Name: ________________________________________________ Date: _______________ Period: ______
Amgen Pre-lab Quiz (version A)
1. Plasmids can be found in:
a. Protozoans
b. Prokaryotes
c. Fungi
d. Animals
e. Plants
2. Ampicillin and kanamycin are:
a. Genes
c. Restriction enzymes
b. Plasmids
d. Antibiotics
e. Bacteria
3. Plasmids are cut at precise locations by:
a. Restriction enzymes
c. Bacteria
b. Antibiotics
d. Sticky ends
e. Selectable markers
4. Gel electrophoresis is a technique used to:
a. Break plasmids into small fragments
b. Connect sticky ends of two plasmids
c. Separate small pieces of DNA according to their size
d. Check to see if bacteria are resistant to antibiotics
5. Below is a circular plasmid that is 4000 base pairs (bp) of DNA in size.
Chemicals have been engineered by scientists to cut the plasmid at
precise locations. Chemical #1 will cut the plasmid at the 150th base pair.
Chemical #2 will cut the plasmid at the 2,200th base pair. After the
plasmid has been cut by these two chemicals, how many plasmid
fragments will be created?
Chemical #2
cuts here
Chemical #1
cuts here
4000 bp
6. How many base pairs is the largest and the smallest fragment?
7. Because the plasmids are so expensive…
a. You will be using artificially plasmids in a simulation
b. The same plasmid is share by many high schools in the Amgen program
c. They are counted in kilograms
d. You will be given a very small amount
8. To avoid cross-contamination when using a micropipette, you should always…
a. Wash your hands before using the micropipette
b. Change the tip of the micropipette
c. Run the electrophoresis gel using the supplied plasmids
d. Use two hands when holding the micropipette
9. A microtube contains 0.00001L of water, .007ml of pKAN, and 5µl of buffer. How many total
microliters are in the microtube?
Name: ________________________________________________ Date: _______________ Period: ______
Amgen Pre-lab Quiz (version A)
1. Plasmids can be found in:
a. Protozoans
b. Prokaryotes
c. Fungi
d. Animals
e. Plants
2. Ampicillin and kanamycin are:
a. Genes
c. Restriction enzymes
b. Plasmids
d. Antibiotics
e. Bacteria
3. Plasmids are cut at precise locations by:
a. Restriction enzymes
c. Bacteria
b. Antibiotics
d. Sticky ends
e. Selectable markers
4. Gel electrophoresis is a technique used to:
a. Break plasmids into small fragments
b. Connect sticky ends of two plasmids
c. Separate small pieces of DNA according to their size
d. Check to see if bacteria are resistant to antibiotics
5. Below is a circular plasmid that is 4000 base pairs (bp) of DNA in size.
Chemicals have been engineered by scientists to cut the plasmid at
precise locations. Chemical #1 will cut the plasmid at the 150th base pair.
Chemical #2 will cut the plasmid at the 2,200th base pair. After the
plasmid has been cut by these two chemicals, how many plasmid
fragments will be created?
Chemical #2
cuts here
Chemical #1
cuts here
4000 bp
6. How many base pairs is the largest and the smallest fragment?
7. Because the plasmids are so expensive…
a. You will be using artificially plasmids in a simulation
b. The same plasmid is share by many high schools in the Amgen program
c. They are counted in kilograms
d. You will be given a very small amount
8. To avoid cross-contamination when using a micropipette, you should always…
a. Wash your hands before using the micropipette
b. Change the tip of the micropipette
c. Run the electrophoresis gel using the supplied plasmids
d. Use two hands when holding the micropipette
9. A microtube contains 0.00001L of water, .007ml of pKAN, and 5µl of buffer. How many total
microliters are in the microtube?