PI Name:_____________________
Date:___________
9.1 Viral vectors:
QUESTION
Vector 1
Vector 2
Vector 3
Vector 4
Type of virus that makes
up the vector system?
Name of vector
construct?
Source of nucleic acid to
be inserted?
Name of inserted nucleic
acid?
Function of inserted
nucleic acid?
Discuss risks associated
with this inserted nucleic
acid if it were
accidentally transferred to
an unintended host
(e.g. is it oncogenic,
immunogenic, toxic,
immune suppressive,
angiogenic, an allergen,
etc)
Is insertional mutagenesis
a concern?
Why or why not?
List any key expression
control elements
(amphotropic
envelope gene, human
promoter, etc.)
List ALL host(s) the
vector will be transferred
into-include
genus/species where
applicable (e.g.
packaging cell line, cell
lines, prokaryotes, plants,
animals, insects, etc):
How will it be transferred
to each host
(transfection, viral
9.1 Recombinant/Synthetic Nucleic Acid ver. 09/13
PI Name:_____________________
Date:___________
transduction,
transformation, infection,
particle bombardment,
injection, etc)?
How much will be
transferred to each host
(concentration/titer and
volume)?
What percentage of the
viral genome will be
transferred to the host(s)?
Is the virus capable of
integrating into the host
genome?
Is the virus replication
defective?
If yes, describe
< 1/2
½ - 2/3
> 2/3
Other:
< 1/2
½ - 2/3
> 2/3
Other:
< 1/2
½ - 2/3
> 2/3
Other:
< 1/2
½ - 2/3
> 2/3
Other:
If no, provide
justification for the use of
a replication
competent virus
Will you use defective
viral vector in the
presence of helper virus?
If yes, specify which
List all helper plasmids
used to produce
recombinant virus
Has the vector
preparation been tested
(e.g. by commercial
vendor, another PI) or
will it be tested in your
lab for the presence of
replication competent
virus (RCV)?
If yes, describe the
method used for testing
and attach data as a
separate sheet if
available:
9.1 Recombinant/Synthetic Nucleic Acid ver. 09/13
PI Name:_____________________
Date:___________
Discuss safety features
of the viral vectors you
will be using (e.g. gene
deletions, expression of
packaging genes on
multiple plasmids, selfinactivating long terminal
repeats, limited tissue
tropism).
How did you obtain the
viral vector?
a.
Bought a commercial
kit Yes
No
a.
Which:
Bought a commercial
kit Yes
No
a.
Which:
Bought a commercial
kit Yes
No
a.
Which:
Bought a commercial
kit Yes
No
Which:
b.
Made all components
in my lab
Yes
No
b.
Made all components
in my lab
Yes
No
b.
Made all components
in my lab
Yes
No
b.
Made all components
in my lab
Yes
No
c.
Assembled in my lab
from components
made/obtained
elsewhere
Yes
No
c.
Assembled in my lab
from components
made/obtained
elsewhere
Yes
No
c.
Assembled in my lab
from components
made/obtained
elsewhere
Yes
No
c.
Assembled in my lab
from components
made/obtained
elsewhere
Yes
No
Obtained from:
d.
Received packaged
virus
Yes
No
Obtained from:
d.
Obtained from:
e.
Received transduced
cells
Yes
No
Obtained from:
Received packaged
virus
Yes
No
Obtained from:
d.
Obtained from:
e.
Received transduced
cells
Yes
No
Obtained from:
Received packaged
virus
Yes
No
Obtained from:
d.
Obtained from:
e.
Received transduced
cells
Yes
No
Obtained from:
Received packaged
virus
Yes
No
Obtained from:
e.
Received transduced
cells
Yes
No
Obtained from:
Attach a map of all viral constructs at the end of the document
9.1 Recombinant/Synthetic Nucleic Acid ver. 09/13