Michael A. Ewing
(last updated: July 3, 2015)
miewing@indiana.edu
3003 E Amy Lane
Bloomington, IN 47408
(317) 308-9680
Research Interests:
-Physical, analytical, and biological chemistry
-Applying gas-phase techniques to study populations of conformations of biological molecules
-Understanding the relationship between conformations across phase-transitions
-Understanding biomolecule motion in the absence of solvent
-Development of methods for rapid determination of chirality, especially at single sites within
large molecules containing multiple chiral centers
Education:
Ph.D. Analytical Chemistry, Expected August 2015
Indiana University, Bloomington, IN
Advisor: Professor David Clemmer
M.S. Analytical Chemistry, May 2008
University of Illinois at Urbana-Champaign, Urbana, IL
Thesis: “Novel Detection and Measurement Methods for D-Amino Acid-Containing Peptides”
Advisor: Professor Jonathan Sweedler
B.S. Chemistry, May 2003
B.S. Mathematics (Statistics Concentration), May 2003
Minor in Applied Biology
Minor in Electrical and Computer Engineering
Rose–Hulman Institute of Technology, Terre Haute, IN
Graduated magna cum laude
Professional Experience:
Indiana University, Bloomington, IN
Graduate Student
2010 – Present
 Developed high resolution ion mobility spectrometers
 Studied methods for predicting cross-section of biomolecules
 Examined energetics and kinetics of solution- and gas-phase structural transitions
Lancaster Laboratories, Indianapolis, IN
Chemist III
 Trace analysis of genotoxic impurities using GC-MS and LC-MS
 Contract work for a large pharmaceutical company
University of Illinois, Urbana-Champaign, IL
Graduate Student
 Studied neuropeptides of Aplysia californica
 Developed method to examine chirality of residues within neuropeptides
Rose-Hulman Institute of Technology, Terre Haute, IN
2008 – 2010
2003 – 2008
Resident Tutor
2000 – 2003
 Tutored math, science, and engineering subjects as part of the learning center
 Focused on sophomore classes but acted as lead tutor for a wide range of classes
Rose-Hulman Institute of Technology, Terre Haute, IN
Student Researcher
 Wrote a program for calculating energies of a double well potential
2000 – 2003
Rose-Hulman Institute of Technology, Terre Haute, IN
Teaching Assistant
2002 – 2003
 Graded homework and quizzes, supervised labs and worked on a curriculum in general
chemistry and advanced statistics courses
Indiana University, Bloomington, IN
REU Participant
 Developed a method to modify proteins to reduce charge state
 Processed and analyzed data for IMS-MS (IM-MS) experiments
University of Pittsburgh, Pittsburgh, PA
REU Participant
 Studied model potentials for molecular mechanics including water
Pennsylvania State University, University Park, PA
Research Assistant in Chemistry
 Constructed ultramicroelectrodes down to 110 nm in radius
 Wrote and troubleshot LabVIEW programs for instrument control
Honors:
ACS Division of Analytical Chemistry Graduate Research Fellowship
by Eastman Chemical Company
Department of Chemistry Teaching Award
National Science Foundation Graduate Research Fellowship
University of Illinois Fellowship
Department of Chemistry Teaching Award
William Albert Noyes Sr. Award in Chemistry
Clarence P. Sousley Award (Senior Math Award)
Putnam Commendation
Roger Louis Maness Memorial Scholarship (Chem Scholarship)
Theodore Paine Palmer Award (Freshman Math Award)
Presidential Scholarship (Rose-Hulman Scholarship)
Professional Memberships:
American Chemical Society
American Society for Mass Spectrometry
Summer 2002
Summer 2001
Summer 1998
Summer 2013
2011
2005 – 2007
2003 – 2005
2004
2003
2003
2003
2002
2000
1999 – 2003
Teaching Assignments:
Indiana University
CHEM C589/BIOC B541 Lecture – Enzyme Mechanisms
CHEM C588/BIOC B540 Lecture – Fundamentals of Biomolecular Catalysis
CHEM B486 Lecture – Gene Expression and Physiology
CHEM C582/BIOC B505 Lecture – Biomolecular Catalysis
CHEM C117 Discussion – Principles of Chemistry and Biochemistry I
CHEM C103 Discussion – Introduction to Chemical Principles
Spring 2015
Spring 2015
Spring 2012
Spring 2012
Spring 2011
Fall 2010
University of Illinois at Urbana-Champaign
Advanced Analytical Chemistry Laboratory and Lecture
Accelerated Chemistry Lab II
Accelerated Chemistry Lab I
Fall 2004
Spring 2004
Fall 2003
Rose-Hulman Institute of Technology
Laboratory – General Chemistry III
Laboratory – Organic Chemistry III
Lecture – Engineering Statistics II
Winter 2002-2003
Spring 2003
Fall 2002
Service:
2013 Gordon Research Conference on Biological Molecules in the Gas Phase & in Solution
Assistant to the chair
th
246 American Chemical Society National Meeting and Exposition
Fall 2013
ANYL symposium organizer, New Techniques and Methods in Proteomics – cancelled
Clemmer lab webmaster
2011 – Present
Peer Review Assignments:
Analytical Chemistry
2014 – Present
Outreach:
Foundations in Science and Mathematics, Chemistry Instructor
Summer 2013
http://www.iu.edu/~fsm/
Publications:
18. Ewing, M.A.; Glover, M.S.; Clemmer, D.E. Hybrid Ion Mobility and Mass Spectrometry as a
Separation Tool. Submitted.
