
United
States
genetic
polymorphisms, genediet interactions,
perilipins,
Glycosphingolipids. Synthesis and structure elucidation. We are
scientific
involved with the design and synthesis of glycosidase and
glycosyltransferase inhibitors. We use Manduca sexta (tobacco horn
worm) as a model system to explore the effects of GSLs on
development.
http://www.chem.arizona.edu/faculty/profile/profile.php?fid_call=polt
The LIPID Metabolites and Pathways Strategy (LIPID MAPS)
scientific
Consortium represents a multi-institutional effort to develop a detailed
understanding of lipid structure and function. As part of this effort, we will
develop ‘parts lists’ of lipid metabolites and assemble these into
metabolic networks. These networks will then provide an infrastructure
for subsequent modeling using quantitative data from LIPID MAPS
experiments.
The Nutrition and Genomics Laboratory has been pionnering the study
scientific
of gene-diet interactions in the area of cardiovascular diseases, utilizing
both genetic epidemiology approaches as well as controlled dietary
intervention studies. This research involves the investigation of nutrientgene interactions in large and diverse populations around the world with
long-standing collaborations with investigators in Europe, Asia, Australia
and the United States. More recently our interest has been focusing on
52
scientific
Department of
Biochemistry
Wake Forest
University School
of Medicine
please fill in
27157
WinstonSalem
Medicial Center
Boulevard
United
States
phospholipase D,
bicelles, eicosanoids,
lipoproteins, lipid
kinases, virus
assesmbly
the genetic factors involved in fat metabolism in the adypocite and more
specifically the PATS family of genes. In addition, we seek to identify
genes involved in longevity and healthy aging and to understand their
regulation in response to dietary factors. For this research we use model
organisms such as Drosophila and mouse. Further comprehension of
the relation between genetic factors, nutrients and the rate of aging will
provide better understanding of the pathology of age-related diseases
and lead to improved strategies for their prevention.
The Department of Biochemistry (and affiliated faculty) at Wake Forest
University has a working group of faculty members (about 14 faculty
members) interested in the role of lipids in signal transduction,
atherosclerosis, cancer, host defense, and inflammation. Techniques in
use include thin-layer chromatography, HPLC, and mass spectrometry
to identify phospholipid and fatty acid species, vesicle and solid-phase
lipid binding assays, NMR spectroscopy and other biophysical
approaches, coupled with site-directed mutagenesis, to study structural
features of lipid:protein interactions, and computational modeling of
signaling pathways involving lipids. Professor McPhail represents the
lipid signaling working group on the departmental Development
Committee, which advises the Chair on departmental development and
policy.
53
scientific
Appendix 2: Lipidomics expertise survey results ordered by country and Institute (Expertise level ≥ 2; 0 = weak, 3 = strong;
LEP as per 01.01.2008)
Austria
Institute
Areas of Lipidomics Expertise
IMB Biochemistry - Yeast Genetics
Group
University of Graz
(Austria)
Institute for Genomics and
Bioinformatics
Graz University of Technology
(Austria)
Analytical Tools in Lipidomics,
Fatty Acids and Conjugates [FA01],
Genomics of the Lipidome, Lipid
Metabolism, Lipids in Microbiology,
Proteomics of the Lipidome,
Bioinformatics related to the Lipidome,
Genomics of the Lipidome, Lipid
Metabolism,
Institute for Molecular Biotechnology
Graz University of Technology
(Austria)
Genomics of the Lipidome, Lipid
Metabolism, Lipid Transport,
Membrane Microdomains, Membrane
Trafficking,
Institute of Biochemistry, Cell Biology
Group
Graz University of Technology
(Austria)
Analytical Tools in Lipidomics,
Glycerophosphocholines [GP01],
Genomics of the Lipidome, Lipid
Glycerophosphoethanolamines [GP02],
Metabolism, Lipid Transport, Lipids in Glycerophosphoinositols [GP06],
Microbiology, Membrane
Glycerophosphoserines [GP03], Sterols
Microdomains, Membrane Trafficking, [ST01],
Metabolomics of the Lipidome,
Proteomics of the Lipidome,
Genomics of the Lipidome, Lipid
Metabolism, Lipid Transport, Lipids and
Nutrition, Metabolomics of the
Lipidome, Pharmacology and
toxicology of the Lipidome, Signalomics
of the Lipidome,
Molecular Biology and Biochemistry,
Medical University Graz
(Austria)
Lipid Class - Survey
Sterols [ST01],
Technology - Survey
Organisms and cellular Systems Survey
Yeast,
Confocal Microscopy, Enzymatic
Assays, Fluorescence Microscopy, GC,
Gel Electrophoresis, Light Microscopy,
Thin layer chromatography,
Bioinformatics, Biostatistics, Cluster
Human Cell Lines, Mice, Mouse,
Analysis, Data Processing, Database
Setup, Fluorescence Microscopy, Light
Microscopy, Microarrays, Real Time
PCR,
DNA Sequencing, Enzymatic Assays, Eubacteria, Yeast,
Fluorescence Microscopy, GC, GC-MS
coupling, Gel Electrophoresis, Immuno
Assays, Light Microscopy, Real Time
PCR, Thin layer chromatography, UVVis Spectroscopy,
Enzymatic Assays, Fluorescence
Yeast,
Microscopy, GC, Gel Electrophoresis,
Immuno Assays, MALDI, Thin layer
chromatography, Two dimensional
Electrophoresis,
Belgium
Institute
Div. Pharmacology - Dep. Mol. Cell
Biology
K.U.Leuven
(Belgium)
Areas of Lipidomics Expertise
Organisms and cellular Systems Survey
Ceramides [SP02], Diradylglycerols
Chemical Synthesis, Enzymatic Assays, Eubacteria, Human Cell Lines, Human
[GL02], Fatty Acids and Conjugates
GC, Gel Electrophoresis, Ion Exchange, Primary, Mice, Mouse, Rats,
[FA01], Fatty aldehydes [FA06],
LC, Liquid-Liquid Extraction, Normal
Fattyamides [FA08], Isoprenoids
Phase, Reversed Phase, Size
[PR01], Neutral glycosphingolipids
Exclusion, Solid Phase Extraction, Thin
[SP05], Polyprenols [PR03], Sphingoid layer chromatography, UV-Vis
bases [SP01], Triradylglycerols [GL03], Spectroscopy,
Lipid Class - Survey
Technology - Survey
54
Laboratory for Experimental Medicine
and Endocrinology (LEGENDO)
Katholieke Universiteit Leuven
(Belgium)
Analytical Tools in Lipidomics,
Genomics of the Lipidome, Lipid
Metabolism, Mapping of the Lipidome,
Membrane Microdomains,
Metabolomics of the Lipidome,
Signalomics of the Lipidome,
DNA Sequencing, Enzymatic Assays, Biopsy, Human Cell Lines, Mice,
ESI, Fluorescence Microscopy, Gel
Electrophoresis, Immuno Assays, Light
Microscopy, Microarrays, nano ESI,
Real Time PCR, Thin layer
chromatography,
Denmark
Institute
Organisms and cellular Systems Survey
Analytical Tools in Lipidomics,
Ceramides [SP02], Diradylglycerols
APCI, Chemical Synthesis, CI, Cluster Biopsy, Blood, Mice, Rats, Stool,
Chemical Synthesis of Lipids
[GL02], Docosanoids [FA04],
Analysis, EI, Enzymatic Assays, ESI,
(Probes/Tools/Agents), Lipid
Eicosanoids [FA03], Fatty Acids and
GC, GC-MS coupling, Immune Affinity,
Metabolism, Lipid Signalling, Lipid
Conjugates [FA01], Fatty esters [FA07], Ion Exchange, LC, LC-MS coupling,
Standard Bank, Lipid Transport, Lipids Glycerophosphocholines [GP01],
Liquid-Liquid Extraction, Near Field,
and Nutrition, Mapping of the Lipidome, Glycerophosphoethanolamines [GP02], Normal Phase, Quadrupole, Reversed
Metabolomics of the Lipidome,
Glycerophosphoglycerophosphoglycerols Phase, Size Exclusion, Solid Phase
Nutrigenomics of the Lipidome,
(Cardiolipins) [GP12],
Extraction, Thin layer chromatography,
Glycerophosphoinositols [GP06],
Two dimensional Electrophoresis, UVGlycerophosphoserines [GP03],
Vis Spectroscopy,
Monoradylglycerols [GL01],
Octadecanoids [FA02],
Phosphosphingolipids [SP03], Sphingoid
bases [SP01], Sterols [ST01],
Triradylglycerols [GL03],
Areas of Lipidomics Expertise
Lipid Class - Survey
Technology - Survey
Institute
Areas of Lipidomics Expertise
Lipid Class - Survey
Technology - Survey
Institute of Biomedicine/Käkelä Group
University of Helsinki
(Finland)
Institute of Biomedicine/Somerharju
group
University of Helsinki
(Finland)
Mapping of the Lipidome, Signalomics
of the Lipidome,
Biochemistry and Nutrition Group,
Biocentrum-DTU
The Technical University of Denmark
(Denmark)
Finland
Analytical Tools in Lipidomics,
Bioinformatics related to the Lipidome,
Chemical Synthesis of Lipids
(Probes/Tools/Agents), Lipid
Metabolism, Lipid Standard Bank, Lipid
Transport, Mapping of the Lipidome,
Membrane Microdomains, Membrane
Trafficking, Metabolomics of the
Lipidome, Proteomics of the Lipidome,
Organisms and cellular Systems Survey
Acidic glycosphingolipids [SP06],
Chemical Synthesis, Cluster Analysis, Human Cell Lines,
Ceramides [SP02], Diradylglycerols
Confocal Microscopy, Data Processing,
[GL02], Fatty Acids and Conjugates
Database Setup, Enzymatic Assays,
[FA01], Glycerophosphates [GP10],
ESI, Fluorescence Microscopy,
Glycerophosphocholines [GP01],
Fluorescence Phosphorescence
Glycerophosphoethanolamines [GP02], Spectroscopy, Ion Trap, LC, LC-MS
Glycerophosphoglycerols [GP04],
coupling, Light Microscopy, LiquidGlycerophosphoglycerophosphates
Liquid Extraction, Normal Phase,
[GP05],
Quadrupole, Reversed Phase, Solid
Glycerophosphoglycerophosphoglycerols Phase Extraction, Thin layer
(Cardiolipins) [GP12],
chromatography, Triple Quadrupole,
Glycerophosphoinositol bisphosphates
[GP08], Glycerophosphoinositol
55
monophosphates [GP07],
Glycerophosphoinositols [GP06],
Glycerophosphoserines [GP03],
Monoradylglycerols [GL01], Neutral
glycosphingolipids [SP05],
Phosphosphingolipids [SP03], Sterols
[ST01], Triradylglycerols [GL03],
Quantitative Biology and Bioinformatics Analytical Tools in Lipidomics,
Ceramides [SP02], Diradylglycerols
APCI, Biostatistics, Cluster Analysis,
Biopsy, Blood, Human Cell Lines,
VTT Technical Research Centre of
Bioinformatics related to the Lipidome, [GL02], Docosanoids [FA04],
Data Processing, Database Setup, ESI, medicinal plants, Mice, Rats, Urine,
Finland
Glycomics of the Lipidome, Lipid
Eicosanoids [FA03], Fatty Acids and
GC, GC-MS coupling, Ion Exchange, Yeast,
(Finland)
Metabolism, Lipid Standard Bank,
Conjugates [FA01],
Ion Trap, LC, LC-MS coupling,
Lipids and Nutrition, Lipids in
Glycerophosphocholines [GP01],
Microarrays, nano ESI, Normal Phase,
Inflammation, Mapping of the Lipidome, Glycerophosphoethanolamines [GP02], Nuclear Magnetic Resonance, QqTOF,
Metabolomics of the Lipidome,
Glycerophosphoglycerophosphoglycerols Quadrupole, Real Time PCR, Reversed
Nutrigenomics of the Lipidome,
(Cardiolipins) [GP12],
Phase, Size Exclusion, Thin layer
Pharmacology and toxicology of the
Glycerophosphonocholines [GP16],
chromatography, TOF, Triple
Lipidome,
Glycerophosphonoethanolamines
Quadrupole,
[GP17], Glycerophosphoserines [GP03],
Isoprenoids [PR01], Monoradylglycerols
[GL01], Neutral glycosphingolipids
[SP05], Octadecanoids [FA02], Oxidized
glycerophospholipids [GP20],
Phosphosphingolipids [SP03], Sphingoid
bases [SP01], Sterols [ST01],
Triradylglycerols [GL03],
France
Organisms and cellular Systems Survey
Biological Chemistry Laboratory
Analytical Tools in Lipidomics, Lipid
Fatty Acids and Conjugates [FA01], Fatty Enzymatic Assays, ESI, GC, GC-MS
A thaliana, Agricultural Crop,
Biological Chemistry
Metabolism, Lipids in Microbiology,
ethers [FA10], Steroids [ST02], Sterols coupling, Gel Electrophoresis, Immuno Arabidopsis, Eubacteria, S cerevisae, Y
LaboratoryNational Institute for
Proteomics of the Lipidome,
[ST01], Triradylglycerols [GL03],
Assays, LC, LC-MS coupling, Reversed lipolytica, Yeast,
Agronomic Research (INRA)
Phase, Thin layer chromatography,
(France)
Two dimensional Electrophoresis, UVVis Spectroscopy,
IMBL-Lipid Signaling (UMR 585
Analytical Tools in Lipidomics, Lipid
Docosanoids [FA04], Eicosanoids
Brewster Angle Microscopy (BAM), Cell Blood, Human Cell Lines, Human
INSERM / INSA-Lyon)
Metabolism, Lipid Signalling, Lipids and [FA03], Fatty Acids and Conjugates
Sorting, Confocal Microscopy, EI,
Primary, Urine,
INSA-Lyon and CNRS / INSERM / Univ Nutrition, Membrane Microdomains,
[FA01], Fatty aldehydes [FA06],
Enzymatic Assays, Fluorescence
Lyon1
Metabolomics of the Lipidome,
Glycerophosphocholines [GP01],
Microscopy, GC, GC-MS coupling,
(France)
Nutrigenomics of the Lipidome,
Glycerophosphoethanolamines [GP02], Immuno Assays, Ion Trap, LC, LiquidSignalomics of the Lipidome,
Oxidized glycerophospholipids [GP20], Liquid Extraction, NCI, Normal Phase,
Quadrupole, Reversed Phase, Solid
Phase Extraction, Thin layer
chromatography,
INSERM U467/IFR94 Proteomics
Analytical Tools in Lipidomics, Lipid
Diradylglycerols [GL02], Eicosanoids
Cell Sorting, Confocal Microscopy,
Biopsy, Blood, Human Cell Lines, Mice,
Platform
Metabolism, Lipid Signalling, Lipids and [FA03], Fatty Acids and Conjugates
DNA Sequencing, Enzymatic Assays, Mouse, Urine,
Faculté de Médecine René Descartes Nutrition, Lipids in Inflammation,
[FA01], Glycerophosphocholines [GP01], ESI, Fluorescence Microscopy, GC,
Institute
Areas of Lipidomics Expertise
Lipid Class - Survey
Technology - Survey
56
Paris 5
(France)
Mapping of the Lipidome, Membrane
Microdomains, Membrane Trafficking,
Proteomics of the Lipidome,
Glycerophosphoethanolamines [GP02], GC-MS coupling, Gel Electrophoresis,
Glycerophosphoglycerols [GP04],
Immuno Assays, Ion Trap, LC, LC-MS
Glycerophosphoglycerophosphates
coupling, Light Microscopy, Liquid[GP05], Glycerophosphoinositols [GP06], Liquid Extraction, MALDI, Multicolor
Glycerophosphoserines [GP03],
Analysis, nano ESI, Normal Phase,
Monoradylglycerols [GL01], Sterols
Real Time PCR, Reversed Phase,
[ST01], Triradylglycerols [GL03],
Solid Phase Extraction, Thin layer
chromatography, TOF, Two
dimensional Electrophoresis,
INSERM U586 (Obesity Research Unit) Analytical Tools in Lipidomics, Lipid
Fatty Acids and Conjugates [FA01],
Electron Microscopy, Enzymatic
Biopsy, Blood, Human Cell Lines,
INSERM,
Metabolism, Lipid Signalling, Lipids and Other [GL00], Other [GP00],
Assays, Fluorescence Microscopy,
Human Primary, Mice, Mouse, Rats,
(France)
Nutrition, Lipids in Inflammation,
Triradylglycerols [GL03],
Liquid-Liquid Extraction, Real Time
Metabolomics of the Lipidome,
PCR, Thin layer chromatography, UVSignalomics of the Lipidome,
Vis Spectroscopy,
INSERM U671
Analytical Tools in Lipidomics,
Fatty Acids and Conjugates [FA01], Fatty Cell Sorting, Confocal Microscopy,
Biopsy, Human Cell Lines, Human
INSERM
Genomics of the Lipidome, Lipid
esters [FA07], Sterols [ST01],
DNA Sequencing, Enzymatic Assays, Primary, humans, Mice, Mouse, Rats,
(France)
Metabolism, Lipid Transport, Lipids and Triradylglycerols [GL03],
Fluorescence Microscopy, Gel
Nutrition, Membrane Trafficking,
Electrophoresis, Immuno Assays, Light
Metabolomics of the Lipidome,
Microscopy, Real Time PCR, Thin layer
Nutrigenomics of the Lipidome,
chromatography,
Proteomics of the Lipidome,
Signalomics of the Lipidome,
Institute of molecular and cellular
Analytical Tools in Lipidomics, Lipid
Confocal Microscopy, DNA
Human Cell Lines, Mice, Mouse,
pharmacology
Signalling, Lipids in Inflammation,
Sequencing, Fluorescence Microscopy,
CNRS UMR6097
Light Microscopy, MALDI, Microarrays,
(France)
Real Time PCR,
Laboratoire de Biogenèse Membranaire Analytical Tools in Lipidomics,
Diradylglycerols [GL02], Fatty Acids and Confocal Microscopy, Enzymatic
Agricultural Crop, Arabidopsis, Mice,
CNRS UMR 5200 Université V.
