Tissue Engineers, like gene therapists, are working toward a technological solution to the challenge of gene delivery. A number of strategies have been employed to ferry DNA into host cells, including viral vectors. More recently, scientists have tried to employ artificial vectors, some of which are lipid vesicle vectors known as liposomes. In this investigation, your company is challenged to assess the potential of 5 different liposome formulas. The liposome formula that produces the largest stable “bubble” dome will be judged the most effective formula.
1.
Create liposome solution by mixing 2mL of liposome formula with 100mL of tap water.
2.
Transfer 10mL of solution to the lab table surface and create a large round smear as directed.
3.
Dip a straw into the solution and generate a “bubble dome.” When the dome breaks, measure the diameter. If irregular, average 2-3 diameters.
4.
Repeat at least 4 more times.
5.
Graph your results, and perform appropriate statistical analysis. Interpret.
BONUS: To more clearly reflect the ‘in vivo’ environment of the host, devise and perform a suitable investigation.
Questions:
Do the liposome formulas vary in stability? What is your evidence? Explain.
Which formula(s) varies significantly from the control? Explain.