CURRICULUM VITAE
Personal Data
Name:
Emilio M. Casanova Hevia
Date of Birth: 25-08-1969
Nationality:
Spain
Address:
Ludwig Boltzmann Institute for Cancer Research (LBI-CR)
Währinger Str. 13a, A-1090, Vienna, Austria
Phone
+43 1 427764111 / 43 1 427764160
Fax
+43 1 4277 9641
Email:
emilio.casanova@lbicr.lbg.ac.at
Homepage
http://lbicr.lbg.ac.at/
Education
Ph.D. Thesis. -1992-1997: “Molecular analysis of the protein kinases JNK/SAPK in mouse”.
Supervisors: Dr. Pedro Calvo Ferandez and Dr. Miguel A. Chinchetru Manero.
Extraordinary award on PhD thesis from University of Leon, Spain (1997).
Diploma Thesis (Tesina) 1992. “Effects of the chronic ethanol treatment on barbiturate
modulation of muscimol binding to GABAA receptors in rat brain cortex”. Supervisors: Dr.
Pedro Calvo Ferandez and Dr. Miguel A. Chinchetru Manero.
Extraordinary award on Diploma thesis from University of Leon, Spain (1992)
Degree in Biology 1987-1992. Faculty of Biology. University of Leon. Spain.
Training work as a student in the Department of Biochemistry and Molecular Biology (1990 –
1992).
Research Experience
2006-present. Group leader position in the Ludwig Boltzmann Institute for Cancer Research,
Vienna, Austria. Our group is interested in modelling human diseases in mice. We make use
of mice genetically modified to study different pathological process such as liver fibrosis and
cancer. Recently we have developed several mouse models for liver fibrosis and we are
currently analyzing them. Also, by making use of mouse genetic technology, we have
generated a mouse model for skin and brain hemangiomas.
2002-2006. Working as a postdoc in the Group of Dr. Bernhard Bettler, Biozentrum, Basel,
Switzerland. My work focused on studying the trafficking of GABA(B) receptors using life
imaging of primary neuronal cultures of genetically engineered mice expressing GABA(B)GFP fusion proteins. Simultaneously, we generated the knock-outs mice of two new
discovered G protein-coupled orphan receptors to study their function on brain development
and synaptic transmission (in collaboration with Novartis).
1997 – 2002. Working as a postdoc in the group of Dr. Günther Schütz, DKFZ, Heidelberg,
Germany. During this period I studied the different signalling pathways in brain that activate
the transcription factor CREB via phosphorylation. For this purpose, I generated transgenic
animals using BAC technology to express constitutive and tamoxifen-inducible Cre
recombinases in brain. I also generated conditional targeted mice of several putative CREB
kinases. During this time I learned several techniques related to mouse genetics, such as
mouse stem cell culture and manipulation, generation of transgenic mice via modified BACs
or PACs, knock-out/knock-ins and conditional knock-out mice models.
1992 – 1997. During my PhD I learnt several techniques in Biochemistry and Molecular
Biology including: Receptors binding assays, autoradiography, DNA cloning, RT-PCR,
cDNA library screening, Southern and Northern blot, in situ hybridisation, preparation and
purification of recombinant proteins, antibody generation, Western blot, ELISA and
Immunohistochemistry.
Publications
42. Mueller KM, Kornfeld JW, Friedbichler K, Blaas L, Egger G, Esterbauer H, Hasselblatt P,
Schlederer M, Haindl S, Wagner KU, Engblom D, Haemmerle G, Kratky D, Sexl V, Kenner
L, Kozlov AV, Terracciano L, Zechner R, Schuetz G, Casanova E, Pospisilik JA, Heim MH,
Moriggl R.
Impairment of hepatic growth hormone and glucocorticoid receptor signaling causes steatosis
and hepatocellular carcinoma in mice.
Hepatology. (in press)
41. Blaas L, Musteanu M, Grabner B, Eferl R, Bauer A and Casanova E.
The Use of Bacterial Artificial Chromosomes for Recombinant Protein Production in
Mammalian Cell Lines
Methods in Molecular Biology (in press)
40. Mair M, Blaas L, Osterreicher CH, Casanova E, and Eferl R.
JAK-STAT signaling in hepatic fibrosis
Front Biosci. 2011 Jun 1;17:2794-811
39 Eckelhart E, Warsch W, Zebedin E, Simma O, Stoiber D, Kolbe T, Rülicke T, Mueller M,
Casanova E*, Sexl V*.
A novel Ncr1-Cre mouse reveals the essential role of STAT5 for NK cell survival and
development.
Blood. 2011 Feb 3;117(5):1565-73.
*Equally contributed
38 Grabner B, Blaas L, Musteanu M, Hoffmann T, Birbach A, Eferl R and Casanova E.
A mouse tool for conditional mutagenesis in ovarian granulosa cells
Genesis 2010 Oct 1;48(10):612-7.
