PATHOLOGY AND LABORATORY MEDICINE
DIVISION OF TRANSFUSION MEDICINE
STANDARD WORK INSTRUCTION MANUAL
Preparation of Chloroquine Diphosphate Reagent
Approved By: Dr. Antonio Giulivi
Date Issued: 2004/04/05
Date Revised: 2009/12/31
1.0
Document No: RP.006
Category: Reagent Preparation
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Principle
Red cells with a positive DAT cannot be tested accurately with blood
typing reagents that require an indirect antiglobulin technique. 9.1
Chloroquine diphosphate dissociates IgG from the red cell membrane with
little or no damage to its integrity if used under controlled conditions.
2.0
Scope and Related Policies
2.1
Treating IgG coated red cells with chloroquine diphosphate permits
complete phenotyping if anti-sera is reactive by indirect antiglobulin
technique.9.1
2.2
Use of chloroquine diphosphate to dissociate autoantibody from red
cells can aid in investigation of autoabsorption techniques.
3.0
Specimen – N/A
4.0
Material
5.0
Equipment:
Weigh scale
Glass beaker or flask
Supplies:
Weigh boats
Glass reagent bottle
Serological pipettes
pH strips
Reagents:
Normal saline
1N NaOH
Chloroquine Disphosphate salt
Quality Control – N/A
Ontario Regional Blood Coordinating Network
Standard Work Instruction Manual
RP.006
Page 1 of 2
Preparation of Chloroquine Diphosphate Reagent
6.0
Procedure
6.1
Dissolve 20 g of chloroquine diphosphate in 100 mL of normal
saline.
6.2
Adjust pH to 5.1 with 1 N NaOH.
6.3
Label and store in glass reagent bottle at 2-6° C for 6 months.
7.0
Reporting – N/A
8.0
Procedural Notes – N/A
9.0
References
9.1
Roback JD, ed. American Association of Blood Banks Technical
Manual, 16th ed. Bethesda, MD: American Association of Blood
Banks, 2008: 894-895.
Ontario Regional Blood Coordinating Network
Standard Work Instruction Manual
RP.006
Page 2 of 2