Genomics of the Lipidome, Lipid
Conjugates [FA01], Glycerophosphates Assays, GC, GC-MS coupling, Gel
Yeast,
Segalen Bordeaux 2
Metabolism, Lipid Signalling, Mapping [GP10], Glycerophosphocholines [GP01], Electrophoresis, Immune Affinity, Ion
(France)
of the Lipidome, Membrane
Glycerophosphoethanolamines [GP02], Exchange, LC, Microarrays, Normal
Microdomains, Membrane Trafficking, Glycerophosphoglycerols [GP04],
Phase, Real Time PCR, Size
Metabolomics of the Lipidome,
Glycerophosphoglycerophosphates
Exclusion, Thin layer chromatography,
Proteomics of the Lipidome,
[GP05],
Glycerophosphoglycerophosphoglycerols
(Cardiolipins) [GP12],
Glycerophosphoinositol bisphosphates
[GP08], Glycerophosphoinositol
monophosphates [GP07],
Glycerophosphoinositol trisphosphates
[GP09], Glycerophosphoinositols [GP06],
Glycerophosphonocholines [GP16],
Glycerophosphonoethanolamines
[GP17], Glycerophosphoserines [GP03],
Glyceropyrophosphates [GP11],
Hydrocarbons [FA11],
Monoradylglycerols [GL01],
Triradylglycerols [GL03],
57
Germany
Institute
Areas of Lipidomics Expertise
Albrecht-von-Haller-Institute for Plant
Sciences/Plant Biochemistry
Georg-August-University
(Germany)
Analytical Tools in Lipidomics,
Genomics of the Lipidome, Lipid
Metabolism, Lipid Signalling, Lipid
Standard Bank, Lipid Transport, Lipids
in Inflammation, Lipids in Microbiology,
Signalomics of the Lipidome,
department of cellular and molecular
pathology
DKFZ(German Cancer Research
Center)
(Germany)
Lipid Class - Survey
Diradylglycerols [GL02], Eicosanoids
[FA03], Fatty Acids and Conjugates
[FA01], Fatty aldehydes [FA06], Fatty
esters [FA07], Glycerophosphoinositol
bisphosphates [GP08],
Glycerophosphoinositol
monophosphates [GP07],
Glycerophosphoinositol trisphosphates
[GP09], Glycerophosphoinositols
[GP06], Monoradylglycerols [GL01],
Octadecanoids [FA02], Oxidized
glycerophospholipids [GP20],
Triradylglycerols [GL03],
Analytical Tools in Lipidomics,
Acidic glycosphingolipids [SP06], Basic
Bioinformatics related to the Lipidome, glycosphingolipids [SP07], Ceramides
Genomics of the Lipidome, Lipids in
[SP02], Fatty Acids and Conjugates
Inflammation, Membrane
[FA01], Neutral glycosphingolipids
Microdomains, Membrane Trafficking, [SP05],
Technology - Survey
APCI, CI, EI, Enzymatic Assays, ESI,
GC, GC-MS coupling, Gel
Electrophoresis, Ion Exchange, Ion
Trap, LC, LC-MS coupling, Liquid-Liquid
Extraction, NCI, Normal Phase,
Quadrupole, Reversed Phase, Size
Exclusion, Solid Phase Extraction, Thin
layer chromatography, TOF, Two
dimensional Electrophoresis,
Organisms and cellular Systems Survey
Agricultural Crop, Algae, Arabidopsis,
Yeast,
Biostatistics, Cell Sorting, Cluster
Biopsy, Mice, Mouse, Rats,
Analysis, Confocal Microscopy, Data
Processing, Electron Microscopy,
Fluorescence Microscopy, Gel
Electrophoresis, Immuno Assays, Light
Microscopy, Microarrays, nano ESI,
Real Time PCR, Thin layer
chromatography, Two dimensional
Electrophoresis,
Department of Physiological Chemistry Analytical Tools in Lipidomics,
Fatty Acids and Conjugates [FA01],
Biostatistics, Confocal Microscopy, Data Biopsy, Blood, Human Cell Lines,
University of Veterinary Medicine
Glycomics of the Lipidome, Membrane Fatty esters [FA07],
Processing, DNA Sequencing,
Human Primary,
Hannover
Microdomains, Membrane Trafficking, Glycerophosphocholines [GP01],
Enzymatic Assays, ESI, Fluorescence
(Germany)
Proteomics of the Lipidome,
Glycerophosphoethanolamines [GP02], Microscopy, GC, Gel Electrophoresis,
Glycerophosphoglycerols [GP04],
Immune Affinity, Ion Exchange, LC-MS
Glycerophosphoinositols [GP06],
coupling, Light Microscopy, Normal
Glycerophosphoserines [GP03], Neutral Phase, Real Time PCR, Reversed
glycosphingolipids [SP05], Other
Phase, Thin layer chromatography, Two
[FA00], Phosphosphingolipids [SP03], dimensional Electrophoresis,
Steroids [ST02], Sterols [ST01],
Dept. of Neonatology, Lipid Metabolism Analytical Tools in Lipidomics, Lipid
Fatty Acids and Conjugates [FA01],
Biostatistics, Cell Sorting, Data
bird lungs, Blood, Human Primary,
and Developmental Physiology Group Metabolism, Lipid Transport,
Glycerophosphocholines [GP01], Other Processing, dHPLC, ESI, Fluorescence induced sputa, lung lavage fluid, Rats,
Eberhard Karls Universität Tübingen
Metabolomics of the Lipidome,
[FA00], Phosphosphingolipids [SP03], Phosphorescence Spectroscopy, LC,
(Germany)
LC-MS coupling, Light Microscopy,
Liquid-Liquid Extraction, Normal Phase,
Quadrupole, Reversed Phase, Size
Exclusion, Solid Phase Extraction, Thin
layer chromatography, Triple
Quadrupole, UV-Vis Spectroscopy,
Institut for Food Chemistry
Analytical Tools in Lipidomics, Chemical Ceramides [SP02], Neutral
APCI, Chemical Synthesis, CI, EI,
Human Cell Lines, Human Primary,
University of Muenster
Synthesis of Lipids
glycosphingolipids [SP05], Other
Enzymatic Assays, ESI, Fluorescence
(Germany)
(Probes/Tools/Agents), Lipid
[SP00], Phosphosphingolipids [SP03], Phosphorescence Spectroscopy, GC,
58
Metabolism, Lipid Signalling, Lipids and Sphingoid bases [SP01],
Nutrition,
Institute for Clinical Chemistry
University of Regensburg
(Germany)
Institute of Medical Physics and
Biophysics
Medical Faculty, University of Leipzig
(Germany)
LIPIDOMIX GmbH
LIPIDOMIX GmbH
(Germany)
GC-MS coupling, Immune Affinity,
Infrared Spectroscopy, Ion Exchange,
Ion Trap, LC, LC-MS coupling, LiquidLiquid Extraction, Normal Phase,
Nuclear Magnetic Resonance,
Quadrupole, Reversed Phase, Size
Exclusion, Solid Phase Extraction, Thin
layer chromatography, Triple
Quadrupole, UV-Vis Spectroscopy,
Analytical Tools in Lipidomics,
Bile acids and derivatives [ST04],
Capillary Electrophoresis, Capillary
Biopsy, Blood, Human Cell Lines,
Bioinformatics related to the Lipidome, Ceramides [SP02], Eicosanoids [FA03], Electrophoresis, Cell Sorting, Confocal Human Primary, Mice, Stool, Urine,
Genomics of the Lipidome, Lipid
Fatty Acids and Conjugates [FA01],
Microscopy, Data Processing, Database
Metabolism, Lipid Transport, Lipids and Glycerophosphocholines [GP01],
Setup, dHPLC, DNA Sequencing, EI,
Nutrition, Lipids in Inflammation, Lipids Glycerophosphoethanolamines [GP02], Enzymatic Assays, ESI, Flow FRET,
in Skin Care, Mapping of the Lipidome, Glycerophosphoglycerols [GP04],
Fluorescence Microscopy, GC, GC-MS
Membrane Microdomains, Membrane Glycerophosphoglycerophosphates
coupling, Gel Electrophoresis, High
Trafficking,
[GP05], Glycerophosphoserines
Troughput Microscopy, Immuno
[GP03], Neutral glycosphingolipids
Assays, LC, LC-MS coupling, Light
[SP05], Phosphosphingolipids [SP03], Microscopy, Liquid-Liquid Extraction,
Sphingoid bases [SP01], Steroids
Microarrays, Multicolor Analysis,
[ST02], Sterols [ST01],
Nuclear Magnetic Resonance, Real
Time PCR, Size Exclusion, SNP
Analysis, Thin layer chromatography,
Triple Quadrupole, Two dimensional
Electrophoresis,
Analytical Tools in Lipidomics,
Docosanoids [FA04], Fatty aldehydes Atomic Force, Electron Spin
Biopsy, Blood,
Glycomics of the Lipidome, Lipids and [FA06], Glycerophosphates [GP10],
Resonance, Fluorescence Microscopy,
Nutrition, Lipids in Inflammation,
Glycerophosphocholines [GP01],
Infrared Spectroscopy, MALDI, Normal
Mapping of the Lipidome, Membrane
Glycerophosphoethanolamines [GP02], Phase, Nuclear Magnetic Resonance,
Microdomains,
Glycerophosphoglycerols [GP04],
Thin layer chromatography, TOF, UVGlycerophosphoglycerophosphates
Vis Spectroscopy,
[GP05], Glycerophosphoinositol
bisphosphates [GP08],
Glycerophosphoinositol
monophosphates [GP07],
Glycerophosphoinositol trisphosphates
[GP09], Glycerophosphoinositols
[GP06], Glycerophosphoserines
[GP03], Other [GL00], Oxidized
glycerophospholipids [GP20],
Phosphosphingolipids [SP03],
Triradylglycerols [GL03],
Analytical Tools in Lipidomics, Lipid
Docosanoids [FA04], Eicosanoids
Database Setup, ESI, LC, LC-MS
Biopsy, Blood, Human Primary, Mice,
Metabolism, Lipids and Nutrition, Lipids [FA03], Fatty Acids and Conjugates
coupling, Quadrupole, Reversed Phase, Rabbits, Rats, Urine,
in Inflammation, Mapping of the
[FA01], Fattyamides [FA08],
Solid Phase Extraction, Triple
Lipidome, Metabolomics of the
Glycerophosphocholines [GP01],
Quadrupole,
Lipidome, Nutrigenomics of the
Glycerophosphoethanolamines [GP02],
Lipidome, Pharmacology and toxicology Glycerophosphoglycerols [GP04],
59
of the Lipidome,
Glycerophosphoglycerophosphates
[GP05], Glycerophosphoinositol
bisphosphates [GP08],
Glycerophosphoinositol
monophosphates [GP07],
Glycerophosphoinositol trisphosphates
[GP09], Glycerophosphoinositols
[GP06], Glycerophosphoserines
[GP03], Octadecanoids [FA02],
Institute
Areas of Lipidomics Expertise
Lipid Class - Survey
Technology - Survey
Dept. Biophysics and Cell Biology
University of Debrecen
(Hungary)
Membrane Microdomains, Membrane
Trafficking,
Laboratory of Molecular Stress Biology
Institute of Biochemistry, Biological
Research Centre, HUng. Acad. Sci.
(Hungary)
Analytical Tools in Lipidomics,
Genomics of the Lipidome, Lipid
Metabolism, Lipid Signalling, Lipids in
Inflammation, Mapping of the Lipidome,
Membrane Microdomains,
Metabolomics of the Lipidome,
Pharmacology and toxicology of the
Lipidome, Proteomics of the Lipidome,
Signalomics of the Lipidome,
Analytical Tools in Lipidomics,
Genomics of the Lipidome, Lipid
Metabolism, Lipid Signalling, Lipid
Standard Bank, Lipids and Nutrition,
Lipids in Inflammation, Lipids in Skin
Care, Metabolomics of the Lipidome,
Nutrigenomics of the Lipidome,
Hungary
Laboratory of Nutritional Bioactivation
and Bioanalysis
Department of Biochemistry and
Molecular Biology, University of
Debrecen
(Hungary)
Organisms and cellular Systems Survey
Atomic Force, Cell Sorting, Confocal
Biopsy, Blood, Human Cell Lines,
Microscopy, Electron Microscopy, FLIM, Mouse, Urine, Yeast,
Flow FRET, Fluorescence Microscopy,
Fluorescence Phosphorescence
Spectroscopy, Gel Electrophoresis,
High Troughput Microscopy, Light
Microscopy, Multicolor Analysis, Real
Time PCR,
Italy
Institute
Areas of Lipidomics Expertise
Lipid Class - Survey
Technology - Survey
Department of Cell Biology and
Oncology
Mario Negri Sud Institute
(Italy)
Analytical Tools in Lipidomics, Lipid
Metabolism, Lipid Signalling, Lipid
Transport, Mapping of the Lipidome,
Membrane Microdomains, Membrane
Trafficking, Pharmacology and
Acidic glycosphingolipids [SP06], Bile
acids and derivatives [ST04],
Glycerophosphocholines [GP01],
Glycerophosphoinositol bisphosphates
[GP08], Glycerophosphoinositol
Cell Sorting, Confocal Microscopy,
Electron Microscopy, Enzymatic
Assays, ESI, Fluorescence Microscopy,
GC, Gel Electrophoresis, Immune
Affinity, Immuno Assays, Ion Exchange,
Organisms and cellular Systems Survey
Human Cell Lines, Human Primary,
Mice, Mouse, Rabbits, Rats, Yeast,
60
Department of Clinical and
Experimental Medicine,
Physiopathology, University School of
Medicine
University of Perugia
(Italy)
Department of Internal Medicine,
Section of Biochemistry
University of Perugia
(Italy)
ISOF/BioFreeRadicals
Consiglio Nazionale delle Ricerche
(Italy)
toxicology of the Lipidome, Proteomics monophosphates [GP07],
LC, LC-MS coupling, Light Microscopy,
of the Lipidome, Signalomics of the
Glycerophosphoinositol trisphosphates Liquid-Liquid Extraction, MALDI,
Lipidome,
[GP09], Glycerophosphoinositolglycans Multicolor Analysis, Normal Phase,
[GP15], Glycerophosphoinositols [GP06], Real Time PCR, Reversed Phase, Size
Isoprenoids [PR01], Neutral
Exclusion, Solid Phase Extraction, Thin
glycosphingolipids [SP05], Sterols
layer chromatography, Two
[ST01],
dimensional Electrophoresis,
Bioinformatics related to the Lipidome,
Blood, Human Cell Lines, Mouse, Rats,
Lipid Metabolism, Lipid Signalling, Lipid
Standard Bank, Membrane
Microdomains, Metabolomics of the
Lipidome, Pharmacology and
toxicology of the Lipidome, Signalomics
of the Lipidome,
Analytical Tools in Lipidomics, Lipid
Ceramides [SP02], Diradylglycerols
Confocal Microscopy, Enzymatic
Blood, Human Cell Lines, Rats,
Metabolism, Lipid Signalling, Lipid
[GL02], Eicosanoids [FA03], Fatty Acids Assays, Fluorescence Microscopy, GC,
Transport, Lipids in Inflammation,
and Conjugates [FA01], Fatty aldehydes Gel Electrophoresis, Immuno Assays,
Mapping of the Lipidome, Membrane [FA06], Fatty esters [FA07],
LC, Light Microscopy, Liquid-Liquid
Microdomains, Membrane Trafficking, Glycerophosphates [GP10],
Extraction, Multicolor Analysis, Normal
Metabolomics of the Lipidome,
Glycerophosphocholines [GP01],
Phase, Reversed Phase, Solid Phase
Pharmacology and toxicology of the
Glycerophosphoethanolamines [GP02], Extraction, Thin layer chromatography,
Lipidome, Signalomics of the Lipidome, Glycerophosphoglycerols [GP04],
Glycerophosphoglycerophosphates
[GP05],
Glycerophosphoglycerophosphoglycerols
(Cardiolipins) [GP12],
Glycerophosphoinositols [GP06],
Glycerophosphoserines [GP03],
Analytical Tools in Lipidomics,
Ceramides [SP02], Docosanoids [FA04], Chemical Synthesis, EI, Electron Spin Blood, erythrocyte membranes, Human
Chemical Synthesis of Lipids
Eicosanoids [FA03], Fatty Acids and
Resonance, Enzymatic Assays, ESI,
Cell Lines, Tetrahymena,
(Probes/Tools/Agents), Lipid
Conjugates [FA01], Fatty esters [FA07], Fluorescence Microscopy, GC, GC-MS
Metabolism, Lipid Standard Bank,
Glycerophosphocholines [GP01],
coupling, Infrared Spectroscopy, LC,