37. Guetg N, Aziz S, Holbro N, Turecek R, Riad S, Gassmann M, Moes S, Jenoe P, Oertner
T, Casanova E and Bettler B
NMDA Receptor-Dependent GABAB Receptor Internalization via CaMKII Phosphorylation
of Serine 867 in GABAB1
PNAS, 2010 Aug 3;107(31)
36 Mair M; Zollner G; Schneller D; Musteanu M; Fickert P; Gumhold J; Schuster C;
Fuchsbichler A; Bilban M; Tauber S; Esterbauer H; Kenner L; Poli V; Blaas L; Kornfeld JW;
Casanova E; Mikulits W; Trauner M; Eferl R.
Stat3 protects from liver injury and fibrosis in a mouse model of sclerosing cholangitis
Gastroenterology. 2010 Feb 26
35 Musteanu M, Blaas L, Mair M, Schlederer M, Bilban M, Tauber S, Esterbauer H, Mueller
M, Casanova E, Kenner L, Poli V and Eferl R1
Stat3 is a negative regulator of intestinal tumor progression in ApcMin mice
Gastroenterology. 2010 Mar;138(3):1003-11
34. Blaas L, Kornfeld JW, Schramek D, Musteanu M, Zollner G, Gumhold J, Schneller D,
Esterbauer H, Mair M, Kenner L, Mikulits W, Eferl R, Moriggl R, Penninger J, Trauner M
and Casanova E.
Disruption of the GH-STAT5-IGF-1 axis severely aggravates liver fibrosis in a mouse model
of cholestasis
Hepatology. 2010 Apr;51(4):1319-260
33. Birbach A, Casanova E and. Schmid JA.
A Probasin-MerCreMer BAC allows inducible recombination in the mouse prostate
Genesis 2009 Nov 47
32. Casanova E*, Guetg N*, Vigot R, Seddik R, Julio-Pieper M, Hyland NP, Cryan JF,
Gassmann M and Bettler B.
A Mouse Model for Visualization of GABA(B) Receptors.
Genesis (2009) Jul 14
*Equal contribution
31. Blaas L, Musteanu M, Eferl R, Bauer A and Casanova E.
Bacterial artificial chromosomes improve recombinant protein production in mammalian
cells.
BMC Biotechnol. (2009) Jan 14;9(1):3.
30. Wellendorph P, Johansen L, Jensen A, Casanova E, Gassmann M, Deprez P, ClementLacroix P, Bettler B and Bräuner-Osborne H.
No evidence for a bone phenotype in GPRC6A knockout mice under normal physiological
conditions.
J Mol Endocrinol. (2008) Dec 22.
29. Bentzinger CF, Romanino K, Cloëtta D, Lin S, Mascarenhas JB, Oliveri F, Xia J,
Casanova E, Costa CF, Brink M, Zorzato F, Hall MN and Rüegg MA
Skeletal Muscle-Specific Ablation of raptor, but Not of rictor, Causes Metabolic Changes and
Results in Muscle Dystrophy.
Cell Metab. (2008) Nov;8(5):411-24.
28. Maison SF, Casanova E, Holstein GR, Bettler B and Liberman MC
Lack of GABAB receptors in cochlear neurons suggests modulation of outer hair cell function
by type-II afferent fibers
J Assoc Res Otolaryngol (2008) Oct 17
27. Blaas L., Musteanu M., Zenz R., Eferl R. and Casanova E.
PhiC31 Mediated Cassette Exchange into a BAC
Biotechniques. (2007) Nov;43(5):659-60, 662, 664.
26. Lemberger T, Parlato R, Dassesse D, Westphal M, Casanova E, Turiault M, Tronche F,
Schiffmann SN, Schutz G.
Expression of Cre recombinase in dopaminoceptive neurons.
BMC Neurosci. (2007) Jan 3;8(1):4
25. Yuan X, Zhou Y, Casanova E, Chai M, Kiss E, Grone HJ, Schutz G, Grummt I.
Genetic Inactivation of the Transcription Factor TIF-IA Leads to Nucleolar Disruption, Cell
Cycle Arrest, and p53-Mediated Apoptosis.
Mol Cell. 19 (2005) 77-87.
24. Alberti, S., Krause, SM., Kretz,O., Philippar, U., Lemberger,T., Casanova, E., Wiebel,
FF., Schwarz, H., Frotscher, M., Schütz,G., and Nordheim, A.
Neuronal migration in the murine rostral-migratory stream requires serum response factor
Proc Natl Acad Sci U S A. 102 (2005) 6148-53
23. Callejo AI, Casanova E, Calvo P, Galetto R, Rodriguez-Rey JC, Chinchetru MA.
Characterization of the promoter of the mouse c-Jun NH(2)-terminal/stress-activated protein
kinase alpha gene.
