Eukaryotic Replication
Supplemental Instruction
Iowa State University
Leader:
Course:
Instructor:
Date:
Lilli Howard
BIOL/GEN 313
Dr. Rodermel/Dr. Tuggle
09/23/14
1. How does replication licensing ensure that DNA is replicated only once at each origin per
cell cycle?
2. In what ways is eukaryotic replication similar to bacterial replication, and in what ways is
it different?
3. What is the end-of-chromosome problem for replication? What is the solution? Why, in
the absence of telomerase, do the ends of chromosomes get progressively shorter each
time the DNA is replicated?
4. Outline in words and pictures how telomeres at the ends of eukaryotic chromosomes are
replicated.
5. ______________ must be relaxed for eukaryotic DNA replication.
6. Eukaryotic chromosomes contain multiple _____________________________________
to allow the genome to be replicated quickly.
7. _______________________________: a multisubunit protein complex; recognizes origin
and starts to unwind the DNA.
8. What are the three major DNA polymerases used in elongation? What actions do they
have?
1060 Hixson-Lied Student Success Center  515-294-6624  sistaff@iastate.edu  http://www.si.iastate.edu
9. Draw the cell cycle. In your diagram show the chromosome configuration at each of the
cell cycle stages for a species whose diploid number is 2 (2n = 2). Be sure and show
Watson and Crick strands, chromatids, chromosomes, centromeres. Indicate in each of
your diagrams the presence of MCM and/or geminin at a representative origin.
10. __________________________________________________: binds each origin in G1; it
serves as a tag for the replication machinery to initiate at this site (at the beginning of S).
11. What is geminin? Describe how it works.
12. Beyond a certain point of shortening, chromosomes become unstable and degrade: cell
death aka ___________________________.
13. What is the difference between a mouse that has over expression of a gene and a mouse
that has a knocked out gene? How are these used?
1060 Hixson-Lied Student Success Center  515-294-6624  sistaff@iastate.edu  http://www.si.iastate.edu