Lipids and Nutrition, Lipids in
Octadecanoids [FA02],
LC-MS coupling, Nuclear Magnetic
Inflammation, Mapping of the Lipidome, Phosphosphingolipids [SP03],
Resonance, Raman Spectroscopy,
Nutrigenomics of the Lipidome,
Triradylglycerols [GL03],
Reversed Phase, Solid Phase
Extraction, Thin layer chromatography,
UV-Vis Spectroscopy,
Netherlands
Institute
Areas of Lipidomics Expertise
Bijvoet Center
Utrecht University
(Netherlands)
Analytical Tools in Lipidomics,
Chemical Synthesis of Lipids
(Probes/Tools/Agents), Glycomics of
the Lipidome, Lipid Metabolism, Lipid
Signalling, Lipid Transport, Membrane
Microdomains, Membrane Trafficking,
Lipid Class - Survey
Technology - Survey
Organisms and cellular Systems Survey
61
Metabolomics of the Lipidome,
Proteomics of the Lipidome,
Signalomics of the Lipidome,
Institute of Biomembranes
Analytical Tools in Lipidomics,
Utrecht University
Glycomics of the Lipidome, Lipid
(Netherlands)
Metabolism, Lipid Signalling, Lipid
Transport, Mapping of the Lipidome,
Membrane Microdomains, Membrane
Trafficking,
Laboratory Genetic Metabolic Diseases Analytical Tools in Lipidomics, Lipid
Academic Medical Center
Metabolism, Membrane Trafficking,
(Netherlands)
Metabolomics of the Lipidome,
Nutrition, metabolism and genomics
group
Wageningen University
(Netherlands)
Acidic glycosphingolipids [SP06], Basic Cell Sorting, LC, Multicolor Analysis,
Arabidopsis, Blood, Eubacteria, Human
glycosphingolipids [SP07],
Nuclear Magnetic Resonance, Real
Cell Lines, Mice, Yeast,
Glycerophosphocholines [GP01],
Time PCR, Reversed Phase, Thin layer
Glycerophosphoethanolamines [GP02], chromatography,
Glycerophosphoserines [GP03], Neutral
glycosphingolipids [SP05],
Bile acids and derivatives [ST04],
CI, DNA Sequencing, EI, Enzymatic
Biopsy, Blood, Human Cell Lines, Mice,
Ceramides [SP02], Fatty Acids and
Assays, ESI, Fluorescence Microscopy, Urine, Yeast,
Conjugates [FA01],
GC, GC-MS coupling, Immune Affinity,
Glycerophosphocholines [GP01],
Immuno Assays, Ion Exchange, LC,
Glycerophosphoethanolamines [GP02], LC-MS coupling, Light Microscopy,
Glycerophosphoglycerols [GP04],
Liquid-Liquid Extraction, nano ESI, NCI,
Glycerophosphoglycerophosphates
Normal Phase, Quadrupole, Real Time
[GP05],
PCR, Reversed Phase, Size Exclusion,
Glycerophosphoglycerophosphoglycerols Solid Phase Extraction, Thin layer
(Cardiolipins) [GP12],
chromatography, Triple Quadrupole,
Glycerophosphoinositols [GP06],
UV-Vis Spectroscopy,
Glycerophosphoserines [GP03],
Isoprenoids [PR01],
Analytical Tools in Lipidomics,
Biostatistics, Cluster Analysis, Data
Bioinformatics related to the Lipidome,
Processing, Database Setup,
Genomics of the Lipidome, Lipid
Microarrays, Real Time PCR,
Metabolism, Lipid Signalling, Lipids and
Nutrition, Lipids in Inflammation,
Nutrigenomics of the Lipidome,
Institute
Areas of Lipidomics Expertise
Lipid Class - Survey
Technology - Survey
Institute of Biophysics, Faculty of
Medicine
University of Ljubljana
(Slovenia)
Membrane Microdomains, Membrane
Trafficking,
Glycerophosphocholines [GP01],
Fluorescence Microscopy, Light
Glycerophosphoserines [GP03],
Microscopy,
Phosphosphingolipids [SP03], Steroids
[ST02],
Organisms and cellular Systems Survey
Human Cell Lines,
Spain
Organisms and cellular Systems Survey
Institute of Molecular Biology and
Lipid Metabolism, Lipid Signalling,
Diradylglycerols [GL02], Eicosanoids
Capillary Electrophoresis, Cell Sorting, Blood, human, Human Cell Lines,
Genetics
Lipids in Inflammation, Mapping of the [FA03], Fatty Acids and Conjugates
Confocal Microscopy, DNA
Human Primary, Mice, Mouse, Rabbits,
Spanish Research Council & University Lipidome, Pharmacology and
[FA01], Glycerophosphates [GP10],
Sequencing, Enzymatic Assays,
Rats,
of Valladolid School of Medicine
toxicology of the Lipidome, Signalomics Glycerophosphocholines [GP01],
Fluorescence Microscopy, GC, Gel
(Spain)
of the Lipidome,
Glycerophosphoethanolamines [GP02], Electrophoresis, Immune Affinity,
Glycerophosphoglycerols [GP04],
Immuno Assays, Ion Exchange, LC,
Glycerophosphoglycerophosphates
Light Microscopy, Liquid-Liquid
[GP05],
Extraction, Multicolor Analysis, Normal
Institute
Areas of Lipidomics Expertise
Lipid Class - Survey
Technology - Survey
62
Glycerophosphoglycerophosphoglycerols Phase, Real Time PCR, Reversed
(Cardiolipins) [GP12],
Phase, Size Exclusion, Solid Phase
Glycerophosphoinositol bisphosphates Extraction, Thin layer chromatography,
[GP08], Glycerophosphoinositol
Two dimensional Electrophoresis,
monophosphates [GP07],
Glycerophosphoinositol trisphosphates
[GP09], Glycerophosphoinositols [GP06],
Glycerophosphoserines [GP03],
Glyceropyrophosphates [GP11],
Monoradylglycerols [GL01],
Triradylglycerols [GL03],
Switzerland
Institute
Areas of Lipidomics Expertise
Lipid Class - Survey
Technology - Survey
Division of Biochemistry
University of Fribourg
(Switzerland)
Analytical Tools in Lipidomics,
Genomics of the Lipidome, Lipid
Metabolism, Lipid Signalling, Lipid
Transport, Lipids in Microbiology,
Mapping of the Lipidome, Membrane
Microdomains, Membrane Trafficking,
Proteomics of the Lipidome,
Ceramides [SP02], Fatty Acids and
Confocal Microscopy, ESI,
Conjugates [FA01],
Fluorescence Microscopy, GC, Gel
Glycerophosphoglucose lipids [GP14], Electrophoresis, Light Microscopy, Thin
Glycerophosphoglycerophosphoglycerols layer chromatography,
(Cardiolipins) [GP12],
Glycerophosphoinositolglycans [GP15],
Glycerophosphoinositols [GP06],
Phosphosphingolipids [SP03], Sphingoid
bases [SP01], Steroid conjugates [ST05],
Steroids [ST02], Sterols [ST01],
Institute
Areas of Lipidomics Expertise
Lipid Class - Survey
European application laboratory
Thermo Electron
(United Kingdom)
Analytical Tools in Lipidomics,
Glycobiology Institute
Oxford University
(United Kingdom)
Analytical Tools in Lipidomics,
Bioinformatics related to the Lipidome,
Chemical Synthesis of Lipids
(Probes/Tools/Agents), Glycomics of
the Lipidome, Lipid Metabolism, Lipid
Signalling, Lipid Transport, Lipids in
Inflammation, Lipids in Microbiology,
Organisms and cellular Systems Survey
Yeast, yeast,
United Kingdom
Technology - Survey
Organisms and cellular Systems Survey
APCI, APPI, Biostatistics, CI, Cluster
Analysis, Data Processing, Database
Setup, EI, Enzymatic Assays, ESI,
FTICR, GC-MS coupling, Gel
Electrophoresis, Infrared Spectroscopy,
Ion Trap, LC, LC-MS coupling, MALDI,
nano ESI, NCI, Orbitrap, Quadrupole,
Raman Spectroscopy, Reversed
Phase, Triple Quadrupole, UV-Vis
Spectroscopy,
Cell Sorting, Chemical Synthesis,
Biopsy, Blood, Human Cell Lines,
Enzymatic Assays, Fluorescence
Human Primary, Mice, Mouse, Urine,
Microscopy, Ion Exchange, LC, LC-MS
coupling, Light Microscopy, MALDI,
Multicolor Analysis, Normal Phase,
Nuclear Magnetic Resonance,
oligosaccharide profiling, QqTOF, Real
63
Institute for Cancer Studies
University of Birmingham
(United Kingdom)
Mass Spectrometry Facility
The School of Pharmacy
(United Kingdom)
School of Chemistry
University of Southampton
(United Kingdom)
Mapping of the Lipidome, Membrane
Microdomains, Membrane Trafficking,
Metabolomics of the Lipidome,
Pharmacology and toxicology of the
Lipidome, Proteomics of the Lipidome,
Analytical Tools in Lipidomics,
Bioinformatics related to the Lipidome,
Genomics of the Lipidome, Lipid
Metabolism, Lipid Signalling, Lipid
Transport, Lipids in Inflammation,
Mapping of the Lipidome, Membrane
Microdomains, Membrane Trafficking,
Metabolomics of the Lipidome,
Pharmacology and toxicology of the
Lipidome, Proteomics of the Lipidome,
Signalomics of the Lipidome,
Time PCR, Reversed Phase, Size
Exclusion, Solid Phase Extraction, Thin
layer chromatography, TOF,
Ceramides [SP02], Diradylglycerols
APCI, APPI, Capillary Electrophoresis, Biopsy, Blood, Human Cell Lines,
[GL02], Eicosanoids [FA03], Fatty Acids CI, Confocal Microscopy, dHPLC, DNA Human Primary, Mice, Mouse,
and Conjugates [FA01], Fatty alcohols Sequencing, EI, Enzymatic Assays,
[FA05], Glycerophosphates [GP10],
ESI, Fluorescence Microscopy, FTICR,
Glycerophosphocholines [GP01],
GC, GC-MS coupling, Gel
Glycerophosphoethanolamines [GP02], Electrophoresis, Immuno Assays, Ion
Glycerophosphoglycerols [GP04],
Exchange, Ion Trap, LC, LC-MS
Glycerophosphoglycerophosphates
coupling, Light Microscopy, MALDI,
[GP05], Glycerophosphoinositol
Microarrays, nano ESI, NCI, Normal
bisphosphates [GP08],
Phase, Nuclear Magnetic Resonance,
Glycerophosphoinositol
Quadrupole, Real Time PCR, Reversed
monophosphates [GP07],
Phase, Size Exclusion, Solid Phase
Glycerophosphoinositol trisphosphates Extraction, Surface Plasmon
[GP09], Glycerophosphoinositols [GP06], Resonance, Thin layer
Glycerophosphoserines [GP03],
chromatography, TOF, Triple
Monoradylglycerols [GL01], Oxidized
Quadrupole, Two dimensional
glycerophospholipids [GP20],
Electrophoresis, UV-Vis Spectroscopy,
Phosphosphingolipids [SP03], Sphingoid
bases [SP01],
Analytical Tools in Lipidomics, Lipid
Bile acids and derivatives [ST04],
ESI, Gel Electrophoresis, Ion
Biopsy, Blood, Human Cell Lines, Mice,
Metabolism, Lipid Signalling, Lipids and Isoprenoids [PR01], Polyprenols [PR03], Exchange, Ion Trap, LC, LC-MS
Mouse, Rats, Urine,
Nutrition, Mapping of the Lipidome,
Secosteroids [ST03], Steroid conjugates coupling, MALDI, nano ESI, Normal
Membrane Microdomains, Membrane [ST05], Steroids [ST02], Sterols [ST01], Phase, Nuclear Magnetic Resonance,
Trafficking, Metabolomics of the
QqTOF, Quadrupole, Reversed Phase,
Lipidome, Pharmacology and
Size Exclusion, Solid Phase Extraction,
toxicology of the Lipidome, Proteomics
TOF, Triple Quadrupole,
of the Lipidome, Signalomics of the
Lipidome,
Analytical Tools in Lipidomics,
CDP-Glycerols [GP13], Ceramides
Atomic Force, Biostatistics, Cell
Biopsy, Blood, Human Cell Lines,
Bioinformatics related to the Lipidome, [SP02], Diradylglycerols [GL02],
Sorting, Chemical Synthesis, Cluster
Human Primary, Mice, Yeast,
Chemical Synthesis of Lipids
Docosanoids [FA04], Eicosanoids
Analysis, Confocal Microscopy, Data
(Probes/Tools/Agents), Glycomics of
[FA03], Fatty aldehydes [FA06],
Processing, Database Setup, DNA
the Lipidome, Lipid Metabolism, Lipid Glycerophosphocholines [GP01],
Sequencing, Electron Microscopy,
Signalling, Lipid Transport, Lipids and Glycerophosphoethanolamines [GP02], Electron Spin Resonance, Enzymatic
Nutrition, Lipids in Inflammation,
Glycerophosphoglycerols [GP04],
Assays, ESI, Fluorescence Microscopy,
Mapping of the Lipidome, Membrane Glycerophosphoglycerophosphates
FTICR, Gel Electrophoresis, Immuno
Microdomains, Membrane Trafficking, [GP05],
Assays, Infrared Spectroscopy, LC, LCMetabolomics of the Lipidome,
Glycerophosphoglycerophosphoglycerols MS coupling, Light Microscopy, LiquidNutrigenomics of the Lipidome,
(Cardiolipins) [GP12],
Liquid Extraction, Microarrays,
Pharmacology and toxicology of the
Glycerophosphoinositol bisphosphates Multicolor Analysis, nano ESI, Near
Lipidome, Signalomics of the Lipidome, [GP08], Glycerophosphoinositol
Field, Normal Phase, Nuclear Magnetic
monophosphates [GP07],
Resonance, QqTOF, Quadrupole,
Glycerophosphoinositols [GP06],
Raman Spectroscopy, Real Time PCR,
64
Glycerophosphoserines [GP03],
Oxidized glycerophospholipids [GP20],
Oxygenated hydrocarbons [FA12],
Phosphosphingolipids [SP03],
Triradylglycerols [GL03],
Reversed Phase, SNP Analysis, Solid
Phase Extraction, Surface Plasmon
Resonance, Triple Quadrupole, UV-Vis
Spectroscopy,
Technology - Survey
United States
Institute
Areas of Lipidomics Expertise
Lipid Class - Survey
Department of Biochemistry
Wake Forest University School of
Medicine
(United States)
Lipid Metabolism, Lipid Signalling,
Lipids and Nutrition, Lipids in
Inflammation, Lipids in Microbiology,
Signalomics of the Lipidome,
Diradylglycerols [GL02], Docosanoids Biostatistics, Cell Sorting, Cluster
[FA04], Eicosanoids [FA03], Fatty Acids Analysis, Confocal Microscopy, Data
and Conjugates [FA01], Fatty ethers
Processing, Database Setup, DNA
[FA10], Glycerophosphates [GP10],
Sequencing, Electron Microscopy,
Glycerophosphoinositol bisphosphates Electron Spin Resonance, Enzymatic
[GP08], Glycerophosphoinositol
Assays, Fluorescence Microscopy, GC,
monophosphates [GP07],
Gel Electrophoresis, Immune Affinity,
Glycerophosphoinositol trisphosphates Immuno Assays, Infrared Spectroscopy,
[GP09], Glycerophosphoinositols
Ion Exchange, LC, Light Microscopy,
[GP06],
Liquid-Liquid Extraction, Microarrays,
Multicolor Analysis, Normal Phase,
Nuclear Magnetic Resonance, Raman
Spectroscopy, Real Time PCR,
Reversed Phase, Size Exclusion, SNP
Analysis, Thin layer chromatography,
Two dimensional Electrophoresis, UVVis Spectroscopy,
Organisms and cellular Systems Survey
Archaebacteria, Biopsy, Blood,
Eubacteria, Human Cell Lines, Human
Primary, Mice, Mouse, Rabbits, Rats,
Stool, Urine, Yeast,
65
Appendix 3: Contact details of user registered in LEP ordered by country and organisation (as per 01.01.2008)
Lastname
Firstname
Email
Organisation
Phone
ZIP
City
Barrantes
Francisco J.
rtfjb1@criba.edu.ar
054 2914861201
8000
Bahia Blanca C.C. 857
Argentina
muller
maria
vivimico@yahoo.com.br
054 2914861201
8000
Bahia Blanca C.C. 857
Argentina
Lohner
Karl
karl.lohner@oeaw.ac.at
Universidad Nacional del UNESCO Chair
Sur
Biophys. & Mol.
Neurobiol
Universidad Nacional del UNESCO Chair
Sur
Biophys. & Mol.
