HIBBING COMMUNITY COLLEGE
COURSE OUTLINE
COURSE TITLE & NUMBER: MLT 1420: Laboratory Techniques
CREDITS: 4 (Lecture Hours 34 / Lab Hours 68 per Semester)
PREREQUISITES: None
CATALOG DESCRIPTION: Laboratory Techniques is an introductory course in
basic medical laboratory techniques. The equipment and techniques to be
studied include laboratory glassware, pipetting, balances, centrifuges, solution
chemistry, titration, spectrophotometry, and basic laboratory mathematics. This
is the first in a series of clinical chemistry courses designed to teach fundamental
concepts in clinical laboratory procedures.
OUTLINE OF MAJOR CONTENT AREAS:
I. Laboratory glassware
II. Dilutions
A. Calculations involving one dilution
B. Volume dilutions
C. Concentration dilutions
D. Serial dilutions
III. Molarity and Normality
IV. Solution Chemistry
A. Weight per unit weight (w/w)
B. Weight per unit volume (w/v)
C. Volume per unit volume (v/v)
V. Conversion from one expression of concentration to another
VI. Colorimetry
A. Beer’s law
B. Relationship between absorbance and transmittance
C. Linear and semilog graphs
D. Standard curves
COURSE GOALS/OBJECTIVES/OUTCOMES:
The student will
1.
match various types of laboratory glassware with its appropriate use.
2.
define the following terms; sample volume, diluent volume, total
volume, dilution factor.
3.
describe the procedure for making a series of tube dilutions.
4.
determine the concentration of any tube in a serial dilution.
Hibbing Community College, a technical & community college
an equal opportunity educator & employer
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calculate final concentration when performing serial dilutions.
compare and contrast dilutions and titers.
calculate the amount of one solution needed to make a solution of a
lesser concentration.
describe the difference between molar and normal solutions and be
able to calculate how to prepare solutions of given volume and
normality or molarity.
convert molar solution concentrations to normal solution
concentrations.
make a percent solution.
use Beer’s law to calculate concentration of unknowns.
convert between absorbance and transmittance values.
describe the current use of graphs and standard curves in the clinical
laboratory.
calculate the concentration of a substance using a standard curve.
obtain the concentrations of unknown by single standards and multiple
standards.
calculate the mean, median, and mode of a set of variables.
calculate the standard deviation and coefficient of variation of a set of
variables.
define the following terms: shift, trend.
apply prior knowledge to problem solving.
recognize abnormal or unusual test results.
recognize unacceptable quality control results.
apply knowledge of established procedures to verify test results
Lab Competencies
The student will
1.
demonstrate how to read the meniscus in volumetric flasks, pipettes,
and graduated cylinders.
2.
prepare solutions and dilutions as described in procedures and
manufacturer’s instructions.
1.
demonstrate an understanding of calculations using dilutions.
2.
perform serial dilution procedures.
3.
calculate the mean, standard deviation, and coefficient of variation for
a sample of test results
4.
calculate the upper and lower limits of acceptability for quality-control
test results.
5.
demonstrate safe and professional work habits.
HCC COMPETENCIES MET:
Working Productively & Cooperatively, Communicating Clearly & Effectively.
Hibbing Community College, a technical & community college
an equal opportunity educator & employer
STUDENT CONTRIBUTIONS:
Students are expected to attend all lectures and labs, complete assignments on
time, and spend necessary study time to pass all exams.
METHODS FOR EVALUATING STUDENT LEARNING:
Performance appraisals, assignment completion, lab reports, unit tests and a
final exam are the methods used to evaluate student learning.
SPECIAL INFORMATION: (SPECIAL FEES, DIRECTIVES ON HAZARDOUS
MATERIALS, TEXTBOOK USED, ETC.)
Students are expected to purchase lab coats and a calculator. Powder-free
gloves are supplied.
11/16/04
Date Approved
Hibbing Community College, a technical & community college
an equal opportunity educator & employer