Biochim Biophys Acta. 24 (2004) 47-52
22. Z. Zhang, C. Hofmann, E. Casanova, G. Schütz and B. Lutz
Generation of a conditional allele of the CBP gene in mouse
Genesis 40 (2004) 82-89
21. C. Haller*, E. Casanova*, M. Müller*, C. Vacher, R. Vigot, S. Barbieri, M. Gassmann,
and B. Bettler
A Floxed Allele for Conditional Inactivation of the GABAB(1) Gene
Genesis 18 (2004) 125-130
*Equal contribution
20. Marsicano G, Goodenough S, Monory K, Hermann H, Eder M, Cannich A, Azad SC,
Cascio MG, Gutierrez SO, van der Stelt M, Lopez-Rodriguez ML, Casanova E, Schutz G,
Zieglgansberger W, Di Marzo V, Behl C, Lutz B.
CB1 cannabinoid receptors and on-demand defense against excitotoxicity.
Science 302 (2003) 84-88
19. E. Casanova, S. Fehsenfeld, T. Lemberger and G. Schütz
Alpha complementation in the Cre recombinase enzyme
Genesis 37 (2003) 25-29
18. F. Tronche, E. Casanova, M. Turiault, I. Sahly, C. Kellendonk
When reverse genetics meets physiology: the use of site-specific recombinases in mice
FEBS Lett. 529 (2002)116-121
17. E. Casanova*, S. Fehsenfeld, T. Lemberger, D. R. Shimshek, R. Sprengel and T.
Mantamadiotis
A Er-based Double iCre Fusion Protein Allows Partial Recombination in Forebrain
Genesis 34 (2002) 208-214
*Corresponding author
16. E. Casanova, S. Fehsenfeld, E. Greiner, A. F. Stewart and G. Schütz
Conditional Mutagenesis of CamKIV
Genesis 32 (2002) 161-164
15. E. Casanova, S. Fehsenfeld, E. Greiner, A. F. Stewart and G. Schütz
Construction of a Conditional Allele of RSK-B/MSK2 in the mouse
Genesis 32 (2002) 158-160
14. D.R. Shimshek, J. Kim, M.R. Hübner, D.J. Spergel, F. Buchholz, E. Casanova, A.F.
Stewart, P.H. Seeburg and R. Sprengel
Codon-improved Cre recombinase (iCre) expression in the mouse
Genesis 32 (2002) 19-26
13. E. Casanova, T. Lemberger, S. Fehsenfeld, E. Greiner and G. Schütz
Rapid localization of a gene within BACs and PACs
Biotechniques 32 (2002) 240-242
12. E. Casanova, S. Fehsenfeld, T. Mantamadiotis, T. Lemberger, E. Greiner, A. F. Stewart,
G. Schütz
allows brain-specific gene inactivation
Genesis 31 (2001) 37-42
11. E. Casanova, A.I. Callejo, P. Calvo P, M.A. Chinchetru
Analysis of splicing of four mouse JNK/SAPKalpha variants.
Neuroreport 11 (2000) 305-309
10. M.A. Chinchetru, E. Casanova and P.Calvo
Stress-activated protein kinases ( JNK/SAPKs) of mammalian brain
Trends in Comparative Biochem.and Physiol. 4 (1998) 255-259
9. C. Kellendonk, F. Tronche, E. Casanova, K. Anlag, C. Opherk and G. Schütz
Inducible Site-specific Recombination in the Brain
J. Mol. Biol. 285 (1999)175-182
8. A. Alonso-Llamazares, J. Lopez-Alonso, M. del Barrio, E. Casanova, P. Calvo, MA.
Chinchetru.
Cloning of chicken and mouse a1b adrenergic receptor
Biochim. Biophys. Acta 1396 (1998) 263-266
7. A. Alonso-Llamazares, E. Casanova , D. Zamanillo, S. Ovalle, P.Calvo and MA:
Chinchetru.
Phosphorylation of the third intracellular loop of the mouse a1b- Adrenergic receptor by
cAMP-dependent protein kinase.
Brain Res. Bull. 42 (1997) 427-430
6. E. Casanova, C. Garate, S. Ovalle, P.Calvo and MA. Chinchetru.
Identification of four splice variants of the mouse stress activated protein kinase JNK/SAP a
isoform.
NeuroReport 7 (1996) 1320-1324
5. E. Casanova, A. Alonso-Llamazares, D. Zamanillo, C. Garate, P. Calvo and MA.
Chinchetru.
Identification of a long huntingtin mRNA transcript in mouse brain.
Brain Res. 743 (1996) 320-323
4. S. Ovalle, E. Casanova, C. Garate, A. Alonso-Llamazares, MA. Chinchetru and P. Calvo
Immunodetection of serotonin transporter from mouse brain.