Neurobiol
Austrian Academy of
Inst. Biophysics & X-ray
Sciences
Structure Research /
Fct. Lipidomics
Company
Street
Country
++43-316-4120323
A-8045
Graz
Schmiedlstrasse 6
Austria
Prassl
Ruth
ruth.prassl@oeaw.ac.at
Austrian Academy of
Sciences
Inst. Biophysics & X-ray
Structure Research /
Fct. Lipidomics
++43-316-4120323
A-8045
Graz
Schmiedlstrasse 6
Austria
Weinberger
Klaus
klaus.weinberger@biocrates.com
BIOCRATES Life
Sciences
BIOCRATES Life
Sciences
++43-512-5798234216
6020
Innsbruck
Innrain 66
Austria
Hackl
Hubert
hubert.hackl@tugraz.at
Graz University of
Technology
Institute for Genomics
and Bioinformatics
++43-316-8735345
8010
Graz
Petersgasse 14
Austria
Pichler
Harald
harald.pichler@tugraz.at
Graz University of
Technology
Institute for Molecular
Biotechnology
++43-316-8734089
A-8010
Graz
Petersgasse 14/I
Austria
Daum
Guenther
guenther.daum@tugraz.at
Graz University of
Technology
Institute of Biochemistry, +43-316-873-6462
Cell Biology Group
A-8010
Graz
Petersgasse 12/2
Austria
Keller
Walter
walter.keller@uni-graz.at
Inst. of Chemistry
Structural biology
+43-316-3805423
8010
Graz
Heinrichstr. 28
Austria
Sinner
Frank
frank.sinner@joanneum.at
Joanneum Research
Institute of Medical
Technologies and
Health Mangement
++433168762103
8036
Graz
Auenbruggerplatz
20/3
Austria
Kostner
Gert,M.
gerhard.kostner@meduni-graz.at
Medical University Graz
Molecular Biology and
Biochemistry,
+433163804200
A-8010
Graz
Harrachgasse 21
Austria
Frank
Sasa
sasa.frank@meduni-graz.at
Medical University Graz
Molecular Biology and
Biochemistry,
+433163804200
A-8010
Graz
Harrachgasse 21
Austria
Kohlwein
Sepp D.
sepp.kohlwein@uni-graz.at
University of Graz
IMB Biochemistry Yeast Genetics Group
++43 316 380
5487
A8010
Graz
Schubertstr. 1
Austria
Allmaier
Guenter
guenter.allmaier@tuwien.ac.at
Vienna University of
Technology
Institute of Chemical
Technologies and
Analytics, Bio and
Polymer Analysis
++43 1 58801
15160
A-1060
Vienna
Getreidemarkt
9/164
Austria
Köfeler
Harald
harald.koefeler@klinikum-graz.at
ZMF/Medical University
Graz
Core Facility for Mass
Spectrometry
++43 (316) 38573005
8010
Graz
Stiftingtalstrasse
24
Austria
Van Veldhoven
Paul
paul.vanveldhoven@med.kuleuven.be
K.U.Leuven
Div. Pharmacology Dep. Mol. Cell Biology
++32-16-345801
B-3000
Leuven
Campus
Gasthuisberg,
Herestraat
Belgium
ELIfe Final Report
Thevissen
Karin
karin.thevissen@biw.kuleuven.be
Katholieke Universiteit
Leuven
CMPG-PFI
++32 16 32 96 88
3001
Heverlee
Kasteelpark
Arenberg 20
Belgium
roelants
ivo
ivo.roelants@lrd.kuleuven.be
Katholieke Universiteit
Leuven
CMPG-PFI
++32 16 32 96 88
3001
Heverlee
Kasteelpark
Arenberg 20
Belgium
Swinnen
Johannes
johan.swinnen@med.kuleuven.be
Katholieke Universiteit
Leuven
Laboratory for
Experimental Medicine
and Endocrinology
(LEGENDO)
++32-16-330533
3000
Leuven
Herestraat 49 bus
902
Belgium
Dotti
Carlos
carlos.dotti@med.kuleuven.be
VIB and Catholic
University of Leuven
Neuronal Differentiation
Unit
+32-16-330526
3000
Leuven
Herestraat 49
Belgium
Trindade
Vera Maria
Treis
vmtt@ufrgs.br
Departamento de
Bioquímica - ICBSUniversidade Federal do
rio Grande do Sul
Laboratório de
Bioquímica e Biologia
Celular de Lipídios
55-51-33-16-55-50
90.035003
Porto Alegre
Rua Ramiro
Barcelos 2600anexo
Brazil
Boggs
Joan
jmboggs@sickkids.ca
Hospital for Sick
Children
Research Institute,
Boggs Laboratory
1-416-813-5919
M5G1X8
Toronto
555 University Ave. Canada
Lehner
Richard
richard.lehner@ualberta.ca
University of Alberta
1-780-492-2963
T6G 2S2
Edmonton
Zanmin
zmhu@genetics.ac.cn
Institute of Genetics and
Developmental Biology,
Chinese Academy of
Sciences
86-10-64889783
100101
Beijing
328 Heritage
Medical Research
Centre
Datun
Road,Chaoyang
District
Canada
Hu
CIHR Group in
Molecular and Cell
Biology of Lipids
Lab 305
Mu
Huiling
hm@biocentrum.dtu.dk
The Technical University
of Denmark
Biochemistry and
Nutrition Group,
Biocentrum-DTU
+45 45252744
2800
Lyngby
Building 224, DTU
Denmark
Yki-Järvinen
Hannele
ykijarvi@cc.helsinki.fi
Department of Medicine
University of Helsinki
+358 50 563 6899
00020
Helsinki
PO Box 700
Finland
Kinnunen
Paavo
paavo.kinnunen@helsinki.fi
Institute of Biomedicine
Helsinki Biophysics &
Biomembrane Group
+358-9-19125400
POB 63
Finland
Käkelä
Reijo
reijo.kakela@helsinki.fi
University of Helsinki
Institute of
Biomedicine/Käkelä
Group
++358-919125409
FINHelsinki
00014
University
of
Helsinki
P.O. Box Helsinki
63
Haartmaninkatu
8/P.O. Box 63
Finland
Somerharju
Pentti
pentti.somerharju@helsinki.fi
University of Helsinki
Institute of
358-9-19125410
Biomedicine/Somerharju
group
00014
Helsinki
Haartmaninkatu
8/PL 63
Finland
Hiltunen
J. Kalervo
kalervo.hiltunen@oulu.fi
University of Oulu
Department of
Biochemisty
FIN90014
Oulu
POB 3000
Finland
Oresic
Matej
matej.oresic@vtt.fi
VTT Technical Research Quantitative Biology and ++358-20-720Centre of Finland
Bioinformatics
4491
FIN02044
VTT
Espoo
Tietotie 2, P.O. Box Finland
1500
+358-8-553 1150
67
China
ELIfe Final Report
Chardot
Thierry
thierry.chardot@grignon.inra.fr
Biological Chemistry
LaboratoryNational
Institute for Agronomic
Research (INRA)
Biological Chemistry
Laboratory
++33-1-30815474
78850
Thiverval
Grignon
BP 1
France
Colard
Odile
colard@ccr.jussieu.fr
CHU Saint-Antoine
INSERM-538
(biomembranes)
33-01-40011340
75571
PARIS 12°
27 rue Chaligny
France
Carriere
Frederic
carriere@ibsm.cnrs-mrs.fr
CNRS
Laboratory of
Enzymology at
Interfaces and
Physiology of Lipolysis
33 4 91 16 41 34
13402
Marseille
cedex 20
31 chemin Joseph
Aiguier
France
CHIMINI
Giovanna
chimini@ciml.univ-mrs.fr
CNRS INSERM
CIML
33 491269404
13288
marseille
France
He
Hai-Tao
he@ciml.univ-mrs.fr
CNRS INSERM
CIML
33 491269404
13288
marseille
Lessire
René
rene.lessire@biomemb.u-bordeaux2.fr
CNRS UMR 5200
Université V. Segalen
Bordeaux 2
Laboratoire de
+33 (0)5 57 57 10
Biogenèse Membranaire 45
33076
Bordeaux
cedex
parc sxcientific de
luminy
parc sxcientific de
luminy
146 Rue Léo
Saignat
LESSIRE
René
rene.lessire@biomemb.u-bordeaux2.fr
CNRS UMR 5200
Université V. Segalen
Bordeaux 2
Laboratoire de
+33 (0)5 57 57 10
Biogenèse Membranaire 45
33076
Bordeaux
cedex
146 Rue Léo
Saignat
France
Moreau
Patrick
pmoreau@biomemb.u-bordeaux2.fr
CNRS UMR 5200
Université V. Segalen
Bordeaux 2
Laboratoire de
+33 (0)5 57 57 10
Biogenèse Membranaire 45
33076
Bordeaux
cedex
146 Rue Léo
Saignat
France
MANETAPEYRET
Lilly
lilly.maneta-peyret@biomemb.ubordeaux2.fr
CNRS UMR 5200
Université V. Segalen
Bordeaux 2
Laboratoire de
+33 (0)5 57 57 10
Biogenèse Membranaire 45
33076
Bordeaux
cedex
146 Rue Léo
Saignat
France
bessoule
jean-jacques
jean-jacques.bessoule@biomemb.ubordeaux2.fr
CNRS UMR 5200
Université V. Segalen
Bordeaux 2
Laboratoire de
+33 (0)5 57 57 10
Biogenèse Membranaire 45
33076
Bordeaux
cedex
146 Rue Léo
Saignat
France
Domergue
Frédéric
frederic.domergue@u-bordeaux2.fr
CNRS UMR 5200
Université V. Segalen
Bordeaux 2
Laboratoire de
+33 (0)5 57 57 10
Biogenèse Membranaire 45
33076
Bordeaux
cedex
146 Rue Léo
Saignat
France
JEROME
JOUBES
jjoubes@biomemb.u-bordeaux2.fr
CNRS UMR 5200
Université V. Segalen
Bordeaux 2
Laboratoire de
+33 (0)5 57 57 10
Biogenèse Membranaire 45
33076
Bordeaux
cedex
146 Rue Léo
Saignat
France
Lambeau
Gerard
lambeau@ipmc.cnrs.fr
CNRS UMR6097
++33-(0)493957733
06560
Valbonne
Aleksander
edelman@necker.fr
Faculté de Médecine
René Descartes Paris 5
+33 1 40 61 56 21
75015
Paris
Sophia Antipolis660 route des
lucioles
156 rue de
Vaugirard
France
Edelman
Institute of molecular
and cellular
pharmacology
INSERM U467/IFR94
Proteomics Platform
Ollero
Mario
ollero@necker.fr
Faculté de Médecine
René Descartes Paris 5
INSERM U467/IFR94
Proteomics Platform
+33 1 40 61 56 21
75015
Paris
156 rue de
Vaugirard
France
France
France
France
68
ELIfe Final Report
STAAT
Frédéric
f.staat@iterg.com
Industrial Technical
Center
French Institute for Fats
and Oils (ITERG)
+33 5 56 36 00 44
33600
PESSAC
rue Monge
France
LAGARDE
Michel
Michel.Lagarde@insa-lyon.fr
INSA-Lyon and CNRS /
INSERM / Univ Lyon1
33-4-72 43 82 40
69621
Villeurbanne
20 Ave A. Einstein
France
GUICHARDANT Michel
Michel.Guichardant@insa-lyon.fr
INSA-Lyon and CNRS /
INSERM / Univ Lyon1
33-4-72 43 82 40
69621
Villeurbanne
20 Ave A. Einstein
France
Dugail
Isabelle
idugail@bhdc.jussieu.fr
INSERM
IMBL-Lipid Signaling
(UMR 585 INSERM /
INSA-Lyon)
IMBL-Lipid Signaling
(UMR 585 INSERM /
INSA-Lyon)
INSERM U671
++33142346923
75006
Paris
15 rue de l ecole
de medecine
France
Saulnier-Blache
Jean
Sébastien
saulnier@toulouse.inserm.fr
INSERM,
INSERM U586 (Obesity
Research Unit)
33-5-62172956
31432
Toulouse
CHU Rangueil
BP84225
France
Bertrand-Michel
Justine
justine.BertrandMichel@toulouse.inserm.fr
INSERM/Toulouse
Genopole
IFR30 Lipid Analysis
Platform
33 (0)561779414
31024
Toulouse
CPTP Bat C,
Hopital Purpan
BP3028
France
Nicaud
Jean-marc
jean-marc.nicaud@grignon.inra.fr
National Institute for
Agronomic Research
(INRA) and National
Centre for Scientific
Research (CNRS)
Microbiology and
Molecular Genetic
Laboratory, UMR2585
++33 130815450
F78850
ThivervalGrignon
CBAI B.P. 01
France
Leray
Claude
leraycl@wanadoo.fr
Private
Cyberlipid Center
330467414365
34090
Montpellier
2 rue du colonel
Marchand
France
Bougnoux
Philippe
bougnoux@med.univ-tours.fr
Université François
Rabelais
Nutrition, Croissance et
Cancer, INSERM E211
+33 (0)247 366179 37044
TOURS
CHU Bretonneau
France
Khan
Naim
Naim.Khan@u-bourgogne.fr
University
University of Bourgogne
+33 3 80 39 63 12
DIJON
6 Bd Gabriel
France
Vidal-Wagner
Juan
Juan.Vidal@eur.appliedbiosystems.com
Applera Deutschland
GmbH
Applied Biosystems
+49615196705231 64293
Darmstadt
Frankfurter Strasse Germany
129B
Berthold
Heiner
hkb@akdae.de
Arzneimittelkommission
der deutschen
Ärzteschaft
AkdÄ
++4930400456500 10623
Berlin
Herbert-LewinPlatz 1
Germany
Mueller
Martin J.
Martin.Mueller@biozentrum.uniwuerzburg.de
Biocenter, University of
Wuerzburg
Julius-v-Sachs-Institute
for Biosciences,
Pharmaceutical Biology
++49-931-8886160
D-97082
Wuerzburg
Julius-von-SachsPlatz 2
Germany
Krieg
Peter
p.krieg@dkfz.de
Deutsches
Eicosanoids and Tumor
Krebsforschungszentrum Development
49 6221 424506
D 69120
Heidelberg
Im Neuenheimer
Feld 280
Germany
Kleinhammer
Christian
c.kleinhammer@t-online.de
DGPF
Deutsche Gesellschaft
für Proteomforschung
089-1897 9007
82152
Martinsried
Am Klopferspitz 18
Germany
Gröne
Hermann-Josef h.-j.groene@dkfz.de
DKFZ(German Cancer
Research Center)
department of cellular
49-6221-424350
and molecular pathology
D-69120
Heidelberg
Im Neuenheimer
Feld 280
Germany
Beck
Renata
DKFZ(German Cancer
Research Center)
department of cellular
49-6221-424350
and molecular pathology
D-69120
Heidelberg
Im Neuenheimer
Feld 280
Germany
majussia@gmail.com
21000
69
ELIfe Final Report
Bernhard
Wolfgang
wolfgang.bernhard@med.unituebingen.de
Eberhard Karls
Universität Tübingen
Dept. of Neonatology,
Lipid Metabolism and
Developmental
Physiology Group
#49-(0)7071-2986377
72076
Tübingen
Calwerstr. 7
Germany
Bernhard
Wolfgang
wolfgang.bernhard@med.unituebingen.de
Eberhard Karls
Universität Tübingen
Dept. of Neonatology,
Lipid Metabolism and
Developmental
Physiology Group
#49-(0)7071-2986377
72076
Tübingen
Calwerstr. 7
Germany
Gesche
Jens
jgesche@web.de
Eberhard Karls
Universität Tübingen
Dept. of Neonatology,
Lipid Metabolism and
Developmental
Physiology Group
#49-(0)7071-2986377
72076
Tübingen
Calwerstr. 7
Germany
Feussner
Ivo
ifeussn@uni-goettingen.de
Georg-August-University
Albrecht-von-HallerInstitute for Plant
Sciences/Plant
Biochemistry
++49-551-39-5743
37077
Goettingen
Justus-von-Liebig
Weg 11
Germany
Göbel
Cornelia
cgoebel@uni-goettingen.de
Georg-August-University
Albrecht-von-HallerInstitute for Plant
Sciences/Plant
Biochemistry
++49-551-39-5743
37077
Goettingen
Justus-von-Liebig
Weg 11
Germany
Willumeit
Regine
regine.willumeit@gkss.de
GKSS Research Center
Department of
Macromolecular
Structure Research
++49-4152-871291
21502
Geesthacht
Max-Planck-Str. 1
Germany
Herrmann
Andreas
andreas.herrmann@rz.hu-berlin.de
Humboldt-University
Berlin
Institute of
Biology/Molecular
Biophysics
49 (0) 30 2093
8860
D-10115
Berlin
Invalidenstr. 42
Germany
Rosenau
Frank
f.rosenau@fz-juelich.de
Institute for Molecular
Enzyme Technology,
Heinrich-Heine
University Duesseldorf
Microbial Expression
Technology
+492461612947
52426
Juelich
Stetternicher Forst
Germany
Kovacic
Filip
f.kovacic@fz-juelich.de
Institute for Molecular
Enzyme Technology,
Heinrich-Heine
University Duesseldorf
Microbial Expression
Technology
+492461612947
52426
Juelich
Stetternicher Forst
Germany
Kovacic
Filip
f.kovacic@fz-juelich.de
Institute for Molecular
Enzyme Technology,
Heinrich-Heine
University Duesseldorf
Microbial Expression
Technology
+492461612947
52426
Juelich
Stetternicher Forst
Germany
70
ELIfe Final Report
Hausmann
Sascha
S.Hausmann@fz-juelich.de
Institute for Molecular
Enzyme Technology,
Heinrich-Heine
University Duesseldorf
Microbial Expression
Technology
+492461612947
52426
Juelich
Stetternicher Forst
Germany
Rothe
Michael
michael.rothe@lipidomix.de
LIPIDOMIX GmbH
LIPIDOMIX GmbH
++49-3076778940
D-13088
Berlin
Berliner Allee 261269
Germany
Adam
Olaf
olaf.adam@lrz.uni-muenchen.de
Ludwig-MaximiliansUniversity
Walther-Straub-Institute
+49-89-21807564
80336
Munich
Goethestr. 33
Germany
Doermann
Peter
doermann@mpimp-golm.mpg.de
Max Planck Society
Max Planck Institute of
Molecular Plant
Physiology/Plant Lipid
Group
++49-331-5678259
14476
Golm
Am Mühlenberg 1
Germany
Schiller
Jürgen
Juergen.Schiller@medizin.uni-leipzig.de
Medical Faculty,
University of Leipzig
Institute of Medical
Physics and Biophysics
++49-3419715733
04107
Leipzig
Härtelstrasse 1618
Germany
Zschörnig
Olaf
zsco@medizin.uni-leipzig.de
Medical Faculty,
University of Leipzig
Institute of Medical
Physics and Biophysics
++49-3419715733
04107
Leipzig
Härtelstrasse 1618
Germany
Frentzen
Margrit
frentzen@bio1.rwth-aachen.de
RWTH Aachen
University
Institute for Biology I,
Botany
+49-(0)2418026641
52074
Aachen
Worringerweg 1
Germany
Sadre
Radin
rsadre@bio1.rwth-aachen.de
RWTH Aachen
University
Institute for Biology I,
Botany
+49-(0)2418026641
52074
Aachen
Worringerweg 1
Germany
Reinartz
Andrea
andrea.reinartz@rwth-aachen.de
RWTH-Aachen
Institute for Pathology,
University Hospital
Áachen
++492418089729
52074
Aachen
Pauwelstr. 30
Germany
Sandhoff
Konrad
sandhoff@uni-bonn.de
Universität Bonn
Kekulé Institut f.