17. Jung, J.E.; Ewing, M.A.; Valentine, S.J.; Przybylski, M.; Clemmer, D.E. Structural Insights Into
Linkage-Specific Ubiquitin Chains Using Ion Mobility-Mass Spectrometry. Submitted.
16. Shi, L.; Holliday, A.E.; Glover, M.S.; Ewing, M.A.; Russell, D.H.; Clemmer, D.E. A Balance of
Energy and Entropy Across Multiple Intermediates Enables Solution-Induced Folding of Polyproline
Helices. Submitted.
15. Larriba-Andaluz, C.; Fernandez-Garcia, J.; Ewing, M.A.; Hogan, C.; Clemmer, D.E. Gas Molecule
Scattering & Ion Mobility Measurements for Organic Macro-ions in He versus N2 Environments.
Phys. Chem. Chem. Phys. 2015, 17, 15019-15029.
14. Ewing, M.A.; Conant, C.R.P.; Zucker, S.M.; Griffith, K. J.; Clemmer, D.E. Selected Overtone
Mobility Spectrometry. Anal. Chem. 2015, 87, 5132-5138.
13. Shi, L.; Holliday, A.E; Shi, H.; Zhu, F.; Ewing, M.A.; Russell, D.H.; Clemmer, D.E. Characterizing
Intermediates along the Transition from Polyproline I to Polyproline II using Ion Mobility
Spectrometry-Mass Spectrometry, J. Am. Chem. Soc. 2014, 136, 12702-12711.
12. Zucker, S.M.; Ewing, M.A.; Clemmer, D.E. A Gridless Ion Elimination Mechanism for Attomole
Detection with Overtone Mobility Spectrometry, Anal. Chem. 2013, 85, 10174-10179.
11. Glaskin, R.S.; Ewing, M.A.; Clemmer, D.E. Ion Trapping for Improving Ion Mobility Spectrometry
Measurements in a Cyclical Drift Tube, Anal. Chem. 2013, 85, 7003-7008. Editors Highlight.
10. Ewing, M.A.; Zucker, S.M.; Valentine, S.J.; Clemmer, D.E. Overtone Mobility Spectrometry: Part 5.
Simulations and Analytical Expressions Describing Overtone Limits. J. Am. Soc. Mass Spectrom.
2013, 24, 768-779.
9. Dilger, J.M.; Valentine, S.J.; Glover, M.S.; Ewing, M.A.; Clemmer, D.E. A Database of Alkali MetalContaining Peptide Cross-Sections: Influence of Metals on Size Parameters for Specific Amino Acids.
Int. J. Mass Spectrom. 2012, 330-332, 35-45.
8. Valentine, S.J.; Ewing, M.A.; Dilger, J.M.; Glover, M.S.; Geromanos, S.J.; Hughes, C.; Clemmer, D.E.
Using Ion Mobility Data to Improve Peptide Identification: Intrinsic Amino Acid Size Parameters. J.
Proteome Res. 2011, 10, 2318-2329.
7. Lee, S.; Ewing, M.A.; Nachtigall, F.M.; Kurulugama, R.T.; Valentine, S.J.; Clemmer, D.E..
Determination of cross sections by overtone mobility spectrometry: evidence for loss of unstable
structures at higher overtones. J. Phys. Chem. B 2010, 114, 12406-12415.
6. Ewing, M.A.; Wang, J.; Sheeley, S.A.; Sweedler, J.V.. Detecting D-amino acid-containing peptides
using selective enzymatic digestion. Anal. Chem. 2008, 80, 2874-2880.
