Table S1. Segregation analysis of F2 progeny from plants carrying AtLCB2 mutant alleles.
Progeny Genotype (observed/expected)a
a
Parent Genotype
AABB
AaBB
AABb
AaBb
aaBB
aaBb
AAbb
Aabb
aabb
Total
AaBbb,c
8 /5.9
28 /11.8
17/11.8
21/23.5
6 /5.9
5 /11.8
7 /5.9
2 /11.8
0 /5.9
94
PCR genotyping of 94 individual F2 progeny from a double heterozygous parent plant.
b
Uppercase letter indicates the wild-type allele of AtLCB2a (A) and AtLCB2b (B). Lowercase letter indicates the mutant alleles for AtLCB2a
SAIL_706-A05 (a) and AtLCB2b SALK_110242 (b).
c
Chi-square analysis indicates that the observed genotypic ratios are statistically different than the expected genotypic ratio for this population.
Table S2. Segregation analysis of progeny from reciprocal crosses of plants carrying
mutant AtLCB2 alleles.
a
AaBb
AaBB
AABb
Total
aaBb - Femalec,d
102/118
133/118
N/Ae
235
aaBb – Maled,f
0/259
518/259
N/A
518
Aabb - Femalec,d
106/120
N/A
134/120
240
Aabb – Maled,f
0 /263
N/A
525/263
525
Uppercase letter indicates the wild-type allele of AtLCB2a (A) and AtLCB2b (B). Lowercase
letter indicates the mutant alleles for AtLCB2a SAIL_706-A05 (a) and AtLCB2b SALK_110242
(b).
b
Genotypes were determined by the presence of selectable markers for the mutant alleles
c
Flowers from plants with the indicated genotypes were emasculated and pollinated with pollen
from a wild-type plant.
d
Chi-square analysis indicates that the observed genotypic ratios are statistically different than the
expected genotypic ratio for this population.
e
N/A, the designated genotype is not obtainable from the given parent plant.
f
Plants with indicated genotypes were used to pollinate emasculated flowers from wild-type
plants.