Program of the “IGEN Summer-school in Advanced Cytometry”
(updated 22nd June 2015)
Day I, Monday: 27.07.2015, LOCATION: Avsikten (close to entrance 65). Workshop participants
that may have it difficult to find the “Avsikten ” should congregate at the floor 10 of Cell Biology Building,
close to Entrance 64, and will be lead by organizers to the Avsikten .
Transport from Hotel du Nord will leave at 8.40.
9.00 – 9.15 Opening remarks (Emilia Wiechec)
9.15 – 9.30 Course participants introduce themselves.
9.30 - 10.15 “Flow cytometry – how it works, and how is it done; brief overview of other
equipment.” (Marek Los)
10.15 – 10.30 coffee break and informal discussions
10.30 – 11.15 “Laser-Scanning Cytometry, theoretical considerations, and examples of practical
applications – a perspective of an end-user” (Dr. Jerzy Grabarek, Univ. Szczecin, Poland – longterm user of LSC)
11.15 – 11.30 coffee break
11.30 – 12.00 Demonstration of configurations of iCys and Gallios cytometry (Grabarek, Wiechec,
Jain) equipment, and opportunity to book time with Dr. Grabarek for individual exercises on
iCys.
12.00 – 13.00 lunch break
LOCATION: Library of the core facility.
13.00 – 13.30 Lecture highlighting the capabilities of CyTOF2 (Mass-spectrometry cytometry)
13.30 – 14.00 Presentation and demo of the capabilities of CyTOF2 in groups of 3-4 students
14.00 – 17.00 Practical training on iCys – data acquisition and analysis of cell samples brought by
individual participants. Course-participants will have the opportunity to have their sample
analyzed by an expert in iCys-cell cytometry.
In parallel, practical training on advanced Fluorescence microscope, – data acquisition and analysis
of cell samples brought by individual participants, under guidance of Dr. Cieślar-Pobuda.
Please send email to Dr. Los (marek.los@liu.se) for booking time on the iCys instrument with Dr.
Grabarek.
Should the participants be interested in additional times on the iCys instrument or on the advanced
fluorescence microscope, please contact the operators of the equipment directly, during the course.
17.00 – 17.30 Forum discussion including tutors and interested participants.
Day II, Tuesday: 28.07.2015
9.00 –10.00 Demonstration of the operation and capabilities of Gallios flow cytometer under
guidance of Mayur V. Jain.
10.00 – 12.00 Practical training on iCys for selected participants – data acquisition and analysis of
cell samples brought by individual participants. Course-participants will have the opportunity to
have their sample analyzed by an expert in iCys-cell cytometry.
In parallel, practical training on advanced fluorescence microscope, – data acquisition and analysis
of cell samples brought by individual participants, under guidance of Dr. Cieślar-Pobuda.
12.00 – 13.00 lunch break
LOCATION: Avsikten, (close to entrance 65).
13.00 – 13.50 “Detection and quantification of membrane-associated signaling processes using TIRF
technology”. (E. Wiechec)
13.50 – 14.00 coffee break
14.00 – 14.50 “Detection of low-number cell populations – other methods” (Marek Los)
15.00 – 17.00 Practical training on iCys for selected participants – data acquisition and analysis of
cell samples brought by individual participants. Course-participants will have the opportunity to
have their sample analyzed by an expert in iCys-cell cytometry.
In parallel, practical training on advanced fluorescence microscope, – data acquisition and analysis
of cell samples brought by individual participants, under guidance of Dr. Cieślar-Pobuda.
Day III - IV, Wednesday - Thursday: 29-30.07.2015
Thursday, 30.07.2015, LOCATION: “Avsikten” (close to entrance 65).
10.00 – 10.30, “Flow Cytometry – recapitulation” (Dr Artur Cieslar-Pobuda)
10.30 – 11.15, “Flow cytometry-based diagnosis of Leukemias” (Lecture, Dr Mai Prokowska)
11.15 – 11.30 break
11.30 – 12.15, “Flow cytometry-based diagnosis of Lymphomas” (Lecture: Dr Mai Prokowska)
12.15 – 13.00 Lunch-break
13.00 – 16.00 Seminar: Diagnosis of Leukemias & Lymphomas, Practical exercises based on
clinical samples (Dr Mai Prokowska).
Day V, Friday: 31.07.2015
9.00 – 16.00 Practical training on iCys, – data acquisition and analysis of cell samples brought by
individual participants, under guidance of Dr. Grabarek.
In parallel, practical training on advanced Fluorescence microscope, – data acquisition and analysis
of cell samples brought by individual participants, under guidance of Dr. Cieślar-Pobuda.
17.00 Closing & Farewell ceremony with handing-out course attendance certificates, followed by
“get together” (french-fries, assorted pizzas, kebab, other southern dishes, and drinks will be
available).
For those who want to leave earlier (or for local participants), course attendance certificates could
be obtained earlier, as long as participant received an individual practical training on at least one
of the instruments.