E. coli BacPowerTM Guaranteed Package Quotation Form
Instructions
Please answer all of the following questions and send the completed form to protein@genscript.com if you choose the E. coli
BacPowerTM Guaranteed Package. Our service representative will contact you with a quote shortly.
Customer Information
If you have registered an account with GenScript, you can just identify yourself by giving us your name and email address or
Account No.
Name:
Account No.:
Phone:
Organization:
Shipping address:
(Necessary to determine
the shipping cost)
Email address:
Project Information
Nature of inquiry:
Order initiation:
Quote for ordering
Immediately
For pricing estimation
Within three months
For grant application
Beyond three months
Please read the following terms and conditions for the E. coli BacPowerTM Guaranteed Package:
Terms and Conditions: Protein sequences are subject to pre-project analysis. Exclusions are applicable for membrane proteins
and toxic proteins. For eligible proteins, a minimum of 3 mg soluble protein with >75% purity will be delivered to customers.
The protein service will be free of charge if the expression level is too low to eventually obtain 3 mg of purified protein after
Genscript’s evaluation. To increase the expression level of your protein, optimized genes are recommended using our
OptimumGeneTM technology (note: gene synthesis is at additional cost). Up to 3 fusion tag(s) may be tested to increase the
expression level and solubility of the protein and applied for protein production and purification. Fusion tags may be made
removable by introducing optional protease cleavage site between the tag and the target protein (note: Tag removal service is
available at extra cost). Expressed insoluble proteins will be subject to refolding using our FoldArt TM technology at no
additional fee.
Protein Expression & Purification
Protein name:
Species:
MW:
Protein sequence (crucial for evaluation) or accession number:
Note: Please enter the protein sequence if mutants are included in your protein sequence.
Gene synthesis with codon optimization (For Human/Mouse/Rat ORF genes exist in NCBI
database,starting from $0.29/bp; for other genes, starting from $0.35/bp)
(Please enter your gene sequence: )
Starting material:
Gene synthesis according to natural gene sequence (it is strongly recommended that
synthesized gene with optimized codon be used for protein expression)
(Please enter your gene sequence: )
Your own template DNA (it is strongly recommended that synthesized gene with optimized
codon be used for protein expression)
(Please attach vector map (e.g. NTI file), and enter your vector name:
, and gene
sequence:
Cytoplasmic protein
Periplasmic protein
Secreted protein
IgG antibody
Membrane-bound protein
Toxic to E. coli
Protein properties:
Tag position:
Note: If the target protein is membrane-bound or toxic to the bacterial host, please quote with E.
coli customized service.
Do you want GenScript to add the tag(s) at the N-terminus?
Yes
No, I have designated place(s) to add the tag(s) (Please specify):
Note: If internal tag(s) is/are involved, please designate the position number(s) that corresponds
to the sequence of which the accession number you have indicated above.
Please select up to three tags to facilitate the expression and purification of your target protein:
Tag selection:
Introducing Protease
cleavage site:
Protein characterization:
6xHis
GST
Other (please specify):
MBP
Trx
Sumo
Note: A customer selected tag or small tag (e.g. -6xHis) will be the first choice for protein
production. GenScript reserves the right to choose a best tag in order to guarantee the target
protein expression.
Do you want GenScript to add a protease cleavage site between the tags and the target protein?
Yes
No
If YES, please specify the protease cleavage site you want to add:
GenScript’s choice
Enterokinase
PreScission
rTev
Thrombin
Note: After protease cleavage, an extra 4-6 aa will be left of C-terminal tag and <2 aa of
N-terminal tag; therefore, we DO NOT recommend the introduction of protease cleavage site
between the target protein and the C-terminal tag.
SDS-PAGE & Western blot
SEC
ELISA
HPLC
MS
MALDI-TOF
N-terminal sequence
Note: SDS-PAGE & Western blot is FREE of charge.
Other comments
TF