Program Director/Principal Investigator (Last, First, Middle):
Freyer, James P.
BIOGRAPHICAL SKETCH
Provide the following information for the Senior/key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
James P. Freyer
Research Professor
eRA COMMONS USER NAME (credential, e.g., agency login)
FREYER
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, include postdoctoral training and
residency training if applicable.)
DEGREE
INSTITUTION AND LOCATION
MM/YY
FIELD OF STUDY
(if applicable)
La Salle College, Philadelphia, PA
University of Rochester, Rochester, NY
University of Rochester, Rochester, NY
Los Alamos National Laboratory, Los Alamos, NM
B.A.
M.S.
Ph.D.
PostDoc
05/72
05/78
12/81
02/84
Physics
Biophysics
Biophysics
Tumor Biology
A. Personal Statement
I have over 30 years experience in the use of 3-D in vitro models to investigate the effects of the tumor
microenvironment on tumor cell metabolism, proliferation, viability, therapy response and gene/protein
expression, as well as measurement of the chemical microenvironment within tissue models. I have
established expertise in effectively adapting and applying a wide variety of technologies to investigate the
tumor microenvironment, including: design, construction and application of cell culture systems; design,
construction and application of flow cytometry instrumentation; application of NMR spectroscopy and microimaging; design, validation and application of assays for measuring cellular metabolism and physiology;
design, validation and application of optical spectroscopy; and design, validation and application of
mathematical models of tumor development. I have 25 years experience as PI of NIH-funded projects, from
small R21 grants to large P41 technology centers. I have served as Co-Investigator on several other NIH R01
grants, as well as PI on NSF, private foundation and institutional grants. I have experience with scientific
management at several levels, from individual research teams to large (300) organizational units. I have
successfully collaborated with multi-disciplinary groups, and am currently involved in projects incorporating
physicists, biologists, electrical engineers, chemical engineers, synthetic chemists, optical spectroscopists,
mathematical modelers and computer scientists.
B. Positions and Honors
Positions and Employment
1976-1981 Graduate Fellow, Radiation Biology and Biophysics, University of Rochester.
1982-1984 Director’s Postdoctoral Fellow, Los Alamos National Laboratory.
1982-1986 Instructor in Pharmacology Department, University of New Mexico, Albuquerque, New Mexico,
teaching a self-designed course in Biosafety in the Laboratory.
1984-1987 Staff Member in Toxicology Group of Los Alamos National Laboratory; research on spheroid
biology, radiobiology, and flow cytometry.
1987-1999 Staff Member in Cell Biology Group of Los Alamos National Laboratory; research on spheroid
biology, radiobiology, flow cytometry, and NMR spectroscopy and imaging.
1998-now Adjunct Professor, Department of Cell Biology and Physiology, University of New Mexico.
1999-now Staff Member in Bioscience Division of Los Alamos National Laboratory; research on tumor
models, flow cytometry, optical tumor diagnosis and NMR spectroscopy and imaging.
2000-2001 Acting Deputy Division Director, Bioscience Division, Los Alamos National Laboratory.
2003-now Full Member, University of New Mexico Cancer Research and Treatment Center.
2007-2008 Group Leader, Advanced Measurement Sciences Group, Los Alamos National Laboratory.
2005-2010 Director and PI, National Flow Cytometry Resource.
2009-now Research Professor, Center for Biomedical Engineering, Department of Chemical and Nuclear
Engineering, University of New Mexico.
Program Director/Principal Investigator (Last, First, Middle):
Freyer, James P.
Other Experience and Professional Memberships
1985-2005 Member, Institutional Biosafety Committee, Los Alamos National Laboratory.
1988-2003 Chairperson of Institutional Biosafety Committee, Los Alamos National Laboratory.
1990-now Member of 12 NIH Site Review Committees.
1992-now Ad Hoc reviewer for numerous NIH Study Sections, including: Radiation; Diagnostic Radiology;
Biophysical Chemistry; Pathology B, Cell Biology SBIR, Biomedical Technology SBIR, Shared
Instrumentation, EUREKA and five other special review panels.
1996-now Ad Hoc Reviewer, National Cancer Institute of Canada.
1999-2002 Member of Radiation Study Section, Center for Scientific Review, NIH.
2001-2003 Co-Director and mentor, Research Experiences for Undergraduates program, NSF.
2004-2007 Mentor for students, National Science Foundation PUSH Program, with Northern New Mexico
Community College.
2007-now Lecturer in LANL qBio Summer School.
2008-now External Reviewer, Israeli Science Foundation.
Honors
1989-now
2002-2007
2002
2003-now
2007-now
2008-now
Associate Editor, International Journal of Radiation Oncology, Biology and Physics.
Associate Editor, Radiation Research.
Outstanding REU Program Mentor Award.
Full Member, University of New Mexico Cancer Research and Treatment Center.
Lecturer in LANL qBio Summer School.
Biology Councilor, International Society for Analytical Cytology.
