UNIVERSITY OF WESTMINSTER
School of Biosciences
Control of Substances Hazardous to Health (COSHH)
***** APPENDIX 4 - CHEMICAL SAFETY *****
Sheet........... of............
Title of Experiment
Room/Laboratory
Site
SDS / PAGE and protein staining
5.08
NCS
1.
Brief description of work
Sodium dodecyl sulphate poly acrylamide gel electrophoresis. Silver / Coomassie Blue staining of proteins.
Note that a COSHH assessment for class activity may not be of a sufficiently high level to cover technician preparation; double assessment usually
required. Note also that it is RISK that is to be assessed, not HAZARD.
2.
LIST OF SUBSTANCES USED OR CREATED (continue on a separate sheet if necessary)
NAME OF SUBSTANCE
(CODE)
PHYSICAL
DESCRIPTION
Tris base
Solid
MAX.
QUANTITY
EXPOSED
TO
G
DATA
AVAILABLE
(YES/NO)
Sodium dodecyl sulphate
Solid
G
Y
Acrylamide Bis
Liquid
Ml
Y
Glycine
Solid
G
Y
Laemmli sample buffer
Liquid
Ml
y
Hydrochloric acid
Liquid
Ml
Y
Ammonium persulphate
Solid
mg
y
Temed N N N’N’tetramethylethylenediamine electran
Liquid
l
y
Methanol
Liquid
Ml
Y
Acetic acid (glacial)
(vinegar)
Liquid
Ml
Y
Amino caproic acid
Solid
G
Y
Silver nitrate
Solid
G
Y
37% Formaldehyde solution
Liquid
l
Y
Sodium thiosulphate
Solid
g
Y
Coomassie Blue
Solid
g
y
Y
KNOWN HAZARDS
(See Note;
use numbers)
7 (irritant) hygroscopic
15 (skin or eye)
5 (harmful if swallowed)
7 (irritant)
15 (skin or eye)
8 (possible carcinogen)
4 (toxic)
5 (harmful),
15 (skin or eye)
Not fully known but
avoid contact and
inhalation.
7 (irritant)
4 (toxic)
15 (skin or eye)
4 (toxic)
7 (irritant)
15 (skin or eye)
5 (harmful)
7 (irritant)
15 (skin or eye)
3 (highly flammable)
5 (harmful)
6 (corrosive)
15 (skin or eye)
3 (flammable), 4 (toxic),
7 (irritant),
15 (skin and eyes)
3 (flammable)
5 (harmful)
6 (corrosive)
5 (harmful)
7 (irritant)
15 (skin or eyes)
2 (oxidising),
6 (corrosive)
5 (harmful)
8 (limited evidence of
carcinogenic effect)
4 (toxic)
5 (harmful)
15 (skin or eye)
8 (potential cancer
hazard)
7 (irritant)
15 (skin or eyes)
Not known but avoid
contact and inhalation.
NOTE: 1) EXPLOSIVE
2) OXIDIZING 3) FLAMMABLE 4) TOXIC 5) HARMFUL 6) CORROSIVE 7) IRRITANT 8) CARCINOGEN 9) MUTAGEN 10)
TERATOGEN 11) DUST 12) INHALATION 13) INGESTION 14) SKIN ABSORPTION 15) SKIN OR EYE 16) INJECTION 17) MAXIMUM EXPOSURE LIMIT 18)
OCCUPATIONAL EXPOSURE STANDARD 19) RADIATION 20) OTHER (specify)
Ref Note 17, 18: see EH40 (current edition).
3.
PERSONNEL INVOLVED WITH SUBSTANCES (continue on a separate sheet if necessary)
NAME/CLASS
INITS
STATUS
Dwek
MV
Supervisor /
Lecturer
Markiv
A
Student
4.
INVOLVEMENT - DATE OF USE or
DAILY/WEEKLY/MONTHLY/OCCASIONALLY
Occasionally
Daily then occasionally
OTHER GROUPS/PERSONS WHO MAY HAVE ACCESS TO THE SUBSTANCES
(e.g. students, cleaners, maintenance staff, contractors, visitors, storekeepers, etc.):
Stores
State control measures (to prevent inappropriate persons/groups having access to the substances ):
Store in locked cabinet with other corrosive / toxic materials. Access to named personnel only.
5.
Cabinet should be well ventilated.
EMERGENCY PROCEDURES (as per Hazard Data Sheet).
if any of the substances or procedures identified above is likely to pose a special hazard in an emergency, then identify below action to be taken.
CODE
Tris base
Sodium dodecyl sulphate
Glycine
Hydrochloric acid
Acrylamide Bis
Ammonium persulphate
Temed N N N’N’tetramethylethylenediamine
electran
Methanol
Acetic acid
Amino caproic acid
Silver nitrate
Formalin solution
Sodium thiosulphate
Coomassie Blue
Laemmli sample buffer
SPILLAGE/UNCONTROLLED RELEASE
Wear respirator, chemical safety goggles, rubber
boots and heavy rubber gloves. Sweep up, place in
bag and hold for waste disposal Avoid raising dust
and ventilate area and wash spill site after material
pick-up is complete.
Ditto
Ditto
Wear respirator, chemical safety goggles, rubber
boots and heavy rubber gloves. Absorb on sand
and place in closed containers for disposal.
Ventilate area and wash spill site after material
pick-up is complete.
Ditto
Wear respirator, chemical safety goggles, rubber
boots and heavy rubber gloves. Sweep up, place in
bag and hold for waste disposal Avoid raising dust
and ventilate area and wash spill site after material
pick-up is complete.