5. Hummon, A.B.; Richmond, T.A.; Verleyen, P.; Baggerman, G.; Huybrechts, J.; Ewing, M.A.;
Vierstraete, E.; Rodriguez-Zas, S.L.; Schoofs, L.; Robinson, G.E.; Sweedler, J.V. From the genome to
the proteome: uncovering peptides in the Apis brain. Science 2006, 314, 647-649.
4. Honeybee Genome Sequencing Consortium. Insights into social insects from the genome of the
honeybee Apis melifera. Nature 2006, 443, 931-949.
3. Sheeley, S.A.; Miao, H.; Ewing, M.A.; Rubakhin, S.S.; Sweedler, J.V. Measuring D-amino acidcontaining neuropeptides with capillary electrophoresis. Analyst 2005, 130, 1198-1203.
2. Myung, S.; Lee, Y.J.; Moon, M.H.; Taraszka, J.; Sowell, R.; Koeniger, S.; Hilderbrand, A. E.;
Valentine, S. J.; Cherbas, L.; Cherbas, P.; Thomas, C.; Miller, D. F.; Mechref, Y.; Novotny, M. V.;
Ewing, M. A.; Sporleder, C. R.; Clemmer, D. E. Development of high-sensitivity ion trap ion mobility
spectrometry time-of-flight techniques: A high-throughput nano-LC-IMS-TOF separation of peptides
arising from a Drosophila protein extract. Anal. Chem. 2003, 75, 5137-5145.
1. Meyerhoff, J.B.; Ewing, M.A.; Ewing, A.G. Ultrasmall enzyme electrodes with response times less
than 100 milliseconds. Electroanalysis 1999, 11, 308-312.
Patents:
Clemmer, D.E.; Ewing, M.A. Hybrid ion mobility spectrometer e.g. single-pass ion mobility
spectrometer, for investigating properties of e.g. ions, has set of ion gates controlled between open
and closed positions to selectively pass ions into multiple-pass drift tube. WO2015048014-A1.
Clemmer, D.E.; Ewing, M.A.; Zucker, S.M.; Conant, C.R.P. Ion Mobility Spectrometer and Method of
Operating Same. US8872102-B2, US 20130292562 A1, US20150041637 A1.
Invited Talks:
Ewing, M.A.; Zucker, S.M.; Glover, M.S.; Clemmer. D.E. “Zoom Mode Ion Mobility Spectrometry.”
th
65 Pittsburgh Conference for Analytical Chemistry and Applied Spectroscopy, Chicago, IL, March 3,
2014.
Ewing, M.A.; Zucker, S.M.; Glover, M.S.; Clemmer. D.E. “Towards Zoom Mode Ion Mobility
Spectrometry.” Eastman Chemical Company, Kingsport, TN, October 21, 2013.
Oral Presentations:
Shi, L; Holliday, A.; Glover, M.; Ewing, M.; Russell, D.; Clemmer, D. “Ion Mobility-Mass
Spectrometry Reveals the Energy Landscape of Polyproline Folding.”63rd ASMS Conference on Mass
Spectrometry and Allied Topics, St. Louis, MO, June 3, 2015.
Clemmer, D.E.; Shi, L.; Pierson, N.; Shi, H.; Ewing, M. “Developing IMS-MS techniques as a means
of following structural transitions of biopolymers in solution.” 246th American Chemical Society National
Meeting and Exposition, Indianapolis, IN, September 11, 2013.
http://abstracts.acs.org/chem/246nm/program/divisionindex.php?act=presentations&val=Advances+i
n+Ion+Mobility+Spectrometry+and+FAIMS&ses=Advances+in+Ion+Mobility+Spectrometry+and+FAI
MS&prog=191000
Ewing, M.A.; Griffith, K.J.; Zucker, S.M.; Conant, C.R.P.; Clemmer, D.E. “Overtone mobility
spectrometry: Selection of overtones.” 246th American Chemical Society National Meeting and
Exposition, Indianapolis, IN, September 12, 2013.
http://abstracts.acs.org/chem/246nm/program/divisionindex.php?act=presentations&val=Developmen
ts+in+Mass+Spectrometry&ses=Developments+in+Mass+Spectrometry&prog=191000
Ewing, M.A.; Wang, J.; Sweedler, J.V. “Using mAAP to Search for D-Amino Acid-Containing
Peptides in the Aplysia californica Brain.” 57th Pittsburgh Conference for Analytical Chemistry and
Applied Spectroscopy, Orlando, FL, March 12 – 17, 2006.
Ewing, M.A.; Wang, J.; Sweedler, J.V. “A new method for finding D-amino acid-containing
Peptides.” Turkey Run 2005 Analytical Chemistry Conference, Marshall, IN, November 5, 2005.
