H
M.
J
University of California, Berkeley, Chemistry Department
Lawrence Berkeley National Laboratory, Molecular Foundry
1 Cyclotron Road, MS 67R5110

Berkeley, CA 94702

510-219-4169

HMJensen@lbl.gov
E D U C A T I O N :
P H . D.
C A N D I D A T E , E X P E C T E D G R A D U A T I O N D A T E D E C ,2012
U N I V E R S I T Y O F C A L I F O R N I A , B E R K E L E Y
T H E S I S A D V I S O R S : J A Y T .
G R O V E S & C A R O L I N E M.
A J O -F R A N K L I N
B.S.
B I O C H E M I S T R Y , J U N E 2 007
C A L I F O R N I A P O L Y T E C H N I C S T A T E U N I V E R S I T Y , S A N L U I S O B I S P O
T H E S I S A D V I S O R : D R .
C H A D I M M O O S
P R O F E S S I O N A L E X P E R I E N C E :
G R A D U A T E S T U D E N T R E S E A R C H E R : Aug 2007 – present
Jay T. Groves – University of California, Berkeley
Caroline M. Ajo-Franklin – Lawrence Berkeley National Lab
Engineering an extracellular electron conduit in live microbes to enable electronic communication with inorganic materials.

Heterologous expression of mtrCAB from Shewanella oneidensis MR-1 in E. coli , the first time two multiheme c-cytochromes were expressed simultaneously. Additionally, we have now optimized expression to include a 3 rd cytochrome, cymA .

Demonstrated functional expression of the mtrCAB genes in E. coli by reduction of soluble Fe(III) citrate and insoluble Fe
2
O
3
.

Investigated the interaction between native E. coli redox-active proteins and heterologously expressed MtrA.

Elucidated correlation between Mtr and Ccm expression with cell health and electron transfer capability

Designed electrochemical cell and measured current production from engineered E. coli strains.
G R A D U A T E S T U D E N T I N S T R U C T O R : Fall Semesters of 2007, 2008, 2009
Chem 1A, MCB 110L, Chem 4A – University of California, Berkeley
Teaching introductory chemistry and molecular biology to undergraduates.
U N D E R G R A D U A T E R E S E AR C H A S S I S T A N T , June – August 2006
Ursula Gibson – Dartmouth College, Center of Nanotechnology
Construction and testing of solar dye-sensitized Graetzel cells with the ZnO nanowires stained with purified organic dyes from raspberries.
U N D E R G R A D U A T E R E S E AR C H A S S I S T A N T , June 2005 – June 2007
Chad Immoos – Cal Poly SLO, Department of Chemistry
Two posters presented at the 2006 National ACS Convention in San Francisco.
Construction of multilayer nanoparticle-embedded polyelectrolyte films for testing chemical warfare agent degradation.
L A B O R A T O R Y & M A N A G E M E N T S K I L L S :
Expert in molecular biology and cloning techniques
General biochemistry techniques, such as western blotting, cell fractionation, protein isolation, etc.