C. Selected Peer-Reviewed Publications (from 85)
Most relevant to the current application
1. Freyer JP, Sutherland RM (1980) Selective dissociation and characterization of cells from different regions
of multicell tumor spheroids. Cancer Res. 40: 3956-3965. [PMC Journal - In Process]
2. Freyer JP, Sutherland RM (1986) Regulation of growth saturation and development of necrosis in
EMT6/Ro multicellular spheroids by the glucose and oxygen supply. Cancer Res. 46: 3504-3512. [PMC
Journal - In Process]
3. Freyer JP (1988) Role of necrosis in regulating the growth saturation of multicellular spheroids. Cancer
Res. 48: 2432-2439. [PMC Journal - In Process]
4. Freyer JP, Schor PL, Jarrett KA, Neeman M, Sillerud LO (1991) Cellular energetics measured by
phosphorous NMR spectroscopy are not correlated with chronic nutrient deficiency in multicellular tumor
spheroids. Cancer Res. 51: 3831-3837. [PMC Journal - In Process]
5. Kunz-Schughart LA, Habbersett RC, Freyer JP (1997) Mitochondrial distribution and activity in oncogenetransformed rat fibroblasts isolated from multicellular spheroids. Am. J. Physiol. 273: C1487-C1595.
6. Mourant JR, Hielscher AH, Freyer JP (1998) Evidence for intrinsic differences in light scattering properties
of malignant and nonmalignant cells. Cancer Cytopath. 84: 366-374. [PMC Journal - In Process]
7. Kunz-Schughart LA, Freyer JP (2002) Phosphorous metabolites and steady-state energetics of oncogenetransformed fibroblasts during three-dimensional growth. Am. J. Physiol. Cell. Physiol. 283: C1287-C1297.
[PMC Journal - In Process]
8. LaRue KEA, Kahlil M, Freyer JP (2004) Microenvironmental regulation of proliferation in EMT6 multicellular
spheroids is mediated through differential expression of cyclin-dependent kinase inhibitors. Cancer
Res.,64: 1621-1631. [PMC Journal - In Process]
9. Mourant JR, Short KW, Carpenter S, Kunapareddy N, Coburn L, Powers TM, Freyer JP (2005)
Biochemical differences in tumorigenic and non-tumorigenic cells measured by Raman and infrared
spectroscopy. J. Biomed. Optics 10: 31106:1-15. [PMC Journal - In Process]
10. Ramachandran J, Powers TM, Carpenter S, Garcia-Lopez A, Freyer JP, Mourant JR (2007) Light
scattering and microarchitectural differences between tumorigenic and non-tumorigenic cell models of
tissue. Optics Express 15: 4039-4053. [PMC Journal - In Process]
Additional recent publications of importance to the field (in chronological order)
1. Jiang Y, Pjesivac-Grbovic J, Cantrell C, Freyer JP (2005) Multiscale model of avascular tumor growth.
Biophys. J. 89: 3884-3894. [PMC1366955]
Program Director/Principal Investigator (Last, First, Middle):
Freyer, James P.
2. Naivar MA, Parson JD, Wilder ME, Habbersett RC, Edwards BS, Sklar L, Nolan JP, Graves SW, Martin JC,
Jett JH, Freyer JP (2007) Open, reconfigurable cytometric acquisition system: ORCAS. Cytometry 71A:
915-924. [PMC Journal - In Process]
3. Goddard GR, Brown LO, Habbersett R, Brady CI, Martin JC, Graves SW, Freyer JP, Doorn SK (2010) High
resolution spectral analysis of individual SERS-active nanoparticles in flow. J. Am. Chem. Soc. 132: 60816090. [PMC2861152]
4. Houston JP, Naivar MA, Freyer JP (2010) Digital acquisition of fluorescence lifetime by frequency domain
flow cytometry. Cytometry 77A: 861-872. [PMC Journal - In Process]
D. Research Support
CURRENT
1 R01 CA132629
10/21/2007 – 10/20/2012
NIH/NIDDK
Differential Metabolic Network Analysis of Tumor Progression
Role: Principal Investigator
The major goal of this project is to develop experimental and computational methods for characterizing
metabolic networks of cells growing in culture systems that serve as in vitro models of tumor progression.
2 P41 RR001315
7/1/1982 – 6/30/2012
NIH/NCRR
The National Flow Cytometry and Sorting Research Resource
Role: Project Principal Investigator
The major goals of this project are to develop new instrumentation and applications for flow cytometry. In
addition to the three R&&D Projects, this grant also incorporates: Collaboration and Service components to
provide technology to the biomedical community; a Training component; and a Dissemination component.
2 R01 CA071898
NIH/NCI
Light Scattering and Normal Tissue Models
Role: Co-Investigator
9/01/2008 – 8/31/2013
The major goal of this project is to develop fiber-optic probes for measuring light scatter from tissues and
apply them to cancer diagnostics using in vitro models and patients with cervical cancer.
COMPLETED
20070180ER
10/1/2006 – 9/30/2009
LANL LDRD/ER Program
Understanding a Killer: A Predictive Model of Tumor Development
Role: Co-Investigator
The major goal of this project is to develop a predictive, multiscale mathematical model of solid tumor
growth and development, and validate this model against experimental data obtained from multicellular
spheroids cultured under different nutrient conditions.
5 R01 EB003824
9/01/2004 – 8/31/2009
NIH/NIBIB
Raman Flow Cytometry for Diagnostics and Drug Discovery
Role: Co-Investigator
The major goal of this project is to develop instrumentation and reagents for the analysis of Raman-tagged
particles by flow cytometry, and apply these to multiplexed microsphere assays.