HYDROPHILIC MODIFICATION of Au NANOPARTICLE on QCM
SURFACE by
PLASMA DEPOSITION for BIOMEDICAL APPLICATION
Ko-Shao Chen1, Shu-Juan Liao1 ,Tsui-Hsun Wu2 , Yi-You Huang2 ,Hsin-Ming Wu 1,
Yi-Shiuan Wei1
1 Department
2 Institute
of Materials Engineering, Tatung University, Taipei, Taiwan
of Biomedical Engineering, National Taiwan University, Taipei, Taiwan
E-mail kschen@ttu.edu.tw
Abstract
Most studies on nanoparticle metallic material are gold nanoparticle because of its
unique optical property, simple preparation, and great stability. Since the combination
of gold nanoparticles (AuNPs) with polishing quartz crystal microbalance (QCM) is
difficult for biosensor application, the surface properties of the QCM must be
modified prior to its combination with AuNPs. In this study, gold nanoparticles were
prepared by the reduction method by using trisodium citrate combined with hydrogen
tetrachloroaurate ( ) tetrahydrate (chloroauric acid), and modified with
11-Mercaptoundecanoic acid (MUA) by the self-assembly monolayer. The polishing
quartz crystal microbalance (QCM) electrodes surface was first subjected to plasma
deposition of Hexamethyldisilazane (PD-HMDSZ) and followed by O2 plasma
treatment. The content of AuNPs was determined by the relation between frequency
shift of modified QCM and concentration of AuNPs. This study shows that after
HMDSZ deposition treatment the QCM surface become hydrophobic, and after both
HMDSZ and O2 treatments the water contact angle of the QCM surface decreases to
less than 10° and exhibits hydrophilic property. The plasma surface treatments by
both HMDSZ and O2 on the QCM surface obviously improve the sensitivity of
frequency measurement of AuNPs. The AuNPs on the modified electrode surface
proposed in this study offer a new strategy of immoblized biomolecules for
electrochemical immunity sensor or indicator.
Keywords: AuNPs, plasma deposition, QCM, hydrophilic material, PD-HMDSZ
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