Laboratory Profile
Principal Investigator:
Batsheva Kerem
Abnormal DNA replication in cancer development-causes, effects and
therapeutic possibilities
Location: Silberman Wing 2, 5th floor Room 2-590
Communication:
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E.Mail: batshevak@mail.savion.huji.ac.il
Phone: 02-6585689
Web: http://www.bio.huji.ac.il/staff_in.asp?staff_id=64
Brief Description of Research:
Chromosomal instability is a hallmark of cancer. We have recently found that this
instability is caused by perturbed DNA replication, due to insufficient nucleotides
required to support normal DNA replication. Despite these new enlightening discoveries,
the molecular basis underlying the differences in DNA replication between normal and
tumor cells remains unknown. Moreover, the consequences of the replication
perturbation to the tumorigenic fate of the cells are still elusive. In the proposed project
we will explore how cancer genes and environmental factors lead to incoordination
between key genes/ pathways regulating DNA replication and cell proliferation.
Techniques used include:
The high resolution DNA combing technique which enables the analysis of DNA
replication on single DNA molecules, cytogenetic of fragile sites, expression arrays, SNP
and CNV arrays, immuneflorescence using specific antibodies. Cancer analysis in mouse
models.
Proposed Research Projects:
How does perturbed DNA replication affect cancer development?"