Wear respirator, chemical safety goggles, rubber
boots and heavy rubber gloves. Absorb on sand
and place in closed containers for disposal.
Ventilate area and wash spill site after material
pick-up is complete.
Wear respirator, chemical safety goggles, rubber
boots and heavy rubber gloves. Absorb on sand
and place in closed containers for disposal.
Ventilate area and wash spill site after material
pick-up is complete.
Ditto
FIRE
Use a water spray, carbon dioxide or dry chemical
powder / foam. Emits toxic fumes under fire
conditions
Wear respirator, chemical safety goggles, rubber
boots and heavy rubber gloves. Sweep up, place in
bag and hold for waste disposal Avoid raising dust
and ventilate area and wash spill site after material
pick-up is complete.
Ditto
Ditto
Wear respirator, chemical safety goggles, rubber
boots and heavy rubber gloves. Absorb on sand
and place in closed containers for disposal.
Ventilate area and wash spill site after material
pick-up is complete.
Wear respirator, chemical safety goggles, rubber
boots and heavy rubber gloves. Sweep up, place in
bag and hold for waste disposal Avoid raising dust
and ventilate area and wash spill site after material
pick-up is complete.
Ditto
Ditto
Wear respirator, chemical safety goggles, rubber
boots and heavy rubber gloves. Cover with
activated carbon adsorbent and place in closed
containers for disposal. Ventilate area and wash
spill site after material pick-up is complete.
Ditto
Ditto
Ditto
Ditto
Ditto
Ditto
Use a water spray, carbon dioxide or dry chemical
powder / foam. Emits toxic fumes under fire
conditions. Formation of explosive mixtures
possible with air.
Use a water spray, carbon dioxide or dry chemical
powder / foam. Emits toxic fumes under fire
conditions.
Vapour may travel considerable distance to source
of ignition and flash back.
Use a water spray, carbon dioxide or dry chemical
powder / foam. Emits toxic fumes under fire
conditions.
Ditto
Non combustible, use extinguishing media
appropriate for the surroundings. Emits toxic fumes
under fire conditions.
Use a water spray, carbon dioxide or dry chemical
powder / foam. Emits toxic fumes under fire
conditions.
If personnel are affected (fumes, contamination, etc.) treatment to be adopted:
SPECIFY PER SUBSTANCE
(CODE AS PER HAZARD DATA SHEET)
If swallowed, wash out mouth with water provided if person is conscious. Call a physician.
If inhaled remove to fresh air. If breathing becomes difficult call a physican. In case of contact immediately wash skin with soap and copious quantities of water. In
case of contact with eyes, flush with copious quantities of water for at least 15 minutes. Assure adequate flushing by separating eyelids with fingers. Call a
physician.
Acrylamide BIS / Silver Nitrate
In case of an accident or if you feel unwell, seek medical advice immediately. In case of contact with eyes rinse immediately with plenty of water and seek
medical advice.
(NB. Antidotes and special treatment may be obtained through...................................................................................................................................)
6.
CONTROL MEASURES TO BE ADOPTED (NB. Consider the following points, the appropriate Biosciences Safety Code plus any other
necessary measures. SPECIFY PER SUBSTANCE AS NECESSARY FOR EACH OF THE ITEMS BELOW.)
Always use acids, solvents, silver and formalin solutions in fume cupboard, wear gloves (see COSHH form attached). Always add acid to water in small volumes NOT
water to acid.
1. Limiting exposure time of personnel - state maximum time
2. Partial enclosure with local exhaust ventilation
3. Local exhaust ventilation
4. Sufficient general ventilation
5. Personal protective equipment (goggles/gloves/clothing/mask/breathing apparatus/safety screen, etc.) SPECIFY
* 6. Safe disposal/storage of hazardous substances
* 7. Regular cleaning of the workplace
* 8. Adequate washing facilities
* = MANDATORY
STORAGE - SAFETY CONSIDERATIONS
HANDLING PRECAUTIONS
glacial acetic acid and HCl – toxic store cabinet
Methanol – solvent store
Acrylamide BIS – shelf of lab.
Temed - fridge
Always wear gloves and goggles.
Use in fume hood.
Salts and buffers lab shelf.
Wear gloves and avoid breathing dust
Take care when running the electrophoresis due to the high
voltages. Always turn off the power before disconnecting
the tanks.
DISPOSAL PROCEDURES DURING AND AT END OF EXPERIMENT
Dispose of plasticware after autoclaving via laboratory waste route. Dispose acid solutions via chemical disposal officer. Dispose of toxic waste and solvents via
chemical disposal officer.
Ensure that the silver solution is not put down the sink – EXPLOSION RISK – dispose into beaker, add some HCl
and allow to form silver chloride salts by precipitation. Dispose of salts via chemical disposal officer.
7.
REVIEW AND MONITORING OF CONTROL MEASURES
(Required checks, and their frequency, on the adequacy and maintenance of the control measures during the course of the experiment)
(NB. Biosciences Safety Code in respect of health surveillance needs. This form is not adequate if health surveillance is required.)
8.
OTHER RELEVANT INFORMATION (continue on a separate sheet if necessary)
(e.g. restriction of usage; transportation risks; labelling requirements; assessment review date, etc.)
9.
I certify that, to the best of my knowledge, the above precautions will adequately control the risks from the hazardous substances listed. I have brought the
assessment to the notice of the relevant technicians; academic staff; students; researchers (delete as necessary).
Name of Assessor:
Miriam Dwek
Signed:
Status of Assessor:
Lecturer
Date: