朱健康
中科院上海植物逆境生物学研究中心 主任
首席科学家、资深研究员，博士生导师 ，美国国家科学院院士
中组部“千人计划”顶尖人才入选者
电话：(86) 21-57078201
Email: jkzhu@sibs.ac.cn
研究方向：
植物逆境生物学
研究工作：
Detecting and responding to environmental perturbations are important for all living organisms. One of
the most important distinguishing features of plants is that they are sessile and thus have to endure
environmental challenges. Our lab is interested in the molecular mechanisms underlying plant responses
to harsh environments such as soil salinity, drought and cold temperatures. In addition, we are interested
in the mechanisms of transcriptional gene silencing and in the role of epigenetic gene regulation in stress
adaptation. We use a combination of genetic, biochemical, genomic and proteomic approaches to
analyze various levels of gene regulation (chromatin level/epigenetic, transcriptional, posttranscriptional,
and protein activity) and to understand stress signaling and stress tolerance. Our long-term goals are to
elucidate the signaling pathways used by plants in responding to environmental stresses and to identify
key genes for modifying the responses of crops to environmental stresses which ultimately will lead to
major contributions to agriculture and the environment.
个人简介：
1983-1987 年，北京农业大学，获土壤农业化学学士学位；
1987-1990 年，美国加州大学河滨分校，获植物学硕士学位；
1990-1993 年，美国普度大学，获植物生理学博士学位；
1994 年，美国洛克菲勒大学，获分子生物学博士后；
1995-1996 年，美国奥本大学植物与微生物学系担任助理教授；
1996-1998 年，美国亚利桑那大学植物科学系担任助理教授；
1999-2000 年，美国亚利桑那大学植物科学系担任副教授、2000 年提升为教授；
2002 年， 获得亚利桑那大学生命科学与农学院年度最佳研究员奖；
2003 年，荣获美国植物生物学会颁发的 Charles Albert Shull 大奖；
2004-2007 年，美国加州大学河滨分校植物学与植物科学系担任特聘教授、研究所所长；
2007-2010 年，美国加州大学河滨分校植物学与植物科学系担任 Jane Johnson 讲座教授；
2010-至今，美国普度大学生物化学与园艺及园林系杰出教授；
2010 年，当选美国科学院院士；
2011 年，入选首批中央“千人计划”顶尖人才团队，中国科学院上海植物逆境生物学研究中心首席科学家。
代表性论文：
Zhaobo Lang, Mingguang Lei, Xingang Wang, Kai Tang, Daisuke Miki, Huiming Zhang, Satendra K.
Mangrauthia, Wenshan Liu, Wenfeng Nie, Guojie Ma, Jun Yan, Cheng-Guo Duan, Chuan-Chih Hsu,
Chunlei Wang, W. Andy Tao, Zhizhong Gong, Jian-Kang Zhu. The Methyl-CpG-Binding Protein MBD7
Facilitates Active DNA Demethylation to Limit DNA Hyper-Methylation and Transcriptional Gene
Silencing. Molecular Cell. doi:10.1016/j.molcel.2015.01.009.
Zhaobo Lang, Mingguang Lei, Xingang Wang, Kai Tang, Daisuke Miki, Huiming Zhang, Satendra K.
Mangrauthia, Wenshan Liu, Wenfeng Nie, Guojie Ma, Jun Yan, Cheng-Guo Duan, Chuan-Chih Hsu,
Chunlei Wang, W. Andy Tao, Zhizhong Gong, Jian-Kang Zhu. . The Methyl-CpG-Binding Protein MBD7
Facilitates Active DNA Demethylation to Limit DNA Hyper-Methylation and Transcriptional Gene
Silencing. Molecular Cell. doi:10.1016/j.molcel.2015.01.009.
Zhaobo Lang, Mingguang Lei, Xingang Wang, Kai Tang, Daisuke Miki, Huiming Zhang, Satendra K.
Mangrauthia, Wenshan Liu, Wenfeng Nie, Guojie Ma, Jun Yan, Cheng-Guo Duan, Chuan-Chih Hsu,
Chunlei Wang, W. Andy Tao, Zhizhong Gong, Jian-Kang Zhu. . The Methyl-CpG-Binding Protein MBD7
Facilitates Active DNA Demethylation to Limit DNA Hyper-Methylation and Transcriptional Gene
Silencing. Molecular Cell. doi:10.1016/j.molcel.2015.01.009.
Yan Li, Dolores Córdoba-Cañero, Weiqiang Qian, Xiaohong Zhu, Kai Tang, Huiming Zhang, Rafael R.
Ariza, Teresa Roldan-Arjona，Jian-Kang Zhu. An AP endonuclease functions in active DNA
dimethylation and gene imprinting in Arabidopsis. Plos Genetics 11(1):e1004905.
Hui Zhang1, Jinshan Zhang1,2, Pengliang Wei1,2, Botao Zhang1, Feng Gou1,2, Zhengyan Feng1,2,3, Yanfei
Mao1, Lan Yang1, Heng Zhang1, Nanfei Xu1,* andJian-Kang Zhu1,4,*. The CRISPR/Cas9 system
produces specific and homozygous targeted gene editing in rice in one generation Plant Biotechnology
Journal Article first published online: 23 MAY 2014 DOI: 10.1111/pbi.12200.
Weiqiang Qian*, Daisuke Miki*, Mingguang Lei*, Xiaohong Zhu, Huiming Zhang, Yunhua Liu, Yan Li,
Zhaobo Lang, Jing Wang, Kai Tang, Renyi Liu, Jian-Kang Zhu. Regulation of Active DNA Demethylation
by an α-Crystallin Domain Protein in ArabidopsisMolecular Cell DOI:
http://dx.doi.org/10.1016/j.molcel.2014.06.008.
Chizuko Yamamuro，Daisuke Miki,Zhimin Zheng,Jun Ma,Jing Wang,Zhenbiao Yang,Juan Dong&
Jian-Kang Zhu. Overproduction of stomatal lineage cells in Arabidopsis mutants defective in active DNA
demethylation Nature Communications. 5， Article number: 4062 doi:10.1038/ncomms5062 Received
03 March 2014 Accepted 07 May 2014 Published 05 June 2014.
Yang Zhao1,2*, Lu Xing2,3*, Xingang Wang2, Yueh-Ju Hou2, Jinghui Gao2,4, Pengcheng Wang2,
Cheng-Guo Duan2, Xiaohong Zhu2, and Jian-Kang Zhu1,2. The ABA Receptor PYL8 Promotes Lateral
Root Growth by Enhancing MYB77-Dependent Transcription of Auxin-Responsive Genes Sci. Signal.，3
June 2014 Vol. 7， Issue 328， p. ra53 DOI: 10.1126/scisignal.2005051.
Zhengyan Fenga,b,c,1, Yanfei Maoa,1, Nanfei Xua, Botao Zhanga, Pengliang Weia,c, Dong-Lei Yanga, Zhen
Wanga,c, Zhengjing Zhanga,c, Rui Zhenga,c, Lan Yanga, Liang Zenga, Xiaodong Liua, and Jian-Kang
Zhua,d,2. Multigeneration analysis reveals the inheritance， specificity， and patterns of
CRISPR/Cas-induced gene modifications in Arabidopsis. PNAS. doi: 10.1073/pnas.1400822111.
Mingguang Lei*, Honggui La*, Kun Lu, Pengcheng Wang, Daisuke Miki, Zhizhong Ren, Cheng-Guo
Duan, Xingang Wang, Kai Tang, Liang Zeng, Lan Yang, Heng Zhang, Wenfeng Nie, Pan Liu, Jianping
Zhou, Renyi Liu, Yingli Zhong, Dong Liu, and Jian-Kang Zhu. Arabidopsis EDM2 promotes IBM1 distal
polyadenylation and regulates genome DNA methylation patterns. Proc Natl Acad Sci U S A. 2014 Jan
7;111(1):527-32.
Yang Zhao1,2,*, Zhulong Chan1,3,*, Lu Xing2,4, Xiaodong Liu1, Yueh-Ju Hou2, Viswanathan Chinnusamy2,5,
Pengcheng Wang2, Chengguo Duan2 and Jian-Kang Zhu1,2. . The unique mode of action of a divergent
member of the ABA-receptor protein family in ABA and stress signaling. Cell Res. 2013
Dec;23(12):1380-95.
Ting Ban1, Jiyuan Ke2, Runze Chen3, Xin Gu2, M. H. Eileen Tan2, X. Edward Zhou2, Yanyong Kang2,
Karsten Melcher2, Jian-Kang Zhu4 and H. Eric Xu5*. . Structure of a PLS-Class Pentatricopeptide
Repeat Protein Provides Insights into Mechanism of RNA Recognition. J Biol Chem. 2013 Nov
1;288(44):31540-8.
Chao-Feng Huang1,2, Daisuke Miki1, Kai Tang3, Hao-Ran Zhou4, Zhimin Zheng1, Wei Chen3, Ze-Yang
Ma4, Lan Yang1, Heng Zhang1, Renyi Liu5, Xin-Jian He4, Jian-Kang Zhu1,3,*. . A Pre-mRNA-Splicing
Factor Is Required for RNA-Directed DNA Methylation in Arabidopsis PLoS Genet. 2013;9(9):e1003779.
Yanfei Mao, Hui Zhang, Nanfei Xu, Botao Zhang, Feng Gou and Jian-Kang Zhu.. Application of the
CRISPR-Cas system for efficient genome engineering in plants. Mol Plant. 2013 Nov;6(6):2008-11.
Zhengyan Feng1,2,3,*, Botao Zhang1,*, Wona Ding4, Xiaodong Liu1, Dong-Lei Yang1, Pengliang Wei1,
Fengqiu Cao1, Shihua Zhu4, Feng Zhang5, Yanfei Mao1, Jian-Kang Zhu1,6. . Efficient genome editing in
plants using a CRISPR/Cas system. Cell Res. 2013 Oct;23(10):1229-32.
Kun Dou1,2,#, Chao-Feng Huang3,#, Ze-Yang Ma1, Cui-Jun Zhang1, Jin-Xing Zhou1, Huan-Wei Huang1,
Tao Cai1, Kai Tang3,Jian-Kang Zhu3,4 and Xin-Jian He1,*. . The PRP6-like splicing factor STA1 is
involved in RNA-directed DNA methylation by facilitating the production of Pol V-dependent scaffold
RNAs. Nucleic Acids Res. 2013 Oct;41(18):8489-502.
Hao-Ran Zhoua,b,1, Fang-Fang Zhanga,1, Ze-Yang Maa, Huan-Wei Huanga, Ling Jiangc, Tao
Caia, Jian-Kang Zhud,e, Chuyi Zhangc and Xin-Jian Hea,b,2. . Folate Polyglutamylation Is Involved in
Chromatin Silencing by Maintaining Global DNA Methylation and Histone H3K9 Dimethylation in
Arabidopsis. Plant Cell. 2013 Jul;25(7):2545-59.
Minjie Cao1,*, Xue Liu1,2,*, Yan Zhang3, Xiaoqian Xue3,4, X Edward Zhou5, Karsten Melcher5, Pan Gao1,
Fuxing Wang1, Liang Zeng1, Yang Zhao6, Yang Zhao7, Pan Deng8, Dafang Zhong8, Jian-Kang Zhu1,6, H
Eric Xu2,5 and Yong Xu3. . An ABA-mimicking ligand that reduces water loss and promotes drought
resistance in plants. Cell Res. 2013 Aug;23(8):1043-54.
Heng Zhanga,1, Ze-Yang Mab,1, Liang Zenga, Kaori Tanakac, Cui-Jun Zhangb, Jun Maa, Ge Baia,
Pengcheng Wangd, Su-Wei Zhangb, Zhang-Wei Liub, Tao Caib, Kai Tangd, Renyi Liue, Xiaobing Shic,
Xin-Jian Heb,2, and Jian-Kang Zhua,d,2. . DTF1 is a core component of RNA-directed DNA methylation
and may assist in the recruitment of Pol IV. Proc Natl Acad Sci U S A. 2013 May 14;110(20):8290-5.
Xiaohong Zhu1,*, Ying Feng1, Gaimei Liang1, Na Liu1, Jian-Kang Zhu1,2*. . Aequorin-based
luminescence imaging reveals stimulus- and tissue-specific Ca2+ dynamics in Arabidopsis plants. Mol
Plant. 2013 Mar;6(2):444-55.
Xiangqiang Zhana,1,Bangshing Wangb,1, Hongjiang Lic, Renyi Liuc, Rajwant K. Kaliad, Jian-Kang Zhua,b,2,
and Viswanathan Chinnusamye,2. Arabidopsis proline-rich protein important for development and abiotic
stress tolerance is involved in microRNA biogenesis.Proc. Natl. Acad. Sci. USA 2012;
109(44):18198-203.
Weiqiang Qian1,2,*, Daisuke Miki1,*, Heng Zhang1,2, Yunhua Liu2,3, Xi Zhang4, Kai Tang2, Yunchao Kan5,
Honggui La1,2, Xiaojie Li6, Shaofang Li7, Xiaohong Zhu2, Xiaobing Shi4, Kangling Zhang8, Olga Pontes9,
Xuemei Chen7, Renyi Liu7, Zhizhong Gong6,Jian-Kang Zhu1,2,† . A histone acetyltransferase regulates
active DNA demethylation in Arabidopsis. Science 2012; 336(6087):1445-8.
Xiaojie Lia, Weiqiang Qianb, Yusheng Zhaoa, Chunlei Wanga, Jie Shena, Jian-Kang Zhub,c,1, and
Zhizhong Gonga,d,1.Antisilencing role of the RNA-directed DNA methylation pathway and a histone
acetyltransferase in Arabidopsis. Proc. Natl. Acad. Sci. USA 2012; 109(28):11425-30.
Qian W, Miki D, Zhang H, Liu Y, Zhang X, Tang K, Kan Y, La H, Li X, Li S, Zhu X, Shi X, Zhang K, Pontes
O, Chen X, Liu R, Gong Z, Zhu JK. 2012. A histone acetyltransferase regulates active DNA
demethylation in Arabidopsis. Science 336:1445-8.
Mahfouz MM, Li L, Shamimuzzaman M, Wibowo A, Fang X, Zhu JK. 2011. De novo-engineered
transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates
double-strand breaks. Proc. Natl. Acad. Sci. USA 108:2623-2628.
Quintero FJ, Martinez-Atienza J, Villalta I, Jiang X, Kim WY, Ali Z, Fujii H, Mendoza I, Yun DJ, Zhu JK,
Pardo JM. 2011. Activation of the plasma membrane Na/H antiporter Salt-Overly-Sensitive 1 (SOS1) by
phosphorylation of an auto-inhibitory C-terminal domain. Proc. Natl. Acad. Sci. USA 108:2611-2616.
Fujii H, Verslues PE, Zhu JK. 2011. Arabidopsis decuple mutant reveals the importance of SnRK2
kinases in osmotic stress responses in vivo. Proc. Natl. Acad. Sci. USA 108:1717-1722.
Gao Z, Liu HL, Daxinger L, Pontes O, He X, Qian W, Lin H, Xie M, Lorkovic ZJ, Zhang S, Miki D, Zhan X,
Pontier D, Lagrange T, Jin H, Matzke AJ, Matzke M, Pikaard CS, Zhu JK. 2010. An RNA polymerase IIand AGO4-associated protein acts in RNA-directed DNA methylation. Nature 465:106-109.
Ren Z, Zheng Z, Chinnusamy V, Zhu J, Cui X, Iida K, Zhu JK. 2010. RAS1, a quantitative trait locus for
salt tolerance and ABA sensitivity in Arabidopsis. Proc. Natl. Acad. Sci. USA 107:5669-5674.
Melcher K, Ng LM, Zhou XE, Soon FF, Xu Y, Chinnusamy V, Suino-Powell KM, Kovach A, Li J, Yong EL,
Zhu JK, Xu HE. 2010. Identification and Mechanism of ABA Receptor Antagonism. Nat. Struct. Mol. Biol.
17:1102-1108.
Liu Q, Wang J, Miki D, Xia R, Yu W, He J, Zheng Z, Zhu JK, Gong Z. 2010. DNA Replication Factor C1
Mediates Genomic Stability and Transcriptional Gene Silencing in Arabidopsis. Plant Cell 22:2336-2352.
Jin H, Zhu JK. How many ways are there to generate small RNAs. 2010. Mol. Cell 38:775-777.
Klingler JP, Batelli G, Zhu JK. 2010. ABA receptors: the START of a new paradigm in phytohormone
signalling. J. Exp. Bot. 61:3199-3210.
Jin H, Zhu JK. 2010. A viral supprossor protein inhibits host RNA silencing by hooking up with Argonautes.
Genes Dev. 24:853-856.
Law JA, Ausin I, Johnson LM, Vashisht AA, Zhu JK, Wohlschlegel JA, Jacobsen SE. 2010. A Protein
Complex Required for Polymerase V Transcripts and RNA- Directed DNA Methylation in Arabidopsis.
Curr. Biol. 20:951-956.
Zhu J, Ha Lee B, Dellinger M, Cui X, Zhang C, Wu S, Nothnagel EA, Zhu JK. 2010. A cellulose
synthase-like protein is required for osmotic stress tolerance in Arabidopsis. Plant J. 63:128-140.
Yao Y, Ni Z, Peng H, Sun F, Xin M, Sunkar R, Zhu JK, Sun Q. 2010. Non-coding small RNAs responsive
to abiotic stress in wheat (Triticum aestivum L.). Funct. Integr. Genomics. 10:187-190.
