Characterization of the various Cu(II) and Cd(II) resistance mechanisms in Sphingobium sp.
PHE-SPH and Ochrobactrum sp. PHE-OCH, and their potential application in the
bioremediation of heavy metal-phenanthrene co-contaminated sites
Chen Chen1, Wenrui Lei1, Min Lu1, Chunling Luo2, Yahua Chen1, Zhenguo Shen1*
1
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People’s Republic of
China
2
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese
Academy of Sciences, No. 511 Kehua Street, Tianhe District, Guangzhou 510640, Guangdong Province,
China
* Corresponding author
Email: zgshen@njau.edu.cn
Tel: 0086- 025-84396391
Fax: 0086-025-84396391
Online Resource 1 FTIR analysis of Sphingobium sp. PHE-SPH and Ochrobactrum
sp. PHE-OCH grown with and without Cu(II) and Cd(II).
Bands (cm-1)
Control
Assignment
Functional type
References
Cells with Heavy metals
Cu(II)
Cd(II)
Sphingobium sp. PHE-SPH
1194
1186
1190
C–N stretching
Proteins
Mungasavalli et al. (2007)
1242
1242
1244
C=O Stretching
Carboxylic acids
Comte et al. (2006)
1333
1329
1329
C–H bending
1385
1404
1385
C=O Stretching
1481
1479
1479
C–H bending
1585
1583
1585
N–H bending (Amide II)
2926
2926
2924
C–H stretching
Wang & Sun (2013)
Carboxylic acids
Comte et al. (2006)
Poljanšek er al. (2006)
Proteins
Mungasavalli et al. (2007)
Park et al. (2005)
Ochrobactrum sp. PHE-OCH
1146
1146
1144
C–O–C stretching
Polysaccharides
Comte et al. (2006)
1186
1190
1188
C–N stretching
Proteins
Mungasavalli et al. (2007)
1240
1242
1242
C=O stretching
Carboxylic acids
Comte et al. (2006)
1348
1350
1348
C–H bending
Wang & Sun (2013)
1452
1454
1454
C–H bending (–CH2)
Guibaud et al. (2005)
1589
1589
1591
N–H bending (Amide II)
Proteins
Mungasavalli et al. (2007)
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