NeuroReport, 6 (1995) 2353-2356
3. A. Alonso-Llamazares, D. Zamanillo, E. Casanova, S. Ovalle, P. Calvo, M.A, Chinchetru.
Molecular cloning of a1d-adrenergic receptor and tissue distribution of tree a1-adrenergic
receptors subtypes in mouse.
J. Neurochem. 65 (1995) 2387-2392
2. D. Zamanillo, E. Casanova, A. Alonso-Llamazares, S. Ovalle, MA. Chinchetru and P.
Calvo.
Identification of a cyclic adenosine 3´, 5´-monophosphate-dependent protein kinase
phosphorylation site in the carboxy terminal tail of D1 dopamine receptor.
Neurosci. Lett. 188 (1995) 183-186
1. M. Negro, E. Casanova. M.A. Chinchetru, A. Fernadez-Lopez and P Calvo.
Differential effect of chronic ethanol treatment on barbiturate and steroid modulation of
muscimol binding to rat brain cortex.
Neurosci. Lett. 158 (1993) 83-86
Grants:
Period
Organization
Short title
€/year
20092012
Austromouse
BAC recombineering to generate compound
Stat3/Stat5 double knock-out mice and
functions of Stat3 in colorectal cancer
61.333
20112014
FFG-Bridge
BAC-based Expression System Technology
177.000
Patents:
Title: “Method fort he generation of a non-human animal with an oncogene”
Application number: 07450118.0-2405
Date: 2.07.07
Applicant/Proprietor: Ludwig Boltzmann Gesellschaft GmbH
Inventors : Robert Eferl, Emilio Casanova, Dagmar Stoiber, Richard Moriggl, johannes
Schmid, Lukas Kenner, Rainer Zenz, Monica Musteanu and Leander Blaas
Title: “Artificial Chromosome Vector”
Application number A 1859/2008
Date 28.11.2008
Applicant/Proprietor:Emilio Casanova, Anton Bauer
Inventors: Leander Blass, Anton Bauer, Emilio Casanova
Seminars and conferences
Invited speaker: Controlling gene expression inducible in mice: conditional mutagenesis,
inducible expression and RNAi. October 2004 Marseille, France
EMBL Mouse Molecular Genetics Meeting, September 2005, EMBL, Heidelberg, Germany
Invited speaker: “Conditional Gene Targeting”
March, 2005 Leibniz Institute for Age Research – Fritz-Lipmann-Institute. Jena, Germany
Invited speaker: 11th Scientific Symposium of Austrian Pharmacology Society (APHAR)
Vienna September 2005
Invited speaker: "Life Imaging of GABA(B) Receptors"
February, 2007 Leibniz Institute for Age Research – Fritz-Lipmann-Institute. Jena, Germany
Invited speaker:"Analysis of GABA(B) Receptors"
February, 2007. Leibniz Institute for Age Research – Fritz-Lipmann-Institute. Jena, Germany
Invited speaker: “Life imaging of GABA(B) Receptors in vivo”
October 2007. Instituto de Biología y Genética Molecular, Valladolid, Spain.
Invited speaker: “Life imaging of GABA(B) Receptors in vivo”
May 2008. Instituto de neurociencias, Alicante, Spain .
Invited speaker: “Role of Stat5 in liver fibrosis”
September 2008. “Research Retreat in Sopron”, Hungary.
Invited speaker: “Role of Stat5 in liver fibrosis”
January 2009. Collège de France, Paris, France.
Invited speaker: “Role of Stat5 in liver fibrosis”
April 2009. “Research Retreat Vienna”, Austria.
Invited speaker: “Role of Stat5 in liver fibrosis”
April, 2009. Faculty of Pharmaceutical Sciences, Copenhagen, Denmark.
Invited speaker: “Role of Stat5 in liver fibrosis”
May 2010. Leibniz Institute for Age Research – Fritz-Lipmann-Institute. Jena, Germany.
Invited speaker: “Mouse models for cancer and liver fibrosis”
September 2010. “Research Retreat in Sopron”, Hungary.
Invited speaker; life imaging of GABAB Patras
October 2010
References:
Prof. Miguel Chinchetru
University of Leon
Leon
Spain
Phone: +34 987 29 1223
Email; dbbmcm@unileon.es
Prof. Günther Schütz
German Cancer Research Center
Heidelberg
Germany
Phone: +49 6221 42 3422
Email: g.schuetz@dkfz.de
Prof. Bernhard Bettler
Pharmazentrum/Biozentrum
University of Basel
Basel
Switzerland
Phone +41 61 267 16 32
Email bernhard.bettler@unibas.ch
Prof. Markus A. Rüegg
Neurobiology
Biozentrum, University of Basel
Klingelbergstrasse 50/70
CH-4056 Basel / Switzerland
Phone +41 61 267 22 23
Email markus-a.ruegg@unibas.ch