++49 228 735346
Organische Chemie und
Biochemie
53121
Bonn
Gerhard-DomagkStraße 1
Germany
van EchtenDeckert
Gerhild
g.echten.deckert@uni-bonn.de
University Bonn
Kekulé-Institute for
Organic Chemistry and
Biochemistry
++49-228-732703
53121
Bonn
Gerhard-DomagkStr. 1
Germany
Füllekrug
Joachim
Joachim.Fuellekrug@med.uniheidelberg.de
University Heidelberg
Department of
Gastroenterology
(+49) 6221
5638960
69120
Heidelberg
INF 410
Germany
Ceglarek
Uta
uta.ceglarek@medizin.uni-leipzig.de
University Hospital
Leipzig
Insitute of Laboratory
Medicine, Clinical
Chemistry and
Molecular Diagnostics
++49-3419722407
04103
Leipzig
Liebigstraße 27
Germany
Höning
Stefan
shoening@uni-koeln.de
University of Cologne
Institute of Biochemistry
I
++49-2214783656
50931
Cologne
Joseph-Stelzmann- Germany
Str. 52
Sperling
Petra
sperling@botanik.uni-hamburg.de
University of Hamburg
Biocenter Klein
Flottbekand Botanical
Garden/Plant
Physiology
+49-(0)40-42816343
D-22609
Hamburg
Ohnhorststr. 18
Germany
71
ELIfe Final Report
Jahreis
Gerhard
Gerhard.Jahreis@uni-jena.de
University of jena
Institut of Nutrition
++49-03641949610
D-07743
Jena
Dornburger Str. 24
Germany
Humpf
Hans-Ulrich
humpf@uni-muenster.de
University of Muenster
Institut for Food
Chemistry
+49 251 83 33867
48149
Muenster
Corrensstr. 49
Germany
Seedorf
Udo
seedorfu@uni-muenster.de
University of Münster
Leibniz-Institute of
Arteriosclerosis
Research
+49 251 8356181
48149
Münster
Domagkstr. 3
Germany
Robenek
Horst
robenek@uni-muenster.de
University of Münster
Leibniz-Institute of
Arteriosclerosis
Research
+49 251 8356181
48149
Münster
Domagkstr. 3
Germany
Klucken
Jochen
jochen.klucken@web.de
93051
Regensburg
Universitätsstr. 84
Germany
Gerd
gerd.schmitz@klinik.uni-regensburg.de
Department of
Neurology
Institute for Clinical
Chemistry
+49 941 9410
Schmitz
University of
Regensburg
University of
Regensburg
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Liebisch
Gerhard
gerhard.liebisch@klinik.uni-regensburg.de University of
Regensburg
Institute for Clinical
Chemistry
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Grandl
Margot
margot.grandl@klinik.uni-regensburg.de
University of
Regensburg
Institute for Clinical
Chemistry
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Böttcher
Alfred
alfred.boettcher@klinik.uni-regensburg.de University of
Regensburg
Institute for Clinical
Chemistry
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Sigruener
Alex
alexander.sigruener@klinik.uniregensburg.de
University of
Regensburg
Institute for Clinical
Chemistry
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Leidl
Katharina
katharina.leidl@klinik.uni-regensburg.de
University of
Regensburg
Institute for Clinical
Chemistry
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Schmitz-Madry
Anna
anna.schmitz-madry@klinik.uniregensburg.de
University of
Regensburg
Institute for Clinical
Chemistry
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Valis
Linda
linda1.valis@klinik.uni-regensburg.de
University of
Regensburg
Institute for Clinical
Chemistry
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Kischel
Jette
jette.kischel@klinik.uni-regensburg.de
University of
Regensburg
Institute for Clinical
Chemistry
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Mages
Josef
josef.mages@klinik.uni-regensburg.de
University of
Regensburg
Institute for Clinical
Chemistry
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Wagenknecht
Daniela
daniela.wagenknecht@klinik.uniregensburg.de
University of
Regensburg
Institute for Clinical
Chemistry
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Wycislo
Matthias
matthias.wycislo@klinik.uniregensburg.de
University of
Regensburg
Institute for Clinical
Chemistry
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Lehneis
Stefan
stefan.lehneis@klinik.uni-regensburg.de
University of
Regensburg
Institute for Clinical
Chemistry
++49-941-9446201
93053
Regensburg
Franz-JosefStrauss-Allee 11
Germany
Naim
Hassan Y.
hassan.naim@tiho-hannover.de
University of Veterinary
Medicine Hannover
Department of
Physiological Chemistry
++49-511-953
8780
D-30559
Hannover
Bünteweg 17
Germany
72
ELIfe Final Report
Lekka
Marilena E.
mlekka@cc.uoi.gr
University of Ioannina
Laboratory of Lipid
Analysis
+3026510-98367
451 10
Ioannina
Panepistemioupolis Greece
Rühl
Ralph
rruehl@indi.biochem.dote.hu
Department of
Biochemistry and
Molecular Biology,
University of Debrecen
Laboratory of Nutritional
Bioactivation and
Bioanalysis
+36 30 320 97 69
4012
Debrecen
Nagyerdei Krt. 98
Hungary
Vigh
Laszlo
vigh@brc.hu
Laboratory of Molecular
Stress Biology
+36-62-432-048
H-6726
Szeged
Temesvari krt. 62.
Hungary
Balogh
Gabor
Baloghg@BRC.HU
Laboratory of Molecular
Stress Biology
+36-62-432-048
H-6726
Szeged
Temesvari krt. 62.
Hungary
L. Kiss
Anna
Kissa@ana2.sote.hu
Institute of Biochemistry,
Biological Research
Centre, HUng. Acad.
Sci.
Institute of Biochemistry,
Biological Research
Centre, HUng. Acad.
Sci.
Semmelweis University
Dept of Human
Morphology and
Developmental Biology
36 1 215
6924/3610
1094
Budapest
Tuzolto u. 58
Hungary
Szabo
Gabor
szabog@dote.hu
University of Debrecen
Dept. Biophysics and
Cell Biology
++36-52-455866
4010
Debrecen
Egyetem ter 1.
Hungary
Matyus
Laszlo
lmatyus@jaguar.dote.hu
University of Debrecen
Dept. Biophysics and
Cell Biology
++36-52-455866
4010
Debrecen
Egyetem ter 1.
Hungary
Goda
Katalin
goda@jaguar.unideb.hu
University of Debrecen
Dept. Biophysics and
Cell Biology
++36-52-455866
4010
Debrecen
Egyetem ter 1.
Hungary
Porath
Amiram
amiramp@post.tau.ac.il
Tel Aviv University
972-3-6407842
69978
Tel Aviv
Levanon
Israel
Futerman
Tony
tony.futerman@weizmann.ac.il
Weizmann Institute
Sackler School of
Medicine-Minerva
Center
Dept of Biological
Chemistry
++972-8-9342704
76100
Rehovot
1 Herzl Street
Israel
Chatgilialoglu
Chryssostomos chrys@isof.cnr.it
Consiglio Nazionale
delle Ricerche
ISOF/BioFreeRadicals
++39-051-6398309
40129
Bologna
Via Piero Gobetti
101
Italy
MEGLI
Francesco M.
f.m.megli@biologia.uniba.it
Dept Biochemistry and
Molecular Biology
EPR Lipidomics
++39 080 5443367 70126
BARI
V. E. Orabona, 4
Italy
Greco
Anita
agreco@iss.it
Dept of Cell Biology and
Neurosciences
Istituto Superiore di
Sanità
++39-0649903087
00161
ROME
viale Regina Elena, Italy
299
Vaccaro
Anna Maria
annamaria.vaccaro@iss.it
Istituto Superiore di
Sanita
Anna Maria Vaccaro
Unit of Physiopatology
of genetic diseases
++39 06 4990
2416
00161
Roma
Viale Regina Elena
299
Italy
Luini
Alberto
dcbo@negrisud.it
Mario Negri Sud Institute Department of Cell
Biology and Oncology
+39 0872570353
66030
Santa Maria
Imbaro
Via Nazionale 8/A
Italy
Corda
Daniela
corda@negrisud.it
Mario Negri Sud Institute Department of Cell
Biology and Oncology
+39 0872570353
66030
Santa Maria
Imbaro
Via Nazionale 8/A
Italy
Masserini
Massimo
massimo.masserini@unimib.it
University MilanoBicocca
++39-02-64488203
20052
Monza
Via Cadore, 48
Italy
Biochemistry group
73
ELIfe Final Report
Miserocchi
Giuseppe
massimo.masserini@unimib.it
University MilanoBicocca
Department of
Experimental Medicine
++39-02-64488203
20052
Monza
Via Cadore, 48
Italy
Pitto
Marina
marina.pitto@unimib.it
University MilanoBicocca
Department of
Experimental Medicine
++39-02-64488203
20052
Monza
Via Cadore, 48
Italy
Camici
Guido
camici@scibio.unifi.it
University of Florence
Dipartimento di Scienze
Biochimiche
++390554598328
50143
Firenze
Viale G.B.
Morgagni 50
Italy
ALBI
ELISABETTA
EALBI@UNIPG.IT
University of Perugia
Department of Clinical
and Experimental
Medicine,
Physiopathology,
University School of
Medicine
++39-0755729085
06100
Perugia
Policlinico
Monteluce, via
Brunamonti
Italy
Aveldaño
Marta Isabel
avelda@criba.edu.ar
University of Perugia
Department of Clinical
and Experimental
Medicine,
Physiopathology,
University School of
Medicine
++39-0755729085
06100
Perugia
Policlinico
Monteluce, via
Brunamonti
Italy
Corazzi
Lanfranco
corazzi@unipg.it
University of Perugia
Department of Internal
Medicine
0039 75 5857423
06122
Perugia
Via del Giochetto
Italy
Goracci
Gianfrancesco
goracci@unipg.it
University of Perugia
Department of Internal
Medicine, Section of
Biochemistry
++39-075-5857420
I-06122
Perugia
Via del Giochetto
Italy
Corazzi
Lanfranco
corazzi@unipg.it
University of Perugia
Department of Internal
Medicine, Section of
Biochemistry
++39-075-5857420
I-06122
Perugia
Via del Giochetto
Italy
Funato
Koichi
kfunato@hiroshima-u.ac.jp
Hiroshima University
Funato Group
++81-82-424-7925
739-8528
HigashiHiroshima
1-4-4,
Kagamiyama
Japan
Tojo
Hiromasa
htojo@mr-mbio.med.osaka-u.ac.jp
Osaka University
Graduate School of
Medicine
Department of
Biochemistry and
Molecular Biology
++81-6-6879-3283
662-0018
Suita
2-2 Yamadaoka
Japan
Asano
Chiyuki
asayuki@mr-mbio.med.osaka-u.ac.jp
Osaka University
Graduate School of
Medicine
Department of
Biochemistry and
Molecular Biology
++81-6-6879-3283
662-0018
Suita
2-2 Yamadaoka
Japan
Yasugi
Etsuko
e-yasugi@umin.net
The University ofTokyo
Department of
Metabolome, Graduate
School of Medicine
81-3-5841-3651
113-0033
Tokyo
7-3-1, Hongo,
Bunkyo-ku
Japan
Kulik
Willem
w.kulik@amc.uva.nl
Academic Medical
Center
Laboratory Genetic
Metabolic Diseases
+31-20-5669111
NL-1100
DD
Amsterdam
P.O. Box 22660
Netherlands
Houtkooper
Riekelt
r.h.houtkooper@amc.uva.nl
Academic Medical
Center
Laboratory Genetic
Metabolic Diseases
+31-20-5669111
NL-1100
DD
Amsterdam
P.O. Box 22660
Netherlands
74
ELIfe Final Report
van Lenthe
Henk
H.vanLenthe@amc.uva.nl
Academic Medical
Center
Laboratory Genetic
Metabolic Diseases
+31-20-5669111
NL-1100
DD
Amsterdam
P.O. Box 22660
Netherlands
van der Hoeven
Rob
rob.hoeven-van-der@dsm.nl
analysis/spectrometry
DSM
0031 152792278
2600 MA
Delft
p.o. box 1
Netherlands
Demacker
Pierre
P.Demacker@aig.umcn.nl
Dept Internal Medicine,
UMC Nijmegen
Lipid laboratory
-
6500 HB
Nijmegen
Geert Grooteplein
zuid 8
Netherlands
Schmidt
Thomas
schmidt@physics.leidenuniv.nl
Leiden Institute of
Physics, Leiden
University
Physics of Life
Processes
+31 71 527 5982
2333 CA
Leiden
Niels Bohrweg 2
Netherlands
Pool
Wim
pool@nioz.nl
NIOZ
NIOZ
31 222 369582
1790 AB
Den Burg
P.O. Box 59
Netherlands
Kahya
Nicoletta
nicoletta.kahya@philips.com
Philips
Philips Research,
Molecular Diagnostics
+31-40-2743794
5656 AE
Eindhoven
High Tech Campus Netherlands
4
Leeflang
Bas R.
b.r.leeflang@chem.uu.nl
Utrecht University
Bijvoet Center
++31-30-2533498
Utrecht
Padualaan 8
Netherlands
Egmond
Maarten
m.r.egmond@chem.uu.nl
Utrecht University
Bijvoet Center
++31-30-2533498
Utrecht
Padualaan 8
Netherlands
van Meer
Gerrit
g.vanmeer@chem.uu.nl
Utrecht University
Bijvoet Center
++31-30-2533498
Utrecht
Padualaan 8
Netherlands
GómezFernández
de Kroon
Juan C.
jcgomez@um.es
Utrecht University
Bijvoet Center
++31-30-2533498
Utrecht
Padualaan 8
Netherlands
Toon
a.i.p.m.dekroon@chem.uu.nl
Utrecht University
Bijvoet Center
++31-30-2533498
NL-584
CH
NL-584
CH
NL-584
CH
NL-584
CH
NL-584
CH
Utrecht
Padualaan 8
Netherlands
de Cock
J.J.P.A. (Hans)
IB.deCock@bio.uu.nl
Utrecht University
+31-30-2536616
3584 CH
Utrecht
Padualaan 8
Netherlands
van Meer
Gerrit
g.vanmeer@chem.uu.nl
Utrecht University
+31-30-2536616
3584 CH
Utrecht
Padualaan 8
Netherlands
Breukink
Eefjan
e.j.breukink@chem.uu.nl
Utrecht University
+31-30-2536616
3584 CH
Utrecht
Padualaan 8
Netherlands
Brouwers
Jos
j.brouwers@vet.uu.nl
Utrecht University
+31-30-2536616
3584 CH
Utrecht
Padualaan 8
Netherlands
Rottier
Peter
p.rottier@vet.uu.nl
Utrecht University
+31-30-2536616
3584 CH
Utrecht
Padualaan 8
Netherlands
Rodenburg
Kees W.
k.w.rodenburg@bio.uu.nl
Utrecht University
+31-30-2536616
3584 CH
Utrecht
Padualaan 8
Netherlands
Burger
Koert
K.N.J.Burger@bio.uu.nl
Utrecht University
+31-30-2536616
3584 CH
Utrecht
Padualaan 8
Netherlands
van der Horst
Dick
D.J.vanderHorst@bio.uu.nl
Utrecht University
Institute of
Biomembranes
Institute of
Biomembranes
Institute of
Biomembranes
Institute of
Biomembranes
Institute of
Biomembranes
Institute of
Biomembranes
Institute of
Biomembranes
Institute of
Biomembranes
+31-30-2536616
3584 CH
Utrecht
Padualaan 8
Netherlands
Helms
Bernd
j.b.helms@vet.uu.nl
Utrecht University
+31-30-2536616
3584 CH
Utrecht
Padualaan 8
Netherlands
Kersten
Sander
sander.kersten@wur.nl
Wageningen University
Institute of
Biomembranes
Nutrition, metabolism
and genomics group
++31-317-485787
6703 HD
Wageningen
Bomenweg 2
Netherlands
Skotland
Tore
tore.skotland@ge.com
GE Healthcare
Preclinical sciences
+47 2318 5666
0401
Oslo
Nycoveien 2
Norway
75
ELIfe Final Report
Sandvig
(adm./leader of
research group)
Kirsten
ksandvig@radium.uio.no
The Norwegian Radium
Hospital
Inst. for Cancer
Research/Sandvigs
group:Intracellular
transport
47 22934294
0310
Oslo
Montebello
Norway
Pulaski
Lukasz
lpulaski@cbm.pan.pl
Centre for Medical
Biology PAS
++48-42-2723639
93-232
Lodz
Lodowa 106
Poland
Macri
Beatrice
beatrice_macri@yahoo.com
Faculty of Biology,
University of Bucharest
Laboratory of
Transcriptional
Regulation
Department of Animal
Physiology and
Biophysics
00-40-21-318 15
69
050095
Bucharest
Splaiul
Independentei, 9195
Romania
Svetina
Sasa
sasa.svetina@biofiz.mf.uni-lj.si
University of Ljubljana
Institute of Biophysics,
Faculty of Medicine
-386 1 5437600
1000
Ljubljana
Lipiceva 2
Slovenia
GAMARRO
FRANCISCO
gamarro@ipb.csic.es
CONSEJO SUPERIOR
DE INVESTIGACIONES
CIENTÍFICAS
0034958181667
18100
ARMILLAGRANADA
Parque
Tecnológico de
Ciencias de la
Salud. Avda. del
Conocimiento s
Spain
Fabrias
Gemma
gfdqob@cid.csic.es
CSIC
INSTITUTO DE
PARASITOLOGIA Y
BIOMEDICINA LOPEZNEYRA, Working
Group:
BIOCHEMISTRY AND
MOLECULAR
PHARMACOLOGY. Dr.