Protein engineering of complex membrane electron transfer systems
Optimizing expression of multiple membrane proteins with complex post-translational modifications
Development of in vivo kinetic techniques
Self-motivated toward development of novel techniques in a new lab
Nanoscale interactions of microbial communities and inorganic materials
Designing electrochemical systems for measuring bacterial current
Microscopy, such as DIC and fluorescence microscopy
Self-deployed organization and management of the lab’s 500+ strains.
Supervising and mentoring experience with 6 undergraduate summer students and new graduate student
A W A R D S & H O N O R S :
One of only 15 abstracts chosen from 150 submitted to Synthetic Biology 5.0 (SB5.0), June 2011
Best of BIOT Webinars, Selected from the “Emerging Technologies: Synthetic Biology” Section at the 2010 Spring ACS Meeting
People’s Choice Award for the poster “Electrifying
E. coli : Splicing Electron Transfer
Pathways”, Berkeley Nanotechnology Forum, April 2010
3 rd place in Bears Breaking Boundaries Competition in the Energy and Environmental
Innovations Section for “Plugging in Bacteria: Directly harnessing electrical current from photosynthetic microbes.”
Frost Undergraduate Research Fellowship, June 2005
P U B L I C A T I O N S :
H. M. Jensen , J. T. Groves, and C. M. Ajo-Franklin. Factors affecting metal reducting function in a complete electron conduit in Escherichia coli. . In preparation.
H. M. Jensen , M. Kokish. T. Groves, and C. M. Ajo-Franklin. Interprotein electron transfer is a critical bottleneck in metal reduction of engineered E. coli . In preparation.
C. Goldbeck, H. M. Jensen , M. A. TerAvest, N. Beedle, Y. Appling, M. Hepler, G. Cambray, V.
Mutalik, L.T. Angenent, and C. M. Ajo-Franklin. (2013) Tuning promoter strengths for improved synthesis and function of electron conduits in Escherichia coli . ACS Synthetic Biology , Accepted.
H. M. Jensen , A. E. Albers, K. Malley, Y. Y. Londer, B. E. Cohen, B. A. Helms, P. Wiegele, J. T.
Groves, and C. M. Ajo-Franklin. (2010) Engineering of a synthetic electron conduit in living cells.
PNAS. 107: 19213-18.
I N V I T E D T A L K S :
“Electrifying Bacteria: Growing electrical connections in living cells,” Heather M. Jensen . “Current
Research in Chemistry” (Chem85) guest speaker at City College of San Francisco, Feb 14, 2012,
San Francisco, CA, USA.
“Electrifying E. coli : Splicing Electron Transfer Pathways,” Heather M. Jensen , Kostia Malley, Jay T.
Groves, Caroline M. Ajo-Franklin. Best of BIOT Webinars, Selected from the “Emerging
Technologies: Synthetic Biology” Section at the 2010 Spring ACS Meeting. Jan 19, 2011. https://acsbiot.webex.com/
T
A L K S
:
“Electrifying Bacteria: Growing and Optimizing Electrical Conduits in Living Cells” Heather M.
Jensen , Jay T. Groves, Caroline M. Ajo-Franklin. The Molecular Foundry - NCEM Users
Meeting, Oct 4-5, 2012, Berkeley, CA, USA.
“Building Electrical Conduits in Living Cells,” Heather M. Jensen , Jay T. Groves, Caroline M. Ajo-
Franklin. American Chemical Society Spring Meeting, Mar 24-29, 2012, San Diego, CA, USA.

“Molecularly Defined Re-wiring of Electron Transport in Living Cells,”
Heather M. Jensen , Jay T.
Groves, Caroline M. Ajo-Franklin. Biophysical Society Spring Meeting, Feb 25-29, 2012, San
Diego, CA, USA.
“Engineered Electrical Conduits in Living Cells,” Heather M. Jensen , Jay T. Groves, Caroline M. Ajo-
Franklin. SB5.0, June 15-17, 2011, Standford, CA, USA.
One of only 15 abstracts chosen from 150 submitted.
“Engineering Electrical Connections in Living Cells,” Heather M. Jensen , Jay T. Groves, Caroline M.
Ajo-Franklin. Materials Research Society Spring Meeting, Apr 25-29, 2011, San Francisco, CA,
USA.
“Growing Electrical Connections in Living Cells,” Heather M. Jensen , Jay T. Groves, Caroline M. Ajo-
Franklin. Biophysical Society Spring Meeting, Mar 5-9, 2011, Baltimore, CA, USA.
"Forming Direct Electrical Connections Between Microbes and Electrodes," Heather M. Jensen , Jay T.
Groves, Caroline M. Ajo-Franklin, The Molecular Foundry - NCEM Users Meeting, Sept 31-Oct
1, 2010, Berkeley, CA, USA.
"Electrifying E. coli : Splicing Electron Transfer Pathways," Heather M. Jensen , Kostia Malley, Jay T.
Groves, Caroline M. Ajo-Franklin. American Chemical Society Meeting, March 21-25, 2010, San
Francisco, CA, USA.
P O S T E R S :
“Electrifying E. coli : Splicing Electron Transfer Pathways” Poster. Heather M. Jensen , Jay T. Groves,
Caroline M. Ajo-Franklin. Berkeley Nanotechnology Forum, April 25, 2010, Berkeley, CA,
USA.
"Wiring up Bacteria: Making Electrical Contacts with E. coli ," Heather M. Jensen , Jay T. Groves,
Caroline M. Ajo-Franklin. Synthetic Biology 4.0, 10-12 October 2008, Clear Water Bay,
Kowloon, Hong Kong.
P R O F E S S I O N A L A F F I L I A T I O N S :
Science, Technology, and Engineering Policy Group, UC Berkeley
Alpha Chi Sigma, Professional Chemistry Fraternity
American Chemical Society
Biophysical Society
Materials Research Society
Ambassador for the College of Science and Mathematics, Cal Poly SLO
Golden Key Association