Fedoroff NV, Battisti DS, Beachy RN, Cooper PJ, Fischhoff DA, Hodges CN, Knauf VC, Lobell D, Mazur
BJ, Molden D, Reynolds MP, Ronald PC, Rosegrant MW, Sanchez PA, Vonshak A, Zhu JK. 2010.
Radically rethinking agriculture for the 21st century. Science 327:833-834.
Zheng Z, Xing Y, He XJ, Li W, Hu Y, Yadav SK, Oh J, Zhu JK. 2010. An SGS3-like protein functions in
RNA-directed DNA methylation and transcriptional gene silencing in Arabidopsis. Plant J. 62:92-99.
Fujii H, Chinnusamy V, Rodrigues A, Rubio S, Antoni R, Park SY, Cutler SR, Sheen J, Rodriguez PL, Zhu
JK. 2009. In vitro reconstitution of an abscisic acid signalling pathway. Nature 462:660-664.
He XJ, Hsu YF, Zhu S, Liu HL, Pontes O, Zhu J, Cui X, Wang CS, Zhu JK. 2009. A conserved
transcriptional regulator is required for RNA-directed DNA methylation and plant development. Genes
Dev. 23:2717-2722.
Melcher K, Ng LM, Zhou XE, Soon FF, Xu Y, Suino-Powell KM, Park SY, Weiner JJ, Fujii H, Chinnusamy
V, Kovach A, Li J, Wang Y, Li J, Peterson FC, Jensen DR, Yong EL, Volkman BF, Cutler SR, Zhu JK, Xu
HE. 2009. A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Nature
462:602-608.
Zhu JK. 2009. Active DNA Demethylation Mediated by DNA Glycosylases. Annu. Rev. Genet.
43:143-166.
Yang Q, Chen ZZ, Zhou XF, Yin HB, Li X, Xin XF, Hong XH, Zhu JK, Gong Z. 2009. Overexpression of
SOS (Salt Overly Sensitive) Genes Increases Salt Tolerance in Transgenic Arabidopsis. Mol. Plant
2:22-31.
He XJ, Hsu YF, Zhu S, Wierzbicki AT, Pontes O, Pikaard CS, Liu HL, Wang CS, Jin H, Zhu JK. 2009. An
effector of RNA-directed DNA methylation in Arabidopsis is an ARGONAUTE 4-and RNA-binding protein.
Cell 137:498-508.
Park SY, Fung P, Nishimura N, Jensen DR, Fujii H, Zhao Y, Lumba S, Santiago J, Rodrigues A, Chow TF,
Alfred SE, Bonetta D, Finkelstein R, Provart NJ, Desveaux D, Rodriquez PL, McCourt P, Zhu JK,
Schroeder JI, Volkman BR, Cutler SR. 2009. Abscisic acid inhibits type 2C protein phosphatases via the
PYR/PYL family of ABA binding START proteins. Science 324:1068-1071.
Fujii H, Zhu JK. 2009. Arabidopsis mutant deficient in three abscisic acid-activated protein kinases
reveals critical roles in growth, reproduction and stress. Proc. Natl. Acad. Sci. USA 106:8380-8685.
Bertorello AM, Zhu JK. 2009. SIK1/SOS2 networks: decoding sodium signals via calcium-responsive
protein kinase pathways. Pflugers Arch. 458:613-619.
He XJ, Hsu YF, Pontes O, Zhu J, Lu J, Bressan RA, Pikaard C, Wang CS, Zhu JK. 2009. NRPD4, a
protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerase IV and V
and is required for RNA-dicrected DNA methylation. Genes Dev. 23:318-330.
Chinnusamy V, Zhu JK. 2009. Epigenetic regulation of stress responses in plants. Curr. Opin. Plant Biol.
12:133-139.
Fujii H, Zhu JK. 2009. An autophosphorylation site of the protein kinase SOS2 is important for salt
tolerance in Arabidopsis. Mol. Plant. 2:183-190.
Dong CH, Zolman BK, Bartel B, Lee BH, Stevenson B, Agarwal M, Zhu JK. 2009. Disruption of
Arabidopsis CHY1 reveals an important role of metabolic status in plant cold stress signaling. Mol. Plant.
2:59-72.
Bressan R, Bohnert H, Zhu JK. 2009. Abiotic stress tolerance: from gene discovery in model organisms
to crop improvement. Mol. Plant. 2:1-2.