FRANCISCO
GAMARRO
IQAC/Research Unit on
BioActive Molecules
34-93-4006115
08034
Barcelona
Jordi Girona 18
Spain
Goñi
Félix M.
felix.goni@ehu.es
CSIC and Universidad
del País Vasco
Unidad de Biofísica
++34-94-601-2625
48940
Leioa
Sarriena s
Spain
Mañes
Santos
smanes@cnb.uam.es
Spanish Research
Council
National Center of
Biotechnology
++34-91-5854840
28049
Madrid
Spain
Merida
Isabel
imerida@cnb.uam.es
Spanish Research
Council
National Center of
Biotechnology
++34-91-5854840
28049
Madrid
Balsinde
Jesús
jbalsinde@ibgm.uva.es
Spanish Research
Council & University of
Valladolid School of
Medicine
Institute of Molecular
Biology and Genetics
+34-983-423-062
E-47003
Valladolid
Darwin, 3. Campus
Cantoblanco
University
Darwin, 3. Campus
Cantoblanco
University
Calle Sanz y Fores
s
Perez-Gil
Jesus
perejil@bbm1.ucm.es
Universidad
Complutense
BIOMIL (BIOphysics of
Membranes and
Lipid/Protein Interfaces)
++34-91-3944994
28040
Madrid
Fac. Biologia,
Dept. Bioquimica
Spain
Perillo
María
mperillo
Universidad
Complutense
BIOMIL (BIOphysics of
Membranes and
Lipid/Protein Interfaces)
++34-91-3944994
28040
Madrid
Fac. Biologia,
Dept. Bioquimica
Spain
76
Spain
Spain
ELIfe Final Report
GómezFernández
Juan C.
jcgomez@um.es
University of Murcia
Research Group in
Biomembranes,
Department of
Biochemistry and
Molecular Biology
++34-968364766
E-30100
Murcia
Facultad de
Veterinaria,
Campus de
Espinardo
Spain
Ochoa
Begoña
begona.ochoa@ehu.es
University of the Basque
Country
Dept. Physiology,
Medical School
++34946012846
48940
Bilbao
Sarriena
Spain
RUIZ-LARREA
M. BEGOÑA
mbego.ruizlarrea@ehu.es
University of the Basque
Country
Dept. Physiology,
Medical School
++34946012846
48940
Bilbao
Sarriena
Spain
Ruiz-Sanz
Jose Ignacio
joseignacio.ruizs@ehu.es
University of the Basque
Country
Dept. Physiology,
Medical School
++34946012846
48940
Bilbao
Sarriena
Spain
Martínez
María José
mariajose.martinez@ehu.es
University of the Basque
Country
Dept. Physiology,
Medical School
++34946012846
48940
Bilbao
Sarriena
Spain
Jacobsson
Anders
anders.jacobsson@wgi.su.se
Stockholm University
The Wenner-Gren
Institute
++46-8-164127
SE10691
Stockholm
Arrhenius lab. F3
Sweden
Nedergaard
Jan
jan@metabol.su.se
Stockholm University
The Wenner-Gren
Institute
++46-8-164127
SE10691
Stockholm
Arrhenius lab. F3
Sweden
Parmryd
Ingela
ingela.parmryd@cellbio.su.se
Stockholm University
The Wenner-Gren
Institute
++46-8-164127
SE10691
Stockholm
Arrhenius lab. F3
Sweden
Carlsson
Anders
anders.carlsson@vv.slu.se
Swedish University of
Agricultural Sciences
Department of Crop
Science
please fill in
23053
Alnarp
Box 44
Sweden
Lindblom
Göran
goran.lindblom@chem.umu.se
Umeå University
Department of
Biophysical Chemistry
+46907865228
SE90187
Umeå
Linaeus väg 10
Sweden
Geyer
Roland
roland.geyer@eur.appliedbiosystems.com Applera Europe B.V.
LC-MS Support
+41 41 799 7742
6343
Rotkreuz
Grundstrasse 10
Switzerland
Hopfgartner
Gérard
Gerard.Hopfgartner@pharm.unige.ch
Life Sciences Mass
Spectrometry
+41-22-3796344
1211
Geneva
Bd Yvoy 20
Switzerland
Varesio
Emmanuel
Emmanuel.Varesio@pharm.unige.ch
Life Sciences Mass
Spectrometry
+41-22-3796344
1211
Geneva
Bd Yvoy 20
Switzerland
De Libero
Gennaro
gennaro.delibero@unibas.ch
+41 61 2652365
4031
Basel
Hebelstrasse, 20
Switzerland
Conzelmann
Andreas
andreas.conzelmann@unifr.ch
University of Fribourg
University of Basel,
Experimental
Immunology
Division of Biochemistry
0041 26 300 8630
CH-1700
Fribourg
5, chemin du
musée
Switzerland
Schneiter
Roger
roger.schneiter@unifr.ch
University of Fribourg
Division of Biochemistry
0041 26 300 8630
CH-1700
Fribourg
Switzerland
Yu
John
johnyu@gate.sinica.edu.tw
Academia Sinica
Institute of Cellular and
Organismic Biology,
Section of Stem Cells
++886-227899531
11529
Taipei
5, chemin du
musée
Nankang
School of
Pharmaceutical
Sciences - University of
Geneva
School of
Pharmaceutical
Sciences - University of
Geneva
University Hospital,
Department of Research
Taiwan
77
ELIfe Final Report
CHUANG
Nin-Nin
zonnc@sinica.edu.tw
Academia Sinica
Institute of Cellular and
Organismic Biology,
Section of Stem Cells
++886-227899531
11529
Taipei
Nankang
Taiwan
Wu
Wen-guey
wgwu@life.nthu.edu.tw
National Tsing Hua
University
886-3-5742752
30043
Hinchu
Kung-Fu Rd
Taiwan
O Donnell
Valerie
o-donnellvb@cardiff.ac.uk
Cardiff University
Institute of
Bioinformatics and
Structural Biology
Dept. of Medical
Biochemistry &
Immunology, School of
Medicine
0044 29 2074
8447
CF14
4XN
Cardiff
Heath Park
United
Kingdom
Wase
Nishikant
cpp06nvw@shef.ac.uk
Chemical and Process
Chemical Engineering
Engineering University of Life Science Interface
Sheffield
+44-07809717355
S3 7RD
Sheffield
40 LeavyGreave
Road
United
Kingdom
Schweizer
Michael
M.Schweizer@hw.ac.uk
Heriot-Watt University
School of Life Sciences
Richard
r.templer@imperial.ac.uk
Imperial College London
in association with the
Institute of Cancer
Research and the
London Research
Institute of CRUK
The Chemical Biology
Centre in the
Department of
Chemistry
EH14
Edinburgh
4AS
SW7 2AZ London
Roccarton
Templer
++44 131 451
3186
+44 (0)20 7594
5787
United
Kingdom
United
Kingdom
Mullard
Asher
a.mullard@nature.com
NPG
Nature Reviews
Molecular Cell Biology
+44(0)2078433641 N1 9XW
Butters
Terry
terry.butters@bioch.ox.ac.uk
Oxford University
Glycobiology Institute
+44 1865 275725
van der Spoel
Aarnoud
aarnoud.vanderspoel@bioch.ox.ac.uk
Oxford University
Glycobiology Institute
Salisbury
Malcolm
Malcolm.Salisbury@shell.com
Shell Global Solutions
(UK)
Wierzbicki
Anthony
Anthony.Wierzbicki@kcl.ac.uk
Griffiths
William
Karu
London
Exhibition Road
4 Crinan St
United
Kingdom
OX1 3QU Oxford
South Parks Rd
United
Kingdom
+44 1865 275725
OX1 3QU Oxford
South Parks Rd
United
Kingdom
Shell Global Solutions
(UK)
+44-151-373-5730
CH13SH
Chester
P.O. Box 1
United
Kingdom
St Thomas Hospital
Refsum disease group
++442071881256
SE1 7EH
London
Lambeth Palace
Road
United
Kingdom
william.griffiths@ulsop.ac.uk
The School of Pharmacy
Mass Spectrometry
Facility
++44-2077535876
WC1N
1AX
London
29/39 Brunswick
Square
United
Kingdom
Kersti
kersti.karu@pharmacy.ac.uk
The School of Pharmacy
Mass Spectrometry
Facility
++44-2077535876
WC1N
1AX
London
29/39 Brunswick
Square
United
Kingdom
Scigelova
Michaela
michaela.scigelova@thermo.com
Thermo Electron
European application
laboratory
+44 1442233555
HP2 7GE
Hemel
Hempstead
1 Boundary Park
United
Kingdom
SMITH
TERRY
T.K.Smith@dundee.ac.uk
UNIV OF DUNDEE
WELLCOME TRUST
BIOCENTRE, SCHOOL
OF LIFE SCIENCES,
UNIV OF DUNDEE
++44-(0)1382388688
DD1 5EH
DUNDEE
HAWKHILL
United
Kingdom
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ELIfe Final Report
Talmud
Philippa
p.talmud@ucl.ac.uk
University College
London
Cardiovascular
Genetics,
+44 207 679 6968
Thies
Frank
f.thies@abdn.ac.uk
University of Aberdeen
Aberdeen lipidomix
group
Lloyd
Matthew
M.D.Lloyd@bath.ac.uk
University of Bath
Wakelam
Michael
m.j.o.wakelam@bham.ac.uk
Nicolaou
Anna
MASOODI
WC1E
6JF
London
5 University St
United
Kingdom
44 (0)1224 553020 AB25
2ZD
Aberdeen
Polwarth building,
Foresterhill
United
Kingdom
Department of
Pharmacy &
Pharmacology
University of Birmingham Institute for Cancer
Studies
++-44-12253867686
BA2 7AY
Bath
Claverton Down
United
Kingdom
44-(0)121-4143293
B15 2TT
Birmingham
Vincent Drive
United
Kingdom
a.nicolaou@bradford.ac.uk
University of Bradford
School of Pharmacy
++44-1274224717
BD7 1DP
Bradford
Richmond Road
United
Kingdom
MOJGAN
m.masoodi@bradford.ac.uk
University of Bradford
School of Pharmacy
++44-1274224717
BD7 1DP
Bradford
Richmond Road
United
Kingdom
Massey
Karen
k.massey@bradford.ac.uk
University of Bradford
School of Pharmacy
++44-1274224717
BD7 1DP
Bradford
Richmond Road
United
Kingdom
Mir
Adnan
A.A.Mir@bradford.ac.uk
University of Bradford
School of Pharmacy
BD7 1DP
Bradford
Richmond Road
Anyakoha
Ngozi
n.g.anyakoha@bradford.ac.uk
University of Bradford
School of Pharmacy
++44-1274224717
++44-1274224717
BD7 1DP
Bradford
Richmond Road
United
Kingdom
United
Kingdom
van Veen
Hendrik W.
hwv20@cam.ac.uk
University of Cambridge
Department of
Pharmacology
44-1223-334032
CB2 1PD
Cambridge
Tennis Court Road
United
Kingdom
Sansom
Mark
mark.sansom@bioch.ox.ac.uk
University of Oxford
Dept. of Biochemistry
+44 1865 275371
OX1 3QU Oxford
South Parks Road
United
Kingdom
Postle
Tony
adp@soton.ac.uk
University of
Southampton
School of Chemistry
++44 (0)2380
796161
SO17
1BJ
Scaife
Jes
jes.scaife@writtle.ac.uk
Writtle College
Centre for Equine and
Animal Science
44 1245 424200
CM1 3RR Chelmsford
Lordship Road
United
Kingdom
Chikunya
Sife
sifelani.chikunya@writtle.ac.uk
Writtle College
Centre for Equine and
Animal Science
44 1245 424200
CM1 3RR Chelmsford
Lordship Road
United
Kingdom
Moye-Rowley
Scott
scott-moye-rowley@uiowa.edu
Dept. of Molecular
Physiology and
Biophysics
University of Iowa
1-319-335-7874
52246
Iowa City
6-530 Bowen
Science Building
United
States
BARD
Martin
mbard@iupui.edu
Indiana Umiversity Purdue University
Indianapolis
Indiana Umiversity Purdue University
Indianapolis
317-274-0593
46202
Indianapolis
723 W. Michigan
St.
United
States
Yu
Robert
ryu@mcg.edu
Medical College of
Georgia
Institute of Molecular
Medicine and Genetics
01-706-721-0699
GA
30912
Augusta
1120 15th Street
United
States
Reid
Scott
reidsk@musc.edu
Medical University of
South Carolina
COBRE in Lipidomics &
Pathobiology
843-792-4323
29425
Charleston
PO Box 250509
United
States
Hama
Hiroko
hama@musc.edu
Medical University of
South Carolina
COBRE in Lipidomics &
Pathobiology
843-792-4323
29425
Charleston
PO Box 250509
United
States
Southampton University Road
United
Kingdom
79
ELIfe Final Report
Frohman
Michael
michael@pharm.stonybrook.edu
Stony Brook University
Center for
please fill in
Developmental Genetics
11794
Polt
Robin
polt@u.arizona.edu
The Polt Group
Dawn
dcotter@sdsc.edu
++01-520-6216322
858-822-3619
AZ 85721 Tucson
Cotter
The University of
Arizona
University of California
San Diego
92093
La Jolla
Fahy
Eoin
fahye@sdsc.edu
University of California
San Diego
San Diego
Supercomputer Center /
LIPID MAPS
Bioinformatics Core
858-822-3619
92093
La Jolla
9500 Gilman Drive, United
Mail Code 0412
States
Maer
Andreia
amaer@sdsc.edu
University of California
San Diego
San Diego
Supercomputer Center /
LIPID MAPS
Bioinformatics Core
858-822-3619
92093
La Jolla
9500 Gilman Drive, United
Mail Code 0412
States
Sud
Manish
msud@sdsc.edu
University of California
San Diego
San Diego
Supercomputer Center /
LIPID MAPS
Bioinformatics Core
858-822-3619
92093
La Jolla
9500 Gilman Drive, United
Mail Code 0412
States
Ordovas
Jose
jose.ordovas@tufts.edu
USDA-Human Nutrition
Research Center on
Aging at Tufts University
Nutrition and Genomics
++1-617-556-3102
02111
Boston
711 Washington St
United
States
McPhail
Linda
lmcphail@wfubmc.edu
Wake Forest University
School of Medicine
Department of
Biochemistry
please fill in
27157
WinstonSalem
Medicial Center
Boulevard
United
States
San Diego
Supercomputer Center /
LIPID MAPS
Bioinformatics Core
Stony Brook
438 CMM
United
States
Department of
Chemistry
9500 Gilman Drive,
Mail Code 0412
United
States
United
States
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ELIfe Final Report
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
LSSG-CT-2004-013032
ELIfe
European Lipidomics Initiative: Shaping the Life Sciences
Instrument: Specific Support Action
Thematic Priority: FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6
TOWARDS A EUROPEAN LIPIDOMICS PROGRAM
Plan for Using and Disseminating Knowledge
(extended version)
Final Report
2005-2007
Period covered: from 1/1/05 to 1/7/07
Start date of project: 1/1/05
Project coordinator name:
Project coordinator organisation name:
Date of preparation: 1/1/08
Duration: 2.5 years
Prof. Gerrit van Meer
Utrecht University
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ELIfe Final Report
Section 1 - Exploitable knowledge and its use
The objectives of the Specific Support Action were:
Objective 1: To network the field of Metabolomics and to seek alliances with relevant stakeholders
Objective 2: To link the field of Metabolomics to the genomics and proteomics initiatives.
Objective 3: To define a strategy for Metabolomics research, using Lipidomics as an example.
Objective 4: To initiate an Expertise Platform on Lipidomics
Objective 5: To hold both science-related as well as policy meetings
One product of the SSA is the participation of roughly one thousand scientists, clinicians and
industrial stakeholders in the ELIfe workshops and meetings, and the resulting networking between
them. A second product is the interactive Lipidomics Expertise Platform on the ELIfe website. A
third product is formed by the papers published in the international literature. As such there are no
exploitable results.
Section 2 – Dissemination of knowledge
Size of
Audience
Partner
involved
Dates
Type
Type of Audience
Countries
2005
Flyer
General
World
1
20-21 May
05
Workshop
Global
2,3
Conference
Lipid technology
Young Basic
Scientists
Europe
1,500
Conference
Industry
Europe
200
2,12
Workshop 3.1
Workshop 3.2
Conference
1.3.2
Conference
1.3.1
Cell Biologists
Clinicians/lipidologists
Europe
+US
Europe
155
80
1,3,7
6,11,14
Lipidologists
Europe
150
2,3,11,14
Industry
Europe
55
Conference 1.5
General
World
254
2,9
1 through
14
Meeting 3.3
General; industry
World
60
3-Oct-05
2005
2006
Advertorial
Interview
Interview
European Parliament
General scientist
General
Europe
Europe
World
2005
Paper
Paper
Paper
Paper
Paper
1
2
3
4
5
General
Basic scientists
General
General
Basic scientists
1,12
12
12
12
1
2006
Paper
Paper
Paper
Paper
Paper
1
2
3
4
5
Lipidol/industry
Lipidol/industry
General
Lipidol/industry
Lipidol/industry
5
1
2
12
12
3-6 Sept 05
25-28 Sept
05
3-7 June 06
5-6 Sept 06
5-6 Sept 06
1-4 Oct 06
21-25 Oct
06
23-24 Oct
06
3
2,12
1 through
14
1,12
12
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ELIfe Final Report
Paper
Paper
Paper
Paper
Paper
6
7
8
9
10
2007
Lipidomics Grant
2007
Survey
Lipidol/industry
General
Lipidol/clinicians
Lipidol/clinicians
Basic scientists
1
1,2,7
1
1-3,7,12
1,3
1,2,8,10,12
General
2
Conferences.
The conferences organized under the present SSA were:
1.5 ELIfe/Special FEBS meeting
- The general ELIfe meeting entitled 'New concepts in lipidology: from lipidomics to disease'
(Noordwijkerhout, NL, 21-25 October 2006) was cosponsored as a FEBS special meeting (AP6a)
and was attended by over 250 participants, (http://www.febslipid2006.chem.uu.nl/). The speaker
selection was performed by the FEBS and ELIFE organizing committee (Ben de Kruijff, Pete
Downes, Bernd Helms, Kai Simons, Gerrit van Meer (chair) and Felix Wieland), and the other
ELIfe members by a written procedure. The meeting hosted a short joint symposium between the
Nordrhein-Westfälische Akademie der Wissenschaften and the Dutch Royal Academy of Arts and
Sciences contributed by Konrad Sandhoff, Bonn.
- The meeting hosted the industry meeting (see under T3.3).
- Bernd Helms and Gerrit van Meer edited a special ELIfe issue of FEBS Letters under the title
"Lipidome and Disease" which was distributed at the meeting.
