Bioinformatics and Expression Analysis core

advertisement

Illumina MiSeq Instrument NOW at BEA

BEA is now introducing the Illumina

MiSeq, the next generation DNA sequence reader with a single lane flowcell capable of generating 15 million single end reads (SR) or 30 million paired end reads (PE).

Illumina prepared libraries are loaded directly to the MiSeq instrument where amplified clusters are generated on the flowcell and sequencing by synthesis takes place. Reagent kits for 50, 300 and 500 cycles are available.

The goal of BEA is to provide complete sequence analysis including preparation of sequencing libraries, sequencing and support for data analysis and bioinformatics for smaller sequencing projects. It is important to emphasize that the sequencing activities at BEA should be seen as complementary to the well-established high throughput sequencing activities provided by SciLife Lab in Solna and Uppsala where the activities in Huddinge are characterized by our ambition to provide a broad repertoire of genomic platforms and applications.

Good Results using Agilent Microarrays

BEA has recently run some Agilent microarrays for the first time with very good results. If you’re interested in running Agilent microarrays at BEA, please contact Susann Fält .

Rhesus Macaque v2 Gene Expression

The Agilent Rhesus Macaque v2 array includes more than 17,000 distinct rhesus macaque transcripts, approximately 1,000 human genes, and 96 viral genes (representing 27 different viruses).

Rhesus Macaque and other Agilent model organism microarrays

Medicago Gene Expression

BEA has run the first plant microarray from

Agilent. The array uses the 4*44K high-definition format. Other plant arrays from Agilent include

Arabidopsis, Barley, Brassica and Cotton.

Medicago and other Agilent model organism microarrays

Human miRNA Expression

SurePrint Human miRNA Microarrays provide a comprehensive coverage of miRNAs with the most up-to-date content. A proprietary probe design and unique labeling method provide optimal sensitivity and specificity.

Agilent Human miRNA Microarrays

Methylation Seminar August

If you are interested in DNA Methylation analysis don’t forget to mark your calendars for the upcoming event: “Illumina 450K - DNA Methylation analysis in humans”.

BEA is co-organizing a half day seminar the 27th August together with the

Unit for Computational Medicine and Illumina.

Attendance at the seminar is free.

Seminar Invitation

Registration page

Summer vacation

BEA will be partly closed week 30-31 (July 22 - August 2). The core facility staff will be reduced during the rest of the summer, and we strongly recommend visitors to contact the core facility before visiting.

Make sure that we are here before visiting.

We wish you all a very nice summer!

Bioinformatics and Expression Analysis core facility (BEA)

Fredrik Fagerström-Billai Group Leader

Marika Rönnholm Research Engineer fredrik.fagerstrom-billai@ki.se

marika.ronnholm@ki.se

Malin Nilsson

Susann Fält

Jessica Lindvall

David Brodin

Research Engineer

Bioinformatician

Bioinformatician malin.nilsson@ki.se

Research Engineer susann.falt@ki.se

david.brodin@ki.se

Jessica.lindvall@ki.se

Visiting Address

Department of Biosciences and Nutrition at NOVUM,

Karolinska Institutet,

SE-141 57 Huddinge, SWEDEN

Homepage: www.bea.ki.se

Download