Sweedler, J.; Miao, H.; Ewing, M.; Scanlan, C.; Rubakhin, S. “Chirality in your Brain: Detecting DAmino Acids and D-Amino Acid-Containing Peptides in Neurons.” ACS National Meeting, Washington,
DC, August 2005.
Ewing, M.A.; Wang, J. Sweedler, J.V. “Detecting D-Amino Acid-Containing Peptides.” 56th
Pittsburgh Conference for Analytical Chemistry and Applied Spectroscopy, Orlando, FL, Feb 27 – March
4, 2005.
Ewing, M.A.; Wang, J.; Sweedler, J.V. “Detecting D-amino acid-containing neuropeptides in the
CNS of Aplysia californica.” 36th ACS Great Lakes Regional Meeting, Peoria, IL, Oct 18, 2004.
http://acs.confex.com/acs/36glrm/techprogram/P9656.HTM
Ewing, M.A.; Sheeley, S.A.; Sweedler, J.V. “Detecting D-Amino Acid-Containing Peptides.” 55th
Pittsburgh Conference for Analytical Chemistry and Applied Spectroscopy, Chicago, IL, March 8 – 12,
2004.
Ewing, M.; Jelski, D. “A Model for Displacive Anharmonic Vibrational Motions.” Butler University
14th Annual Undergraduate Research Conference, Indianapolis, IN, April 12, 2002.
Ewing, M.; Nei, P.; Schneider, T. “Rookies in the Mathematical Modeling Contest.” 17th Annual
Rose-Hulman Undergraduate Math Conference, Terre Haute, IN, March 18, 2000.
http://www.rose-hulman.edu/mathconf/2000/UndergradConf00.html
Poster Presentations:
Ewing, M.; Glover, M.; Zucker, S.; Clemmer, D. “Zoom Mode Ion Mobility Spectrometry.” 63rd
ASMS Conference on Mass Spectrometry and Allied Topics, St. Louis, MO, June 1, 2015.
Ewing, M. A.; Zucker, S. M.; Glover, M. S.; Clemmer, D. E. “Zoom Mode Ion Mobility
Spectrometry.” NOBCChE Research Symposium, Bloomington, IN, September 27, 2014.
Zucker, S.M.; Ewing, M.A.; Clemmer, D.E. “Gridless Overtone Mobility Spectrometry.” Biological
Molecules in the Gas Phase & in Solution Gordon Research Conference, Holderness, NH, July 24, 2013.
Ewing, M.A.; Conant, C.R.P.; Zucker, S.M.; Griffith, K.J.; Clemmer, D.E. “Overtone Mobility
Spectrometry for Examining Structural Transitions in the Gas-Phase.” Biological Molecules in the Gas
Phase & in Solution Gordon Research Conference, Holderness, NH, July 22, 2013.
Ewing, M.A.; Zucker, S.M.; Valentine, S.J.; Clemmer, D.E.. “Insights into Overtone Mobility
Spectrometry.” Turkey Run 2012 Analytical Chemistry Conference, Marshall, IN, Nov 2, 2012.
Conant, C.R.P.; Ewing, M.A.; Clemmer, D.E. “Selected Overtone Mobility Spectrometry: Expanding
the Range of Overtone Mobility Spectrometry.” 2012 Turkey Run Analytical Chemistry Conference,
Marshall, IN, Nov 2, 2012
Zucker, S.M.; Ewing, M.A.; Glaskin, R.S.; Valentine, S.J.; Clemmer, D.E. “Gridless Overtone
Mobility Spectrometry with Continuous RF Confinement.” Turkey Run 2012 Analytical Chemistry
Conference, Marshall, IN, Nov 2, 2012
Ewing, M.A.; Valentine, S.J.; Dilger, J.M.; Glover, M.S.; Geromanos, S.; Hughes, C.; Clemmer, D.E.
“The Use of a Synapt-Generated Cross Section Database and Intrinsic Amino Acid Size Parameters for
the Improvement of Peptide Identification.” 59th Annual ASMS Conference on Mass Spectrometry and
Allied Topics, Denver, CO, June 7, 2011.
Ewing, M.A.; Wang, J.; Sweedler, J.V. “Detecting D-Amino Acid-Containing Neuropeptides in the
CNS of Aplysia californica.” 53rd ASMS Conference on Mass Spectrometry, San Antonio, TX, June 5 –
9, 2005.
Ewing, M.A.; Jordan, K. D. “A Model Potential for Water.” Duquesne 2001 Summer Research
Symposium, Pittsburgh, PA, July 9, 2001