2.2 Mass spec workshop (Dresden, 20-21 May 2005)
- This was a successful workshop on mostly technical aspects of mass spectrometry with a final
discussion focusing on what is required to apply this technology to the broader field of lipidomics,
especially the cell biological and medical applications. The meeting was attended by mostly mass
spectroscopists. The American LIPID MAPS consortium was well-represented, and also the
Japanese LipidBank consortium was represented.
- In a meeting of the Executive Committee (van Meer, Schmitz, Spener, Simons) with the
International Lipids Classification and Nomenclature Committee (Ed Dennis, Chris Raetz, Bob
Murphy, Fritz Spener, Gerrit van Meer, Yousuke Seyama and Takao Shimizu), actually attended by
Ed Dennis, Masahiro Nishijima, Gerd Schmitz, Fritz Spener, Kai Simons, Ryo Taguchi, and Gerrit
van Meer, all agreed to use one common database number for any lipid. The number would be
preceeded by the name of the database in which the lipid was entered. LIPID MAPS will propose a
procedure for how to handle this in practice.
- The workshop was also visited by the EC project officer, Christina Kyriakopoulou, who confirmed
the importance of bringing the lipidomics initiative to fruition.
3.1 Workshop Dynamic Lipid Organization in Cells
The EMBO/ELIfe workshop "Dynamic Lipid Organization in Cells" was held in Bilbao, 3-7 June
2006 by Kai Simons, Gisou van der Goot, Felix Goñi and Gerrit van Meer.
Co-sponsoring by EMBO was requested and awarded. The workshop was advertised all
over Europe. The response was great and the workshop was a big success. The workshop was
attended by 120 participants and had 35 speakers from all over the world, but mostly from Europe.
There were plenary discussions, poster sessions and round tables. The poster sessions worked
extremely well with lots of discussions at each poster over the whole meeting.
Conclusions from the workshop were that lipidomics is now ripe for funding from the next
EC framework programme. The technology in mass spectrometry and also many different
biophysical techniques, such as fluorescence correlation spectroscopy and single molecule
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ELIfe Final Report
microscopy are paving the way for a exploration of the functions of lipids in normal cell physiology
and in disease. Europe has a very strong research base in this area, which needs to be defended.
The outcome of the workshop was used as a preparation for the definition of possible
spearpoints in the next EC framework programme. Questions that could be addressed are protein
lipid interactions in structure biology, a role of single lipids cholesterol rafts in cellular function and
disease, the role of lipid droplets in normal physiology and obesity (the latter topic has been worked
out in the LipidomicNet proposal which has been selected for funding under FP7).
3.2 Workshop Lipidomics and Health
As decided unanimously in the Kick-off meeting, the ELIfe workshop was held as a satellite to the
ICBL 2006 meeting in Pécs, 5-10 September 2006 (http://www.icbl2006.hu/). The ELIfe workshop
was organized by Balázs Sarkadi and Andras Varadi (local organizers), Gerd Utermann, and Pam
Fredman. It was organized in excellent collaboration with the ICBL organizer Laszlo Vigh, and
with the ICBL chair (and ELIfe member) Michel Lagarde who also managed to involve the
International Society for the Study of Fatty Acids and Lipids (ISSFAL).
3.3 Industry meeting
Within the ELIfe/FEBS Special Meeting “New Concepts in Lipidology: from Lipidomics to
Disease” two half-day sessions were devoted to “Industrial and Technological Aspects of
Lipidomics”, chaired by Dr. Ron Potman (Unilever, chair of ELIfe advisory board, president of
Euro Fed Lipid) and Dr. Fritz Spener (ELIfe board member, University of Graz). Speakers were
either from industry or applied-research oriented academic institutions. A third short session
“Lipidomics and Metabolomics” was chaired by Drs. Peter Downes and Fran Platt. Almost all
speakers were present at the Round Table Discussion chaired by Dr. Spener at the end of the second
half-day industrial session with more than 60 participants.
The public conferences to which ELIfe contributed were:
1.3 Symposia
- The speakers were asked to connect from their different background metabolomics/lipidomics
with genomics/proteomics, and where possible relate their talks to medically relevant issues and
industrial applications. The idea was to enthuse broad audiences of scientists from the life sciences
(ELSO), industry (ISF) and biomedicine/biochemistry (ICBL) for the new developments in
metabolomics/lipidomics. The ELIfe members had discussions on the continuation of the
lipidomics initiative in Frankfurt, August 2006 and February 2007.
1.3.1a The ELSO 2005 conference
The ELSO 2005 conference, 3-7 September in Dresden (D), was attended by 1,200 scientists,
amongst whom 40% PhD students. The program consisted of 6 plenary sessions, 21 minisymposia
(in 3 sessions of 7 parallel symposia), 3 poster sessions and 7 sub-group meetings. ELIfe
contributed Minisymposium 1: "Lipidomics", chaired by Pam Fredman and Gerrit van Meer. After
an introduction of the EC-funded European Lipidomics Initiative and scientific ELIfe presentations
by the chairs, the invited lecturer Kim Ekroos (Astra-Zeneca, Mölndal, Sweden) presented a lecture
entitled "Unravelling phospholipidomes and sphingolipidomes by lipid profiling". This was
followed by three presentations selected from the poster abstracts. The minisymposium was
attended by over 100 participants.
The purpose of having this session was to expose a broad audience of life scientists
including many young scientists to lipidomics from three different angles, basic (van Meer),
medical (Fredman) and industry (Ekroos) and to try and make them enthusiastic for this upcoming
field. This field can only develop in its whole breadth if young scientists from different disciplines
join in. The lecturers were available for discussion at this networking meeting for one more day.
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ELIfe Final Report
1.3.1b The 4th Euro Fed Lipid congress
- Unexpectedly, ELSO did not organize its meeting in 2006. Instead, ELIfe contributed a session to
the 4th Euro Fed Lipid Congress – Fats, Oils and Lipids for a Healthier Future, Madrid, Spain
(October 1-4, 2006). One 4 hour session was contributed on Lipid Mediators and Lipidomics,
chaired by Fritz Spener and Michel Lagarde (http://www.eurofedlipid.org/meetings/madrid/). This
was a meeting with an industrial character.
1.3.2a International Society for Fat Research
At the 26th World Congress and Exhibition of the International Society for Fat Research in Prague,
Czech Republic, 25-28 September 2005, hosted by the Czech Chemical Society and Euro Fed
Lipid: 26th ISF World Congress "Modern aspects of fats and oils--A fascinating source of
knowledge", the session on "Lipid Bioscience and Genomics" was sponsored by ELIfe. It was
chaired by Fritz Spener and J. Kas:
- Ben van Ommen, Project coordinator of NUGO (www.nugo.org/metabolomics), TNO Nutrition
and Food Research, Zeist, NL: "Food and health approached by lipidomics and nutrigenomics".
- David A. Bernlohr, University of Minnesota, Minneapolis, MN, USA: "Proteomics of lipid
oxidation in diabetes and obesity".
The International Society for Fat Research and the European Federation for the Science and
Technology of Lipids, like the American Oil Chemists Society have a strong industrial character,
meaning that their activities are industry oriented and are rarely attended by life scientists and
clinical scientists.
1.3.2b International Conference on the Bioscience of Lipids
- ELIfe contributed to the 47th International Conference on the Bioscience of Lipids (ICBL - ELIfe
- ILPS) joint meeting, 5-10 September, 2006 in Pécs, Hungary (http://www.icbl2006.hu). One full
day was dedicated to two joint ICBL-ELIfe sessions on Lipidomics and Membrane microdomains,
co-chaired by Gerd Schmitz, Gabor Balogh, Gerrit van Meer and Janos Szöllõsi.
In the joint ICBL–ELIfe session on Lipidomics, Markus Wenk of National University of
Singapore discussed lipidomics of host-pathogen interactions. Edward A. Dennis, University of
California at San Diego, La Jolla CA, USA, told about the progress in LIPID MAPS and eicosanoid
lipidomics. Gerd Schmitz of University of Regensburg, Germany, focussed on the effect of
differential raft regulation in human macrophages upon Ox-LDL and E-LDL loading.
In the session Membrane Microdomains, Janos Szollosi of the Hungarian Academy of
Sciences, Debrecen, Hungary, told that membrane microdomains are distinct molecular association
clusters with function properties. Gerhard Schütz of Johannes Keppler University Linz, Austria, had
a look onto the nanoscopic organization of the plasma membrane using single molecule
microscopy. Unfortunately, Akihiro Kusumi of Nagoya University could not attend the meeting.
His plenary talk was replaced by Toshihide Kobayashi, Riken, Japan. He introduced the
combination of different novel cholesterol probes with the capability to reveal the cholesterol
gradient in cell membranes. Finally, Gerrit van Meer of Utrecht University, The Netherlands,
discussed dynamic organization and unexpected function of typical raft lipids.
Papers
2005:
1.a Fahy, E., S. Subramaniam, H.A. Brown, C.K. Glass, A.H. Merrill, Jr., R.C. Murphy, C.R. Raetz,
D.W. Russell, Y. Seyama, W. Shaw, T. Shimizu, F. Spener, G. van Meer, M.S. Vannieuwenhze,
S.H. White, J. Witztum, and E.A. Dennis (2005) A comprehensive classification system for lipids.
J. Lipid Res. 46, 839-861.
1.b idem: (2005) Eur. J. Lipid Sci. Technol. 107, 337-364
2. Varfolomeyev, S., Efremenko, E., Beletskaya, I., Bertini, I., Blackburn, G.M., Bogdanov, A.,
Cunin, R., Eichler, J., Galaev, I., Gladyshev, V., O’Hagan, D., Haertle, T., Jarv, J., Karyakin, A.,
85
ELIfe Final Report
Kurochkin, I., Mikolajczyk, M., Poroikov, V., Sakharov, I., Spener, F., Voyer, N., and Wild, J.
(2005) Postgenomic chemistry (IUPAC Technical Report). Pure Appl. Chem. 77, 1641–1654.
3. Spener F. (2005) European Commission funds lipidomics project. Eur. J. Lipid Sci. Technol.
107, 1-2.
4. Spener F. (2005) Lipidomics and consequences: a new classification system for lipids. Eur. J.
Lipid Sci. Technol. 107, 277-278.
5. van Meer, G. (2005) Cellular Lipidomics. EMBO J. 24, 3159-3165.
2006:
1. Griffiths, W. (2006) Why steroidomics in brain? Eur. J. Lipid Sci. Technol. 108, 707–708.
2. Helms, B. (2006) Host-Pathogen interactions: Lipids grease the way. Eur. J. Lipid Sci. Technol.
108, 895–897.
3. Schmitz, G., Liebisch, G., Langmann, T. (2006) Lipidomic strategies to study structural and
functional defects of ABC-transporters in cellular lipid trafficking. FEBS Lett. 580, 5597-5610.
4. Spener, F., Kohlwein, S.D., and Schmitz, G. (2006) Lipid droplets and lamellar bodies – from
innocent bystanders to prime targets of lipid research for combating human diseases. Eur. J. Lipid
Sci. Technol. 108, 541-543.
5. Spener, F., Zechner, R., and Borlak, J. (2006) Is lipotoxicity an oxymoron? Eur. J. Lipid Sci.
Technol. 108, 625-627.
6. van Meer, G. (2006) How do sphingolipids and lipid rafts relate to pathology? Eur. J. Lipid Sci.
Technol. 108, 799–801.
7. van Meer, G., Leeflang, B.R., Liebisch, G,. Schmitz, G., Goni, F.M. (2007) The European
lipidomics initiative: enabling technologies. Methods Enzymol. 432, 213-232.
8. Helms, B. and van Meer, G., eds. (2006) Lipidome and Disease. FEBS Letters Special Issue.
FEBS Lett. 580, 5429-5610.
A special ELIfe issue of FEBS Letters "Lipidome and Disease", edited by Gerrit van Meer and
Bernd Helms, drawing attention to the general meeting and the ELIfe activities, distributed at the
ELife/FEBS meeting.
9. van Meer, G. and Spener, F. (Co-Chairs), Leeflang, B.R. (Secretary), Beisiegel, U., Bougnoux,
P., Goñi, F., Griffiths, W., Hartmann, T., Helms, B., Hoekstra, D., Julià-Sapé, M., Larijani, B.,
Moschetta, A., Mouritsen, O.G., Norata, G.D., Payrastre, B., Record, M., Schmitz, G., Simons, K.,
Tselepis, A., Vaz, W., Vigh, L., Voelker, D.R., Wakelam, M.J.O., and Wanders, R.J.A. (2008)
Structural Medicine II: the Importance of Lipidomics for Health and Disease, European Science
Foundation Policy Briefing, in press.
The policy briefing specifically acknowledges EC specific support action LSSG-CT-2004-013032.
10. van Meer, G. (main proposer), Malhotra, V., Marsh, M., Simons, K., van der Goot, G., Warren,
G. (2008) Membrane Architecture and Dynamics (EuroMembrane), EuroCore theme proposal. Call
to be launched in spring 2008.
Interviews:
L. Winckler (2005) Lipidomics. Laborjournal 12, 20-23.
A paper aimed at the general biomedical community and based on interviews with ELIfe
participants and their team members.
C.D. Hillyer (2006) Lipidomics: taking it one lipid at a time. Inform 17, 206
With initiatives and consortiums around the world garnering significant grant money for lipidomics
research, the field as a whole is poised to make an important contribution to the ever-increasing
pool of knowledge about the role of lipids in human disease and metabolism. Interview with Fritz
Spener and representatives of the US and Japanese Lipidomics Initiatives.
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ELIfe Final Report
Advertorial:
Parliament Magazine, issue 210 of October 3, 2005, entitled "Fat is Bad?", stressing the need for
financial support under FP7 for the area of metabolomics, and of lipidomics in particular.
Survey:
The data in the Lipidomics Expertise Platform database have been analyzed and the survey will be
published on the website (see Annex 2 of the second periodic report).
Other activities:
- 2,500 scientists with an interest in lipids have been approached by e-mail in March and August
2006 with an invitation to attend the ELIfe general meeting.
- Scientists outside the ELIfe consortium have been actively invited to take part in the ELIfe
discussions on the development of lipidomics in a broader context. The "White Paper" on "Enabling
Technologies for Studying the Genome, Proteome and Cytome of the Lipidome" has been made
publicly available in an interactive format on the LEP website (http://www.lipidomicsexpertise.de/). LEP-Wiki serves as the basis for defining areas within the lipidomics field where
research is expected to have the largest impact for society, especially in the form of health benefits.
- One issue is the prolongation of the European Lipidomics Initiative in the form of actual
collaborations. A further result of the ELIfe SSA was the formation of a European consortium to
submit in the first call of the FP7 a grant application for a large collaborative project (21 partners
including 4 SMEs, coordinated by G. Schmitz, Regensburg), entitled 'Lipid droplets as dynamic
organelles of fat deposition and release: Translational research towards human disease'
(LipidomicNet).
- Finally, the Eurocores theme proposal Euromembrane that was discussed at the first annual
meeting has been adopted by ESF.
Section 3 - Publishable results
All papers have been published.
87
ELIfe Final Report
88
ELIfe Final Report
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
LSSG-CT-2004-013032
ELIfe
European Lipidomics Initiative: Shaping the Life Sciences
Instrument: Specific Support Action
Thematic Priority: FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6
TOWARDS A EUROPEAN LIPIDOMICS PROGRAM
Plan for Using and Disseminating Knowledge
(publishable version)
Final Report
2005-2007
Period covered: from 1/1/05 to 1/7/07
Start date of project: 1/1/05
Project coordinator name:
Project coordinator organisation name:
Date of preparation: 1/1/08
Duration: 2.5 years
Prof. Gerrit van Meer
Utrecht University
89
ELIfe Final Report
Dates
Type
2005
2006
Paper
Paper
Paper
Paper
Paper
Paper
Paper
Paper
Paper
Paper
Paper
Paper
Paper
Paper
Paper
Paper
2005
2006
Size of
Audience
Partner
involved
Type of Audience
Countries
General
Basic scientists
General
Basic scientists
General
General
General
General
Lipidol/industry
Lipidol/industry
Lipidol/industry
Lipidol/industry
Lipidol/industry
Lipidol/clinicians
Basic Scientists
Lipidol/clinicians
World
1,12
12
12
1
2
1,2,7
1-14
12
5
1
12
12
1
1-3,7,12
1,3
1
Interview 17
Interview 18
General scientist
General
Europe
World
1,12
12
2005
2005
Flyer
Advertorial
General
European Parliament
Europe
Europe
1
1-14
2007
Survey
General
2006
2007
2005
2006
2008
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
2
Summary
Over 2005 and 2006, the specific support action 'the European Lipidomics Initiative (ELIfe)' has
resulted in four successful workshops where stakeholders from basic science, industry and medicine
met to discuss cell biological, technological, industrial and clinical aspects of lipidomics. In
addition, ELIfe contributed lipidomics sessions to four networking meetings, one on life sciences,
one on the bioscience of lipids and two industrial lipid meetings. These workshops and meetings
culminated in the concluding general meeting in October 2006, attended by a broad audience of 250
scientists. At the occasion of this meeting a special journal issue was published on 'Lipidome and
Disease' [16]. The results of the the specific support action were reported in a series of policy
papers in the scientific literature [4-6, 8-13], and a policy briefing on lipidomics and health of the
European Science Foundation [14]. In addition, ELIfe contributed to a number of technical papers
on lipid classification and data handling [1-3] and to a 500 page document entitled 'Enabling
technologies for studying the genome, proteome and cytome of the lipidome.' [7]. This document
will be presented on the Lipidomics Expertise Wiki Portal (LEP-Wiki): http://www-cgi.uniregensburg.de/Klinik/Klinische_Chemie/lipidWiki/, to allow a direct interaction with the scientists
in the field. Interviews with ELIfe members appeared in various journals [17,18].
