Identification and expression analysis of ethylene biosynthesis and signaling genes provide
insights on the early and late coffee cultivars ripening pathway
Solange A Ságioa, Horllys G Barretoa, André A Limaa, Rafael O Moreira a, Pamela M Rezende a,
Luciano V Paivab , Chalfun-Júnior Aa*
aPlant
Molecular Physiology Laboratory, Biology Department, Federal University of Lavras
(UFLA), s/n - Cx. P 3037-37200-000 Minas Gerais, Brazil.
bChemistry
Department, Central Laboratory of Molecular Biology (LCBM), UFLA, s/n - Cx. P 303737200-000- Minas Gerais, Brazil.
*Corresponding author at: Plant Molecular Physiology Laboratory, Biology Department, Federal
University of Lavras (UFLA), s/n - Cx. P 3037- Minas Gerais, Brazil. Fax: +55-35-3829-1100 - Email address: chalfunjunior@dbi.ufla.br (A. Chalfun-Jú nior)
Suppl. Table 1 Comparison of the coffee ethylene biosynthesis and signaling genes identified in
this work with the closest members (clustered in the same clade of the phylogenetic tree (Figure
1)) from the Arabidopsis and tomato ethylene biosynthesis and signaling genes. Coffee sequences
marked with an asterisk (*) represent full length cDNAs. The entire sequence from incomplete
sequences was used in the aligments
Gene
Contig/aa
ACS
CaACS1/231
ACO
ETR
E-value
Identity
Similarity
2e-130
167/215(78%)
193/215(89%)
SlACS1B
(AAF97615)
1e-128
163/215(76%)
192/215(89%)
SlACS6
(BAA34923)
1e-127
169/214(79%)
189/214(88%)
SlACO1
(P05116)
0.0
271/319(85%)
299/319(93%)
SlACO2
(P07920)
0.0
261/320(82%)
289/320(90%)
SlACO3
(P24157)
0.0
272/320(85%)
298/320(93%)
CaACO4/318*
SlACO4
(BAA34924)
0.0
262/318(82%)
290/318(91%)
CaACO5/292*
SlACO5
(AJ715790.1)
1e-144
194/289(67%)
222/289(76%)
AtACO1
(NP179549)
2e-138
185/278(67%)
222/278(79%)
AtETR1
(P49333)
0.0
522/617(85%)
569/617(92%)
SlETR1
(Q41342)
0.0
530/617(86%)
567/617(91%)
CaACO1/319*
CaETR1/615
blastp
SlACS1A
(AAF97614)
CaETR3/246
SlETR2
(O49187)
0.0
514/614(84%)
558/614(90%)
SlETR3
(Q41341)
1e-100
162/247(66%)
200/247(80%)
8e-95
160/247(65%)
195/247(78%)
AtERS1
(Q38846)
CTR1
EIN2
EIN3
ERF
CaETR4/262
SlETR4
(Q9XET8)
1e-141
201/259(78%)
229/259(88%)
CaCTR2/423
SlCTR2
(NP_001234768)
3e-35
55/93(59%)
75/93(80%)
SlCTR1
(AAL87456)
5e-25
43/79(54%)
56/79(70%)
SlCTR3
(NP_001234454)
2e-26
46/83(55%)
56/83(67%)
SlCTR4
(NP_001234457)
1e-20
38/83(46%)
49/83(59%)
AtCTR1
(NP_195993)
4e-23
40/69(58%)
50/69(72%)
SlEIN2
(AAZ95507)
0.0
288/474(61%)
361/474(76%)
AtEIN2
(AAD41076)
3e-128
225/494(46%)
316/494(63%)
SlEIL1
(Q94FV4)
0.0
465/634(73%)
531/634(83%)
SlEIL4
(Q76DI3)
0.0
462/635(73%)
527/635(82%)
SlEIL2
(Q94FV3)
0.0
457/634(72%)
530/634(83%)
CaERF1/304*
SLERF78
3e-55
109/223(49%)
131/223(58%)
CaERF2/304
SLERF79
135/299(45%)
169/299(56%)
CaEIN2/483
CaEIN3/644*
7e-65
SLERF80
1e-46
116/263(44%)
142/263(53%)
CaERF3/387
SLERF77
2e-117
198/389(51%)
258/389(66%)
CaERF4/165
SLERF35
7e-28
54/65(83%)
56/65(86%)
CaERF5/404*
SLERF77
6e-129
216/405(53%)
273/405(67%)
CaERF6/220*
SLERF35
5e-59
119/191(62%)
134/191(70%)
CaERF7/226
SLERF35
4e-21
49/73(67%)
56/73(76%)