Online Resource 2.
Published microsatellite loci are listed below. Each locus had an additional M13 tag (CACGACGTTGTAAAACGAC) added to the 5’ end of the forward primer, with the
exception of Blm5, Blm10 and Blm12 that had the M13 tag added to the reverse primer. Those in bold font amplified successfully and consistently and were incorporated
into the genotyping system.
Locus
Primer sequence
References
GenBank accession numbers
Aph12
F: M13-TTAGTAGCATGTCAGGTTTATT
Maak et al 2000
AJ515888
R: GCTTGTAGACTTCAGAGTTC
Aph13
F: M13-CAACGAGTGACAATGATAAAA
Maak et al 2000
AJ515889
R: CAATGATCTCACTCCCAATAG
Aph15
F: M13-TGAATATGCGTGGCTGAA
Maak et al 2000
AJ515890
R: CAGTGAGGAATGTGTTTGAGTT
Aph16
F: M13-CCTTCTGAACCTTCGTAG
Maak et al 2000
AJ515891
R: AAATATAGACTTTTGTCCTGAA
Aph17
F: M13-GGACATTTTCAACCATAAACTC
Maak et al 2000
AJ515892
R: CATCCATGACAGACAGAAGA
Aph19
F: M13-CATGGAGCAAGCAATCGTCTG
Maak et al 2000
AJ515894
R: ACCACGTCCATCCTGAAGAAA
Aph20
F: M13-ACCAGCCTAGCAAGCACTGT
Maak et al 2000
AJ515895
R: GAGGCTTTAGGAGAGATTGAAAAA
Aph21
F: M13-CTTAAAGCAAAGCGCACGTC
Maak et al 2000
AJ515896
R: AGATGCCCAAAGTCTGTGCT
Aph23
F: M13-TCCTCTGCTCTAGTTGTGATGG
Maak et al 2000
AJ515898
R: CCTCAGCAGTCTTCCTCAGTG
Aph24
F: M13-TCAACCAGTGGTCAGAGAAAAA
Maak et al 2000
AJ515899
R: AGGTCAGCCCCCATTTTAGT
Aph25
F: M13-CCGTCAGACTGTAGGGAAGG
Maak et al 2000
AJ515900
R: AAAGCTCCACAGAGGCAAAG
Apl11
F: M13-AACTACAGGGCACCTTATTTCC
Denk et al 2004
AY498541
R: TTGCATCAGGGTCTGTATTTTC
Bcau4
F: M13-ACAACCTTCAAAGTCAATCCAAT
Buchholz et al 1998
AF025892
R: TCCTGACGCTCTCGGACGAGT
1
Bcau6
Bcau11
Blm5
Blm10
Blm12
Cam2
Cam3
Cam9
CmAAT
16
CM09
Sfiu2
Sfiu4
Smo7
JCC1
F: M13-TTTAACCCAGTAGCCTATCATGTCA
R: GTCTGAAGATAATGCTGCATGGTT
F: M13-TAGAAAAGGCTGAAGGAGTGGC
R: TGAGGAAGCAACTGTAAATAGGAGA
F: GCCACTTCTTTTGAAGTCACC
R: M13-GAAGCATCTTGTATGGCTTGC
F: CAAAGTATATCTTCTCAGGGACACG
R: M13-TGCATTGCTGTGAAGAGACC
F: TTCTGTGGGAGAAGACAAAGG
R: M13-ACTTGCCTGCTTCACTCC
F: M13-TCCACAAGGACACCATTAGG
R: GGTATTTCTTTTGC
F: M13-AACATCTACTTTGGCCTCTCC
R: TCTGTGCCCTGTTCTACTGC
F: M13-AATTGCAGCACTAATGAGC
R: GCTCATCAATCAAAACATTCC
F: M13-TCCCAAGGGTACCAGTGAA
R: TGTTGGCTCCCTGCTTAAAT
F: M13-GGATGTTGCCCCACATATTT
R: TTGCCTTGTTTATGAGCCAT
F: M13-ATAAACGGCTAATATGAAGTCT
R: AGGCTAGATATTGCTCTTATCCT
F: M13-TGAGGGGGAAGAGAATAAGAGA
R: CAGGGCAGTATTTTCAGGACATT
F: M13-TTTTCACCCAGTTCACTTCAGCC
R: GATTCAAATTTGCCGCAGGATTA
F: M13-GGATTGGAGATTTTCAGGAGC
R: AGGGAACTGATGCCCCA
Buchholz et al 1998
AF025894
Buchholz et al 1998
AF025899
Guay & Mulder 2005
AY766439
Guay & Mulder 2005
AY766443
Guay & Mulder 2005
AY766445
Carew et al 2003
AY130973
Carew et al 2003
AY130974
Carew et al 2003
AY130978
Stai and Hughes 2003
AF509882
Maak et al 2000
AJ271212
Fields & Scribner 1997
Fields & Scribner 1997
U63682
U63683
U63685
Paulus & Tiedemann 2003
AJ427847
Kim et al 1991;
Wistow et al 1988
M55132
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Buchholz, W. G., Pearce, J. M., Pierson, B. J. & Scribner, K. T. (1998) Dinucleotide repeat polymorphisms in waterfowl (family Anatidae): characterization of a sex-linked
(Z-specific) and 14 autosomal loci. Animal Genetics 29, 323-325
Carew, P. J., Adcock, G. J. & Mulder, R. A. (2003) Microsatellite loci for paternity assessment in the black swan (Cygnus atratus: Aves). Molecular Ecology Notes 3, 1-3
Denk, A. G., Gautschi, B., Carter, K. & Kempenaers, B. (2004) Seven polymorphic microsatellite loci for paternity assessment in the mallard (Anas platyrhynchos).
Molecular Ecology Notes 4, 506-508
Guay, P.-J. & Mulder, R. A. (2005) Isolation and characterization of microsatellite markers in musk duck (Biziura lobata: Aves), and their application to other waterfowl
species. Molecular Ecology Notes 5, 249-252
Fields, R. L. & Scribner, K. T. (1997) Isolation and characterization of novel waterfowl microsatellite loci: cross-species comparisons and research applications. Molecular
Ecology 6, 199-202
Kim, R. Y., Lietman, T., Piatigorsky, J. & Wistow, G. J. (1991) Structure and expression of the duck alpha-enolase/tau-crystallin-encoding gene. Gene 103, 193-200
Maak, S., Neumann, K., von Lengerken, G. & Gattermann, R. (2000) First seven microsatellites developed for the Peking duck (Anas platyrhynchos). Animal Genetics 31,
233
Paulus, K. B. & Tiedemann, R. (2003) Ten polymorphic autosomal microsatellite loci for the Eider duck Somateria mollissima and their cross-species applicability among
waterfowl species (Anatidae). Molecular Ecology Notes 3, 250–252
Stai, S. M. & Hughes, C. R. (2003) Characterization of microsatellite loci in wild and domestic Muscovy ducks (Cairinamoschata). Animal Genetics 34, 387-389
Wistow, G. J., Lietman, T., Williams, L. A., Stapel, S. O., de Jong, W. W., Horwitz, J. and Piatigorsky, J. (1988) Tau-crystallin/alpha-enolase: one gene encodes both an
enzyme and a lens structural protein. Journal of Cell Biology 107, 2729-2736
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