A survey was prepared based on registrations in the Lipidomics Expertise Platform
www.lipidomics-expertise.de with the purpose of identifying stakeholders and providing insight
in the available expertise, which can be used for contacting specific centers for collaboration. A 260
page document entitled 'Lipid droplets and lamellar bodies as dynamic organelles connecting influx,
efflux, and storage of lipids: Translational research towards human disease' was prepared as the
90
ELIfe Final Report
basis for a grant proposal under FP7 by a number of ELIfe members plus other scientists. This
proposal 'LipidomicNet' was recently selected for funding. A EuroCore theme proposal under the
name EuroMembrane [15] has been accepted by the European Science Foundation and a call for
applications is expected for early 2008.
In summary, we believe that the European Lipidomics Initiative has created many opportunities for
crossover between basic science and medical and commercial applications, and that it has inspired
stakeholders to seek contact and establish strategic alliances. Because national funding agencies and
policy makers value the European dimension, the project will impact on both the European and the
national level in shaping policies and research activities, both in applied and fundamental research.
One such field is that of nutrition and health. Technology development will allow more detailed
analyses of lipid patterns in diseased and healthy persons, which will drive discussions with the
food industry concerning the potential positive and negative effects of different types of (lipid)
nutrition on human health with new health policies drawn up as a result.
References
[1.a]
[1.b]
[2]
[3]
[4]
[5]
[6]
[7]
[8]
[9]
[10]
[11]
[12]
[13]
[14]
Fahy, E., S. Subramaniam, H.A. Brown, C.K. Glass, A.H. Merrill, Jr., R.C. Murphy, C.R.
Raetz, D.W. Russell, Y. Seyama, W. Shaw, T. Shimizu, F. Spener, G. van Meer, M.S.
Vannieuwenhze, S.H. White, J. Witztum, and E.A. Dennis (2005) A comprehensive
classification system for lipids. J. Lipid Res. 46, 839-861.
idem: (2005) Eur. J. Lipid Sci. Technol. 107, 337-364
Varfolomeyev, S., Efremenko, E., Beletskaya, I., Bertini, I., Blackburn, G.M., Bogdanov,
A., Cunin, R., Eichler, J., Galaev, I., Gladyshev, V., O’Hagan, D., Haertle, T., Jarv, J.,
Karyakin, A., Kurochkin, I., Mikolajczyk, M., Poroikov, V., Sakharov, I., Spener, F., Voyer,
N., and Wild, J. (2005) Postgenomic chemistry (IUPAC Technical Report). Pure Appl.
Chem. 77, 1641–1654.
Spener F. (2005) Lipidomics and consequences: a new classification system for lipids. Eur.
J. Lipid Sci. Technol. 107, 277-278.
van Meer, G. (2005) Cellular Lipidomics. EMBO J. 24, 3159-3165.
Schmitz, G., Liebisch, G., Langmann, T. (2006) Lipidomic strategies to study structural and
functional defects of ABC-transporters in cellular lipid trafficking. FEBS Lett. 580, 55975610.
van Meer, G., Leeflang, B.R., Liebisch, G,. Schmitz, G., Goni, F.M. (2007) The European
lipidomics initiative: enabling technologies. Methods Enzymol. 2007;432, 213-232.
White paper on 'Enabling technologies for studying the genome, proteome and cytome of
the lipidome.' to be published in Wikipedia format on the LEP website.
Spener F. (2005) European Commission funds lipidomics project. Eur. J. Lipid Sci. Technol.
107, 1-2.
Griffiths, W. (2006) Why steroidomics in brain? Eur. J. Lipid Sci. Technol. 108, 707–708.
Helms, B. (2006) Host-Pathogen interactions: Lipids grease the way. Eur. J. Lipid Sci.
Technol. 108, 895–897.
Spener, F., Kohlwein, S.D., and Schmitz, G. (2006) Lipid droplets and lamellar bodies –
from innocent bystanders to prime targets of lipid research for combating human diseases.
Eur. J. Lipid Sci. Technol. 108, 541-543.
Spener, F., Zechner, R., and Borlak, J. (2006) Is lipotoxicity an oxymoron? Eur. J. Lipid Sci.
Technol. 108, 625-627.
van Meer, G. (2006) How do sphingolipids and lipid rafts relate to pathology? Eur. J. Lipid
Sci. Technol. 108, 799–801.
van Meer, G. and Spener, F. (Co-Chairs), Leeflang, B.R. (Secretary), Beisiegel, U.,
Bougnoux, P., Goñi, F., Griffiths, W., Hartmann, T., Helms, B., Hoekstra, D., Julià-Sapé,
M., Larijani, B., Moschetta, A., Mouritsen, O.G., Norata, G.D., Payrastre, B., Record, M.,
91
ELIfe Final Report
[15]
[16]
[17]
[18]
Schmitz, G., Simons, K., Tselepis, A., Vaz, W., Vigh, L., Voelker, D.R., Wakelam, M.J.O.,
and Wanders, R.J.A. (2008) Structural Medicine II: the Importance of Lipidomics for Health
and Disease, European Science Foundation Policy Briefing, in press.
van Meer, G. (main proposer), Malhotra, V., Marsh, M., Simons, K., van der Goot, G.,
Warren, G. (2008) Membrane Architecture and Dynamics (EuroMEMBRANE), EuroCORE
theme proposal. Call to be launched in spring 2008.
Helms, B. and van Meer, G., eds. (2006) Lipidome and Disease. FEBS Letters Special Issue.
FEBS Lett. 580, 5429-5610.
Winckler, L. (2005) Lipidomics. Laborjournal 12, 20-23.
Hillyer, C.D. (2006) Lipidomics: taking it one lipid at a time. Inform 17, 206-208.
Other:
ELIfe flyer
Advertorial in the Parliament Magazine, issue 210 of October 3, 2005, entitled "Fat is Bad?"
Survey:
The data in the Lipidomics Expertise Platform database have been analyzed and the survey will be
published on the website (see Annex 2 of the reports).
92
ELIfe Final Report
QuickTime™ and a
TIFF (Uncompressed) decompressor
are needed to see this picture.
LSSG-CT-2004-013032
ELIfe
European Lipidomics Initiative: Shaping the Life Sciences
Instrument: Specific Support Action
Thematic Priority: FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6
TOWARDS A EUROPEAN LIPIDOMICS PROGRAM
Appendix 13
Science and society reporting questionnaire
Appendix 15
Final reporting questionnaire on workforce statistics
Appendix 16
Socio-economic reporting questionnaire
Final Report
2005-2007
Period covered: from 1/1/06 to 1/7/07
Start date of project: 1/1/05
Project coordinator name:
Project coordinator organisation name:
Date of preparation: 1/1/08
Duration: 2.5 years
Prof. Gerrit van Meer
Utrecht University
93
Appendix 13 – Science and society reporting questionnaire
All projects
ELIfe Final Report
Science and Society Reporting Questionnaire
Introduction
FP6 was designed to focus, integrate, structure and strengthen the European Research
Area (ERA). The influence of science and technology on society was acknowledged when
the ERA was established and the importance of having a healthy dialogue between
science and society was recognised. This area now forms part of the policy to structure
the ERA under the heading Science and Society. It incorporates ethical, gender and
communications issues together with issues affecting education and youth and
governance.
This questionnaire has been compiled for FP6 Project Coordinators. It has been
designed to help coordinators respond to contractual reporting requirements (Article
II.10.3 of the contract states that consortia must engage with actors beyond the research
community) and to facilitate the monitoring of the science and society dimension in FP6.
The information gathered through this exercise will be confidential and will not be
disclosed to any third parties or used in any way that could be linked to individual
projects.
Please complete the questionnaire by ticking boxes or filling out information where
requested. It would be appreciated if as many questions as possible could be completed.
Please note that Part A will be completed automatically when the contract number is
entered.
A
General Information on Contractor
1
Contract Number:
2
Instrument:
Specific Support Action
3
Thematic Priority:
FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6
4
Title of Project:
European Lipidomics Initiative:
Shaping the Life Sciences
5
Name and Title of
Coordinator:
Prof. G. van Meer
6
Period Covered, Start Date: 1/1/05
LSSG-CT-2004-013032
End Date: 1/7/07
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ELIfe Final Report
€ 487,200
7
EC Contribution to project:
B
Ethics
8
Which (if any) of the following does your research project involve?
 Human beings
 Human biological samples
 Personal data
 Genetic information
 Animals
 Human embryos or human embryonic stem cells
 Non human primates and other animals
 None of the above
9
To what extent do you believe ethical issues are relevant to your research project?
 Not relevant
 Minor relevance
 Significant relevance
 Critical
10
Do you have Ethicists or others with considerable ethics experience involved in the
project?
 Yes
 No
11
Did your project have a separate EC ethical review?
 Yes
 No
12
How much (including the value of time spent, as well as paid-out costs) do you
estimate your project (when it is completed) will have spent on considering and
dealing with ethical issues?
€
5,000
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ELIfe Final Report
C
Gender (to be completed for all projects except IPs and NoEs)
13a Did you undertake Gender Equality Actions in your research project?
 Yes
 No
13b If no, why not?
 Not relevant
 Team not gender aware
 No budget
 Not supported (no will)
 Other:
13c If yes, which of the following actions did you carry out and how effective were
they?
Not at all
effective
 Design and implement an equal opportunity policy
 Implement mentoring schemes for women
 Family friendly working conditions
14
No
How much (including the value of time spent, as well as paid-out costs) do you
estimate your project (when it is completed) will have spent on considering and
dealing with gender issues?
€
D
 


Was there a gender dimension associated with the research content?
 Yes. If yes, please specify

15
Very
effective
-
Science Education, Training and Career Development
16a Does this project anticipate having a direct impact on the local economy?
 Yes
 No
16b If Yes, is the project:
 Stimulating employment
 Retaining highly trained personnel
 Creating possible spin-out/start-up companies
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ELIfe Final Report
17
Does your partnership employ and train researchers?
 Yes
 No
18
Does your project involve working with young people at schools?
 Yes
 No
19
Is there any education material being produced directly or indirectly by your
project?
 Yes
 No
20
How much (including the value of time spent, as well as paid-out costs) do you
estimate your project has spent on considering and dealing with Science
Education, Training and Career Development issues? € 5000
E
Engaging With Actors Beyond the Research Community
20a Is the project likely to generate outputs (expertise or scientific advice) which could
be used by policy makers?
 Yes
 No
20b If Yes, is this a primary or secondary objective of the project?
 Primary
 Secondary
21a Did your project engage in significant communication with the public before
research commenced?
 Yes
 No
21b Was the focus or methodology of your project modified in response to any
communication with the public?
 Yes
 No
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ELIfe Final Report
22
Does your project involve someone whose role is solely to communicate with the
public?
 Yes
 No
F
Use and dissemination
23
How many articles were published ?
13
In refereed journals:
24
Other journals:
5
How many patents have been applied for ?
25
How many other Intellectual Property Rights were applied for?
26
How many spin-offs were created? -
27
Have you issued press releases related to your project (and if so, how many)?
 Yes, number:
 No
28
2
Have you held media briefings? If so, how many, and on average roughly how
many journalists attended?
 Yes, number of

average number of journalists:
briefings:
No
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ELIfe Final Report
29a Roughly how many items covering your project in the printed press, on radio or
television can you identify?
21
Press:
Radio:
Television:
29b Roughly how many items were:
Specialist
Press:
18
Non-specialist
Press:
3
National
Press:
1
International
Press:
20
30a Was there on-line information about the project?
 Yes
 Specific web site
 No
30b Roughly how frequently has it been updated?
6 months
31
Do you have an e-mail mailing list to send news about the project? If so, how
many subscribers to the list are there?
 Yes, number of subscribers:
 No
2,500 scientists were addressed
32a Have you created or participated in an event (e.g. workshop, conference,
information day) in order to communicate with the public (not just other
researchers or the press)?
 Yes
 No Except for the contributions to 2 lipid congresses that covered lipid commerce and
consumer demands
32b Roughly how many people attended these events and learned about your project?
200
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ELIfe Final Report
33a Have you produced a video or DVD film about your project?
 Yes
 No
33b If so, how effective do you believe it has been in communicating with the public?
 Unable to assess





Completely ineffective
Mostly ineffective
Partially effective
Significantly effective
Extremely effective
34a Have you produced posters, flyers or brochures about your project?
 Yes
 No
34b If so, how effective do you believe they have been in communicating with the
public?
 Unable to assess





35
Completely ineffective
Mostly ineffective
Partially effective
Significantly effective
Extremely effective
In how many different languages were these products (video/DVD, posters, flyers,
brochures) produced?
English
36
How have you distributed these products (video/DVD, posters, flyers, brochures)?
Please tick all methods you have used.
 Sent on request
 Sent to schools/academic institutions
 Distributed through government agencies/public buildings/libraries etc.
 Sent to potentially interested non-governmental bodies (NGOs, citizen’s associations etc)
 Other: Spread through the mailings of international oragnizations: FEBS, EMBO, EuroFed
Lipid, ICBL
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ELIfe Final Report
G Total Communication Spend
37
How much (including the value of time spent, as well as paid-out costs) do you
estimate your project (when it is completed) will have spent on communication
activities (engaging with the public, use and dissemination) as described in the
current questionnaire?
€
63,600
12 months of EC funded efforts; mostly
publications, website and meeting organization
H Comments
38
If you have any comments about your experience of meeting the Science and
Society objectives within your project, or any suggestions of improvements to the
programme please add them here:
In hindsight the initiative should have hired one full time person to handle the organizational aspects of
the initiative and the broad public dissemination of the results.
you for your heour help!
[Submission instructions will need to be elaborated by those that set up the questionnaire on
the Internet].
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ELIfe Final Report
Appendix 15 – Final reporting questionnaires on workforce statistics
All projects except IPs and NoEs
103
ELIfe Final Report
WORK FORCE STATISTICS
FINAL REPORT
This report is part of the final reporting to be completed by the contractors of all projects except IPs
and NoEs at the end of the project.
1. GENERAL INFORMATION
1.1. Contract No.: LSSG-CT-2004-013032
1.2. Thematic priority: FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6
1.3. Instrument type: Specific Support Action
1.4. Project acronym: ELIfe
1.5. Period covered (Start Date – End Date)1: 1/1/05 – 1/7/07
1.6. Name and title of co-ordinator1: Prof. G. van Meer
1.7. Name and title of contractor: Utrecht University
2. SCIENTIFIC LEADERSHIP AND MANAGEMENT, AND WORKFORCE STATISTICS FOR THE
PROJECT TO BE COMPLETED BY CONTRACTORS (SUMMARY TABLE)
Please complete the table below on a Headcount basis (Previously supplied data will be inserted
automatically)
Type of Position
Scientific manager
Scientific team leader / work package
manager
Experienced researcher (> 4 years)
Early researcher (<= 4 years)
PhD students
Technical staff
Other
Number of
Women
Number of
Men
Total
%
Women
%
Men
1
1
2
50
50
2
41
12
68
14
109
14
38
100
62
1
100
1
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ELIfe Final Report
Appendix 16 – Socio-economic reporting questionnaire
All projects
105
SOCIO-ECONOMIC REPORTING QUESTIONNAIRE
SUMMARY STATEMENT BY COORDINATOR
INTRODUCTION
In the process of building the European Research Area, democratic governance must ensure that social and
economic issues are taken into consideration in the research activities and that citizens are informed about
and aware of the social aspects with regard to scientific and technological progress. In this context, it is also
acknowledged that the benefits of research in support of socio-economic policy challenges would be
enhanced by an appropriate integration of socio-economic research dimensions.
The importance of the integration of socio-economic aspects in research was recognised in FP6 and should
be duly taken into consideration by contractors where relevant for the actions concerned in horizontal and
thematic activities of FP6.
This questionnaire applies to all projects and must be filled in by each contractor in the project. It is designed
to facilitate the monitoring of the integration of the socio-economic dimensions in FP6 and to finally support
the assessment of the research that will guide the future policy formulations and decisions.
The submission of this questionnaire will be done on-line. The details of the procedure to be used will be
communicated by the Commission to the project coordinator in due time.
The information gathered through this exercise will be kept confidential and will not be disclosed to any
third parties or used in any way that could be linked to individual projects.
QUESTIONS
1.1 Do your tasks in the project include socio-economic research activities1 ?
N/A
1.2 If “Yes”, what is the estimated total budget allocation that addresses
these activities ?
N/A
1
- Ex-ante or ex-post assessments (or contribution to such analysis e.g. cost-benefit/cost-effectiveness studies, etc…) of
the expected impact of the knowledge and/or technology generated from the research (project, programme or
framework programme), as well as analysis of the factors that would influence their exploitation (e.g. statistical
indicators, standardisation, ethical and regulatory aspects, impact on consumers and markets, public
awareness/acceptance and understanding of science, political/societal and/or economic implications, etc…)
- Any type of models or tools to support the assessment of impact on society, economy and businesses resulting from
the deployment of new services or technologies.
- Any research seeking both a better integration of Science in Society and Society in Science.
- Any type of research aiming at understanding the societal and economic phenomena (research in social sciences and
humanities)
- Actions e.g. assessments, tools & methods, comparative research, etc to support the formulation and implementation
of Community policies.
- Any type of activity involving scientist(s) with a specific background in social, political sciences or in economy
(discipline approach).
ELIfe Final Report
2.1 Do your tasks in the project include foresight methods2 ?
Not really
Only expected needs and developments in networking, technology and bioinformatics were discussed.
2.2 If “Yes”, what is the estimated total budget allocation that addresses
these activities?
3. How many person/months (estimated) were allocated to researchers
with a background in social sciences3, to perform your tasks for
the project ?
N/A
None
2
- Any type of foresight, i.e. participative vision-building approaches, future studies and forward looking activities,
including scenarios of the evolution of Europe’s potential in a related field, forecasting, prospective studies, forward
looks, etc.
3
Domains of academic disciplines covered by the social sciences are: Psychology, Economics, Education sciences,
Anthropology (social and cultural) and ethnology, Demography, Geography (human, economic and social), Town
and country planning, Management, Law, Linguistics, Political sciences, Sociology, Organisation and methods,
Miscellaneous social sciences and interdisciplinary.
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